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Protein

Tripartite motif-containing protein 35

Gene

TRIM35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role as a tumor suppressor. Implicated in the cell death mechanism (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri21 – 6141RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri96 – 13742B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. innate immune response Source: UniProtKB
  3. negative regulation of cell proliferation Source: Ensembl
  4. negative regulation of mitotic cell cycle Source: UniProtKB
  5. negative regulation of viral release from host cell Source: UniProtKB
  6. positive regulation of apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_25078. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 35
Alternative name(s):
Hemopoietic lineage switch protein 5
Gene namesi
Name:TRIM35
Synonyms:HLS5, KIAA1098
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:16285. TRIM35.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleoplasm Source: HPA
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38115.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Tripartite motif-containing protein 35PRO_0000056250Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei4 – 41Phosphoserine1 Publication
Modified residuei8 – 81Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9UPQ4.
PaxDbiQ9UPQ4.
PRIDEiQ9UPQ4.

PTM databases

PhosphoSiteiQ9UPQ4.

Expressioni

Gene expression databases

BgeeiQ9UPQ4.
CleanExiHS_TRIM35.
ExpressionAtlasiQ9UPQ4. baseline and differential.
GenevestigatoriQ9UPQ4.

Organism-specific databases

HPAiHPA019647.

Interactioni

Protein-protein interaction databases

BioGridi116716. 14 interactions.
IntActiQ9UPQ4. 9 interactions.
STRINGi9606.ENSP00000301924.

Structurei

3D structure databases

ProteinModelPortaliQ9UPQ4.
SMRiQ9UPQ4. Positions 15-61, 101-243, 302-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini284 – 487204B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili210 – 25142Sequence AnalysisAdd
BLAST

Domaini

The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri21 – 6141RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri96 – 13742B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG285726.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000124586.
HOVERGENiHBG098569.
InParanoidiQ9UPQ4.
KOiK12012.
OMAiCWEVQVA.
OrthoDBiEOG74BJRP.
PhylomeDBiQ9UPQ4.
TreeFamiTF334286.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERSPDVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRGCVSRCWE
60 70 80 90 100
VQVSPTCPVC KDRASPADLR TNHTLNNLVE KLLREEAEGA RWTSYRFSRV
110 120 130 140 150
CRLHRGQLSL FCLEDKELLC CSCQADPRHQ GHRVQPVKDT AHDFRAKCRN
160 170 180 190 200
MEHALREKAK AFWAMRRSYE AIAKHNQVEA AWLEGRIRQE FDKLREFLRV
210 220 230 240 250
EEQAILDAMA EETRQKQLLA DEKMKQLTEE TEVLAHEIER LQMEMKEDDV
260 270 280 290 300
SFLMKHKSRK RRLFCTMEPE PVQPGMLIDV CKYLGSLQYR VWKKMLASVE
310 320 330 340 350
SVPFSFDPNT AAGWLSVSDD LTSVTNHGYR VQVENPERFS SAPCLLGSRV
360 370 380 390 400
FSQGSHAWEV ALGGLQSWRV GVVRVRQDSG AEGHSHSCYH DTRSGFWYVC
410 420 430 440 450
RTQGVEGDHC VTSDPATSPL VLAIPRRLRV ELECEEGELS FYDAERHCHL
460 470 480 490
YTFHARFGEV RPYFYLGGAR GAGPPEPLRI CPLHISVKEE LDG
Length:493
Mass (Da):56,540
Last modified:December 7, 2004 - v2
Checksum:iBFB7E5BFD3AD7E2D
GO
Isoform 2 (identifier: Q9UPQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-206: AKCRNMEHAL...FLRVEEQAIL → VRSLIAEERR...QGLLGHAALL

Note: No experimental confirmation available.

Show »
Length:493
Mass (Da):55,914
Checksum:i2DB3305B868B67F8
GO

Sequence cautioni

The sequence BAA83050.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei146 – 20661AKCRN…EQAIL → VRSLIAEERRNFLPTHQWIV TKTRLQTSSPNLQSRRQGQV QEHGACTAGEGQGLLGHAAL L in isoform 2. 1 PublicationVSP_012061Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492463 mRNA. Translation: AAO85480.1.
AB029021 mRNA. Translation: BAA83050.1. Different initiation.
BC018337 mRNA. Translation: AAH18337.1.
BC069226 mRNA. Translation: AAH69226.1.
CCDSiCCDS6056.2. [Q9UPQ4-1]
RefSeqiNP_741983.2. NM_171982.4. [Q9UPQ4-1]
UniGeneiHs.104223.

Genome annotation databases

EnsembliENST00000305364; ENSP00000301924; ENSG00000104228. [Q9UPQ4-1]
GeneIDi23087.
KEGGihsa:23087.
UCSCiuc003xfl.1. human. [Q9UPQ4-1]

Polymorphism databases

DMDMi56404980.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492463 mRNA. Translation: AAO85480.1.
AB029021 mRNA. Translation: BAA83050.1. Different initiation.
BC018337 mRNA. Translation: AAH18337.1.
BC069226 mRNA. Translation: AAH69226.1.
CCDSiCCDS6056.2. [Q9UPQ4-1]
RefSeqiNP_741983.2. NM_171982.4. [Q9UPQ4-1]
UniGeneiHs.104223.

3D structure databases

ProteinModelPortaliQ9UPQ4.
SMRiQ9UPQ4. Positions 15-61, 101-243, 302-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116716. 14 interactions.
IntActiQ9UPQ4. 9 interactions.
STRINGi9606.ENSP00000301924.

PTM databases

PhosphoSiteiQ9UPQ4.

Polymorphism databases

DMDMi56404980.

Proteomic databases

MaxQBiQ9UPQ4.
PaxDbiQ9UPQ4.
PRIDEiQ9UPQ4.

Protocols and materials databases

DNASUi23087.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305364; ENSP00000301924; ENSG00000104228. [Q9UPQ4-1]
GeneIDi23087.
KEGGihsa:23087.
UCSCiuc003xfl.1. human. [Q9UPQ4-1]

Organism-specific databases

CTDi23087.
GeneCardsiGC08M027142.
HGNCiHGNC:16285. TRIM35.
HPAiHPA019647.
neXtProtiNX_Q9UPQ4.
PharmGKBiPA38115.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG285726.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000124586.
HOVERGENiHBG098569.
InParanoidiQ9UPQ4.
KOiK12012.
OMAiCWEVQVA.
OrthoDBiEOG74BJRP.
PhylomeDBiQ9UPQ4.
TreeFamiTF334286.

Enzyme and pathway databases

ReactomeiREACT_25078. Interferon gamma signaling.

Miscellaneous databases

GenomeRNAii23087.
NextBioi44229.
PROiQ9UPQ4.

Gene expression databases

BgeeiQ9UPQ4.
CleanExiHS_TRIM35.
ExpressionAtlasiQ9UPQ4. baseline and differential.
GenevestigatoriQ9UPQ4.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor."
    Lalonde J.-P., Lim R., Ingley E., Tilbrook P.A., Thompson M.J., McCulloch R., Beaumont J.G., Wicking C., Eyre H.J., Sutherland G.R., Howe K., Solomon E., Williams J.H., Klinken S.P.
    J. Biol. Chem. 279:8181-8189(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  2. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Lung.
  4. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4 AND SER-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRI35_HUMAN
AccessioniPrimary (citable) accession number: Q9UPQ4
Secondary accession number(s): Q86XQ0, Q8WVA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: April 1, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.