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Q9UPQ4 (TRI35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tripartite motif-containing protein 35
Alternative name(s):
Hemopoietic lineage switch protein 5
Gene names
Name:TRIM35
Synonyms:HLS5, KIAA1098
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role as a tumor suppressor. Implicated in the cell death mechanism By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies By similarity.

Domain

The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity By similarity.

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 1 B box-type zinc finger.

Contains 1 B30.2/SPRY domain.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence BAA83050.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UPQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UPQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     146-206: AKCRNMEHAL...FLRVEEQAIL → VRSLIAEERR...QGLLGHAALL
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Tripartite motif-containing protein 35
PRO_0000056250

Regions

Domain284 – 487204B30.2/SPRY
Zinc finger21 – 6141RING-type
Zinc finger96 – 13742B box-type
Coiled coil210 – 25142 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.4
Modified residue41Phosphoserine Ref.4
Modified residue81Phosphoserine Ref.4

Natural variations

Alternative sequence146 – 20661AKCRN…EQAIL → VRSLIAEERRNFLPTHQWIV TKTRLQTSSPNLQSRRQGQV QEHGACTAGEGQGLLGHAAL L in isoform 2.
VSP_012061

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: BFB7E5BFD3AD7E2D

FASTA49356,540
        10         20         30         40         50         60 
MERSPDVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRGCVSRCWE VQVSPTCPVC 

        70         80         90        100        110        120 
KDRASPADLR TNHTLNNLVE KLLREEAEGA RWTSYRFSRV CRLHRGQLSL FCLEDKELLC 

       130        140        150        160        170        180 
CSCQADPRHQ GHRVQPVKDT AHDFRAKCRN MEHALREKAK AFWAMRRSYE AIAKHNQVEA 

       190        200        210        220        230        240 
AWLEGRIRQE FDKLREFLRV EEQAILDAMA EETRQKQLLA DEKMKQLTEE TEVLAHEIER 

       250        260        270        280        290        300 
LQMEMKEDDV SFLMKHKSRK RRLFCTMEPE PVQPGMLIDV CKYLGSLQYR VWKKMLASVE 

       310        320        330        340        350        360 
SVPFSFDPNT AAGWLSVSDD LTSVTNHGYR VQVENPERFS SAPCLLGSRV FSQGSHAWEV 

       370        380        390        400        410        420 
ALGGLQSWRV GVVRVRQDSG AEGHSHSCYH DTRSGFWYVC RTQGVEGDHC VTSDPATSPL 

       430        440        450        460        470        480 
VLAIPRRLRV ELECEEGELS FYDAERHCHL YTFHARFGEV RPYFYLGGAR GAGPPEPLRI 

       490 
CPLHISVKEE LDG 

« Hide

Isoform 2 [UniParc].

Checksum: 2DB3305B868B67F8
Show »

FASTA49355,914

References

« Hide 'large scale' references
[1]"HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor."
Lalonde J.-P., Lim R., Ingley E., Tilbrook P.A., Thompson M.J., McCulloch R., Beaumont J.G., Wicking C., Eyre H.J., Sutherland G.R., Howe K., Solomon E., Williams J.H., Klinken S.P.
J. Biol. Chem. 279:8181-8189(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[2]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Lung.
[4]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4 AND SER-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF492463 mRNA. Translation: AAO85480.1.
AB029021 mRNA. Translation: BAA83050.1. Different initiation.
BC018337 mRNA. Translation: AAH18337.1.
BC069226 mRNA. Translation: AAH69226.1.
CCDSCCDS6056.2. [Q9UPQ4-1]
RefSeqNP_741983.2. NM_171982.3. [Q9UPQ4-1]
UniGeneHs.104223.

3D structure databases

ProteinModelPortalQ9UPQ4.
SMRQ9UPQ4. Positions 15-61, 302-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116716. 13 interactions.
IntActQ9UPQ4. 9 interactions.
STRING9606.ENSP00000301924.

PTM databases

PhosphoSiteQ9UPQ4.

Polymorphism databases

DMDM56404980.

Proteomic databases

PaxDbQ9UPQ4.
PRIDEQ9UPQ4.

Protocols and materials databases

DNASU23087.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305364; ENSP00000301924; ENSG00000104228. [Q9UPQ4-1]
GeneID23087.
KEGGhsa:23087.
UCSCuc003xfl.1. human. [Q9UPQ4-1]

Organism-specific databases

CTD23087.
GeneCardsGC08M027142.
HGNCHGNC:16285. TRIM35.
HPAHPA019647.
neXtProtNX_Q9UPQ4.
PharmGKBPA38115.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG285726.
HOGENOMHOG000124586.
HOVERGENHBG098569.
InParanoidQ9UPQ4.
KOK12012.
OMACWEVQVA.
OrthoDBEOG74BJRP.
PhylomeDBQ9UPQ4.
TreeFamTF334286.

Gene expression databases

ArrayExpressQ9UPQ4.
BgeeQ9UPQ4.
CleanExHS_TRIM35.
GenevestigatorQ9UPQ4.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR006574. PRY.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi23087.
NextBio44229.
PROQ9UPQ4.

Entry information

Entry nameTRI35_HUMAN
AccessionPrimary (citable) accession number: Q9UPQ4
Secondary accession number(s): Q86XQ0, Q8WVA4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM