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Protein

Tripartite motif-containing protein 35

Gene

TRIM35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (PubMed:25263439). Involved in the cell death mechanism (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 61RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri96 – 137B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • innate immune response Source: UniProtKB
  • negative regulation of mitotic cell cycle Source: UniProtKB
  • negative regulation of viral release from host cell Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104228-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 35
Alternative name(s):
Hemopoietic lineage switch protein 5
Gene namesi
Name:TRIM35
Synonyms:HLS5, KIAA1098
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:16285. TRIM35.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus By similarity

  • Note: Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi23087.
OpenTargetsiENSG00000104228.
PharmGKBiPA38115.

Polymorphism and mutation databases

BioMutaiTRIM35.
DMDMi56404980.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562501 – 493Tripartite motif-containing protein 35Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei4PhosphoserineCombined sources1
Modified residuei8PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UPQ4.
PaxDbiQ9UPQ4.
PeptideAtlasiQ9UPQ4.
PRIDEiQ9UPQ4.

PTM databases

iPTMnetiQ9UPQ4.
PhosphoSitePlusiQ9UPQ4.

Expressioni

Gene expression databases

BgeeiENSG00000104228.
CleanExiHS_TRIM35.
ExpressionAtlasiQ9UPQ4. baseline and differential.
GenevisibleiQ9UPQ4. HS.

Organism-specific databases

HPAiHPA019647.

Interactioni

Subunit structurei

Interacts with PKM isoform M2, but not isoform M1; this interaction may compete with that between PKM and FGFR1, and hence reduces FGFR1-dependent tyrosine phosphorylation of PKM.1 Publication

Protein-protein interaction databases

BioGridi116716. 33 interactors.
IntActiQ9UPQ4. 9 interactors.
STRINGi9606.ENSP00000301924.

Structurei

3D structure databases

ProteinModelPortaliQ9UPQ4.
SMRiQ9UPQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini284 – 487B30.2/SPRYPROSITE-ProRule annotationAdd BLAST204

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili210 – 251Sequence analysisAdd BLAST42

Domaini

The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 61RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri96 – 137B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFW. Eukaryota.
ENOG410YB4K. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000124586.
HOVERGENiHBG098569.
InParanoidiQ9UPQ4.
KOiK12012.
OMAiCWEVQVA.
OrthoDBiEOG091G0AKN.
PhylomeDBiQ9UPQ4.
TreeFamiTF334286.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERSPDVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRGCVSRCWE
60 70 80 90 100
VQVSPTCPVC KDRASPADLR TNHTLNNLVE KLLREEAEGA RWTSYRFSRV
110 120 130 140 150
CRLHRGQLSL FCLEDKELLC CSCQADPRHQ GHRVQPVKDT AHDFRAKCRN
160 170 180 190 200
MEHALREKAK AFWAMRRSYE AIAKHNQVEA AWLEGRIRQE FDKLREFLRV
210 220 230 240 250
EEQAILDAMA EETRQKQLLA DEKMKQLTEE TEVLAHEIER LQMEMKEDDV
260 270 280 290 300
SFLMKHKSRK RRLFCTMEPE PVQPGMLIDV CKYLGSLQYR VWKKMLASVE
310 320 330 340 350
SVPFSFDPNT AAGWLSVSDD LTSVTNHGYR VQVENPERFS SAPCLLGSRV
360 370 380 390 400
FSQGSHAWEV ALGGLQSWRV GVVRVRQDSG AEGHSHSCYH DTRSGFWYVC
410 420 430 440 450
RTQGVEGDHC VTSDPATSPL VLAIPRRLRV ELECEEGELS FYDAERHCHL
460 470 480 490
YTFHARFGEV RPYFYLGGAR GAGPPEPLRI CPLHISVKEE LDG
Length:493
Mass (Da):56,540
Last modified:December 7, 2004 - v2
Checksum:iBFB7E5BFD3AD7E2D
GO
Isoform 2 (identifier: Q9UPQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-206: AKCRNMEHAL...FLRVEEQAIL → VRSLIAEERR...QGLLGHAALL

Note: No experimental confirmation available.
Show »
Length:493
Mass (Da):55,914
Checksum:i2DB3305B868B67F8
GO

Sequence cautioni

The sequence BAA83050 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012061146 – 206AKCRN…EQAIL → VRSLIAEERRNFLPTHQWIV TKTRLQTSSPNLQSRRQGQV QEHGACTAGEGQGLLGHAAL L in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492463 mRNA. Translation: AAO85480.1.
AB029021 mRNA. Translation: BAA83050.1. Different initiation.
BC018337 mRNA. Translation: AAH18337.1.
BC069226 mRNA. Translation: AAH69226.1.
CCDSiCCDS6056.2. [Q9UPQ4-1]
RefSeqiNP_741983.2. NM_171982.4. [Q9UPQ4-1]
UniGeneiHs.104223.

Genome annotation databases

EnsembliENST00000305364; ENSP00000301924; ENSG00000104228. [Q9UPQ4-1]
GeneIDi23087.
KEGGihsa:23087.
UCSCiuc003xfl.2. human. [Q9UPQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492463 mRNA. Translation: AAO85480.1.
AB029021 mRNA. Translation: BAA83050.1. Different initiation.
BC018337 mRNA. Translation: AAH18337.1.
BC069226 mRNA. Translation: AAH69226.1.
CCDSiCCDS6056.2. [Q9UPQ4-1]
RefSeqiNP_741983.2. NM_171982.4. [Q9UPQ4-1]
UniGeneiHs.104223.

3D structure databases

ProteinModelPortaliQ9UPQ4.
SMRiQ9UPQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116716. 33 interactors.
IntActiQ9UPQ4. 9 interactors.
STRINGi9606.ENSP00000301924.

PTM databases

iPTMnetiQ9UPQ4.
PhosphoSitePlusiQ9UPQ4.

Polymorphism and mutation databases

BioMutaiTRIM35.
DMDMi56404980.

Proteomic databases

EPDiQ9UPQ4.
PaxDbiQ9UPQ4.
PeptideAtlasiQ9UPQ4.
PRIDEiQ9UPQ4.

Protocols and materials databases

DNASUi23087.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305364; ENSP00000301924; ENSG00000104228. [Q9UPQ4-1]
GeneIDi23087.
KEGGihsa:23087.
UCSCiuc003xfl.2. human. [Q9UPQ4-1]

Organism-specific databases

CTDi23087.
DisGeNETi23087.
GeneCardsiTRIM35.
HGNCiHGNC:16285. TRIM35.
HPAiHPA019647.
MIMi617007. gene.
neXtProtiNX_Q9UPQ4.
OpenTargetsiENSG00000104228.
PharmGKBiPA38115.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITFW. Eukaryota.
ENOG410YB4K. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000124586.
HOVERGENiHBG098569.
InParanoidiQ9UPQ4.
KOiK12012.
OMAiCWEVQVA.
OrthoDBiEOG091G0AKN.
PhylomeDBiQ9UPQ4.
TreeFamiTF334286.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104228-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.

Miscellaneous databases

GenomeRNAii23087.
PROiQ9UPQ4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104228.
CleanExiHS_TRIM35.
ExpressionAtlasiQ9UPQ4. baseline and differential.
GenevisibleiQ9UPQ4. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI35_HUMAN
AccessioniPrimary (citable) accession number: Q9UPQ4
Secondary accession number(s): Q86XQ0, Q8WVA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.