Q9UPN7 (PP6R1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 6 regulatory subunit 1

Alternative name(s):
SAPS domain family member 1

Gene names

Name:	PPP6R1
Synonyms:	KIAA1115, PP6R1, SAPS1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Ref.4
Subunit structure	Protein phospatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed of the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with PPP6C and NFKBIE. Interacts with ANKRD28, ANKRD44 and ANKRD52. Ref.4 Ref.5
Subcellular location	Cytoplasm Ref.4 Ref.5.
Tissue specificity	Ubiquitous with higher expression in testis. Ref.4
Sequence similarities	Belongs to the SAPS family.
Sequence caution	The sequence BAA83067.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of phosphoprotein phosphatase activity Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	protein phosphatase binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANKRD28	O15084	4	EBI-359745,EBI-359567
PPP6C	O00743	5	EBI-359745,EBI-359751

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	Serine/threonine-protein phosphatase 6 regulatory subunit 1		PRO_0000046096
Regions
	Region	10 – 403	394	Interaction with PPP6C
	Compositional bias	624 – 685	62	Glu-rich
	Compositional bias	624 – 631	8	Poly-Glu
	Compositional bias	671 – 685	15	Poly-Glu
	Compositional bias	697 – 878	182	Pro-rich
Amino acid modifications
	Modified residue	529	1	Phosphoserine By similarity
	Modified residue	530	1	Phosphoserine By similarity
	Modified residue	531	1	Phosphoserine By similarity
	Modified residue	635	1	Phosphoserine Ref.7 Ref.9
	Modified residue	638	1	Phosphoserine Ref.7 Ref.9
	Modified residue	664	1	Phosphoserine Ref.7
	Modified residue	667	1	Phosphoserine Ref.7
	Modified residue	668	1	Phosphothreonine Ref.7
	Modified residue	670	1	Phosphoserine Ref.7
	Modified residue	702	1	Phosphoserine Ref.9
	Modified residue	726	1	Phosphoserine Ref.9
	Modified residue	759	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9
	Modified residue	846	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UPN7 [UniParc]. Last modified October 13, 2009. Version 5. Checksum: 36DDA2E945ED599A	FASTA	881	96,724
	10 20 30 40 50 60 MFWKFDLHTS SHLDTLLERE DLSLPELLDE EDVLQECKVV NRKLLDFLLQ PPHLQAMVAW 70 80 90 100 110 120 VTQEPPDSGE ERLRYKYPSV ACEILTSDVP QINDALGADE SLLNRLYGFL QSTGSLNPLL 130 140 150 160 170 180 ASFFSKVMGI LINRKTDQLV SFLRKKDDFV DLLLQHIGTS AIMDLLLRLL TCVERPQLRQ 190 200 210 220 230 240 DVVNWLNEEK IVQRLIEQIH PSKDENQHSN ASQSLCDIIR LSREQMIQVQ DSPEPDQLLA 250 260 270 280 290 300 TLEKQETIEQ LLSNMFEGEQ SQSVIVSGIQ VLLTLLEPRR PRSESVTVNS FFSSVDGQLE 310 320 330 340 350 360 LLAQGALEST VSSVGALHAL RPRLSCFHQL LLEPPKLEPL QMTWGMLAPP LGNTRLHVVK 370 380 390 400 410 420 LLASALSAND AALTHELLAL DVPNTMLDLF FHYVFNNFLH AQVEGCVSTM LSLGPPPDSS 430 440 450 460 470 480 PETPIQNPVV KHLLQQCRLV ERILTSWEEN DRVQCAGGPR KGYMGHLTRV AGALVQNTEK 490 500 510 520 530 540 GPNAEQLRQL LKELPSEQQE QWEAFVSGPL AETNKKNMVD LVNTHHLHSS SDDEDDRLKE 550 560 570 580 590 600 FNFPEEAVLQ QAFMDFQMQR MTSAFIDHFG FNDEEFGEQE ESVNAPFDKT ANITFSLNAD 610 620 630 640 650 660 DENPNANLLE ICYKDRIQQF DDDEEEEDEE EAQGSGESDG EDGAWQGSQL ARGARLGQPP 670 680 690 700 710 720 GVRSGGSTDS EDEEEEDEEE EEDEEGIGCA ARGGATPLSY PSPGPQPPGP SWTATFDPVP 730 740 750 760 770 780 TDAPTSPRVS GEEELHTGPP APQGPLSVPQ GLPTQSLASP PARDALQLRS QDPTPPSAPQ 790 800 810 820 830 840 EATEGSKVTE PSAPCQALVS IGDLQATFHG IRSAPSSSDS ATRDPSTSVP ASGAHQPPQT 850 860 870 880 TEGEKSPEPL GLPQSQSAQA LTPPPIPNGS APEGPASPGS Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed: 10470851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	Ohara O., Nagase T., Kikuno R. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph, Skin and Testis.
[4]	"Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon." Stefansson B., Brautigan D.L. J. Biol. Chem. 281:22624-22634(2006) [PubMed: 16769727] [Abstract] Cited for: FUNCTION, INTERACTION WITH PPP6C AND NFKBIE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains." Stefansson B., Ohama T., Daugherty A.E., Brautigan D.L. Biochemistry 47:1442-1451(2008) [PubMed: 18186651] [Abstract] Cited for: INTERACTION WITH PPP6C; ANKRD28; ANKRD44 AND ANKRD52, SUBCELLULAR LOCATION.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-759, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-638; SER-664; SER-667; THR-668; SER-670 AND SER-759, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-759, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-638; SER-702; SER-726 AND SER-759, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB029038 mRNA. Translation: BAA83067.3. Different initiation. BC002799 mRNA. Translation: AAH02799.2. BC068014 mRNA. Translation: AAH68014.1. BC094753 mRNA. Translation: AAH94753.2.
IPI	IPI00873586.
RefSeq	NP_055746.3. NM_014931.3.
UniGene	Hs.515610.
3D structure databases
ProteinModelPortal	Q9UPN7.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27589N.
IntAct	Q9UPN7. 8 interactions.
MINT	MINT-1154650.
STRING	Q9UPN7.
PTM databases
PhosphoSite	Q9UPN7.
Polymorphism databases
DMDM	261260102.
Proteomic databases
PRIDE	Q9UPN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000443936; ENSP00000390608; ENSG00000105063.
GeneID	22870.
KEGG	hsa:22870.
UCSC	uc002qjv.1. human. uc002qjw.2. human.
Organism-specific databases
CTD	22870.
GeneCards	GC19M055742.
HGNC	HGNC:29195. PPP6R1.
HPA	HPA043989.
MIM	610875. gene.
neXtProt	NX_Q9UPN7.
PharmGKB	PA128394589. PA165394108.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04482.
GeneTree	ENSGT00390000009899.
HOGENOM	HBG505370.
HOVERGEN	HBG069733.
InParanoid	Q9UPN7.
OrthoDB	EOG4P2Q1M.
PhylomeDB	Q9UPN7.
Gene expression databases
ArrayExpress	Q9UPN7.
Bgee	Q9UPN7.
CleanEx	HS_SAPS1.
Genevestigator	Q9UPN7.
GermOnline	ENSG00000105063. Homo sapiens.
Family and domain databases
InterPro	IPR007587. SAPS. [Graphical view]
KO	K15499.
PANTHER	PTHR12634. SAPS. 1 hit.
Pfam	PF04499. SAPS. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	43399.
PMAP-CutDB	Q9UPN7.
SOURCE	Search...

Entry information

Entry name

PP6R1_HUMAN

Accession

Primary (citable) accession number: Q9UPN7
Secondary accession number(s): Q2M2H3 , Q504V2, Q6NVJ6, Q9BU97

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 13, 2009
Last modified:	January 25, 2012
This is version 96 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.