Reviewed, UniProtKB/Swiss-Prot Q9UP79 (ATS8_HUMAN)

Names and origin

Protein names

Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 8
      Short name=ADAMTS-8
      Short name=ADAM-TS 8
      Short name=ADAM-TS8
    EC=3.4.24.-
Alternative name(s):
    METH-2
    METH-8

Gene names

Name:	ADAMTS8
Synonyms:	METH2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has anti-angiogenic properties.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Highly expressed in adult and fetal lung, lower expression in brain, placenta, heart, stomach and fetal brain and kidney.
Domain	The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 2 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Repeat Signal
Ligand	Heparin-binding Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Ref.1 Traceable author statement. Source: ProtInc proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW integrin binding Ref.1 Traceable author statement. Source: ProtInc low affinity phosphate transmembrane transporter activity Ref.2 Traceable author statement. Source: ProtInc metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 214	187	By similarity		PRO_0000029178
	Chain	215 – 890	676	A disintegrin and metalloproteinase with thrombospondin motifs 8		PRO_0000029179
Regions
	Domain	220 – 430	211	Peptidase M12B
	Domain	439 – 526	88	Disintegrin
	Domain	527 – 582	56	TSP type-1 1
	Domain	834 – 889	56	TSP type-1 2
	Region	691 – 832	142	Spacer
	Compositional bias	202 – 205	4	Poly-Pro
	Compositional bias	584 – 690	107	Cys-rich
Sites
	Active site	365	1	By similarity
	Metal binding	364	1	Zinc; catalytic By similarity
	Metal binding	368	1	Zinc; catalytic By similarity
	Metal binding	374	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	255	1	Phosphotyrosine Ref.3
	Modified residue	259	1	Phosphoserine Ref.3
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
	Glycosylation	600	1	N-linked (GlcNAc...) Potential
	Disulfide bond	342 ↔ 425		By similarity
	Disulfide bond	380 ↔ 409		By similarity
	Disulfide bond	539 ↔ 576		By similarity
	Disulfide bond	543 ↔ 581		By similarity
	Disulfide bond	554 ↔ 566		By similarity
Experimental info
	Sequence conflict	195	1	E → R in AAF25806. Ref.2
	Sequence conflict	413 – 440	28	YLTEL…LPTGL → FSGCHLQGWIHFKYLCKCVS ELKCDLMP in AAF25806. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UP79-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 57D70EE03D5739D3	FASTA	890	96,672
	10 20 30 40 50 60 MFPAPAAPRW LPFLLLLLLL LLPLARGAPA RPAAGGQASE LVVPTRLPGS AGELALHLSA 70 80 90 100 110 120 FGKGFVLRLA PDDSFLAPEF KIERLGGSGR ATGGERGLRG CFFSGTVNGE PESLAAVSLC 130 140 150 160 170 180 RGLSGSFLLD GEEFTIQPQG AGGSLAQPHR LQRWGPAGAR PLPRGPEWEV ETGEGQRQER 190 200 210 220 230 240 GDHQEDSEEE SQEEEAEGAS EPPPPLGATS RTKRFVSEAR FVETLLVADA SMAAFYGADL 250 260 270 280 290 300 QNHILTLMSV AARIYKHPSI KNSINLMVVK VLIVEDEKWG PEVSDNGGLT LRNFCNWQRR 310 320 330 340 350 360 FNQPSDRHPE HYDTAILLTR QNFCGQEGLC DTLGVADIGT ICDPNKSCSV IEDEGLQAAH 370 380 390 400 410 420 TLAHELGHVL SMPHDDSKPC TRLFGPMGKH HVMAPLFVHL NQTLPWSPCS AMYLTELLDG 430 440 450 460 470 480 GHGDCLLDAP GAALPLPTGL PGRMALYQLD QQCRQIFGPD FRHCPNTSAQ DVCAQLWCHT 490 500 510 520 530 540 DGAEPLCHTK NGSLPWADGT PCGPGHLCSE GSCLPEEEVE RPKPVVDGGW APWGPWGECS 550 560 570 580 590 600 RTCGGGVQFS HRECKDPEPQ NGGRYCLGRR AKYQSCHTEE CPPDGKSFRE QQCEKYNAYN 610 620 630 640 650 660 YTDMDGNLLQ WVPKYAGVSP RDRCKLFCRA RGRSEFKVFE AKVIDGTLCG PETLAICVRG 670 680 690 700 710 720 QCVKAGCDHV VDSPRKLDKC GVCGGKGNSC RKVSGSLTPT NYGYNDIVTI PAGATNIDVK 730 740 750 760 770 780 QRSHPGVQND GNYLALKTAD GQYLLNGNLA ISAIEQDILV KGTILKYSGS IATLERLQSF 790 800 810 820 830 840 RPLPEPLTVQ LLTVPGEVFP PKVKYTFFVP NDVDFSMQSS KERATTNIIQ PLLHAQWVLG 850 860 870 880 890 DWSECSSTCG AGWQRRTVEC RDPSGQASAT CNKALKPEDA KPCESQLCPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity." Vazquez F., Hastings G., Ortega M.-A., Lane T.F., Oikemus S., Lombardo M., Iruela-Arispe M.L. J. Biol. Chem. 274:23349-23357(1999) [PubMed: 10438512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11." Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S. Genomics 62:312-315(1999) [PubMed: 10610729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 195-440.
[3]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-255 AND SER-259, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF060153 mRNA. Translation: AAD48081.1. AF175283 mRNA. Translation: AAF25806.1.
IPI	IPI00298650.
UniGene	Hs.271605
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UP79.
Proteomic databases
PRIDE	Q9UP79.
Genome annotation databases
Ensembl	ENST00000257359; ENSP00000257359; ENSG00000134917; Homo sapiens. [Genome view]
UCSC	uc001qgg.2. human.
Organism-specific databases
GeneCards	GC11M129780.
H-InvDB	HIX0026131.
HGNC	HGNC:224. ADAMTS8.
MIM	605175. gene.
PharmGKB	PA24552.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9UP79.
InParanoid	Q9UP79.
Gene expression databases
ArrayExpress	Q9UP79.
Bgee	Q9UP79.
CleanEx	HS_ADAMTS8.
Genevestigator	Q9UP79.
Family and domain databases
InterPro	IPR010294. ADAM_spacer1. IPR013277. Pept_M12B_ADAM-TS8. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF05986. ADAM_spacer1. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 2 hits. [Graphical view]
PRINTS	PR01861. ADAMTS8. PR01857. ADAMTSFAMILY.
SMART	SM00209. TSP1. 2 hits. [Graphical view]
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. False negative. PS50214. DISINTEGRIN_2. False negative. PS50092. TSP1. 2 hits. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ATS8_HUMAN

Accession

Primary (citable) accession number: Q9UP79
Secondary accession number(s): Q9NZS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 15, 2009
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.