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Q9UP79

- ATS8_HUMAN

UniProt

Q9UP79 - ATS8_HUMAN

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Protein
A disintegrin and metalloproteinase with thrombospondin motifs 8
Gene
ADAMTS8, METH2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Has anti-angiogenic properties.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi363 – 3631Zinc; catalytic By similarity
Active sitei364 – 3641 By similarity
Metal bindingi367 – 3671Zinc; catalytic By similarity
Metal bindingi373 – 3731Zinc; catalytic By similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. integrin binding Source: ProtInc
  3. low affinity phosphate transmembrane transporter activity Source: ProtInc
  4. metalloendopeptidase activity Source: InterPro
  5. metallopeptidase activity Source: ProtInc
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of cell proliferation Source: ProtInc
  2. phosphate ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Heparin-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_118572. Degradation of the extracellular matrix.
REACT_200626. O-glycosylation of TSR domain-containing proteins.
REACT_201925. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiM12.226.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8 (EC:3.4.24.-)
Short name:
ADAM-TS 8
Short name:
ADAM-TS8
Short name:
ADAMTS-8
Alternative name(s):
METH-2
METH-8
Gene namesi
Name:ADAMTS8
Synonyms:METH2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:224. ADAMTS8.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Propeptidei27 – 213187 By similarity
PRO_0000029178Add
BLAST
Chaini214 – 889676A disintegrin and metalloproteinase with thrombospondin motifs 8
PRO_0000029179Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi294 ↔ 347 By similarity
Disulfide bondi323 ↔ 329 By similarity
Disulfide bondi341 ↔ 424 By similarity
Glycosylationi344 – 3441N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi379 ↔ 408 By similarity
Glycosylationi400 – 4001N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi452 ↔ 477 By similarity
Disulfide bondi463 ↔ 486 By similarity
Glycosylationi465 – 4651N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi472 ↔ 507 By similarity
Glycosylationi490 – 4901N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi501 ↔ 512 By similarity
Disulfide bondi538 ↔ 575 By similarity
Disulfide bondi542 ↔ 580 By similarity
Disulfide bondi553 ↔ 565 By similarity
Glycosylationi599 – 5991N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase By similarity.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9UP79.
PRIDEiQ9UP79.

PTM databases

PhosphoSiteiQ9UP79.

Expressioni

Tissue specificityi

Highly expressed in adult and fetal lung, lower expression in brain, placenta, heart, stomach and fetal brain and kidney.

Gene expression databases

BgeeiQ9UP79.
CleanExiHS_ADAMTS8.
GenevestigatoriQ9UP79.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000257359.

Structurei

3D structure databases

ProteinModelPortaliQ9UP79.
SMRiQ9UP79. Positions 219-809.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini219 – 429211Peptidase M12B
Add
BLAST
Domaini438 – 52588Disintegrin
Add
BLAST
Domaini526 – 58156TSP type-1 1
Add
BLAST
Domaini833 – 88856TSP type-1 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni690 – 831142Spacer
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi201 – 2044Poly-Pro
Compositional biasi583 – 689107Cys-rich
Add
BLAST

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Sequence similaritiesi

Contains 1 disintegrin domain.
Contains 2 TSP type-1 domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG285519.
HOGENOMiHOG000004799.
HOVERGENiHBG004313.
InParanoidiQ9UP79.
KOiK08623.
OMAiYELDQQC.
OrthoDBiEOG7WDN1M.
PhylomeDBiQ9UP79.
TreeFamiTF331949.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSiPR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 2 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UP79-1 [UniParc]FASTAAdd to Basket

« Hide

MLPAPAAPRW PPLLLLLLLL LPLARGAPAR PAAGGQASEL VVPTRLPGSA    50
GELALHLSAF GKGFVLRLAP DDSFLAPEFK IERLGGSGRA TGGERGLRGC 100
FFSGTVNGEP ESLAAVSLCR GLSGSFLLDG EEFTIQPQGA GGSLAQPHRL 150
QRWGPAGARP LPRGPEWEVE TGEGQRQERG DHQEDSEEES QEEEAEGASE 200
PPPPLGATSR TKRFVSEARF VETLLVADAS MAAFYGADLQ NHILTLMSVA 250
ARIYKHPSIK NSINLMVVKV LIVEDEKWGP EVSDNGGLTL RNFCNWQRRF 300
NQPSDRHPEH YDTAILLTRQ NFCGQEGLCD TLGVADIGTI CDPNKSCSVI 350
EDEGLQAAHT LAHELGHVLS MPHDDSKPCT RLFGPMGKHH VMAPLFVHLN 400
QTLPWSPCSA MYLTELLDGG HGDCLLDAPA AALPLPTGLP GRMALYQLDQ 450
QCRQIFGPDF RHCPNTSAQD VCAQLWCHTD GAEPLCHTKN GSLPWADGTP 500
CGPGHLCSEG SCLPEEEVER PKPVADGGWA PWGPWGECSR TCGGGVQFSH 550
RECKDPEPQN GGRYCLGRRA KYQSCHTEEC PPDGKSFREQ QCEKYNAYNY 600
TDMDGNLLQW VPKYAGVSPR DRCKLFCRAR GRSEFKVFEA KVIDGTLCGP 650
ETLAICVRGQ CVKAGCDHVV DSPRKLDKCG VCGGKGNSCR KVSGSLTPTN 700
YGYNDIVTIP AGATNIDVKQ RSHPGVQNDG NYLALKTADG QYLLNGNLAI 750
SAIEQDILVK GTILKYSGSI ATLERLQSFR PLPEPLTVQL LTVPGEVFPP 800
KVKYTFFVPN DVDFSMQSSK ERATTNIIQP LLHAQWVLGD WSECSSTCGA 850
GWQRRTVECR DPSGQASATC NKALKPEDAK PCESQLCPL 889
Length:889
Mass (Da):96,460
Last modified:November 30, 2010 - v2
Checksum:iE1767A6524BCEBAB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21L → F in AAD48081. 1 Publication
Sequence conflicti11 – 122PP → LPF in AAD48081. 1 Publication
Sequence conflicti194 – 1941E → R in AAF25806. 1 Publication
Sequence conflicti412 – 43928YLTEL…LPTGL → FSGCHLQGWIHFKYLCKCVS ELKCDLMP in AAF25806. 1 Publication
Add
BLAST
Sequence conflicti430 – 4301A → G in AAD48081. 1 Publication
Sequence conflicti525 – 5251A → V in AAD48081. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060153 mRNA. Translation: AAD48081.1.
AP002986 Genomic DNA. No translation available.
AF175283 mRNA. Translation: AAF25806.1.
CCDSiCCDS41732.1.
RefSeqiNP_008968.4. NM_007037.4.
XP_006718821.1. XM_006718758.1.
UniGeneiHs.271605.

Genome annotation databases

EnsembliENST00000257359; ENSP00000257359; ENSG00000134917.
GeneIDi11095.
KEGGihsa:11095.
UCSCiuc001qgg.4. human.

Polymorphism databases

DMDMi313104077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060153 mRNA. Translation: AAD48081.1 .
AP002986 Genomic DNA. No translation available.
AF175283 mRNA. Translation: AAF25806.1 .
CCDSi CCDS41732.1.
RefSeqi NP_008968.4. NM_007037.4.
XP_006718821.1. XM_006718758.1.
UniGenei Hs.271605.

3D structure databases

ProteinModelPortali Q9UP79.
SMRi Q9UP79. Positions 219-809.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000257359.

Protein family/group databases

MEROPSi M12.226.

PTM databases

PhosphoSitei Q9UP79.

Polymorphism databases

DMDMi 313104077.

Proteomic databases

PaxDbi Q9UP79.
PRIDEi Q9UP79.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000257359 ; ENSP00000257359 ; ENSG00000134917 .
GeneIDi 11095.
KEGGi hsa:11095.
UCSCi uc001qgg.4. human.

Organism-specific databases

CTDi 11095.
GeneCardsi GC11M130308.
H-InvDB HIX0026131.
HGNCi HGNC:224. ADAMTS8.
MIMi 605175. gene.
neXtProti NX_Q9UP79.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG285519.
HOGENOMi HOG000004799.
HOVERGENi HBG004313.
InParanoidi Q9UP79.
KOi K08623.
OMAi YELDQQC.
OrthoDBi EOG7WDN1M.
PhylomeDBi Q9UP79.
TreeFami TF331949.

Enzyme and pathway databases

Reactomei REACT_118572. Degradation of the extracellular matrix.
REACT_200626. O-glycosylation of TSR domain-containing proteins.
REACT_201925. Degradation of the extracellular matrix.

Miscellaneous databases

GeneWikii ADAMTS8.
GenomeRNAii 11095.
NextBioi 42178.
PROi Q9UP79.
SOURCEi Search...

Gene expression databases

Bgeei Q9UP79.
CleanExi HS_ADAMTS8.
Genevestigatori Q9UP79.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view ]
Pfami PF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view ]
PRINTSi PR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTi SM00209. TSP1. 2 hits.
[Graphical view ]
SUPFAMi SSF82895. SSF82895. 2 hits.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity."
    Vazquez F., Hastings G., Ortega M.-A., Lane T.F., Oikemus S., Lombardo M., Iruela-Arispe M.L.
    J. Biol. Chem. 274:23349-23357(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11."
    Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S.
    Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-439.

Entry informationi

Entry nameiATS8_HUMAN
AccessioniPrimary (citable) accession number: Q9UP79
Secondary accession number(s): Q9NZS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 30, 2010
Last modified: September 3, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi