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Q9UP79 (ATS8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8

Short name=ADAM-TS 8
Short name=ADAM-TS8
Short name=ADAMTS-8
EC=3.4.24.-
Alternative name(s):
METH-2
METH-8
Gene names
Name:ADAMTS8
Synonyms:METH2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has anti-angiogenic properties.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Highly expressed in adult and fetal lung, lower expression in brain, placenta, heart, stomach and fetal brain and kidney.

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 2 TSP type-1 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 213187 By similarity
PRO_0000029178
Chain214 – 889676A disintegrin and metalloproteinase with thrombospondin motifs 8
PRO_0000029179

Regions

Domain219 – 429211Peptidase M12B
Domain438 – 52588Disintegrin
Domain526 – 58156TSP type-1 1
Domain833 – 88856TSP type-1 2
Region690 – 831142Spacer
Compositional bias201 – 2044Poly-Pro
Compositional bias583 – 689107Cys-rich

Sites

Active site3641 By similarity
Metal binding3631Zinc; catalytic By similarity
Metal binding3671Zinc; catalytic By similarity
Metal binding3731Zinc; catalytic By similarity

Amino acid modifications

Glycosylation3441N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation4901N-linked (GlcNAc...) Potential
Glycosylation5991N-linked (GlcNAc...) Potential
Disulfide bond294 ↔ 347 By similarity
Disulfide bond323 ↔ 329 By similarity
Disulfide bond341 ↔ 424 By similarity
Disulfide bond379 ↔ 408 By similarity
Disulfide bond452 ↔ 477 By similarity
Disulfide bond463 ↔ 486 By similarity
Disulfide bond472 ↔ 507 By similarity
Disulfide bond501 ↔ 512 By similarity
Disulfide bond538 ↔ 575 By similarity
Disulfide bond542 ↔ 580 By similarity
Disulfide bond553 ↔ 565 By similarity

Experimental info

Sequence conflict21L → F in AAD48081. Ref.1
Sequence conflict11 – 122PP → LPF in AAD48081. Ref.1
Sequence conflict1941E → R in AAF25806. Ref.3
Sequence conflict412 – 43928YLTEL…LPTGL → FSGCHLQGWIHFKYLCKCVS ELKCDLMP in AAF25806. Ref.3
Sequence conflict4301A → G in AAD48081. Ref.1
Sequence conflict5251A → V in AAD48081. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9UP79 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: E1767A6524BCEBAB

FASTA88996,460
        10         20         30         40         50         60 
MLPAPAAPRW PPLLLLLLLL LPLARGAPAR PAAGGQASEL VVPTRLPGSA GELALHLSAF 

        70         80         90        100        110        120 
GKGFVLRLAP DDSFLAPEFK IERLGGSGRA TGGERGLRGC FFSGTVNGEP ESLAAVSLCR 

       130        140        150        160        170        180 
GLSGSFLLDG EEFTIQPQGA GGSLAQPHRL QRWGPAGARP LPRGPEWEVE TGEGQRQERG 

       190        200        210        220        230        240 
DHQEDSEEES QEEEAEGASE PPPPLGATSR TKRFVSEARF VETLLVADAS MAAFYGADLQ 

       250        260        270        280        290        300 
NHILTLMSVA ARIYKHPSIK NSINLMVVKV LIVEDEKWGP EVSDNGGLTL RNFCNWQRRF 

       310        320        330        340        350        360 
NQPSDRHPEH YDTAILLTRQ NFCGQEGLCD TLGVADIGTI CDPNKSCSVI EDEGLQAAHT 

       370        380        390        400        410        420 
LAHELGHVLS MPHDDSKPCT RLFGPMGKHH VMAPLFVHLN QTLPWSPCSA MYLTELLDGG 

       430        440        450        460        470        480 
HGDCLLDAPA AALPLPTGLP GRMALYQLDQ QCRQIFGPDF RHCPNTSAQD VCAQLWCHTD 

       490        500        510        520        530        540 
GAEPLCHTKN GSLPWADGTP CGPGHLCSEG SCLPEEEVER PKPVADGGWA PWGPWGECSR 

       550        560        570        580        590        600 
TCGGGVQFSH RECKDPEPQN GGRYCLGRRA KYQSCHTEEC PPDGKSFREQ QCEKYNAYNY 

       610        620        630        640        650        660 
TDMDGNLLQW VPKYAGVSPR DRCKLFCRAR GRSEFKVFEA KVIDGTLCGP ETLAICVRGQ 

       670        680        690        700        710        720 
CVKAGCDHVV DSPRKLDKCG VCGGKGNSCR KVSGSLTPTN YGYNDIVTIP AGATNIDVKQ 

       730        740        750        760        770        780 
RSHPGVQNDG NYLALKTADG QYLLNGNLAI SAIEQDILVK GTILKYSGSI ATLERLQSFR 

       790        800        810        820        830        840 
PLPEPLTVQL LTVPGEVFPP KVKYTFFVPN DVDFSMQSSK ERATTNIIQP LLHAQWVLGD 

       850        860        870        880 
WSECSSTCGA GWQRRTVECR DPSGQASATC NKALKPEDAK PCESQLCPL 

« Hide

References

« Hide 'large scale' references
[1]"METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity."
Vazquez F., Hastings G., Ortega M.-A., Lane T.F., Oikemus S., Lombardo M., Iruela-Arispe M.L.
J. Biol. Chem. 274:23349-23357(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11."
Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S.
Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-439.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF060153 mRNA. Translation: AAD48081.1.
AP002986 Genomic DNA. No translation available.
AF175283 mRNA. Translation: AAF25806.1.
CCDSCCDS41732.1.
RefSeqNP_008968.4. NM_007037.4.
XP_006718821.1. XM_006718758.1.
UniGeneHs.271605.

3D structure databases

ProteinModelPortalQ9UP79.
SMRQ9UP79. Positions 219-809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000257359.

Protein family/group databases

MEROPSM12.226.

PTM databases

PhosphoSiteQ9UP79.

Polymorphism databases

DMDM313104077.

Proteomic databases

PaxDbQ9UP79.
PRIDEQ9UP79.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000257359; ENSP00000257359; ENSG00000134917.
GeneID11095.
KEGGhsa:11095.
UCSCuc001qgg.4. human.

Organism-specific databases

CTD11095.
GeneCardsGC11M130308.
H-InvDBHIX0026131.
HGNCHGNC:224. ADAMTS8.
MIM605175. gene.
neXtProtNX_Q9UP79.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG285519.
HOGENOMHOG000004799.
HOVERGENHBG004313.
InParanoidQ9UP79.
KOK08623.
OMAYELDQQC.
OrthoDBEOG7WDN1M.
PhylomeDBQ9UP79.
TreeFamTF331949.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.
REACT_17015. Metabolism of proteins.
REACT_223425. Extracellular matrix organization.

Gene expression databases

BgeeQ9UP79.
CleanExHS_ADAMTS8.
GenevestigatorQ9UP79.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
InterProIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSPR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTSM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMSSF82895. SSF82895. 2 hits.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiADAMTS8.
GenomeRNAi11095.
NextBio42178.
PROQ9UP79.
SOURCESearch...

Entry information

Entry nameATS8_HUMAN
AccessionPrimary (citable) accession number: Q9UP79
Secondary accession number(s): Q9NZS0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 30, 2010
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM