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Q9UP65 (PA24C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic phospholipase A2 gamma

Short name=cPLA2-gamma
EC=3.1.1.4
Alternative name(s):
Phospholipase A2 group IVC
Gene names
Name:PLA2G4C
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a preference for arachidonic acid at the sn-2 position of phosphatidylcholine as compared with palmitic acid. Ref.2

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation

Not regulated by calcium.

Subcellular location

Membrane; Lipid-anchor Ref.2.

Tissue specificity

Highly expressed in heart and skeletal muscle. Ref.1 Ref.2

Sequence similarities

Contains 1 PLA2c domain.

Biophysicochemical properties

Kinetic parameters:

Vmax=218 pmol/min/mg enzyme Ref.2

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   Molecular functionHydrolase
   PTMLipoprotein
Methylation
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarachidonic acid metabolic process

Non-traceable author statement Ref.2. Source: UniProtKB

glycerophospholipid biosynthetic process

Traceable author statement. Source: Reactome

glycerophospholipid catabolic process

Inferred from direct assay Ref.2. Source: UniProtKB

inflammatory response

Non-traceable author statement Ref.2. Source: UniProtKB

intracellular signal transduction

Non-traceable author statement Ref.2. Source: UniProtKB

parturition

Non-traceable author statement Ref.2. Source: UniProtKB

phosphatidylcholine acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylethanolamine acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylinositol acyl-chain remodeling

Traceable author statement. Source: Reactome

phospholipid metabolic process

Traceable author statement Ref.2. Source: UniProtKB

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentcell cortex

Inferred from electronic annotation. Source: Ensembl

cytosol

Traceable author statement Ref.2. Source: UniProtKB

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

membrane

Traceable author statement Ref.2. Source: UniProtKB

nuclear envelope

Inferred from electronic annotation. Source: Ensembl

nucleoplasm

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncalcium-independent phospholipase A2 activity

Inferred from direct assay Ref.2. Source: UniProtKB

phospholipid binding

Non-traceable author statement Ref.2. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UP65-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UP65-2)

The sequence of this isoform differs from the canonical sequence as follows:
     528-541: Missing.
Isoform 3 (identifier: Q9UP65-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MGSSEVSIIPGLQ → MRTRPRPRLRRTENFLTAVHHGK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 538538Cytosolic phospholipase A2 gamma
PRO_0000022995
Propeptide539 – 5413Removed in mature form
PRO_0000022996

Regions

Domain1 – 541541PLA2c

Sites

Active site821Nucleophile By similarity
Active site3851Proton acceptor By similarity

Amino acid modifications

Modified residue5381Cysteine methyl ester Ref.11
Lipidation5381S-farnesyl cysteine Ref.11

Natural variations

Alternative sequence1 – 1313MGSSE…IPGLQ → MRTRPRPRLRRTENFLTAVH HGK in isoform 3.
VSP_045849
Alternative sequence528 – 54114Missing in isoform 2.
VSP_045850
Natural variant211E → K. Ref.3
Corresponds to variant rs11564522 [ dbSNP | Ensembl ].
VAR_018761
Natural variant381A → P. Ref.3
Corresponds to variant rs2307279 [ dbSNP | Ensembl ].
VAR_018420
Natural variant1271A → V. Ref.3
Corresponds to variant rs11564532 [ dbSNP | Ensembl ].
VAR_018762
Natural variant1421V → F. Ref.3
Corresponds to variant rs11564534 [ dbSNP | Ensembl ].
VAR_018763
Natural variant1431I → V. Ref.3 Ref.4
Corresponds to variant rs2303744 [ dbSNP | Ensembl ].
VAR_018421
Natural variant1481R → G. Ref.3
Corresponds to variant rs2307282 [ dbSNP | Ensembl ].
VAR_018764
Natural variant1511P → L. Ref.3
Corresponds to variant rs11564538 [ dbSNP | Ensembl ].
VAR_018765
Natural variant2031S → P. Ref.2 Ref.3 Ref.4 Ref.5
Corresponds to variant rs156631 [ dbSNP | Ensembl ].
VAR_018422
Natural variant2261T → S. Ref.3
Corresponds to variant rs11564541 [ dbSNP | Ensembl ].
VAR_018766
Natural variant3601T → P. Ref.3
Corresponds to variant rs11564620 [ dbSNP | Ensembl ].
VAR_018767
Natural variant4111D → N. Ref.3
Corresponds to variant rs11564638 [ dbSNP | Ensembl ].
VAR_018768
Natural variant4301R → C. Ref.9
Corresponds to variant rs191276960 [ dbSNP | Ensembl ].
VAR_018423

Experimental info

Mutagenesis541R → A: Abolishes enzyme activity. Ref.2
Mutagenesis821S → A: Abolishes enzyme activity. Ref.2
Mutagenesis3851D → A: Abolishes enzyme activity. Ref.2
Mutagenesis4021R → A: Abolishes enzyme activity. Ref.2
Sequence conflict241R → G in BAG58352. Ref.4
Sequence conflict4651M → I in AAC32823. Ref.1
Sequence conflict4801E → G in CAG33097. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 8, 2011. Version 2.
Checksum: A114B2A614EA4632

FASTA54160,939
        10         20         30         40         50         60 
MGSSEVSIIP GLQKEEKAAV ERRRLHVLKA LKKLRIEADE APVVAVLGSG GGLRAHIACL 

        70         80         90        100        110        120 
GVLSEMKEQG LLDAVTYLAG VSGSTWAISS LYTNDGDMEA LEADLKHRFT RQEWDLAKSL 

       130        140        150        160        170        180 
QKTIQAARSE NYSLTDFWAY MVISKQTREL PESHLSNMKK PVEEGTLPYP IFAAIDNDLQ 

       190        200        210        220        230        240 
PSWQEARAPE TWFEFTPHHA GFSALGAFVS ITHFGSKFKK GRLVRTHPER DLTFLRGLWG 

       250        260        270        280        290        300 
SALGNTEVIR EYIFDQLRNL TLKGLWRRAV ANAKSIGHLI FARLLRLQES SQGEHPPPED 

       310        320        330        340        350        360 
EGGEPEHTWL TEMLENWTRT SLEKQEQPHE DPERKGSLSN LMDFVKKTGI CASKWEWGTT 

       370        380        390        400        410        420 
HNFLYKHGGI RDKIMSSRKH LHLVDAGLAI NTPFPLVLPP TREVHLILSF DFSAGDPFET 

       430        440        450        460        470        480 
IRATTDYCRR HKIPFPQVEE AELDLWSKAP ASCYILKGET GPVVMHFPLF NIDACGGDIE 

       490        500        510        520        530        540 
AWSDTYDTFK LADTYTLDVV VLLLALAKKN VRENKKKILR ELMNVAGLYY PKDSARSCCL 


A 

« Hide

Isoform 2 [UniParc].

Checksum: 4F4C4884DE7EAD3A
Show »

FASTA52759,367
Isoform 3 [UniParc].

Checksum: D9A68B760776F943
Show »

FASTA55162,451

References

« Hide 'large scale' references
[1]"A novel calcium-independent phospholipase A2, cPLA2-gamma, that is prenylated and contains homology to cPLA2."
Underwood K.W., Song C., Kriz R.W., Chang X.J., Knopf J.L., Lin L.L.
J. Biol. Chem. 273:21926-21932(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, TISSUE SPECIFICITY.
Tissue: Skeletal muscle.
[2]"Molecular cloning of two new human paralogs of 85-kDa cytosolic phospholipase A2."
Pickard R.T., Strifler B.A., Kramer R.M., Sharp J.D.
J. Biol. Chem. 274:8823-8831(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-54; SER-82; ASP-385 AND ARG-402, VARIANT PRO-203.
[3]NIEHS SNPs program
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-21; PRO-38; VAL-127; PHE-142; VAL-143; GLY-148; LEU-151; PRO-203; SER-226; PRO-360 AND ASN-411.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANTS VAL-143 AND PRO-203.
Tissue: Corpus callosum and Tongue.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-203.
[6]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pancreas.
[9]"SNP search of PLA2G4C gene."
Koyama K.S., Kimura T., Imai T.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 420-476, VARIANT CYS-430.
[10]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 443-541 (ISOFORM 1).
Tissue: Uterus.
[11]"Purification of recombinant human cPLA2 gamma and identification of C-terminal farnesylation, proteolytic processing, and carboxymethylation by MALDI-TOF-TOF analysis."
Jenkins C.M., Han X., Yang J., Mancuso D.J., Sims H.F., Muslin A.J., Gross R.W.
Biochemistry 42:11798-11807(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-538, METHYLATION AT CYS-538.
[12]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF058921 mRNA. Translation: AAC32823.1.
AF065214 mRNA. Translation: AAC78835.1.
AY485310 Genomic DNA. Translation: AAR25453.1.
AK295400 mRNA. Translation: BAG58352.1.
AK314524 mRNA. Translation: BAG37118.1.
CR456816 mRNA. Translation: CAG33097.1.
AC010458 Genomic DNA. No translation available.
AC011466 Genomic DNA. No translation available.
CH471177 Genomic DNA. Translation: EAW52314.1.
BC063416 mRNA. Translation: AAH63416.1.
AB105807 Genomic DNA. Translation: BAC87745.1.
AL050193 mRNA. Translation: CAB43312.3.
PIRT13162.
RefSeqNP_001152794.1. NM_001159322.1.
NP_001152795.1. NM_001159323.1.
NP_003697.2. NM_003706.2.
UniGeneHs.631562.

3D structure databases

ProteinModelPortalQ9UP65.
SMRQ9UP65. Positions 3-527.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114165. 2 interactions.
STRING9606.ENSP00000346228.

Chemistry

BindingDBQ9UP65.
ChEMBLCHEMBL4834.

PTM databases

PhosphoSiteQ9UP65.

Polymorphism databases

DMDM322510066.

Proteomic databases

PaxDbQ9UP65.
PRIDEQ9UP65.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354276; ENSP00000346228; ENSG00000105499. [Q9UP65-2]
ENST00000413144; ENSP00000400036; ENSG00000105499. [Q9UP65-1]
ENST00000599111; ENSP00000472546; ENSG00000105499. [Q9UP65-3]
ENST00000599921; ENSP00000469473; ENSG00000105499. [Q9UP65-1]
GeneID8605.
KEGGhsa:8605.
UCSCuc002phw.3. human. [Q9UP65-1]

Organism-specific databases

CTD8605.
GeneCardsGC19M048551.
HGNCHGNC:9037. PLA2G4C.
HPAHPA043083.
MIM603602. gene.
neXtProtNX_Q9UP65.
PharmGKBPA33365.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG278787.
HOVERGENHBG058910.
InParanoidQ9UP65.
KOK16342.
OMAKDSARSC.
OrthoDBEOG78SQJS.
PhylomeDBQ9UP65.
TreeFamTF325228.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressQ9UP65.
BgeeQ9UP65.
CleanExHS_PLA2G4C.
GenevestigatorQ9UP65.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 1 hit.
PROSITEPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPLA2G4C.
GenomeRNAi8605.
NextBio32245.
PROQ9UP65.
SOURCESearch...

Entry information

Entry namePA24C_HUMAN
AccessionPrimary (citable) accession number: Q9UP65
Secondary accession number(s): B2RB71 expand/collapse secondary AC list , B4DI40, O75457, Q6IBI8, Q9UG68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM