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Protein

NSFL1 cofactor p47

Gene

NSFL1C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

SIGNORiQ9UNZ2.

Names & Taxonomyi

Protein namesi
Recommended name:
NSFL1 cofactor p47
Alternative name(s):
UBX domain-containing protein 2C
p97 cofactor p47
Gene namesi
Name:NSFL1C
Synonyms:UBXN2C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15912. NSFL1C.

Subcellular locationi

  • Nucleus By similarity
  • Golgi apparatusGolgi stack By similarity
  • Chromosome By similarity

  • Note: Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Golgi apparatus, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55968.
OpenTargetsiENSG00000088833.
PharmGKBiPA31794.

Polymorphism and mutation databases

BioMutaiNSFL1C.
DMDMi41017512.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109881 – 370NSFL1 cofactor p47Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei167PhosphotyrosineBy similarity1
Modified residuei176PhosphoserineBy similarity1
Modified residuei192PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UNZ2.
PaxDbiQ9UNZ2.
PeptideAtlasiQ9UNZ2.
PRIDEiQ9UNZ2.
TopDownProteomicsiQ9UNZ2-1. [Q9UNZ2-1]
Q9UNZ2-4. [Q9UNZ2-4]
Q9UNZ2-5. [Q9UNZ2-5]

2D gel databases

REPRODUCTION-2DPAGEIPI00100197.

PTM databases

iPTMnetiQ9UNZ2.
PhosphoSitePlusiQ9UNZ2.

Expressioni

Gene expression databases

BgeeiENSG00000088833.
CleanExiHS_NSFL1C.
ExpressionAtlasiQ9UNZ2. baseline and differential.
GenevisibleiQ9UNZ2. HS.

Organism-specific databases

HPAiHPA047108.
HPA050628.

Interactioni

Subunit structurei

Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HEL-S-70V9HW803EBI-721577,EBI-10175326
VCPP550725EBI-721577,EBI-355164

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121014. 81 interactors.
DIPiDIP-39611N.
IntActiQ9UNZ2. 25 interactors.
MINTiMINT-3083194.
STRINGi9606.ENSP00000418529.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi171 – 173Combined sources3
Beta strandi180 – 193Combined sources14
Beta strandi198 – 200Combined sources3
Turni204 – 206Combined sources3
Helixi207 – 215Combined sources9
Helixi220 – 223Combined sources4
Beta strandi231 – 239Combined sources9
Beta strandi245 – 250Combined sources6
Beta strandi255 – 259Combined sources5
Helixi260 – 262Combined sources3
Beta strandi265 – 268Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SS6NMR-A171-270[»]
ProteinModelPortaliQ9UNZ2.
SMRiQ9UNZ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNZ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 244SEPPROSITE-ProRule annotationAdd BLAST66
Domaini291 – 368UBXPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 115Nuclear localization signal7
Motifi172 – 175Nuclear localization signal4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi84 – 90Poly-Glu7

Sequence similaritiesi

Belongs to the NSFL1C family.Curated
Contains 1 SEP domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2086. Eukaryota.
ENOG410YGXU. LUCA.
GeneTreeiENSGT00520000055567.
HOVERGENiHBG054517.
InParanoidiQ9UNZ2.
KOiK14012.
OMAiQTIGDVY.
OrthoDBiEOG091G0QFZ.
PhylomeDBiQ9UNZ2.
TreeFamiTF312973.

Family and domain databases

InterProiIPR012989. SEP_domain.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF102848. SSF102848. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNZ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI
60 70 80 90 100
VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRFYAGGSE
110 120 130 140 150
RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF
160 170 180 190 200
AGGGYRLGAA PEEESAYVAG EKRQHSSQDV HVVLKLWKSG FSLDNGELRS
210 220 230 240 250
YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF
260 270 280 290 300
KAFTGEGQKL GSTAPQVLST SSPAQQAENE AKASSSILID ESEPTTNIQI
310 320 330 340 350
RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFILM TTFPNKELAD
360 370
ESQTLKEANL LNAVIVQRLT
Length:370
Mass (Da):40,573
Last modified:January 16, 2004 - v2
Checksum:i79364617F940B9F9
GO
Isoform 2 (identifier: Q9UNZ2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-179: Missing.

Note: No experimental confirmation available.
Show »
Length:339
Mass (Da):37,325
Checksum:iCF165EC48BB54B96
GO
Isoform 3 (identifier: Q9UNZ2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-93: R → RSR

Show »
Length:372
Mass (Da):40,816
Checksum:i4EFCFE27D995031F
GO
Isoform 4 (identifier: Q9UNZ2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: MAAERQEALR...PNELVDDLFK → MTKMKMRRKRKAR

Show »
Length:259
Mass (Da):28,522
Checksum:i5400E8D45F25448A
GO

Sequence cautioni

The sequence AAF17199 differs from that shown. Sequencing errors.Curated
The sequence AAF17199 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39 – 40AS → EL in AAP97139 (Ref. 1) Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017481290D → N.1 PublicationCorresponds to variant rs9575dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0410621 – 124MAAER…DDLFK → MTKMKMRRKRKAR in isoform 4. 1 PublicationAdd BLAST124
Alternative sequenceiVSP_00926293R → RSR in isoform 3. 1 Publication1
Alternative sequenceiVSP_009263149 – 179Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086909 mRNA. Translation: AAP97139.1.
AF112211 mRNA. Translation: AAF17199.1. Sequence problems.
AF078856 mRNA. Translation: AAD44488.1.
AK297403 mRNA. Translation: BAG59842.1.
AK001511 mRNA. Translation: BAA91731.1.
AK315433 mRNA. Translation: BAG37821.1.
AL109658 Genomic DNA. Translation: CAB96827.1.
AL109658 Genomic DNA. Translation: CAI22730.1.
AL109658 Genomic DNA. Translation: CAI22731.1.
AL109658 Genomic DNA. Translation: CAM28306.1.
CH471133 Genomic DNA. Translation: EAX10629.1.
CH471133 Genomic DNA. Translation: EAX10631.1.
BC002801 mRNA. Translation: AAH02801.1.
CCDSiCCDS13015.1. [Q9UNZ2-1]
CCDS13016.1. [Q9UNZ2-4]
CCDS56175.1. [Q9UNZ2-5]
RefSeqiNP_001193665.1. NM_001206736.1. [Q9UNZ2-5]
NP_057227.2. NM_016143.4. [Q9UNZ2-1]
NP_061327.2. NM_018839.4. [Q9UNZ2-4]
XP_006723657.1. XM_006723594.2. [Q9UNZ2-6]
UniGeneiHs.12865.

Genome annotation databases

EnsembliENST00000216879; ENSP00000216879; ENSG00000088833. [Q9UNZ2-1]
ENST00000353088; ENSP00000338643; ENSG00000088833. [Q9UNZ2-4]
ENST00000476071; ENSP00000418529; ENSG00000088833. [Q9UNZ2-5]
GeneIDi55968.
KEGGihsa:55968.
UCSCiuc002wfc.3. human. [Q9UNZ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086909 mRNA. Translation: AAP97139.1.
AF112211 mRNA. Translation: AAF17199.1. Sequence problems.
AF078856 mRNA. Translation: AAD44488.1.
AK297403 mRNA. Translation: BAG59842.1.
AK001511 mRNA. Translation: BAA91731.1.
AK315433 mRNA. Translation: BAG37821.1.
AL109658 Genomic DNA. Translation: CAB96827.1.
AL109658 Genomic DNA. Translation: CAI22730.1.
AL109658 Genomic DNA. Translation: CAI22731.1.
AL109658 Genomic DNA. Translation: CAM28306.1.
CH471133 Genomic DNA. Translation: EAX10629.1.
CH471133 Genomic DNA. Translation: EAX10631.1.
BC002801 mRNA. Translation: AAH02801.1.
CCDSiCCDS13015.1. [Q9UNZ2-1]
CCDS13016.1. [Q9UNZ2-4]
CCDS56175.1. [Q9UNZ2-5]
RefSeqiNP_001193665.1. NM_001206736.1. [Q9UNZ2-5]
NP_057227.2. NM_016143.4. [Q9UNZ2-1]
NP_061327.2. NM_018839.4. [Q9UNZ2-4]
XP_006723657.1. XM_006723594.2. [Q9UNZ2-6]
UniGeneiHs.12865.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SS6NMR-A171-270[»]
ProteinModelPortaliQ9UNZ2.
SMRiQ9UNZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121014. 81 interactors.
DIPiDIP-39611N.
IntActiQ9UNZ2. 25 interactors.
MINTiMINT-3083194.
STRINGi9606.ENSP00000418529.

PTM databases

iPTMnetiQ9UNZ2.
PhosphoSitePlusiQ9UNZ2.

Polymorphism and mutation databases

BioMutaiNSFL1C.
DMDMi41017512.

2D gel databases

REPRODUCTION-2DPAGEIPI00100197.

Proteomic databases

EPDiQ9UNZ2.
PaxDbiQ9UNZ2.
PeptideAtlasiQ9UNZ2.
PRIDEiQ9UNZ2.
TopDownProteomicsiQ9UNZ2-1. [Q9UNZ2-1]
Q9UNZ2-4. [Q9UNZ2-4]
Q9UNZ2-5. [Q9UNZ2-5]

Protocols and materials databases

DNASUi55968.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216879; ENSP00000216879; ENSG00000088833. [Q9UNZ2-1]
ENST00000353088; ENSP00000338643; ENSG00000088833. [Q9UNZ2-4]
ENST00000476071; ENSP00000418529; ENSG00000088833. [Q9UNZ2-5]
GeneIDi55968.
KEGGihsa:55968.
UCSCiuc002wfc.3. human. [Q9UNZ2-1]

Organism-specific databases

CTDi55968.
DisGeNETi55968.
GeneCardsiNSFL1C.
HGNCiHGNC:15912. NSFL1C.
HPAiHPA047108.
HPA050628.
MIMi606610. gene.
neXtProtiNX_Q9UNZ2.
OpenTargetsiENSG00000088833.
PharmGKBiPA31794.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2086. Eukaryota.
ENOG410YGXU. LUCA.
GeneTreeiENSGT00520000055567.
HOVERGENiHBG054517.
InParanoidiQ9UNZ2.
KOiK14012.
OMAiQTIGDVY.
OrthoDBiEOG091G0QFZ.
PhylomeDBiQ9UNZ2.
TreeFamiTF312973.

Enzyme and pathway databases

SIGNORiQ9UNZ2.

Miscellaneous databases

ChiTaRSiNSFL1C. human.
EvolutionaryTraceiQ9UNZ2.
GeneWikiiNSFL1C.
GenomeRNAii55968.
PROiQ9UNZ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088833.
CleanExiHS_NSFL1C.
ExpressionAtlasiQ9UNZ2. baseline and differential.
GenevisibleiQ9UNZ2. HS.

Family and domain databases

InterProiIPR012989. SEP_domain.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF102848. SSF102848. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSF1C_HUMAN
AccessioniPrimary (citable) accession number: Q9UNZ2
Secondary accession number(s): A2A2L1
, B2RD74, Q5JXA4, Q5JXA5, Q7Z533, Q9H102, Q9NVL9, Q9UI06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.