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Protein

Transcription termination factor 2

Gene

TTF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi596 – 603ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: ProtInc
  • zinc ion binding Source: InterPro

GO - Biological processi

  • DNA-templated transcription, termination Source: ProtInc
  • mRNA processing Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA splicing Source: UniProtKB-KW
  • termination of RNA polymerase II transcription Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

SIGNORiQ9UNY4.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor 2 (EC:3.6.4.-)
Alternative name(s):
Lodestar homolog
RNA polymerase II termination factor
Transcription release factor 2
Short name:
F2
Short name:
HuF2
Gene namesi
Name:TTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:12398. TTF2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.

GO - Cellular componenti

  • cytoplasm Source: HPA
  • spliceosomal complex Source: UniProtKB-KW
  • transcription elongation factor complex Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8458.
MalaCardsiTTF2.
OpenTargetsiENSG00000116830.
PharmGKBiPA37063.

Polymorphism and mutation databases

BioMutaiTTF2.
DMDMi73920148.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743761 – 1162Transcription termination factor 2Add BLAST1162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei460PhosphoserineCombined sources1
Modified residuei883PhosphoserineCombined sources1
Modified residuei908PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UNY4.
PaxDbiQ9UNY4.
PeptideAtlasiQ9UNY4.
PRIDEiQ9UNY4.

PTM databases

iPTMnetiQ9UNY4.
PhosphoSitePlusiQ9UNY4.

Expressioni

Gene expression databases

BgeeiENSG00000116830.
CleanExiHS_TTF2.
ExpressionAtlasiQ9UNY4. baseline and differential.
GenevisibleiQ9UNY4. HS.

Organism-specific databases

HPAiHPA005776.
HPA070762.

Interactioni

Subunit structurei

Interacts with CDC5L. Part of the spliceosome.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994595EBI-2322921,EBI-374880

Protein-protein interaction databases

BioGridi114036. 54 interactors.
IntActiQ9UNY4. 44 interactors.
MINTiMINT-272557.
STRINGi9606.ENSP00000358478.

Structurei

3D structure databases

ProteinModelPortaliQ9UNY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini583 – 786Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST204
Domaini995 – 1157Helicase C-terminalPROSITE-ProRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi737 – 740DEAH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410INU3. Eukaryota.
ENOG410XPDU. LUCA.
GeneTreeiENSGT00860000133848.
HOGENOMiHOG000154708.
HOVERGENiHBG053179.
InParanoidiQ9UNY4.
KOiK15173.
OMAiWCGSIPW.
OrthoDBiEOG091G08AX.
PhylomeDBiQ9UNY4.
TreeFamiTF316297.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR010666. Znf_GRF.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNY4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEVRCPEHG TFCFLKTGVR DGPNKGKSFY VCRADTCSFV RATDIPVSHC
60 70 80 90 100
LLHEDFVVEL QGLLLPQDKK EYRLFFRCIR SKAEGKRWCG SIPWQDPDSK
110 120 130 140 150
EHSVSNKSQH ASETFHHSSN WLRNPFKVLD KNQEPALWKQ LIKGEGEEKK
160 170 180 190 200
ADKKQREKGD QLFDQKKEQK PEMMEKDLSS GLVPKKKQSV VQEKKQEEGA
210 220 230 240 250
EIQCEAETGG THKRDFSEIK SQQCQGNELT RPSASSQEKS SGKSQDVQRE
260 270 280 290 300
SEPLREKVTQ LLPQNVHSHN SISKPQKGGP LNKEYTNWEA KETKAKDGPS
310 320 330 340 350
IQATQKSLPQ GHFQERPETH SVPAPGGPAA QAAPAAPGLS LGEGREAATS
360 370 380 390 400
SDDEEEDDVV FVSSKPGSPL LFDSTLDLET KENLQFPDRS VQRKVSPASG
410 420 430 440 450
VSKKVEPSDP VARRVYLTTQ LKQKKSTLAS VNIQALPDKG QKLIKQIQEL
460 470 480 490 500
EEVLSGLTLS PEQGTNEKSN SQVPQQSHFT KTTTGPPHLV PPQPLPRRGT
510 520 530 540 550
QPVGSLELKS ACQVTAGGSS QCYRGHTNQD HVHAVWKITS EAIGQLHRSL
560 570 580 590 600
ESCPGETVVA EDPAGLKVPL LLHQKQALAW LLWRESQKPQ GGILADDMGL
610 620 630 640 650
GKTLTMIALI LTQKNQEKKE EKEKSTALTW LSKDDSCDFT SHGTLIICPA
660 670 680 690 700
SLIHHWKNEV EKRVNSNKLR VYLYHGPNRD SRARVLSTYD IVITTYSLVA
710 720 730 740 750
KEIPTNKQEA EIPGANLNVE GTSTPLLRIA WARIILDEAH NVKNPRVQTS
760 770 780 790 800
IAVCKLQACA RWAVTGTPIQ NNLLDMYSLL KFLRCSPFDE FNLWRSQVDN
810 820 830 840 850
GSKKGGERLS ILTKSLLLRR TKDQLDSTGR PLVILPQRKF QLHHLKLSED
860 870 880 890 900
EETVYNVFFA RSRSALQSYL KRHESRGNQS GRSPNNPFSR VALEFGSEEP
910 920 930 940 950
RHSEAADSPR SSTVHILSQL LRLRQCCCHL SLLKSALDPM ELKGEGLVLS
960 970 980 990 1000
LEEQLSALTL SELRDSEPSS TVSLNGTFFK MELFEGMRES TKISSLLAEL
1010 1020 1030 1040 1050
EAIQRNSASQ KSVIVSQWTN MLKVVALHLK KHGLTYATID GSVNPKQRMD
1060 1070 1080 1090 1100
LVEAFNHSRG PQVMLISLLA GGVGLNLTGG NHLFLLDMHW NPSLEDQACD
1110 1120 1130 1140 1150
RIYRVGQQKD VVIHRFVCEG TVEEKILQLQ EKKKDLAKQV LSGSGESVTK
1160
LTLADLRVLF GI
Length:1,162
Mass (Da):129,588
Last modified:August 30, 2005 - v2
Checksum:i858BAA22B2D2D5C9
GO
Isoform 2 (identifier: Q9UNY4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     635-676: DSCDFTSHGT...NKLRVYLYHG → GRQKCLNSLP...YLITLLENQY
     677-1162: Missing.

Note: No experimental confirmation available.
Show »
Length:676
Mass (Da):75,128
Checksum:i9CB5D331F1848C97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64L → P in AAD49435 (PubMed:12927788).Curated1
Sequence conflicti80R → G in AAD49435 (PubMed:12927788).Curated1
Sequence conflicti675H → R in AAC64044 (PubMed:9748214).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023393167K → E.1 PublicationCorresponds to variant rs998532dbSNPEnsembl.1
Natural variantiVAR_034431213K → R.Corresponds to variant rs7535524dbSNPEnsembl.1
Natural variantiVAR_061234256E → G.Corresponds to variant rs34334470dbSNPEnsembl.1
Natural variantiVAR_0612351134K → R.Corresponds to variant rs41276572dbSNPEnsembl.1
Natural variantiVAR_0612361155D → H.1 PublicationCorresponds to variant rs34236116dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015370635 – 676DSCDF…YLYHG → GRQKCLNSLPFPTSFEPPKR GTSSAKKGHLWSYLITLLEN QY in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_015371677 – 1162Missing in isoform 2. 1 PublicationAdd BLAST486

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073771 mRNA. Translation: AAC64044.1.
AF080255 mRNA. Translation: AAD49435.1.
AK291017 mRNA. Translation: BAF83706.1.
AL445231, AL391476 Genomic DNA. Translation: CAH71961.1.
AL391476, AL445231 Genomic DNA. Translation: CAI12738.1.
AL391476 Genomic DNA. Translation: CAI12731.1.
BC030058 mRNA. Translation: AAH30058.1.
CCDSiCCDS892.1. [Q9UNY4-1]
RefSeqiNP_003585.3. NM_003594.3. [Q9UNY4-1]
XP_016858044.1. XM_017002555.1. [Q9UNY4-1]
UniGeneiHs.32333.
Hs.486818.

Genome annotation databases

EnsembliENST00000369466; ENSP00000358478; ENSG00000116830. [Q9UNY4-1]
GeneIDi8458.
KEGGihsa:8458.
UCSCiuc001egy.4. human. [Q9UNY4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073771 mRNA. Translation: AAC64044.1.
AF080255 mRNA. Translation: AAD49435.1.
AK291017 mRNA. Translation: BAF83706.1.
AL445231, AL391476 Genomic DNA. Translation: CAH71961.1.
AL391476, AL445231 Genomic DNA. Translation: CAI12738.1.
AL391476 Genomic DNA. Translation: CAI12731.1.
BC030058 mRNA. Translation: AAH30058.1.
CCDSiCCDS892.1. [Q9UNY4-1]
RefSeqiNP_003585.3. NM_003594.3. [Q9UNY4-1]
XP_016858044.1. XM_017002555.1. [Q9UNY4-1]
UniGeneiHs.32333.
Hs.486818.

3D structure databases

ProteinModelPortaliQ9UNY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114036. 54 interactors.
IntActiQ9UNY4. 44 interactors.
MINTiMINT-272557.
STRINGi9606.ENSP00000358478.

PTM databases

iPTMnetiQ9UNY4.
PhosphoSitePlusiQ9UNY4.

Polymorphism and mutation databases

BioMutaiTTF2.
DMDMi73920148.

Proteomic databases

EPDiQ9UNY4.
PaxDbiQ9UNY4.
PeptideAtlasiQ9UNY4.
PRIDEiQ9UNY4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369466; ENSP00000358478; ENSG00000116830. [Q9UNY4-1]
GeneIDi8458.
KEGGihsa:8458.
UCSCiuc001egy.4. human. [Q9UNY4-1]

Organism-specific databases

CTDi8458.
DisGeNETi8458.
GeneCardsiTTF2.
H-InvDBHIX0000933.
HGNCiHGNC:12398. TTF2.
HPAiHPA005776.
HPA070762.
MalaCardsiTTF2.
MIMi604718. gene.
neXtProtiNX_Q9UNY4.
OpenTargetsiENSG00000116830.
PharmGKBiPA37063.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INU3. Eukaryota.
ENOG410XPDU. LUCA.
GeneTreeiENSGT00860000133848.
HOGENOMiHOG000154708.
HOVERGENiHBG053179.
InParanoidiQ9UNY4.
KOiK15173.
OMAiWCGSIPW.
OrthoDBiEOG091G08AX.
PhylomeDBiQ9UNY4.
TreeFamiTF316297.

Enzyme and pathway databases

SIGNORiQ9UNY4.

Miscellaneous databases

ChiTaRSiTTF2. human.
GeneWikiiTTF2.
GenomeRNAii8458.
PROiQ9UNY4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116830.
CleanExiHS_TTF2.
ExpressionAtlasiQ9UNY4. baseline and differential.
GenevisibleiQ9UNY4. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR010666. Znf_GRF.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTTF2_HUMAN
AccessioniPrimary (citable) accession number: Q9UNY4
Secondary accession number(s): A8K4Q2
, O75921, Q5T2K7, Q5VVU8, Q8N6I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.