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Protein

Transcription termination factor 2

Gene

TTF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi596 – 603ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: ProtInc
  • helicase activity Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

  • DNA-templated transcription, termination Source: ProtInc
  • mRNA processing Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA splicing Source: UniProtKB-KW
  • termination of RNA polymerase II transcription Source: ProtInc

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processmRNA processing, mRNA splicing, Transcription, Transcription regulation, Transcription termination
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SIGNORiQ9UNY4

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor 2 (EC:3.6.4.-)
Alternative name(s):
Lodestar homolog
RNA polymerase II termination factor
Transcription release factor 2
Short name:
F2
Short name:
HuF2
Gene namesi
Name:TTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116830.11
HGNCiHGNC:12398 TTF2
MIMi604718 gene
neXtProtiNX_Q9UNY4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8458
OpenTargetsiENSG00000116830
PharmGKBiPA37063

Polymorphism and mutation databases

BioMutaiTTF2
DMDMi73920148

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743761 – 1162Transcription termination factor 2Add BLAST1162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki143Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei460PhosphoserineCombined sources1
Modified residuei883PhosphoserineCombined sources1
Modified residuei908PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UNY4
PaxDbiQ9UNY4
PeptideAtlasiQ9UNY4
PRIDEiQ9UNY4

PTM databases

iPTMnetiQ9UNY4
PhosphoSitePlusiQ9UNY4

Expressioni

Gene expression databases

BgeeiENSG00000116830
CleanExiHS_TTF2
ExpressionAtlasiQ9UNY4 baseline and differential
GenevisibleiQ9UNY4 HS

Organism-specific databases

HPAiHPA005776
HPA070762

Interactioni

Subunit structurei

Interacts with CDC5L. Part of the spliceosome.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994595EBI-2322921,EBI-374880

Protein-protein interaction databases

BioGridi114036, 58 interactors
IntActiQ9UNY4, 44 interactors
MINTiQ9UNY4
STRINGi9606.ENSP00000358478

Structurei

3D structure databases

ProteinModelPortaliQ9UNY4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini583 – 786Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST204
Domaini995 – 1157Helicase C-terminalPROSITE-ProRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi737 – 740DEAH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Phylogenomic databases

eggNOGiENOG410INU3 Eukaryota
ENOG410XPDU LUCA
GeneTreeiENSGT00910000144263
HOGENOMiHOG000154708
HOVERGENiHBG053179
InParanoidiQ9UNY4
KOiK15173
OMAiQWTSMLK
OrthoDBiEOG091G08AX
PhylomeDBiQ9UNY4
TreeFamiTF316297

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
IPR010666 Znf_GRF
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
PF06839 zf-GRF, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNY4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEVRCPEHG TFCFLKTGVR DGPNKGKSFY VCRADTCSFV RATDIPVSHC
60 70 80 90 100
LLHEDFVVEL QGLLLPQDKK EYRLFFRCIR SKAEGKRWCG SIPWQDPDSK
110 120 130 140 150
EHSVSNKSQH ASETFHHSSN WLRNPFKVLD KNQEPALWKQ LIKGEGEEKK
160 170 180 190 200
ADKKQREKGD QLFDQKKEQK PEMMEKDLSS GLVPKKKQSV VQEKKQEEGA
210 220 230 240 250
EIQCEAETGG THKRDFSEIK SQQCQGNELT RPSASSQEKS SGKSQDVQRE
260 270 280 290 300
SEPLREKVTQ LLPQNVHSHN SISKPQKGGP LNKEYTNWEA KETKAKDGPS
310 320 330 340 350
IQATQKSLPQ GHFQERPETH SVPAPGGPAA QAAPAAPGLS LGEGREAATS
360 370 380 390 400
SDDEEEDDVV FVSSKPGSPL LFDSTLDLET KENLQFPDRS VQRKVSPASG
410 420 430 440 450
VSKKVEPSDP VARRVYLTTQ LKQKKSTLAS VNIQALPDKG QKLIKQIQEL
460 470 480 490 500
EEVLSGLTLS PEQGTNEKSN SQVPQQSHFT KTTTGPPHLV PPQPLPRRGT
510 520 530 540 550
QPVGSLELKS ACQVTAGGSS QCYRGHTNQD HVHAVWKITS EAIGQLHRSL
560 570 580 590 600
ESCPGETVVA EDPAGLKVPL LLHQKQALAW LLWRESQKPQ GGILADDMGL
610 620 630 640 650
GKTLTMIALI LTQKNQEKKE EKEKSTALTW LSKDDSCDFT SHGTLIICPA
660 670 680 690 700
SLIHHWKNEV EKRVNSNKLR VYLYHGPNRD SRARVLSTYD IVITTYSLVA
710 720 730 740 750
KEIPTNKQEA EIPGANLNVE GTSTPLLRIA WARIILDEAH NVKNPRVQTS
760 770 780 790 800
IAVCKLQACA RWAVTGTPIQ NNLLDMYSLL KFLRCSPFDE FNLWRSQVDN
810 820 830 840 850
GSKKGGERLS ILTKSLLLRR TKDQLDSTGR PLVILPQRKF QLHHLKLSED
860 870 880 890 900
EETVYNVFFA RSRSALQSYL KRHESRGNQS GRSPNNPFSR VALEFGSEEP
910 920 930 940 950
RHSEAADSPR SSTVHILSQL LRLRQCCCHL SLLKSALDPM ELKGEGLVLS
960 970 980 990 1000
LEEQLSALTL SELRDSEPSS TVSLNGTFFK MELFEGMRES TKISSLLAEL
1010 1020 1030 1040 1050
EAIQRNSASQ KSVIVSQWTN MLKVVALHLK KHGLTYATID GSVNPKQRMD
1060 1070 1080 1090 1100
LVEAFNHSRG PQVMLISLLA GGVGLNLTGG NHLFLLDMHW NPSLEDQACD
1110 1120 1130 1140 1150
RIYRVGQQKD VVIHRFVCEG TVEEKILQLQ EKKKDLAKQV LSGSGESVTK
1160
LTLADLRVLF GI
Length:1,162
Mass (Da):129,588
Last modified:August 30, 2005 - v2
Checksum:i858BAA22B2D2D5C9
GO
Isoform 2 (identifier: Q9UNY4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     635-676: DSCDFTSHGT...NKLRVYLYHG → GRQKCLNSLP...YLITLLENQY
     677-1162: Missing.

Note: No experimental confirmation available.
Show »
Length:676
Mass (Da):75,128
Checksum:i9CB5D331F1848C97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64L → P in AAD49435 (PubMed:12927788).Curated1
Sequence conflicti80R → G in AAD49435 (PubMed:12927788).Curated1
Sequence conflicti675H → R in AAC64044 (PubMed:9748214).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023393167K → E1 PublicationCorresponds to variant dbSNP:rs998532Ensembl.1
Natural variantiVAR_034431213K → R. Corresponds to variant dbSNP:rs7535524Ensembl.1
Natural variantiVAR_061234256E → G. Corresponds to variant dbSNP:rs34334470Ensembl.1
Natural variantiVAR_0612351134K → R. Corresponds to variant dbSNP:rs41276572Ensembl.1
Natural variantiVAR_0612361155D → H1 PublicationCorresponds to variant dbSNP:rs34236116Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015370635 – 676DSCDF…YLYHG → GRQKCLNSLPFPTSFEPPKR GTSSAKKGHLWSYLITLLEN QY in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_015371677 – 1162Missing in isoform 2. 1 PublicationAdd BLAST486

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073771 mRNA Translation: AAC64044.1
AF080255 mRNA Translation: AAD49435.1
AK291017 mRNA Translation: BAF83706.1
AL391476 Genomic DNA No translation available.
AL445231 Genomic DNA No translation available.
BC030058 mRNA Translation: AAH30058.1
CCDSiCCDS892.1 [Q9UNY4-1]
RefSeqiNP_003585.3, NM_003594.3 [Q9UNY4-1]
XP_016858044.1, XM_017002555.1 [Q9UNY4-1]
UniGeneiHs.32333
Hs.486818

Genome annotation databases

EnsembliENST00000369466; ENSP00000358478; ENSG00000116830 [Q9UNY4-1]
GeneIDi8458
KEGGihsa:8458
UCSCiuc001egy.4 human [Q9UNY4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTTF2_HUMAN
AccessioniPrimary (citable) accession number: Q9UNY4
Secondary accession number(s): A8K4Q2
, O75921, Q5T2K7, Q5VVU8, Q8N6I8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: May 23, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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