Q9UNW9 (NOVA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein Nova-2

Alternative name(s):
Astrocytic NOVA1-like RNA-binding protein
Neuro-oncological ventral antigen 2

Gene names

Name:	NOVA2
Synonyms:	ANOVA, NOVA3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate RNA splicing or metabolism in a specific subset of developing neurons By similarity. Binds single strand RNA.
Subunit structure	Interacts with PTBP2; the interaction is direct By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Brain. Expression restricted to astrocytes.
Domain	The third KH domain (KH3) recognizes specifically 5'-UCAY-3'.
Sequence similarities	Contains 3 KH domains.
Sequence caution	The sequence AAB88661.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from direct assay. Source: HPA
Molecular function	RNA binding Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 492

492

RNA-binding protein Nova-2

PRO_0000050119

Regions

Domain

32 – 99

68

KH 1

Domain

130 – 196

67

KH 2

Domain

406 – 473

68

KH 3

Motif

10 – 26

17

Bipartite nuclear localization signal Potential

Compositional bias

232 – 405

174

Ala-rich

Compositional bias

332 – 393

62

Gly-rich

Experimental info

Sequence conflict

247

1

A → R in AAB88661. Ref.1

Sequence conflict

265 – 267

3

PAA → TAT in AAB88661. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

492

Helix Strand Turn

Details...

Beta strand

407 – 414

8

Turn

415 – 417

3

Helix

418 – 422

5

Helix

427 – 436

10

Beta strand

439 – 442

4

Beta strand

454 – 462

9

Helix

463 – 476

14

Helix

481 – 486

6

Sequences

	Sequence		Length	Mass (Da)
	Q9UNW9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 41B63EAF6899256B	FASTA	492	49,009
	10 20 30 40 50 60 MEPEAPDSRK RPLETPPEVV CTKRSNTGEE GEYFLKVLIP SYAAGSIIGK GGQTIVQLQK 70 80 90 100 110 120 ETGATIKLSK SKDFYPGTTE RVCLVQGTAE ALNAVHSFIA EKVREIPQAM TKPEVVNILQ 130 140 150 160 170 180 PQTTMNPDRA KQAKLIVPNS TAGLIIGKGG ATVKAVMEQS GAWVQLSQKP EGINLQERVV 190 200 210 220 230 240 TVSGEPEQVH KAVSAIVQKV QEDPQSSSCL NISYANVAGP VANSNPTGSP YASPADVLPA 250 260 270 280 290 300 AAAASAAAAS GLLGPAGLAG VGAFPAALPA FSGTDLLAIS TALNTLASYG YNTNSLGLGL 310 320 330 340 350 360 NSAAASGVLA AVAAGANPAA AAAANLLASY AGEAGAGPAG GAAPPPPPPP GALGSFALAA 370 380 390 400 410 420 AANGYLGAGA GGGAGGGGGP LVAAAAAAGA AGGFLTAEKL AAESAKELVE IAVPENLVGA 430 440 450 460 470 480 ILGKGGKTLV EYQELTGARI QISKKGEFLP GTRNRRVTIT GSPAATQAAQ YLISQRVTYE 490 QGVRASNPQK VG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ANOVA, a putative astrocytic RNA binding protein gene that maps to chromosome 19q13.3." Ueki K., Ramaswamy S., Billings S.J., Mohrenweiser H.W., Louis D.N. Neurogenetics 1:31-36(1997) [PubMed: 10735272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia." Yang Y.Y., Yin G.L., Darnell R.B. Proc. Natl. Acad. Sci. U.S.A. 95:13254-13259(1998) [PubMed: 9789075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Crystal structures of Nova-1 and Nova-2 K-homology RNA-binding domains." Lewis H.A., Chen H., Edo C., Buckanovich R.J., Yang Y.Y.-L., Musunuru K., Zhong R., Darnell R.B., Burley S.K. Structure 7:191-203(1999) [PubMed: 10368286] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 405-480.
[5]	"Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome." Lewis H.A., Musunuru K., Jensen K.B., Edo C., Chen H., Darnell R.B., Burley S.K. Cell 100:323-332(2000) [PubMed: 10676814] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 405-491 IN COMPLEX WITH RNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U70477 mRNA. Translation: AAB88661.1. Different initiation.
AF083898 mRNA. Translation: AAC72355.1.
AC006540 Genomic DNA. Translation: AAD13116.1.

IPI

IPI00156005.

RefSeq

NP_002507.1. NM_002516.2.

UniGene

Hs.375439.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DTJ	X-ray	2.00	A/B/C/D	406-480	[»]
1EC6	X-ray	2.40	A/B	406-491	[»]

ProteinModelPortal

Q9UNW9.

SMR

Q9UNW9. Positions 32-205, 406-491.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9UNW9.

Polymorphism databases

DMDM

33516944.

Proteomic databases

PRIDE

Q9UNW9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263257; ENSP00000263257; ENSG00000104967.

GeneID

4858.

KEGG

hsa:4858.

UCSC

uc002pdv.1. human.

Organism-specific databases

CTD

4858.

GeneCards

GC19M046442.

H-InvDB

HIX0040105.

HGNC

HGNC:7887. NOVA2.

HPA

HPA045607.

MIM

601991. gene.

neXtProt

NX_Q9UNW9.

PharmGKB

PA31689.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20044.

GeneTree

ENSGT00550000074311.

HOGENOM

HBG316865.

HOVERGEN

HBG082048.

InParanoid

Q9UNW9.

OMA

AEEGEYF.

PhylomeDB

Q9UNW9.

Gene expression databases

ArrayExpress

Q9UNW9.

Bgee

Q9UNW9.

CleanEx

HS_NOVA2.

Genevestigator

Q9UNW9.

GermOnline

ENSG00000104967. Homo sapiens.

Family and domain databases

InterPro

IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
[Graphical view]

KO

K14944.

Pfam

PF00013. KH_1. 3 hits.
[Graphical view]

SMART

SM00322. KH. 3 hits.
[Graphical view]

PROSITE

PS50084. KH_TYPE_1. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

18718.

SOURCE

Search...

Entry information

Entry name

NOVA2_HUMAN

Accession

Primary (citable) accession number: Q9UNW9
Secondary accession number(s): O43267, Q9UEA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 4, 2003
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.