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Protein

Group IID secretory phospholipase A2

Gene

PLA2G2D

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

pH dependencei

Optimally active at neutral to alkaline pHs with 2 mM Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Calcium; via carbonyl oxygenBy similarity
Metal bindingi49 – 491Calcium; via carbonyl oxygenBy similarity
Metal bindingi51 – 511Calcium; via carbonyl oxygenBy similarity
Active sitei67 – 671By similarity
Metal bindingi68 – 681CalciumBy similarity
Active sitei112 – 1121By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • phospholipase A2 activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120906. Synthesis of PA.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Names & Taxonomyi

Protein namesi
Recommended name:
Group IID secretory phospholipase A2 (EC:3.1.1.4)
Short name:
GIID sPLA2
Short name:
sPLA2-IID
Alternative name(s):
PLA2IID
Phosphatidylcholine 2-acylhydrolase 2D
Secretory-type PLA, stroma-associated homolog
Gene namesi
Name:PLA2G2D
Synonyms:SPLASH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9033. PLA2G2D.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33363.

Polymorphism and mutation databases

DMDMi20139286.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 145125Group IID secretory phospholipase A2PRO_0000022755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 138By similarity
Disulfide bondi48 ↔ 64By similarity
Disulfide bondi63 ↔ 118By similarity
Disulfide bondi69 ↔ 145By similarity
Disulfide bondi70 ↔ 111By similarity
Disulfide bondi79 ↔ 104By similarity
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi97 ↔ 109By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9UNK4.
PRIDEiQ9UNK4.

PTM databases

PhosphoSiteiQ9UNK4.

Expressioni

Tissue specificityi

Broadly expressed.

Gene expression databases

BgeeiQ9UNK4.
CleanExiHS_PLA2G2D.
ExpressionAtlasiQ9UNK4. baseline and differential.
GenevestigatoriQ9UNK4.

Organism-specific databases

HPAiHPA046308.

Interactioni

Protein-protein interaction databases

BioGridi117662. 1 interaction.
STRINGi9606.ENSP00000364246.

Structurei

3D structure databases

ProteinModelPortaliQ9UNK4.
SMRiQ9UNK4. Positions 22-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG290941.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9UNK4.
KOiK01047.
OMAiCACDKEV.
OrthoDBiEOG7N63PF.
PhylomeDBiQ9UNK4.
TreeFamiTF319283.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UNK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELALLCGLV VMAGVIPIQG GILNLNKMVK QVTGKMPILS YWPYGCHCGL
60 70 80 90 100
GGRGQPKDAT DWCCQTHDCC YDHLKTQGCS IYKDYYRYNF SQGNIHCSDK
110 120 130 140
GSWCEQQLCA CDKEVAFCLK RNLDTYQKRL RFYWRPHCRG QTPGC
Length:145
Mass (Da):16,546
Last modified:February 11, 2002 - v2
Checksum:iCF3A49DE516BD1EF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651Q → H.1 Publication
Corresponds to variant rs62541890 [ dbSNP | Ensembl ].
VAR_055387
Natural varianti73 – 731H → R.1 Publication
Corresponds to variant rs62541891 [ dbSNP | Ensembl ].
VAR_055388
Natural varianti80 – 801S → G.3 Publications
Corresponds to variant rs584367 [ dbSNP | Ensembl ].
VAR_012741
Natural varianti96 – 961H → R.1 Publication
Corresponds to variant rs62541892 [ dbSNP | Ensembl ].
VAR_055389
Natural varianti121 – 1211R → C.1 Publication
Corresponds to variant rs62541900 [ dbSNP | Ensembl ].
VAR_055390
Natural varianti121 – 1211R → L.1 Publication
VAR_055391

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112982 mRNA. Translation: AAD51390.1.
AF188625 mRNA. Translation: AAF09020.1.
AK290406 mRNA. Translation: BAF83095.1.
EU447440 Genomic DNA. Translation: ACA06110.1.
AL158172 Genomic DNA. Translation: CAC13159.1.
BC025706 mRNA. Translation: AAH25706.1.
CCDSiCCDS203.1.
RefSeqiNP_001258743.1. NM_001271814.1.
NP_036532.1. NM_012400.3.
UniGeneiHs.189507.

Genome annotation databases

EnsembliENST00000375105; ENSP00000364246; ENSG00000117215.
GeneIDi26279.
KEGGihsa:26279.
UCSCiuc001bcz.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112982 mRNA. Translation: AAD51390.1.
AF188625 mRNA. Translation: AAF09020.1.
AK290406 mRNA. Translation: BAF83095.1.
EU447440 Genomic DNA. Translation: ACA06110.1.
AL158172 Genomic DNA. Translation: CAC13159.1.
BC025706 mRNA. Translation: AAH25706.1.
CCDSiCCDS203.1.
RefSeqiNP_001258743.1. NM_001271814.1.
NP_036532.1. NM_012400.3.
UniGeneiHs.189507.

3D structure databases

ProteinModelPortaliQ9UNK4.
SMRiQ9UNK4. Positions 22-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117662. 1 interaction.
STRINGi9606.ENSP00000364246.

Chemistry

BindingDBiQ9UNK4.
ChEMBLiCHEMBL4281.

PTM databases

PhosphoSiteiQ9UNK4.

Polymorphism and mutation databases

DMDMi20139286.

Proteomic databases

PaxDbiQ9UNK4.
PRIDEiQ9UNK4.

Protocols and materials databases

DNASUi26279.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375105; ENSP00000364246; ENSG00000117215.
GeneIDi26279.
KEGGihsa:26279.
UCSCiuc001bcz.4. human.

Organism-specific databases

CTDi26279.
GeneCardsiGC01M020438.
HGNCiHGNC:9033. PLA2G2D.
HPAiHPA046308.
MIMi605630. gene.
neXtProtiNX_Q9UNK4.
PharmGKBiPA33363.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG290941.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9UNK4.
KOiK01047.
OMAiCACDKEV.
OrthoDBiEOG7N63PF.
PhylomeDBiQ9UNK4.
TreeFamiTF319283.

Enzyme and pathway databases

ReactomeiREACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120906. Synthesis of PA.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Miscellaneous databases

ChiTaRSiPLA2G2D. human.
GeneWikiiPLA2G2D.
GenomeRNAii26279.
NextBioi48597.
PROiQ9UNK4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UNK4.
CleanExiHS_PLA2G2D.
ExpressionAtlasiQ9UNK4. baseline and differential.
GenevestigatoriQ9UNK4.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of novel mouse and human secretory phospholipase A2s."
    Ishizaki J., Suzuki N., Higashino K., Yokota Y., Ono T., Kawamoto K., Fujii N., Arita H., Hanasaki K.
    J. Biol. Chem. 274:24973-24979(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-80, CHARACTERIZATION.
  2. "SPLASH (PLA(2)IID), a novel member of phospholipase A2 family, is associated with lymphotoxin-deficiency."
    Shakhov A.N., Rubtsov A.V., Lyakhov I.G., Tumanov A.V., Nedospasov S.A.
    Genes Immun. 1:191-199(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-80.
    Tissue: Umbilical cord blood.
  4. NIEHS SNPs program
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-65; ARG-73; GLY-80; ARG-96; CYS-121 AND LEU-121.
  5. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas and Spleen.

Entry informationi

Entry nameiPA2GD_HUMAN
AccessioniPrimary (citable) accession number: Q9UNK4
Secondary accession number(s): A8K2Z1, B1AEL9, Q9UK01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: February 11, 2002
Last modified: May 27, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.