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Protein

Syntaxin-8

Gene

STX8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.

GO - Molecular functioni

  1. chloride channel inhibitor activity Source: UniProtKB
  2. SNAP receptor activity Source: GO_Central
  3. syntaxin binding Source: UniProtKB

GO - Biological processi

  1. early endosome to late endosome transport Source: UniProtKB
  2. endosome to lysosome transport Source: Ensembl
  3. intracellular protein transport Source: GO_Central
  4. regulation of protein localization to plasma membrane Source: UniProtKB
  5. transport Source: ProtInc
  6. vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-8
Gene namesi
Name:STX8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:11443. STX8.

Subcellular locationi

Membrane By similarity; Single-pass type IV membrane protein By similarity
Note: Preferentially associated with the early endosome. To lesser extends, also present in late endosome, the plasma membrane and coated pits (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 215215CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei216 – 23217Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini233 – 2364VesicularSequence Analysis

GO - Cellular componenti

  1. early endosome Source: UniProtKB
  2. endoplasmic reticulum Source: ProtInc
  3. endosome Source: UniProtKB
  4. integral component of plasma membrane Source: ProtInc
  5. late endosome Source: UniProtKB
  6. late endosome membrane Source: Ensembl
  7. lysosomal membrane Source: Ensembl
  8. perinuclear region of cytoplasm Source: UniProtKB
  9. recycling endosome Source: UniProtKB
  10. SNARE complex Source: GO_Central
  11. trans-Golgi network Source: UniProtKB
  12. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36240.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Syntaxin-8PRO_0000210217Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei160 – 1601PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by HECTD3.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9UNK0.
PaxDbiQ9UNK0.
PRIDEiQ9UNK0.

PTM databases

PhosphoSiteiQ9UNK0.

Expressioni

Tissue specificityi

Highly expressed in heart. Also found in brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

Gene expression databases

BgeeiQ9UNK0.
CleanExiHS_STX8.
ExpressionAtlasiQ9UNK0. baseline and differential.
GenevestigatoriQ9UNK0.

Organism-specific databases

HPAiHPA001540.

Interactioni

Subunit structurei

Forms a SNARE complex with STX7, VTI1B and VAMP8 which functions in the homotypic fusion of late endosomes. Part of the SNARE core complex containing STX7, VAMP8 and VTI1B. Interacts with VAMP8 (By similarity). Interacts with HECTD3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi114867. 25 interactions.
DIPiDIP-47274N.
IntActiQ9UNK0. 17 interactions.
MINTiMINT-1396123.
STRINGi9606.ENSP00000305255.

Structurei

3D structure databases

ProteinModelPortaliQ9UNK0.
SMRiQ9UNK0. Positions 152-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 20763t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili42 – 6524Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259218.
GeneTreeiENSGT00390000007779.
HOGENOMiHOG000031515.
HOVERGENiHBG007572.
InParanoidiQ9UNK0.
KOiK08501.
OMAiNQYERNG.
PhylomeDBiQ9UNK0.
TreeFamiTF323262.

Family and domain databases

InterProiIPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
PROSITEiPS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UNK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPDPWFSTY DSTCQIAQEI AEKIQQRNQY ERKGEKAPKL TVTIRALLQN
60 70 80 90 100
LKEKIALLKD LLLRAVSTHQ ITQLEGDRRQ NLLDDLVTRE RLLLASFKNE
110 120 130 140 150
GAEPDLIRSS LMSEEAKRGA PNPWLFEEPE ETRGLGFDEI RQQQQKIIQE
160 170 180 190 200
QDAGLDALSS IISRQKQMGQ EIGNELDEQN EIIDDLANLV ENTDEKLRNE
210 220 230
TRRVNMVDRK SASCGMIMVI LLLLVAIVVV AVWPTN
Length:236
Mass (Da):26,907
Last modified:December 1, 2000 - v2
Checksum:i1E64A0DA77584CC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781R → Q in AAD20831 (PubMed:9852078).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115323 mRNA. Translation: AAD20831.1.
AF062077 mRNA. Translation: AAC36466.1.
AF036715 mRNA. Translation: AAC95285.1.
BT007319 mRNA. Translation: AAP35983.1.
CH471108 Genomic DNA. Translation: EAW90029.1.
BC009713 mRNA. Translation: AAH09713.1.
CCDSiCCDS32565.1.
RefSeqiNP_004844.1. NM_004853.2.
UniGeneiHs.431109.

Genome annotation databases

EnsembliENST00000306357; ENSP00000305255; ENSG00000170310.
GeneIDi9482.
KEGGihsa:9482.
UCSCiuc002glx.3. human.

Polymorphism databases

DMDMi9297054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115323 mRNA. Translation: AAD20831.1.
AF062077 mRNA. Translation: AAC36466.1.
AF036715 mRNA. Translation: AAC95285.1.
BT007319 mRNA. Translation: AAP35983.1.
CH471108 Genomic DNA. Translation: EAW90029.1.
BC009713 mRNA. Translation: AAH09713.1.
CCDSiCCDS32565.1.
RefSeqiNP_004844.1. NM_004853.2.
UniGeneiHs.431109.

3D structure databases

ProteinModelPortaliQ9UNK0.
SMRiQ9UNK0. Positions 152-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114867. 25 interactions.
DIPiDIP-47274N.
IntActiQ9UNK0. 17 interactions.
MINTiMINT-1396123.
STRINGi9606.ENSP00000305255.

PTM databases

PhosphoSiteiQ9UNK0.

Polymorphism databases

DMDMi9297054.

Proteomic databases

MaxQBiQ9UNK0.
PaxDbiQ9UNK0.
PRIDEiQ9UNK0.

Protocols and materials databases

DNASUi9482.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306357; ENSP00000305255; ENSG00000170310.
GeneIDi9482.
KEGGihsa:9482.
UCSCiuc002glx.3. human.

Organism-specific databases

CTDi9482.
GeneCardsiGC17M009153.
HGNCiHGNC:11443. STX8.
HPAiHPA001540.
MIMi604203. gene.
neXtProtiNX_Q9UNK0.
PharmGKBiPA36240.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG259218.
GeneTreeiENSGT00390000007779.
HOGENOMiHOG000031515.
HOVERGENiHBG007572.
InParanoidiQ9UNK0.
KOiK08501.
OMAiNQYERNG.
PhylomeDBiQ9UNK0.
TreeFamiTF323262.

Miscellaneous databases

ChiTaRSiSTX8. human.
GeneWikiiSTX8.
GenomeRNAii9482.
NextBioi35540.
PROiQ9UNK0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UNK0.
CleanExiHS_STX8.
ExpressionAtlasiQ9UNK0. baseline and differential.
GenevestigatoriQ9UNK0.

Family and domain databases

InterProiIPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
PROSITEiPS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)."
    Thoreau V., Berges T., Callebaut I., Guillier-Gencik Z., Gressin L., Bernheim A., Karst F., Mornon J.-P., Kitzis A., Chomel J.-C.
    Biochem. Biophys. Res. Commun. 257:577-583(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  3. Subramaniam V.N., Loh E., Hong W.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTX8_HUMAN
AccessioniPrimary (citable) accession number: Q9UNK0
Secondary accession number(s): O60712, Q53XT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 4, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.