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Protein

Dual specificity protein phosphatase 12

Gene

DUSP12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro) (By similarity). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PubMed:10446167, PubMed:24531476).By similarity2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarity
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.By similarity

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • phosphatase activity Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: Ensembl
  • protein tyrosine phosphatase activity Source: ProtInc
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular protein modification process Source: ProtInc
  • dephosphorylation Source: UniProtKB
  • positive regulation of glucokinase activity Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS01399-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 12 (EC:3.1.3.16By similarity, EC:3.1.3.48By similarity)
Alternative name(s):
Dual specificity tyrosine phosphatase YVH1
Gene namesi
Name:DUSP12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3067. DUSP12.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasmcytosol 1 Publication

  • Note: Primarily nuclear. Detected in a mesh-like pattern in the cytosol.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11266.
OpenTargetsiENSG00000081721.
PharmGKBiPA27522.

Polymorphism and mutation databases

BioMutaiDUSP12.
DMDMi9973073.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948181 – 340Dual specificity protein phosphatase 12Add BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei335PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UNI6.
MaxQBiQ9UNI6.
PaxDbiQ9UNI6.
PeptideAtlasiQ9UNI6.
PRIDEiQ9UNI6.

PTM databases

DEPODiQ9UNI6.
iPTMnetiQ9UNI6.
PhosphoSitePlusiQ9UNI6.

Expressioni

Tissue specificityi

Ubiquitous, highest expression in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.

Gene expression databases

BgeeiENSG00000081721.
CleanExiHS_DUSP12.
ExpressionAtlasiQ9UNI6. baseline and differential.
GenevisibleiQ9UNI6. HS.

Organism-specific databases

HPAiHPA008840.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131374EBI-715161,EBI-739580
CCNHP519463EBI-715161,EBI-741406
EIF5P550105EBI-715161,EBI-286450
HSPB8Q9UJY13EBI-715161,EBI-739074
PPP2R3BQ9Y5P84EBI-715161,EBI-2479826
RABAC1Q9UI146EBI-715161,EBI-712367
USO1O607633EBI-715161,EBI-356164

Protein-protein interaction databases

BioGridi116424. 14 interactors.
IntActiQ9UNI6. 11 interactors.
MINTiMINT-1415234.
STRINGi9606.ENSP00000356920.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 31Combined sources4
Beta strandi34 – 37Combined sources4
Helixi39 – 43Combined sources5
Helixi45 – 51Combined sources7
Beta strandi53 – 62Combined sources10
Beta strandi76 – 81Combined sources6
Helixi91 – 93Combined sources3
Helixi94 – 106Combined sources13
Beta strandi110 – 114Combined sources5
Beta strandi116 – 120Combined sources5
Helixi121 – 134Combined sources14
Helixi138 – 148Combined sources11
Helixi156 – 167Combined sources12
Helixi177 – 186Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JNBX-ray3.00A27-193[»]
4KI9X-ray2.00A27-189[»]
ProteinModelPortaliQ9UNI6.
SMRiQ9UNI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 160Tyrosine-protein phosphataseAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 121Substrate bindingCombined sources1 Publication6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00740000115610.
HOGENOMiHOG000243638.
HOVERGENiHBG051421.
InParanoidiQ9UNI6.
KOiK14819.
OMAiFAWQGMQ.
OrthoDBiEOG091G0QP2.
PhylomeDBiQ9UNI6.
TreeFamiTF105123.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PIRSFiPIRSF000941. DUSP12. 1 hit.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UNI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEAPGPSDG CELSNPSASR VSCAGQMLEV QPGLYFGGAA AVAEPDHLRE
60 70 80 90 100
AGITAVLTVD SEEPSFKAGP GVEDLWRLFV PALDKPETDL LSHLDRCVAF
110 120 130 140 150
IGQARAEGRA VLVHCHAGVS RSVAIITAFL MKTDQLPFEK AYEKLQILKP
160 170 180 190 200
EAKMNEGFEW QLKLYQAMGY EVDTSSAIYK QYRLQKVTEK YPELQNLPQE
210 220 230 240 250
LFAVDPTTVS QGLKDEVLYK CRKCRRSLFR SSSILDHREG SGPIAFAHKR
260 270 280 290 300
MTPSSMLTTG RQAQCTSYFI EPVQWMESAL LGVMDGQLLC PKCSAKLGSF
310 320 330 340
NWYGEQCSCG RWITPAFQIH KNRVDEMKIL PVLGSQTGKI
Length:340
Mass (Da):37,687
Last modified:May 1, 2000 - v1
Checksum:i56B52192B42C73EB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03389951A → E.Corresponds to variant rs35106830dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119226 mRNA. Translation: AAD51134.1.
BT006633 mRNA. Translation: AAP35279.1.
AL359541 Genomic DNA. Translation: CAH74153.1.
BC006286 mRNA. Translation: AAH06286.1.
CCDSiCCDS1234.1.
RefSeqiNP_009171.1. NM_007240.2.
UniGeneiHs.416216.

Genome annotation databases

EnsembliENST00000367943; ENSP00000356920; ENSG00000081721.
GeneIDi11266.
KEGGihsa:11266.
UCSCiuc001gbo.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119226 mRNA. Translation: AAD51134.1.
BT006633 mRNA. Translation: AAP35279.1.
AL359541 Genomic DNA. Translation: CAH74153.1.
BC006286 mRNA. Translation: AAH06286.1.
CCDSiCCDS1234.1.
RefSeqiNP_009171.1. NM_007240.2.
UniGeneiHs.416216.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JNBX-ray3.00A27-193[»]
4KI9X-ray2.00A27-189[»]
ProteinModelPortaliQ9UNI6.
SMRiQ9UNI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116424. 14 interactors.
IntActiQ9UNI6. 11 interactors.
MINTiMINT-1415234.
STRINGi9606.ENSP00000356920.

PTM databases

DEPODiQ9UNI6.
iPTMnetiQ9UNI6.
PhosphoSitePlusiQ9UNI6.

Polymorphism and mutation databases

BioMutaiDUSP12.
DMDMi9973073.

Proteomic databases

EPDiQ9UNI6.
MaxQBiQ9UNI6.
PaxDbiQ9UNI6.
PeptideAtlasiQ9UNI6.
PRIDEiQ9UNI6.

Protocols and materials databases

DNASUi11266.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367943; ENSP00000356920; ENSG00000081721.
GeneIDi11266.
KEGGihsa:11266.
UCSCiuc001gbo.4. human.

Organism-specific databases

CTDi11266.
DisGeNETi11266.
GeneCardsiDUSP12.
HGNCiHGNC:3067. DUSP12.
HPAiHPA008840.
MIMi604835. gene.
neXtProtiNX_Q9UNI6.
OpenTargetsiENSG00000081721.
PharmGKBiPA27522.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00740000115610.
HOGENOMiHOG000243638.
HOVERGENiHBG051421.
InParanoidiQ9UNI6.
KOiK14819.
OMAiFAWQGMQ.
OrthoDBiEOG091G0QP2.
PhylomeDBiQ9UNI6.
TreeFamiTF105123.

Enzyme and pathway databases

BioCyciZFISH:HS01399-MONOMER.

Miscellaneous databases

GeneWikiiDUSP12.
GenomeRNAii11266.
PROiQ9UNI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000081721.
CleanExiHS_DUSP12.
ExpressionAtlasiQ9UNI6. baseline and differential.
GenevisibleiQ9UNI6. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PIRSFiPIRSF000941. DUSP12. 1 hit.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS12_HUMAN
AccessioniPrimary (citable) accession number: Q9UNI6
Secondary accession number(s): Q5VXA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.