Q9UNH7 (SNX6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorting nexin-6

Alternative name(s):
TRAF4-associated factor 2

Gene names

Name:

SNX6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in several stages of intracellular trafficking. Promotes lysosomal degradation of CDKN1B By similarity. Plays a role in retrograde protein transport from endosomes to the trans-Golgi network. May function as link between transport vesicles and dynactin. Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network. May contribute to transcription regulation. Ref.5 Ref.6 Ref.7
Subunit structure	Interacts with CDKN1B By similarity. Interacts with TGFB receptors. Interacts with dynactin subunit DCTN1. Interacts with SNX1 and SNX2. Interacts with BACE1 and BRMS1. Ref.4 Ref.5 Ref.6 Ref.7
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Nucleus. Note: Interaction with SNX1 or SNX2 promotes location at endosome membranes. Only a minor proportion is seen in the nucleus. Ref.1 Ref.5 Ref.6 Ref.7
Domain	The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate By similarity.
Sequence similarities	Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence AAD24202.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Endosome Membrane Nucleus
Ligand	Lipid-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro intracellular protein transport Inferred from mutant phenotype Ref.5. Source: UniProtKB negative regulation of epidermal growth factor-activated receptor activity Non-traceable author statement Ref.4. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.7. Source: UniProtKB negative regulation of transforming growth factor beta receptor signaling pathway Inferred from direct assay Ref.4. Source: HGNC retrograde transport, endosome to Golgi Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome membrane Inferred from direct assay Ref.5. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylinositol binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from physical interaction Ref.4. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Sorting nexin-6		PRO_0000213846
Regions
	Domain	26 – 173	148	PX
	Region	41 – 47	7	Phosphatidylinositol bisphosphate binding By similarity
	Region	100 – 106	7	Phosphatidylinositol bisphosphate binding By similarity
	Region	114 – 117	4	Phosphatidylinositol bisphosphate binding By similarity
Experimental info
	Mutagenesis	68	1	R → A: Reduces interaction with SNX1. Abolishes location at endosome membranes. Ref.5
	Mutagenesis	69	1	Q → A: No effect on subcellular location. Ref.5
	Mutagenesis	97	1	R → A: No effect on subcellular location. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9UNH7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E3659DB19C59E1BB	FASTA	406	46,649
	10 20 30 40 50 60 MMEGLDDGPD FLSEEDRGLK AINVDLQSDA ALQVDISDAL SERDKVKFTV HTKSSLPNFK 70 80 90 100 110 120 QNEFSVVRQH EEFIWLHDSF VENEDYAGYI IPPAPPRPDF DASREKLQKL GEGEGSMTKE 130 140 150 160 170 180 EFTKMKQELE AEYLAIFKKT VAMHEVFLCR VAAHPILRRD LNFHVFLEYN QDLSVRGKNK 190 200 210 220 230 240 KEKLEDFFKN MVKSADGVIV SGVKDVDDFF EHERTFLLEY HNRVKDASAK SDRMTRSHKS 250 260 270 280 290 300 AADDYNRIGS SLYALGTQDS TDICKFFLKV SELFDKTRKI EARVSADEDL KLSDLLKYYL 310 320 330 340 350 360 RESQAAKDLL YRRSRSLVDY ENANKALDKA RAKNKDVLQA ETSQQLCCQK FEKISESAKQ 370 380 390 400 ELIDFKTRRV AAFRKNLVEL AELELKHAKG NLQLLQNCLA VLNGDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large family of endosome-localized proteins related to sorting nexin 1." Teasdale R.D., Loci D., Houghton F., Karlsson L., Gleeson P.A. Biochem. J. 358:7-16(2001) [PubMed: 11485546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	Toji S., Yano M., Kobayasi A., Tamai K. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Sorting nexin 6, a novel SNX, interacts with the transforming growth factor-beta family of receptor serine-threonine kinases." Parks W.T., Frank D.B., Huff C., Haft C.R., Martin J., Meng X., de Caestecker M.P., McNally J.G., Reddi A., Taylor S.I., Roberts A.B., Wang T., Lechleider R.J. J. Biol. Chem. 276:19332-19339(2001) [PubMed: 11279102] [Abstract] Cited for: CHARACTERIZATION, SUBUNIT.
[5]	"The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport." Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J. Cell Res. 19:1334-1349(2009) [PubMed: 19935774] [Abstract] Cited for: FUNCTION, INTERACTION WITH DCTN1; SNX1 AND SNX2, MUTAGENESIS OF ARG-68; GLN-69 AND ARG-97, SUBCELLULAR LOCATION.
[6]	"Proteomic identification of sorting nexin 6 as a negative regulator of BACE1-mediated APP processing." Okada H., Zhang W., Peterhoff C., Hwang J.C., Nixon R.A., Ryu S.H., Kim T.W. FASEB J. 24:2783-2794(2010) [PubMed: 20354142] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BACE1.
[7]	"Sorting nexin 6 interacts with breast cancer metastasis suppressor-1 and promotes transcriptional repression." Rivera J., Megias D., Bravo J. J. Cell. Biochem. 111:1464-1472(2010) [PubMed: 20830743] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BRMS1.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF121856 mRNA. Translation: AAD27829.1. U83194 mRNA. Translation: AAD24202.1. Different initiation. AL445363 Genomic DNA. No translation available. AL445883 Genomic DNA. No translation available.
IPI	IPI00298111.
RefSeq	NP_067072.3. NM_021249.3. NP_689419.2. NM_152233.2.
UniGene	Hs.356647.
3D structure databases
ProteinModelPortal	Q9UNH7.
SMR	Q9UNH7. Positions 21-175, 207-404.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UNH7. 11 interactions.
MINT	MINT-2634416.
STRING	Q9UNH7.
Polymorphism databases
DMDM	10720285.
2D gel databases
OGP	Q9UNH7.
REPRODUCTION-2DPAGE	IPI00298111.
Proteomic databases
PRIDE	Q9UNH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000362031; ENSP00000355217; ENSG00000129515.
GeneID	58533.
KEGG	hsa:58533.
Organism-specific databases
CTD	58533.
GeneCards	GC14M035030.
H-InvDB	HIX0011592.
HGNC	HGNC:14970. SNX6.
MIM	606098. gene.
neXtProt	NX_Q9UNH7.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074512.
HOGENOM	HBG714193.
HOVERGEN	HBG000716.
InParanoid	Q9UNH7.
PhylomeDB	Q9UNH7.
Gene expression databases
ArrayExpress	Q9UNH7.
Bgee	Q9UNH7.
CleanEx	HS_SNX6.
Genevestigator	Q9UNH7.
GermOnline	ENSG00000129515. Homo sapiens.
Family and domain databases
InterPro	IPR001683. Phox. IPR014637. Snx5_Snx6. IPR015404. Vps5_C. [Graphical view]
Gene3D	G3DSA:3.30.1520.10. PX. 1 hit.
Pfam	PF00787. PX. 1 hit. PF09325. Vps5. 1 hit. [Graphical view]
PIRSF	PIRSF036924. Snx5_Snx6. 1 hit.
SUPFAM	SSF64268. PX. 1 hit.
PROSITE	PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	65116.
SOURCE	Search...

Entry information

Entry name

SNX6_HUMAN

Accession

Primary (citable) accession number: Q9UNH7
Secondary accession number(s): Q9Y449

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.