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Q9UNH7 (SNX6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorting nexin-6
Alternative name(s):
TRAF4-associated factor 2

Cleaved into the following chain:

  1. Sorting nexin-6, N-terminally processed
Gene names
Name:SNX6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in several stages of intracellular trafficking. Promotes lysosomal degradation of CDKN1B By similarity. Plays a role in retrograde protein transport from endosomes to the trans-Golgi network. May function as link between transport vesicles and dynactin. Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network. May contribute to transcription regulation. Ref.8 Ref.9 Ref.10

Subunit structure

Interacts with CDKN1B By similarity. Interacts with TGFB receptors. Interacts with dynactin subunit DCTN1. Interacts with SNX1 and SNX2. Interacts with BACE1 and BRMS1. Ref.6 Ref.8 Ref.9 Ref.10

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Nucleus. Note: Interaction with SNX1 or SNX2 promotes location at endosome membranes. Only a minor proportion is seen in the nucleus. Ref.1 Ref.8 Ref.9 Ref.10

Domain

The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate By similarity.

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Sequence caution

The sequence AAD24202.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Cytoplasmic vesicle
Endosome
Membrane
Nucleus
   Coding sequence diversityAlternative splicing
   LigandLipid-binding
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular protein transport

Inferred from mutant phenotype Ref.8. Source: UniProtKB

negative regulation of epidermal growth factor-activated receptor activity

Non-traceable author statement Ref.6. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.10. Source: UniProtKB

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from direct assay Ref.6. Source: HGNC

retrograde transport, endosome to Golgi

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.10. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome membrane

Inferred from direct assay Ref.8. Source: UniProtKB

intracellular

Non-traceable author statement Ref.6. Source: UniProtKB

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidylinositol binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction PubMed 11278302Ref.6Ref.8Ref.10. Source: UniProtKB

protein homodimerization activity

Inferred from physical interaction Ref.6. Source: HGNC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OPTNQ96CV92EBI-949294,EBI-748974

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UNH7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UNH7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Sorting nexin-6
PRO_0000423277
Initiator methionine11Removed; alternate Ref.7
Chain2 – 406405Sorting nexin-6, N-terminally processed
PRO_0000213846

Regions

Domain26 – 173148PX
Region41 – 477Phosphatidylinositol bisphosphate binding By similarity
Region100 – 1067Phosphatidylinositol bisphosphate binding By similarity
Region114 – 1174Phosphatidylinositol bisphosphate binding By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7 Ref.12
Modified residue21N-acetylmethionine; in Sorting nexin-6, N-terminally processed Ref.7 Ref.12

Natural variations

Alternative sequence1 – 116116Missing in isoform 2.
VSP_044824

Experimental info

Mutagenesis681R → A: Reduces interaction with SNX1. Abolishes location at endosome membranes. Ref.8
Mutagenesis691Q → A: No effect on subcellular location. Ref.8
Mutagenesis971R → A: No effect on subcellular location. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: E3659DB19C59E1BB

FASTA40646,649
        10         20         30         40         50         60 
MMEGLDDGPD FLSEEDRGLK AINVDLQSDA ALQVDISDAL SERDKVKFTV HTKSSLPNFK 

        70         80         90        100        110        120 
QNEFSVVRQH EEFIWLHDSF VENEDYAGYI IPPAPPRPDF DASREKLQKL GEGEGSMTKE 

       130        140        150        160        170        180 
EFTKMKQELE AEYLAIFKKT VAMHEVFLCR VAAHPILRRD LNFHVFLEYN QDLSVRGKNK 

       190        200        210        220        230        240 
KEKLEDFFKN MVKSADGVIV SGVKDVDDFF EHERTFLLEY HNRVKDASAK SDRMTRSHKS 

       250        260        270        280        290        300 
AADDYNRIGS SLYALGTQDS TDICKFFLKV SELFDKTRKI EARVSADEDL KLSDLLKYYL 

       310        320        330        340        350        360 
RESQAAKDLL YRRSRSLVDY ENANKALDKA RAKNKDVLQA ETSQQLCCQK FEKISESAKQ 

       370        380        390        400 
ELIDFKTRRV AAFRKNLVEL AELELKHAKG NLQLLQNCLA VLNGDT 

« Hide

Isoform 2 [UniParc].

Checksum: C12EC83110C71CA8
Show »

FASTA29033,570

References

« Hide 'large scale' references
[1]"A large family of endosome-localized proteins related to sorting nexin 1."
Teasdale R.D., Loci D., Houghton F., Karlsson L., Gleeson P.A.
Biochem. J. 358:7-16(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
[2]Toji S., Yano M., Kobayasi A., Tamai K.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Eye.
[6]"Sorting nexin 6, a novel SNX, interacts with the transforming growth factor-beta family of receptor serine-threonine kinases."
Parks W.T., Frank D.B., Huff C., Haft C.R., Martin J., Meng X., de Caestecker M.P., McNally J.G., Reddi A., Taylor S.I., Roberts A.B., Wang T., Lechleider R.J.
J. Biol. Chem. 276:19332-19339(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, SUBUNIT.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1 AND MET-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[8]"The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport."
Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J.
Cell Res. 19:1334-1349(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH DCTN1; SNX1 AND SNX2, MUTAGENESIS OF ARG-68; GLN-69 AND ARG-97, SUBCELLULAR LOCATION.
[9]"Proteomic identification of sorting nexin 6 as a negative regulator of BACE1-mediated APP processing."
Okada H., Zhang W., Peterhoff C., Hwang J.C., Nixon R.A., Ryu S.H., Kim T.W.
FASEB J. 24:2783-2794(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BACE1.
[10]"Sorting nexin 6 interacts with breast cancer metastasis suppressor-1 and promotes transcriptional repression."
Rivera J., Megias D., Bravo J.
J. Cell. Biochem. 111:1464-1472(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BRMS1.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1 AND MET-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF121856 mRNA. Translation: AAD27829.1.
U83194 mRNA. Translation: AAD24202.1. Different initiation.
AL445363 Genomic DNA. No translation available.
AL445883 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65913.1.
CH471078 Genomic DNA. Translation: EAW65915.1.
BC001798 mRNA. Translation: AAH01798.1.
CCDSCCDS9648.1. [Q9UNH7-2]
RefSeqNP_067072.3. NM_021249.3. [Q9UNH7-2]
NP_689419.2. NM_152233.2.
UniGeneHs.356647.

3D structure databases

ProteinModelPortalQ9UNH7.
SMRQ9UNH7. Positions 21-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121852. 40 interactions.
DIPDIP-37549N.
IntActQ9UNH7. 15 interactions.
MINTMINT-2634416.
STRING9606.ENSP00000355217.

PTM databases

PhosphoSiteQ9UNH7.

Polymorphism databases

DMDM10720285.

2D gel databases

OGPQ9UNH7.
REPRODUCTION-2DPAGEIPI00298111.

Proteomic databases

MaxQBQ9UNH7.
PaxDbQ9UNH7.
PRIDEQ9UNH7.

Protocols and materials databases

DNASU58533.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000396526; ENSP00000379779; ENSG00000129515. [Q9UNH7-2]
ENST00000396534; ENSP00000379785; ENSG00000129515. [Q9UNH7-2]
GeneID58533.
KEGGhsa:58533.
UCSCuc001wse.1. human. [Q9UNH7-1]

Organism-specific databases

CTD58533.
GeneCardsGC14M035030.
H-InvDBHIX0011592.
HGNCHGNC:14970. SNX6.
HPAHPA049374.
MIM606098. gene.
neXtProtNX_Q9UNH7.
PharmGKBPA37946.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255198.
HOGENOMHOG000231691.
HOVERGENHBG000716.
InParanoidQ9UNH7.
KOK17920.
OrthoDBEOG7X0VH4.
PhylomeDBQ9UNH7.
TreeFamTF313698.

Enzyme and pathway databases

SignaLinkQ9UNH7.

Gene expression databases

ArrayExpressQ9UNH7.
BgeeQ9UNH7.
CleanExHS_SNX6.
GenevestigatorQ9UNH7.

Family and domain databases

Gene3D1.20.1270.60. 1 hit.
3.30.1520.10. 1 hit.
InterProIPR027267. AH/BAR-dom.
IPR001683. Phox.
IPR014637. SNX5/SNX6/SNX32.
IPR028657. SNX6.
IPR015404. Vps5_C.
[Graphical view]
PANTHERPTHR10555:SF120. PTHR10555:SF120. 1 hit.
PfamPF00787. PX. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
PIRSFPIRSF036924. Snx5_Snx6. 1 hit.
SUPFAMSSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSNX6. human.
GenomeRNAi58533.
NextBio65116.
PROQ9UNH7.
SOURCESearch...

Entry information

Entry nameSNX6_HUMAN
AccessionPrimary (citable) accession number: Q9UNH7
Secondary accession number(s): C0H5W9, Q9Y449
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM