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Protein

Protein kinase C and casein kinase substrate in neurons protein 2

Gene

PACSIN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.5 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • identical protein binding Source: IntAct
  • lipid binding Source: UniProtKB-KW
  • phosphatidic acid binding Source: UniProtKB
  • transporter activity Source: ProtInc

GO - Biological processi

  • actin cytoskeleton organization Source: ProtInc
  • caveola assembly Source: UniProtKB
  • caveolin-mediated endocytosis Source: UniProtKB
  • cell projection morphogenesis Source: UniProtKB
  • membrane tubulation Source: UniProtKB
  • negative regulation of endocytosis Source: Ensembl
  • protein localization to endosome Source: UniProtKB
  • regulation of endocytosis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100266-MONOMER.
ReactomeiR-HSA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 2
Alternative name(s):
Syndapin-2
Syndapin-II
Gene namesi
Name:PACSIN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:8571. PACSIN2.

Subcellular locationi

GO - Cellular componenti

  • caveola Source: UniProtKB
  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: HPA
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Ensembl
  • early endosome Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • extrinsic component of membrane Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
  • recycling endosome membrane Source: UniProtKB
  • ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi11252.
OpenTargetsiENSG00000100266.
PharmGKBiPA32897.

Polymorphism and mutation databases

BioMutaiPACSIN2.
DMDMi22256968.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617951 – 486Protein kinase C and casein kinase substrate in neurons protein 2Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei273PhosphoserineCombined sources1
Modified residuei399PhosphoserineBy similarity1
Modified residuei446PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UNF0.
PaxDbiQ9UNF0.
PeptideAtlasiQ9UNF0.
PRIDEiQ9UNF0.

PTM databases

iPTMnetiQ9UNF0.
PhosphoSitePlusiQ9UNF0.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000100266.
ExpressionAtlasiQ9UNF0. baseline and differential.
GenevisibleiQ9UNF0. HS.

Organism-specific databases

HPAiHPA049854.

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-742503,EBI-742503
DNM2P505704EBI-742503,EBI-346547
FASLGP480234EBI-742503,EBI-495538
MEOX2A4D1275EBI-742503,EBI-10172134
PACSIN1Q9BY117EBI-742503,EBI-721769
TERF1P542742EBI-742503,EBI-710997

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi116413. 48 interactors.
IntActiQ9UNF0. 18 interactors.
MINTiMINT-5005738.
STRINGi9606.ENSP00000263246.

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni21 – 24Combined sources4
Helixi25 – 72Combined sources48
Helixi77 – 106Combined sources30
Helixi108 – 119Combined sources12
Beta strandi126 – 128Combined sources3
Helixi129 – 169Combined sources41
Helixi172 – 177Combined sources6
Helixi185 – 188Combined sources4
Helixi189 – 191Combined sources3
Turni192 – 195Combined sources4
Helixi197 – 255Combined sources59
Helixi257 – 259Combined sources3
Helixi263 – 275Combined sources13
Helixi279 – 290Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ABHX-ray2.00A/B1-305[»]
3ACOX-ray2.70A/B1-343[»]
3HAJX-ray2.78A/B1-486[»]
3Q0KX-ray2.60A/B/C/D16-304[»]
ProteinModelPortaliQ9UNF0.
SMRiQ9UNF0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNF0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 282F-BARPROSITE-ProRule annotationAdd BLAST272
Domaini426 – 486SH3PROSITE-ProRule annotationAdd BLAST61

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 274Add BLAST250

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi362 – 364NPF13
Motifi405 – 407NPF23
Motifi417 – 419NPF33

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain.4 Publications

Sequence similaritiesi

Belongs to the PACSIN family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856. Eukaryota.
ENOG410XRX2. LUCA.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ9UNF0.
KOiK20123.
OMAiHHAACKE.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ9UNF0.
TreeFamiTF313677.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23065:SF14. PTHR23065:SF14. 2 hits.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCSD LMNCLHERAR
60 70 80 90 100
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAFMSEA ERVSELHLEV
110 120 130 140 150
KASLMNDDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK
160 170 180 190 200
EVEAAKKAHH AACKEEKLAI SREANSKADP SLNPEQLKKL QDKIEKCKQD
210 220 230 240 250
VLKTKEKYEK SLKELDQGTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE
260 270 280 290 300
VQKHLDLSNV AGYKAIYHDL EQSIRAADAV EDLRWFRANH GPGMAMNWPQ
310 320 330 340 350
FEEWSADLNR TLSRREKKKA TDGVTLTGIN QTGDQSLPSK PSSTLNVPSN
360 370 380 390 400
PAQSAQSQSS YNPFEDEDDT GSTVSEKDDT KAKNVSSYEK TQSYPTDWSD
410 420 430 440 450
DESNNPFSST DANGDSNPFD DDATSGTEVR VRALYDYEGQ EHDELSFKAG
460 470 480
DELTKMEDED EQGWCKGRLD NGQVGLYPAN YVEAIQ
Length:486
Mass (Da):55,739
Last modified:August 13, 2002 - v2
Checksum:i821DBEF65DAD1AA8
GO
Isoform 2 (identifier: Q9UNF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-384: Missing.

Show »
Length:445
Mass (Da):51,353
Checksum:i1DA87A2D42B5D5F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182L → F in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti256D → N in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti309N → I in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti336S → F in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti378 – 380DDT → EDI in AAD41781 (PubMed:10431838).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053555175N → S.Corresponds to variant rs35383004dbSNPEnsembl.1
Natural variantiVAR_013711294M → I.Corresponds to variant rs2746984dbSNPEnsembl.1
Natural variantiVAR_013712324V → F.Corresponds to variant rs1062913dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004517344 – 384Missing in isoform 2. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128536 mRNA. Translation: AAD41781.1.
AL136845 mRNA. Translation: CAB66779.1.
CR456536 mRNA. Translation: CAG30422.1.
AL022476, AL049758 Genomic DNA. Translation: CAI20163.1.
AL049758, AL022476 Genomic DNA. Translation: CAI20948.1.
BC008037 mRNA. Translation: AAH08037.1.
AL389984 mRNA. Translation: CAB97538.1.
CCDSiCCDS43023.1. [Q9UNF0-1]
CCDS54536.1. [Q9UNF0-2]
RefSeqiNP_001171899.1. NM_001184970.1. [Q9UNF0-1]
NP_001171900.1. NM_001184971.1. [Q9UNF0-2]
NP_009160.2. NM_007229.3. [Q9UNF0-1]
XP_005261376.1. XM_005261319.3. [Q9UNF0-2]
XP_016884053.1. XM_017028564.1. [Q9UNF0-1]
UniGeneiHs.162877.

Genome annotation databases

EnsembliENST00000263246; ENSP00000263246; ENSG00000100266. [Q9UNF0-1]
ENST00000337959; ENSP00000338379; ENSG00000100266. [Q9UNF0-2]
ENST00000402229; ENSP00000385040; ENSG00000100266. [Q9UNF0-1]
ENST00000403744; ENSP00000385372; ENSG00000100266. [Q9UNF0-1]
ENST00000407585; ENSP00000385952; ENSG00000100266. [Q9UNF0-2]
GeneIDi11252.
KEGGihsa:11252.
UCSCiuc003bdf.5. human. [Q9UNF0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128536 mRNA. Translation: AAD41781.1.
AL136845 mRNA. Translation: CAB66779.1.
CR456536 mRNA. Translation: CAG30422.1.
AL022476, AL049758 Genomic DNA. Translation: CAI20163.1.
AL049758, AL022476 Genomic DNA. Translation: CAI20948.1.
BC008037 mRNA. Translation: AAH08037.1.
AL389984 mRNA. Translation: CAB97538.1.
CCDSiCCDS43023.1. [Q9UNF0-1]
CCDS54536.1. [Q9UNF0-2]
RefSeqiNP_001171899.1. NM_001184970.1. [Q9UNF0-1]
NP_001171900.1. NM_001184971.1. [Q9UNF0-2]
NP_009160.2. NM_007229.3. [Q9UNF0-1]
XP_005261376.1. XM_005261319.3. [Q9UNF0-2]
XP_016884053.1. XM_017028564.1. [Q9UNF0-1]
UniGeneiHs.162877.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ABHX-ray2.00A/B1-305[»]
3ACOX-ray2.70A/B1-343[»]
3HAJX-ray2.78A/B1-486[»]
3Q0KX-ray2.60A/B/C/D16-304[»]
ProteinModelPortaliQ9UNF0.
SMRiQ9UNF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116413. 48 interactors.
IntActiQ9UNF0. 18 interactors.
MINTiMINT-5005738.
STRINGi9606.ENSP00000263246.

PTM databases

iPTMnetiQ9UNF0.
PhosphoSitePlusiQ9UNF0.

Polymorphism and mutation databases

BioMutaiPACSIN2.
DMDMi22256968.

Proteomic databases

EPDiQ9UNF0.
PaxDbiQ9UNF0.
PeptideAtlasiQ9UNF0.
PRIDEiQ9UNF0.

Protocols and materials databases

DNASUi11252.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263246; ENSP00000263246; ENSG00000100266. [Q9UNF0-1]
ENST00000337959; ENSP00000338379; ENSG00000100266. [Q9UNF0-2]
ENST00000402229; ENSP00000385040; ENSG00000100266. [Q9UNF0-1]
ENST00000403744; ENSP00000385372; ENSG00000100266. [Q9UNF0-1]
ENST00000407585; ENSP00000385952; ENSG00000100266. [Q9UNF0-2]
GeneIDi11252.
KEGGihsa:11252.
UCSCiuc003bdf.5. human. [Q9UNF0-1]

Organism-specific databases

CTDi11252.
DisGeNETi11252.
GeneCardsiPACSIN2.
HGNCiHGNC:8571. PACSIN2.
HPAiHPA049854.
MIMi604960. gene.
neXtProtiNX_Q9UNF0.
OpenTargetsiENSG00000100266.
PharmGKBiPA32897.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2856. Eukaryota.
ENOG410XRX2. LUCA.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ9UNF0.
KOiK20123.
OMAiHHAACKE.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ9UNF0.
TreeFamiTF313677.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100266-MONOMER.
ReactomeiR-HSA-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiPACSIN2. human.
EvolutionaryTraceiQ9UNF0.
GeneWikiiPACSIN2.
GenomeRNAii11252.
PROiQ9UNF0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100266.
ExpressionAtlasiQ9UNF0. baseline and differential.
GenevisibleiQ9UNF0. HS.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23065:SF14. PTHR23065:SF14. 2 hits.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPACN2_HUMAN
AccessioniPrimary (citable) accession number: Q9UNF0
Secondary accession number(s): O95921
, Q96HV9, Q9H0D3, Q9NPN1, Q9Y4V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.