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Protein

Protein kinase C and casein kinase substrate in neurons protein 2

Gene

PACSIN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.5 Publications

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • cytoskeletal protein binding Source: Ensembl
  • identical protein binding Source: IntAct
  • phosphatidic acid binding Source: UniProtKB
  • transporter activity Source: ProtInc

GO - Biological processi

  • actin cytoskeleton organization Source: ProtInc
  • caveola assembly Source: UniProtKB
  • caveolin-mediated endocytosis Source: UniProtKB
  • cell projection morphogenesis Source: UniProtKB
  • membrane organization Source: Reactome
  • negative regulation of endocytosis Source: Ensembl
  • plasma membrane tubulation Source: UniProtKB
  • protein localization to endosome Source: UniProtKB
  • regulation of endocytosis Source: GO_Central

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 2
Alternative name(s):
Syndapin-2
Syndapin-II
Gene namesi
Name:PACSIN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100266.18
HGNCiHGNC:8571 PACSIN2
MIMi604960 gene
neXtProtiNX_Q9UNF0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi11252
OpenTargetsiENSG00000100266
PharmGKBiPA32897

Polymorphism and mutation databases

BioMutaiPACSIN2
DMDMi22256968

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617951 – 486Protein kinase C and casein kinase substrate in neurons protein 2Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei273PhosphoserineCombined sources1
Modified residuei399PhosphoserineBy similarity1
Modified residuei446PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UNF0
PaxDbiQ9UNF0
PeptideAtlasiQ9UNF0
PRIDEiQ9UNF0

PTM databases

iPTMnetiQ9UNF0
PhosphoSitePlusiQ9UNF0

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000100266
ExpressionAtlasiQ9UNF0 baseline and differential
GenevisibleiQ9UNF0 HS

Organism-specific databases

HPAiHPA049854
HPA056520

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • cytoskeletal protein binding Source: Ensembl
  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi11641351 interactors.
IntActiQ9UNF0 20 interactors.
MINTiQ9UNF0
STRINGi9606.ENSP00000263246

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni21 – 24Combined sources4
Helixi25 – 72Combined sources48
Helixi77 – 106Combined sources30
Helixi108 – 119Combined sources12
Beta strandi126 – 128Combined sources3
Helixi129 – 169Combined sources41
Helixi172 – 177Combined sources6
Helixi185 – 188Combined sources4
Helixi189 – 191Combined sources3
Turni192 – 195Combined sources4
Helixi197 – 255Combined sources59
Helixi257 – 259Combined sources3
Helixi263 – 275Combined sources13
Helixi279 – 290Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ABHX-ray2.00A/B1-305[»]
3ACOX-ray2.70A/B1-343[»]
3HAJX-ray2.78A/B1-486[»]
3Q0KX-ray2.60A/B/C/D16-304[»]
ProteinModelPortaliQ9UNF0
SMRiQ9UNF0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNF0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 282F-BARPROSITE-ProRule annotationAdd BLAST272
Domaini426 – 486SH3PROSITE-ProRule annotationAdd BLAST61

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 274Add BLAST250

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi362 – 364NPF13
Motifi405 – 407NPF23
Motifi417 – 419NPF33

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain.4 Publications

Sequence similaritiesi

Belongs to the PACSIN family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ9UNF0
KOiK20123
OMAiHHAACKE
OrthoDBiEOG091G0AS9
PhylomeDBiQ9UNF0
TreeFamiTF313677

Family and domain databases

CDDicd07679 F-BAR_PACSIN2, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.601 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR035743 PACSIN1/PACSIN2_SH3
IPR028521 PACSIN2
IPR037453 PACSIN2_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF14 PTHR23065:SF14, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCSD LMNCLHERAR
60 70 80 90 100
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAFMSEA ERVSELHLEV
110 120 130 140 150
KASLMNDDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK
160 170 180 190 200
EVEAAKKAHH AACKEEKLAI SREANSKADP SLNPEQLKKL QDKIEKCKQD
210 220 230 240 250
VLKTKEKYEK SLKELDQGTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE
260 270 280 290 300
VQKHLDLSNV AGYKAIYHDL EQSIRAADAV EDLRWFRANH GPGMAMNWPQ
310 320 330 340 350
FEEWSADLNR TLSRREKKKA TDGVTLTGIN QTGDQSLPSK PSSTLNVPSN
360 370 380 390 400
PAQSAQSQSS YNPFEDEDDT GSTVSEKDDT KAKNVSSYEK TQSYPTDWSD
410 420 430 440 450
DESNNPFSST DANGDSNPFD DDATSGTEVR VRALYDYEGQ EHDELSFKAG
460 470 480
DELTKMEDED EQGWCKGRLD NGQVGLYPAN YVEAIQ
Length:486
Mass (Da):55,739
Last modified:August 13, 2002 - v2
Checksum:i821DBEF65DAD1AA8
GO
Isoform 2 (identifier: Q9UNF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-384: Missing.

Show »
Length:445
Mass (Da):51,353
Checksum:i1DA87A2D42B5D5F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182L → F in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti256D → N in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti309N → I in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti336S → F in AAD41781 (PubMed:10431838).Curated1
Sequence conflicti378 – 380DDT → EDI in AAD41781 (PubMed:10431838).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053555175N → S. Corresponds to variant dbSNP:rs35383004Ensembl.1
Natural variantiVAR_013711294M → I. Corresponds to variant dbSNP:rs2746984Ensembl.1
Natural variantiVAR_013712324V → F. Corresponds to variant dbSNP:rs1062913Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004517344 – 384Missing in isoform 2. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128536 mRNA Translation: AAD41781.1
AL136845 mRNA Translation: CAB66779.1
CR456536 mRNA Translation: CAG30422.1
AL022476 Genomic DNA No translation available.
AL049758 Genomic DNA No translation available.
BC008037 mRNA Translation: AAH08037.1
AL389984 mRNA Translation: CAB97538.1
CCDSiCCDS43023.1 [Q9UNF0-1]
CCDS54536.1 [Q9UNF0-2]
RefSeqiNP_001171899.1, NM_001184970.1 [Q9UNF0-1]
NP_001171900.1, NM_001184971.1 [Q9UNF0-2]
NP_009160.2, NM_007229.3 [Q9UNF0-1]
XP_005261376.1, XM_005261319.3 [Q9UNF0-2]
XP_016884053.1, XM_017028564.1 [Q9UNF0-1]
UniGeneiHs.162877

Genome annotation databases

EnsembliENST00000263246; ENSP00000263246; ENSG00000100266 [Q9UNF0-1]
ENST00000337959; ENSP00000338379; ENSG00000100266 [Q9UNF0-2]
ENST00000402229; ENSP00000385040; ENSG00000100266 [Q9UNF0-1]
ENST00000403744; ENSP00000385372; ENSG00000100266 [Q9UNF0-1]
ENST00000407585; ENSP00000385952; ENSG00000100266 [Q9UNF0-2]
GeneIDi11252
KEGGihsa:11252
UCSCiuc003bdf.5 human [Q9UNF0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPACN2_HUMAN
AccessioniPrimary (citable) accession number: Q9UNF0
Secondary accession number(s): O95921
, Q96HV9, Q9H0D3, Q9NPN1, Q9Y4V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: March 28, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome