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Protein

Rab effector Noc2

Gene

RPH3AL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells (By similarity). Acts as a potential RAB3B effector protein in epithelial cells.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri89 – 14658FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • cytoskeletal protein binding Source: ProtInc
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rab effector Noc2
Alternative name(s):
No C2 domains protein
Rabphilin-3A-like protein
Gene namesi
Name:RPH3AL
Synonyms:NOC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:10296. RPH3AL.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • secretory granule membrane Source: Ensembl
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34658.

Polymorphism and mutation databases

BioMutaiRPH3AL.
DMDMi74735140.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Rab effector Noc2PRO_0000278263Add
BLAST

Proteomic databases

PaxDbiQ9UNE2.
PRIDEiQ9UNE2.

PTM databases

PhosphoSiteiQ9UNE2.

Expressioni

Tissue specificityi

Moderate to high levels of expression in thyroid, ovary, stomach, heart, pancreas, skeletal muscle, kidney and liver. Also detected in epithelial cells.2 Publications

Gene expression databases

BgeeiQ9UNE2.
CleanExiHS_RPH3AL.
ExpressionAtlasiQ9UNE2. baseline and differential.
GenevisibleiQ9UNE2. HS.

Organism-specific databases

HPAiHPA024311.

Interactioni

Subunit structurei

Recruited to dense-core vesicles through specific interaction with RAB27A in endocrine cells. Interacts with RAB3A, RAB3B, RAB3C and RAB3D. Interacts with ZYX (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MID2Q9UJV3-23EBI-2855824,EBI-10172526

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • cytoskeletal protein binding Source: ProtInc
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

BioGridi114880. 5 interactions.
IntActiQ9UNE2. 3 interactions.
STRINGi9606.ENSP00000328977.

Structurei

3D structure databases

ProteinModelPortaliQ9UNE2.
SMRiQ9UNE2. Positions 45-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 158118RabBDPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminus of the RabBD domain is necessary and sufficient for interaction with RAB27A.By similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri89 – 14658FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00440000034248.
HOGENOMiHOG000126816.
HOVERGENiHBG052990.
InParanoidiQ9UNE2.
KOiK19939.
OMAiEAPRMGF.
PhylomeDBiQ9UNE2.
TreeFamiTF342971.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010911. Rab_BD.
IPR030538. Rab_effect_Noc2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF244. PTHR10024:SF244. 1 hit.
PfamiPF02318. FYVE_2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNE2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTIFGSGN DQWVCPNDRQ LALRAKLQTG WSVHTYQTEK QRRKQHLSPA
60 70 80 90 100
EVEAILQVIQ RAERLDVLEQ QRIGRLVERL ETMRRNVMGN GLSQCLLCGE
110 120 130 140 150
VLGFLGSSSV FCKDCRKKVC TKCGIEASPG QKRPLWLCKI CSEQREVWKR
160 170 180 190 200
SGAWFYKGLP KYILPLKTPG RADDPHFRPL PTEPAEREPR SSETSRIYTW
210 220 230 240 250
ARGRVVSSDS DSDSDLSSSS LEDRLPSTGV RDRKGDKPWK ESGGSVEAPR
260 270 280 290 300
MGFTHPPGHL SGCQSSLASG ETGTGSADPP GGPRPGLTRR APVKDTPGRA
310
PAADAAPAGP SSCLG
Length:315
Mass (Da):34,464
Last modified:May 1, 2000 - v1
Checksum:iFDDB315E0BBC7C23
GO
Isoform 2 (identifier: Q9UNE2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-146: Missing.

Note: No experimental confirmation available.
Show »
Length:286
Mass (Da):31,190
Checksum:iE208E7BF9A361BC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851R → Q in AAH05153 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei118 – 14629Missing in isoform 2. 1 PublicationVSP_023244Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129812 mRNA. Translation: AAD45582.1.
AK000469 mRNA. Translation: BAA91186.1.
CH471108 Genomic DNA. Translation: EAW90670.1.
CH471108 Genomic DNA. Translation: EAW90671.1.
BC005153 mRNA. Translation: AAH05153.1.
BC093776 mRNA. Translation: AAH93776.1.
BC113413 mRNA. Translation: AAI13414.1.
CCDSiCCDS10994.1. [Q9UNE2-1]
CCDS54059.1. [Q9UNE2-2]
RefSeqiNP_001177340.1. NM_001190411.1. [Q9UNE2-1]
NP_001177341.1. NM_001190412.1. [Q9UNE2-2]
NP_001177342.1. NM_001190413.1. [Q9UNE2-2]
NP_008918.1. NM_006987.3. [Q9UNE2-1]
XP_011533669.1. XM_011535367.1. [Q9UNE2-1]
XP_011533670.1. XM_011535368.1. [Q9UNE2-2]
XP_011545302.1. XM_011547000.1. [Q9UNE2-1]
XP_011545304.1. XM_011547002.1. [Q9UNE2-2]
UniGeneiHs.651925.

Genome annotation databases

EnsembliENST00000323434; ENSP00000319210; ENSG00000181031. [Q9UNE2-2]
ENST00000331302; ENSP00000328977; ENSG00000181031. [Q9UNE2-1]
ENST00000536489; ENSP00000438224; ENSG00000181031. [Q9UNE2-2]
ENST00000608278; ENSP00000483035; ENSG00000262334. [Q9UNE2-1]
ENST00000608519; ENSP00000476394; ENSG00000262334. [Q9UNE2-2]
ENST00000618002; ENSP00000479485; ENSG00000181031. [Q9UNE2-1]
ENST00000632000; ENSP00000487689; ENSG00000262334. [Q9UNE2-2]
ENST00000632545; ENSP00000487851; ENSG00000262334. [Q9UNE2-2]
ENST00000632616; ENSP00000487649; ENSG00000262334. [Q9UNE2-1]
GeneIDi9501.
KEGGihsa:9501.
UCSCiuc002fre.3. human. [Q9UNE2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129812 mRNA. Translation: AAD45582.1.
AK000469 mRNA. Translation: BAA91186.1.
CH471108 Genomic DNA. Translation: EAW90670.1.
CH471108 Genomic DNA. Translation: EAW90671.1.
BC005153 mRNA. Translation: AAH05153.1.
BC093776 mRNA. Translation: AAH93776.1.
BC113413 mRNA. Translation: AAI13414.1.
CCDSiCCDS10994.1. [Q9UNE2-1]
CCDS54059.1. [Q9UNE2-2]
RefSeqiNP_001177340.1. NM_001190411.1. [Q9UNE2-1]
NP_001177341.1. NM_001190412.1. [Q9UNE2-2]
NP_001177342.1. NM_001190413.1. [Q9UNE2-2]
NP_008918.1. NM_006987.3. [Q9UNE2-1]
XP_011533669.1. XM_011535367.1. [Q9UNE2-1]
XP_011533670.1. XM_011535368.1. [Q9UNE2-2]
XP_011545302.1. XM_011547000.1. [Q9UNE2-1]
XP_011545304.1. XM_011547002.1. [Q9UNE2-2]
UniGeneiHs.651925.

3D structure databases

ProteinModelPortaliQ9UNE2.
SMRiQ9UNE2. Positions 45-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114880. 5 interactions.
IntActiQ9UNE2. 3 interactions.
STRINGi9606.ENSP00000328977.

PTM databases

PhosphoSiteiQ9UNE2.

Polymorphism and mutation databases

BioMutaiRPH3AL.
DMDMi74735140.

Proteomic databases

PaxDbiQ9UNE2.
PRIDEiQ9UNE2.

Protocols and materials databases

DNASUi9501.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323434; ENSP00000319210; ENSG00000181031. [Q9UNE2-2]
ENST00000331302; ENSP00000328977; ENSG00000181031. [Q9UNE2-1]
ENST00000536489; ENSP00000438224; ENSG00000181031. [Q9UNE2-2]
ENST00000608278; ENSP00000483035; ENSG00000262334. [Q9UNE2-1]
ENST00000608519; ENSP00000476394; ENSG00000262334. [Q9UNE2-2]
ENST00000618002; ENSP00000479485; ENSG00000181031. [Q9UNE2-1]
ENST00000632000; ENSP00000487689; ENSG00000262334. [Q9UNE2-2]
ENST00000632545; ENSP00000487851; ENSG00000262334. [Q9UNE2-2]
ENST00000632616; ENSP00000487649; ENSG00000262334. [Q9UNE2-1]
GeneIDi9501.
KEGGihsa:9501.
UCSCiuc002fre.3. human. [Q9UNE2-1]

Organism-specific databases

CTDi9501.
GeneCardsiRPH3AL.
HGNCiHGNC:10296. RPH3AL.
HPAiHPA024311.
MIMi604881. gene.
neXtProtiNX_Q9UNE2.
PharmGKBiPA34658.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00440000034248.
HOGENOMiHOG000126816.
HOVERGENiHBG052990.
InParanoidiQ9UNE2.
KOiK19939.
OMAiEAPRMGF.
PhylomeDBiQ9UNE2.
TreeFamiTF342971.

Miscellaneous databases

ChiTaRSiRPH3AL. human.
GeneWikiiRPH3AL.
GenomeRNAii9501.
NextBioi35602.
PROiQ9UNE2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UNE2.
CleanExiHS_RPH3AL.
ExpressionAtlasiQ9UNE2. baseline and differential.
GenevisibleiQ9UNE2. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010911. Rab_BD.
IPR030538. Rab_effect_Noc2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF244. PTHR10024:SF244. 1 hit.
PfamiPF02318. FYVE_2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a human ortholog (RPH3AL) of (RNO)Rph3al from a candidate 17p13.3 medulloblastoma tumor suppressor locus."
    Smith J.S., Tachibana I., Allen C., Chiappa S.A., Lee H.K., McIver B., Jenkins R.B., Raffel C.
    Genomics 59:97-101(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Muscle.
  5. "Identification and characterization of Noc2 as a potential Rab3B effector protein in epithelial cells."
    Manabe S., Nishimura N., Yamamoto Y., Kitamura H., Morimoto S., Imai M., Nagahiro S., Seino S., Sasaki T.
    Biochem. Biophys. Res. Commun. 316:218-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A RAB3B EFFECTOR, INTERACTION WITH RAB3B, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiRPH3L_HUMAN
AccessioniPrimary (citable) accession number: Q9UNE2
Secondary accession number(s): D3DTG7, Q9BSB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.