Reviewed, UniProtKB/Swiss-Prot Q9UNE2 (RPH3L_HUMAN)

Names and origin

Protein names

Recommended name:
    Rab effector Noc2
Alternative name(s):
    Rabphilin-3A-like protein
    No C2 domains protein

Gene names

Name:	RPH3AL
Synonyms:	NOC2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells By similarity. Acts as a potential RAB3B effector protein in epithelial cells.
Subunit structure	Recruited to dense-core vesicles through specific interaction with RAB27A in endocrine cells. Interacts with RAB3A, RAB3B, RAB3C and RAB3D. Interacts with ZYX By similarity.
Subcellular location	Cytoplasm. Cytoplasmic vesicle › secretory vesicle membrane. Note: Recruited to the vesicle membrane in a GTP- and RAB3B-dependent manner in epithelial cells. Ref.4
Tissue specificity	Moderate to high levels of expression in thyroid, ovary, stomach, heart, pancreas, skeletal muscle, kidney and liver. Also detected in epithelial cells. Ref.4 Ref.1
Domain	The N-terminus of the RabBD domain is necessary and sufficient for interaction with RAB27A By similarity.
Sequence similarities	Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain.

Ontologies

Keywords
Biological process	Exocytosis
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Gene Ontology (GO)
Biological process	exocytosis Traceable author statement. Source: ProtInc intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	secretory granule membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	Rab GTPase binding Inferred from electronic annotation. Source: InterPro cytoskeletal protein binding Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UNE2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UNE2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-146: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Rab effector Noc2		PRO_0000278263
Regions
	Domain	41 – 158	118	RabBD
	Zinc finger	89 – 146	58	FYVE-type
Natural variations
	Alternative sequence	118 – 146	29	Missing in isoform 2.		VSP_023244
Experimental info
	Sequence conflict	85	1	R → Q in AAH05153. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FDDB315E0BBC7C23	FASTA	315	34,464
	10 20 30 40 50 60 MADTIFGSGN DQWVCPNDRQ LALRAKLQTG WSVHTYQTEK QRRKQHLSPA EVEAILQVIQ 70 80 90 100 110 120 RAERLDVLEQ QRIGRLVERL ETMRRNVMGN GLSQCLLCGE VLGFLGSSSV FCKDCRKKVC 130 140 150 160 170 180 TKCGIEASPG QKRPLWLCKI CSEQREVWKR SGAWFYKGLP KYILPLKTPG RADDPHFRPL 190 200 210 220 230 240 PTEPAEREPR SSETSRIYTW ARGRVVSSDS DSDSDLSSSS LEDRLPSTGV RDRKGDKPWK 250 260 270 280 290 300 ESGGSVEAPR MGFTHPPGHL SGCQSSLASG ETGTGSADPP GGPRPGLTRR APVKDTPGRA 310 PAADAAPAGP SSCLG
	Isoform 2. Checksum: E208E7BF9A361BC3	FASTA	286	31,190
	10 20 30 40 50 60 MADTIFGSGN DQWVCPNDRQ LALRAKLQTG WSVHTYQTEK QRRKQHLSPA EVEAILQVIQ 70 80 90 100 110 120 RAERLDVLEQ QRIGRLVERL ETMRRNVMGN GLSQCLLCGE VLGFLGSSSV FCKDCRKVWK 130 140 150 160 170 180 RSGAWFYKGL PKYILPLKTP GRADDPHFRP LPTEPAEREP RSSETSRIYT WARGRVVSSD 190 200 210 220 230 240 SDSDSDLSSS SLEDRLPSTG VRDRKGDKPW KESGGSVEAP RMGFTHPPGH LSGCQSSLAS 250 260 270 280 GETGTGSADP PGGPRPGLTR RAPVKDTPGR APAADAAPAG PSSCLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human ortholog (RPH3AL) of (RNO)Rph3al from a candidate 17p13.3 medulloblastoma tumor suppressor locus." Smith J.S., Tachibana I., Allen C., Chiappa S.A., Lee H.K., McIver B., Jenkins R.B., Raffel C. Genomics 59:97-101(1999) [PubMed: 10395805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle.
[4]	"Identification and characterization of Noc2 as a potential Rab3B effector protein in epithelial cells." Manabe S., Nishimura N., Yamamoto Y., Kitamura H., Morimoto S., Imai M., Nagahiro S., Seino S., Sasaki T. Biochem. Biophys. Res. Commun. 316:218-225(2004) [PubMed: 15003533] [Abstract] Cited for: FUNCTION AS A RAB3B EFFECTOR, INTERACTION WITH RAB3B, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF129812 mRNA. Translation: AAD45582.1. AK000469 mRNA. Translation: BAA91186.1. BC005153 mRNA. Translation: AAH05153.1. BC093776 mRNA. Translation: AAH93776.1. BC113413 mRNA. Translation: AAI13414.1.
IPI	IPI00012958. IPI00186016.
RefSeq	NP_008918.1.
UniGene	Hs.651925
3D structure databases
HSSP	HSSP built from PDB template 1ZBD based on UniProtKB P47709.
ModBase	Search...
Proteomic databases
PRIDE	Q9UNE2.
Genome annotation databases
Ensembl	ENSG00000181031. Homo sapiens. [Contig view]
GeneID	9501.
KEGG	hsa:9501.
Organism-specific databases
GeneCards	GC17M000100.
HGNC	HGNC:10296. RPH3AL.
MIM	604881. gene.
PharmGKB	PA34658.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9UNE2.
OMA	Q9UNE2. QRKSQCL.
Gene expression databases
ArrayExpress	Q9UNE2.
Bgee	Q9UNE2.
CleanEx	HS_RPH3AL.
Family and domain databases
InterPro	IPR010911. Rab_bd_domain. IPR003315. Rabphilin3A_effector_Zn-bd. IPR017455. Znf_FYVE-rel. [Graphical view]
Pfam	PF02318. RPH3A_effector. 1 hit. [Graphical view]
PROSITE	PS50916. RABBD. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35602.
SOURCE	Search...

Entry information

Entry name

RPH3L_HUMAN

Accession

Primary (citable) accession number: Q9UNE2
Secondary accession number(s): Q9BSB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)