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Protein

DNA polymerase iota

Gene

POLI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.7 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Magnesium1
Binding sitei64dNTP1
Binding sitei96dNTP1
Metal bindingi151Magnesium1
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: ProtInc
  • DNA replication Source: UniProtKB-KW
  • error-prone translesion synthesis Source: Reactome
  • translesion synthesis Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Schiff base

Enzyme and pathway databases

BioCyciZFISH:HS02300-MONOMER.
BRENDAi2.7.7.7. 2681.
ReactomeiR-HSA-5656121. Translesion synthesis by POLI.
R-HSA-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase iota (EC:2.7.7.7)
Alternative name(s):
Eta2
RAD30 homolog B
Gene namesi
Name:POLI
Synonyms:RAD30B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:9182. POLI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11201.
OpenTargetsiENSG00000101751.
PharmGKBiPA33502.

Chemistry databases

ChEMBLiCHEMBL5391.

Polymorphism and mutation databases

BioMutaiPOLI.
DMDMi327478565.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739881 – 740DNA polymerase iotaAdd BLAST740

Proteomic databases

EPDiQ9UNA4.
MaxQBiQ9UNA4.
PaxDbiQ9UNA4.
PeptideAtlasiQ9UNA4.
PRIDEiQ9UNA4.

PTM databases

iPTMnetiQ9UNA4.
PhosphoSitePlusiQ9UNA4.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in testis.2 Publications

Gene expression databases

BgeeiENSG00000101751.
CleanExiHS_POLI.
ExpressionAtlasiQ9UNA4. baseline and differential.
GenevisibleiQ9UNA4. HS.

Organism-specific databases

HPAiHPA012000.

Interactioni

Subunit structurei

Interacts with REV1 (By similarity). Interacts with POLH.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DAZAP2Q150383EBI-741774,EBI-724310
PCNAQ6FI352EBI-741774,EBI-8469539
TMEM239Q8WW343EBI-741774,EBI-9675724
TRAF6Q9Y4K33EBI-741774,EBI-359276
TRIM39Q9HCM94EBI-741774,EBI-739510
UBCP0CG484EBI-741774,EBI-3390054
XRCC1P188872EBI-741774,EBI-947466
ZBTB44Q8NCP53EBI-741774,EBI-5658292

Protein-protein interaction databases

BioGridi116370. 16 interactors.
IntActiQ9UNA4. 17 interactors.
MINTiMINT-1189678.
STRINGi9606.ENSP00000462664.

Chemistry databases

BindingDBiQ9UNA4.

Structurei

Secondary structure

1740
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi55 – 60Combined sources6
Helixi63 – 71Combined sources9
Helixi73 – 75Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi80 – 84Combined sources5
Beta strandi87 – 91Combined sources5
Helixi93 – 96Combined sources4
Turni97 – 99Combined sources3
Beta strandi102 – 105Combined sources4
Helixi106 – 112Combined sources7
Beta strandi113 – 115Combined sources3
Beta strandi117 – 120Combined sources4
Helixi125 – 141Combined sources17
Beta strandi145 – 148Combined sources4
Turni149 – 151Combined sources3
Beta strandi152 – 156Combined sources5
Helixi158 – 167Combined sources10
Beta strandi170 – 172Combined sources3
Helixi173 – 175Combined sources3
Beta strandi180 – 182Combined sources3
Helixi183 – 185Combined sources3
Helixi193 – 216Combined sources24
Beta strandi220 – 227Combined sources8
Helixi228 – 235Combined sources8
Beta strandi236 – 238Combined sources3
Turni239 – 241Combined sources3
Beta strandi243 – 245Combined sources3
Helixi248 – 250Combined sources3
Helixi251 – 257Combined sources7
Helixi261 – 263Combined sources3
Helixi269 – 277Combined sources9
Helixi283 – 288Combined sources6
Helixi291 – 310Combined sources20
Beta strandi325 – 332Combined sources8
Turni334 – 336Combined sources3
Beta strandi339 – 341Combined sources3
Helixi342 – 360Combined sources19
Beta strandi362 – 374Combined sources13
Turni377 – 379Combined sources3
Beta strandi383 – 388Combined sources6
Turni391 – 393Combined sources3
Beta strandi397 – 399Combined sources3
Helixi401 – 404Combined sources4
Helixi405 – 419Combined sources15
Beta strandi422 – 424Combined sources3
Beta strandi427 – 439Combined sources13
Helixi529 – 534Combined sources6
Helixi537 – 544Combined sources8
Beta strandi704 – 707Combined sources4
Helixi710 – 713Combined sources4
Helixi718 – 731Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3NX-ray2.30A/B52-439[»]
1ZETX-ray2.30A52-439[»]
2ALZX-ray2.50A50-439[»]
2DPIX-ray2.30A26-445[»]
2DPJX-ray2.30A26-445[»]
2FLLX-ray2.60A26-445[»]
2FLNX-ray2.50A26-445[»]
2FLPX-ray2.40A26-445[»]
2KHUNMR-A697-740[»]
2KHWNMR-A697-740[»]
2KTFNMR-B704-730[»]
2L0FNMR-B699-740[»]
2L0GNMR-A704-730[»]
2MBBNMR-A516-555[»]
3EPGX-ray2.50A26-445[»]
3EPIX-ray2.90A26-445[»]
3G6VX-ray2.20A26-445[»]
3G6XX-ray2.08A26-445[»]
3G6YX-ray2.10A26-445[»]
3GV5X-ray2.00B/D26-445[»]
3GV7X-ray2.20B26-445[»]
3GV8X-ray2.00B26-445[»]
3H40X-ray2.30A51-439[»]
3H4BX-ray2.85A50-439[»]
3H4DX-ray2.20A50-439[»]
3NGDX-ray2.80A26-445[»]
3OSNX-ray1.90A26-445[»]
3Q8PX-ray1.95B26-445[»]
3Q8QX-ray2.03B26-445[»]
3Q8RX-ray2.45B26-445[»]
3Q8SX-ray2.09B26-445[»]
4EBCX-ray2.90A26-445[»]
4EBDX-ray2.57A26-445[»]
4EBEX-ray2.10A26-445[»]
4EYHX-ray2.90B26-445[»]
4EYIX-ray2.90B26-445[»]
4FS1X-ray2.50A26-445[»]
4FS2X-ray2.05A26-445[»]
5KT2X-ray2.49A26-445[»]
5KT3X-ray2.64A26-445[»]
5KT4X-ray2.78A1-445[»]
5KT5X-ray2.80A1-445[»]
5KT6X-ray3.54A1-445[»]
5KT7X-ray3.15A1-445[»]
ProteinModelPortaliQ9UNA4.
SMRiQ9UNA4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNA4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 268UmuCPROSITE-ProRule annotationAdd BLAST214

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 332DNA binding8
Regioni368 – 386DNA bindingAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi589 – 617Ser-richAdd BLAST29

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ9UNA4.
KOiK03510.
OMAiCFCNLKA.
OrthoDBiEOG091G033F.
PhylomeDBiQ9UNA4.
TreeFamiTF324222.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UNA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA
60 70 80 90 100
SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV
110 120 130 140 150
KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF
160 170 180 190 200
DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV
210 220 230 240 250
GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES
260 270 280 290 300
CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI
310 320 330 340 350
SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL
360 370 380 390 400
ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG
410 420 430 440 450
NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID
460 470 480 490 500
YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR
510 520 530 540 550
ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE
560 570 580 590 600
KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC
610 620 630 640 650
EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN
660 670 680 690 700
PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE
710 720 730 740
KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
Length:740
Mass (Da):83,006
Last modified:April 5, 2011 - v3
Checksum:iC1A5BF0894E91FDF
GO

Sequence cautioni

The sequence AAD50381 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF63383 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH32662 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAM11872 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB66605 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15Missing in AAD50381 (PubMed:10458907).Curated1
Sequence conflicti15Missing in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti15Missing in AAF63383 (Ref. 6) Curated1
Sequence conflicti337S → T in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti433V → A in AAF63383 (Ref. 6) Curated1
Sequence conflicti514D → G in AAF63383 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02123996R → G.1 PublicationCorresponds to variant rs3218778dbSNPEnsembl.1
Natural variantiVAR_021240261I → M.1 PublicationCorresponds to variant rs3218784dbSNPEnsembl.1
Natural variantiVAR_021241276E → K.1 PublicationCorresponds to variant rs3218783dbSNPEnsembl.1
Natural variantiVAR_021242474H → R.1 PublicationCorresponds to variant rs3730823dbSNPEnsembl.1
Natural variantiVAR_021243532F → S.2 PublicationsCorresponds to variant rs3218786dbSNPEnsembl.1
Natural variantiVAR_021244560C → R.1 PublicationCorresponds to variant rs3218787dbSNPEnsembl.1
Natural variantiVAR_021245731A → T.2 PublicationsCorresponds to variant rs8305dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY094607 Genomic DNA. Translation: AAM11872.1. Sequence problems.
AC093462 Genomic DNA. No translation available.
AF140501 mRNA. Translation: AAD50381.1. Different initiation.
AL136670 mRNA. Translation: CAB66605.1. Different initiation.
BC032662 mRNA. Translation: AAH32662.1. Different initiation.
AF245438 mRNA. Translation: AAF63383.1. Different initiation.
CCDSiCCDS11954.2.
RefSeqiNP_009126.2. NM_007195.2.
UniGeneiHs.438533.

Genome annotation databases

EnsembliENST00000579534; ENSP00000462664; ENSG00000101751.
GeneIDi11201.
KEGGihsa:11201.
UCSCiuc002lfj.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY094607 Genomic DNA. Translation: AAM11872.1. Sequence problems.
AC093462 Genomic DNA. No translation available.
AF140501 mRNA. Translation: AAD50381.1. Different initiation.
AL136670 mRNA. Translation: CAB66605.1. Different initiation.
BC032662 mRNA. Translation: AAH32662.1. Different initiation.
AF245438 mRNA. Translation: AAF63383.1. Different initiation.
CCDSiCCDS11954.2.
RefSeqiNP_009126.2. NM_007195.2.
UniGeneiHs.438533.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3NX-ray2.30A/B52-439[»]
1ZETX-ray2.30A52-439[»]
2ALZX-ray2.50A50-439[»]
2DPIX-ray2.30A26-445[»]
2DPJX-ray2.30A26-445[»]
2FLLX-ray2.60A26-445[»]
2FLNX-ray2.50A26-445[»]
2FLPX-ray2.40A26-445[»]
2KHUNMR-A697-740[»]
2KHWNMR-A697-740[»]
2KTFNMR-B704-730[»]
2L0FNMR-B699-740[»]
2L0GNMR-A704-730[»]
2MBBNMR-A516-555[»]
3EPGX-ray2.50A26-445[»]
3EPIX-ray2.90A26-445[»]
3G6VX-ray2.20A26-445[»]
3G6XX-ray2.08A26-445[»]
3G6YX-ray2.10A26-445[»]
3GV5X-ray2.00B/D26-445[»]
3GV7X-ray2.20B26-445[»]
3GV8X-ray2.00B26-445[»]
3H40X-ray2.30A51-439[»]
3H4BX-ray2.85A50-439[»]
3H4DX-ray2.20A50-439[»]
3NGDX-ray2.80A26-445[»]
3OSNX-ray1.90A26-445[»]
3Q8PX-ray1.95B26-445[»]
3Q8QX-ray2.03B26-445[»]
3Q8RX-ray2.45B26-445[»]
3Q8SX-ray2.09B26-445[»]
4EBCX-ray2.90A26-445[»]
4EBDX-ray2.57A26-445[»]
4EBEX-ray2.10A26-445[»]
4EYHX-ray2.90B26-445[»]
4EYIX-ray2.90B26-445[»]
4FS1X-ray2.50A26-445[»]
4FS2X-ray2.05A26-445[»]
5KT2X-ray2.49A26-445[»]
5KT3X-ray2.64A26-445[»]
5KT4X-ray2.78A1-445[»]
5KT5X-ray2.80A1-445[»]
5KT6X-ray3.54A1-445[»]
5KT7X-ray3.15A1-445[»]
ProteinModelPortaliQ9UNA4.
SMRiQ9UNA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116370. 16 interactors.
IntActiQ9UNA4. 17 interactors.
MINTiMINT-1189678.
STRINGi9606.ENSP00000462664.

Chemistry databases

BindingDBiQ9UNA4.
ChEMBLiCHEMBL5391.

PTM databases

iPTMnetiQ9UNA4.
PhosphoSitePlusiQ9UNA4.

Polymorphism and mutation databases

BioMutaiPOLI.
DMDMi327478565.

Proteomic databases

EPDiQ9UNA4.
MaxQBiQ9UNA4.
PaxDbiQ9UNA4.
PeptideAtlasiQ9UNA4.
PRIDEiQ9UNA4.

Protocols and materials databases

DNASUi11201.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000579534; ENSP00000462664; ENSG00000101751.
GeneIDi11201.
KEGGihsa:11201.
UCSCiuc002lfj.5. human.

Organism-specific databases

CTDi11201.
DisGeNETi11201.
GeneCardsiPOLI.
H-InvDBHIX0014457.
HGNCiHGNC:9182. POLI.
HPAiHPA012000.
MIMi605252. gene.
neXtProtiNX_Q9UNA4.
OpenTargetsiENSG00000101751.
PharmGKBiPA33502.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ9UNA4.
KOiK03510.
OMAiCFCNLKA.
OrthoDBiEOG091G033F.
PhylomeDBiQ9UNA4.
TreeFamiTF324222.

Enzyme and pathway databases

BioCyciZFISH:HS02300-MONOMER.
BRENDAi2.7.7.7. 2681.
ReactomeiR-HSA-5656121. Translesion synthesis by POLI.
R-HSA-5656169. Termination of translesion DNA synthesis.

Miscellaneous databases

ChiTaRSiPOLI. human.
EvolutionaryTraceiQ9UNA4.
GeneWikiiPOLI.
GenomeRNAii11201.
PROiQ9UNA4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101751.
CleanExiHS_POLI.
ExpressionAtlasiQ9UNA4. baseline and differential.
GenevisibleiQ9UNA4. HS.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLI_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA4
Secondary accession number(s): Q8N590, Q9H0S1, Q9NYH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.