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UniProtKB - Q9UNA4 (POLI_HUMAN)

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Protein

DNA polymerase iota

Gene

POLI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.7 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Magnesium1
Binding sitei64dNTP1
Binding sitei96dNTP1
Metal bindingi151Magnesium1
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • DNA repair Source: ProtInc
  • DNA replication Source: UniProtKB-KW
  • error-prone translesion synthesis Source: Reactome
  • translesion synthesis Source: Reactome
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandMagnesium, Metal-binding, Schiff base

Enzyme and pathway databases

BRENDAi2.7.7.7. 2681.
ReactomeiR-HSA-5656121. Translesion synthesis by POLI.
R-HSA-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase iota (EC:2.7.7.7)
Alternative name(s):
Eta2
RAD30 homolog B
Gene namesi
Name:POLI
Synonyms:RAD30B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000101751.10.
HGNCiHGNC:9182. POLI.
MIMi605252. gene.
neXtProtiNX_Q9UNA4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11201.
OpenTargetsiENSG00000101751.
PharmGKBiPA33502.

Chemistry databases

ChEMBLiCHEMBL5391.

Polymorphism and mutation databases

BioMutaiPOLI.
DMDMi327478565.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739881 – 740DNA polymerase iotaAdd BLAST740

Proteomic databases

EPDiQ9UNA4.
MaxQBiQ9UNA4.
PaxDbiQ9UNA4.
PeptideAtlasiQ9UNA4.
PRIDEiQ9UNA4.

PTM databases

iPTMnetiQ9UNA4.
PhosphoSitePlusiQ9UNA4.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in testis.2 Publications

Gene expression databases

BgeeiENSG00000101751.
CleanExiHS_POLI.
ExpressionAtlasiQ9UNA4. baseline and differential.
GenevisibleiQ9UNA4. HS.

Organism-specific databases

HPAiHPA012000.
HPA064696.

Interactioni

Subunit structurei

Interacts with REV1 (By similarity). Interacts with POLH.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116370. 18 interactors.
IntActiQ9UNA4. 17 interactors.
MINTiQ9UNA4.
STRINGi9606.ENSP00000462664.

Chemistry databases

BindingDBiQ9UNA4.

Structurei

Secondary structure

1740
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi55 – 60Combined sources6
Helixi63 – 71Combined sources9
Helixi73 – 75Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi80 – 84Combined sources5
Beta strandi87 – 91Combined sources5
Helixi93 – 96Combined sources4
Turni97 – 99Combined sources3
Beta strandi102 – 105Combined sources4
Helixi106 – 112Combined sources7
Beta strandi113 – 115Combined sources3
Beta strandi117 – 120Combined sources4
Helixi125 – 141Combined sources17
Beta strandi145 – 148Combined sources4
Turni149 – 151Combined sources3
Beta strandi152 – 156Combined sources5
Helixi158 – 167Combined sources10
Beta strandi170 – 172Combined sources3
Helixi173 – 175Combined sources3
Beta strandi180 – 182Combined sources3
Helixi183 – 185Combined sources3
Helixi193 – 216Combined sources24
Beta strandi220 – 227Combined sources8
Helixi228 – 235Combined sources8
Beta strandi236 – 238Combined sources3
Turni239 – 241Combined sources3
Beta strandi243 – 245Combined sources3
Helixi248 – 250Combined sources3
Helixi251 – 257Combined sources7
Helixi261 – 263Combined sources3
Helixi269 – 277Combined sources9
Helixi283 – 288Combined sources6
Helixi291 – 310Combined sources20
Beta strandi325 – 332Combined sources8
Turni334 – 336Combined sources3
Beta strandi339 – 341Combined sources3
Helixi342 – 360Combined sources19
Beta strandi362 – 374Combined sources13
Turni377 – 379Combined sources3
Beta strandi383 – 388Combined sources6
Turni391 – 393Combined sources3
Beta strandi397 – 399Combined sources3
Helixi401 – 404Combined sources4
Helixi405 – 419Combined sources15
Beta strandi422 – 424Combined sources3
Beta strandi427 – 439Combined sources13
Helixi529 – 534Combined sources6
Helixi537 – 544Combined sources8
Beta strandi704 – 707Combined sources4
Helixi710 – 713Combined sources4
Helixi718 – 731Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3NX-ray2.30A/B52-439[»]
1ZETX-ray2.30A52-439[»]
2ALZX-ray2.50A50-439[»]
2DPIX-ray2.30A26-445[»]
2DPJX-ray2.30A26-445[»]
2FLLX-ray2.60A26-445[»]
2FLNX-ray2.50A26-445[»]
2FLPX-ray2.40A26-445[»]
2KHUNMR-A697-740[»]
2KHWNMR-A697-740[»]
2KTFNMR-B704-730[»]
2L0FNMR-B699-740[»]
2L0GNMR-A704-730[»]
2MBBNMR-A516-555[»]
3EPGX-ray2.50A26-445[»]
3EPIX-ray2.90A26-445[»]
3G6VX-ray2.20A26-445[»]
3G6XX-ray2.08A26-445[»]
3G6YX-ray2.10A26-445[»]
3GV5X-ray2.00B/D26-445[»]
3GV7X-ray2.20B26-445[»]
3GV8X-ray2.00B26-445[»]
3H40X-ray2.30A51-439[»]
3H4BX-ray2.85A50-439[»]
3H4DX-ray2.20A50-439[»]
3NGDX-ray2.80A26-445[»]
3OSNX-ray1.90A26-445[»]
3Q8PX-ray1.95B26-445[»]
3Q8QX-ray2.03B26-445[»]
3Q8RX-ray2.45B26-445[»]
3Q8SX-ray2.09B26-445[»]
4EBCX-ray2.90A26-445[»]
4EBDX-ray2.57A26-445[»]
4EBEX-ray2.10A26-445[»]
4EYHX-ray2.90B26-445[»]
4EYIX-ray2.90B26-445[»]
4FS1X-ray2.50A26-445[»]
4FS2X-ray2.05A26-445[»]
5KT2X-ray2.49A26-445[»]
5KT3X-ray2.64A26-445[»]
5KT4X-ray2.78A1-445[»]
5KT5X-ray2.80A1-445[»]
5KT6X-ray3.54A1-445[»]
5KT7X-ray3.15A1-445[»]
5ULWX-ray2.62A26-445[»]
5ULXX-ray1.96A26-445[»]
ProteinModelPortaliQ9UNA4.
SMRiQ9UNA4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNA4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 268UmuCPROSITE-ProRule annotationAdd BLAST214

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 332DNA binding8
Regioni368 – 386DNA bindingAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi589 – 617Ser-richAdd BLAST29

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ9UNA4.
KOiK03510.
OMAiCFCNLKA.
OrthoDBiEOG091G033F.
PhylomeDBiQ9UNA4.
TreeFamiTF324222.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiView protein in InterPro
IPR017061. DNA_pol_eta/kappa/iota/IV.
IPR036775. DNA_pol_Y-fam_lit_finger_sf.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
PfamiView protein in Pfam
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PIRSFiPIRSF036603. DPol_eta. 1 hit.
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiView protein in PROSITE
PS50173. UMUC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UNA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA 
        60         70         80         90        100
SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV 
       110        120        130        140        150
KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF 
       160        170        180        190        200
DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV 
       210        220        230        240        250
GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES 
       260        270        280        290        300
CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI 
       310        320        330        340        350
SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL 
       360        370        380        390        400
ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG 
       410        420        430        440        450
NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID 
       460        470        480        490        500
YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR 
       510        520        530        540        550
ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE 
       560        570        580        590        600
KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC 
       610        620        630        640        650
EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN 
       660        670        680        690        700
PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE 
       710        720        730        740
KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
Length:740
Mass (Da):83,006
Last modified:April 5, 2011 - v3
Checksum:iC1A5BF0894E91FDF
GO

Sequence cautioni

The sequence AAD50381 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF63383 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH32662 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAM11872 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB66605 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15Missing in AAD50381 (PubMed:10458907).Curated1
Sequence conflicti15Missing in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti15Missing in AAF63383 (Ref. 6) Curated1
Sequence conflicti337S → T in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti433V → A in AAF63383 (Ref. 6) Curated1
Sequence conflicti514D → G in AAF63383 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02123996R → G1 PublicationCorresponds to variant dbSNP:rs3218778Ensembl.1
Natural variantiVAR_021240261I → M1 PublicationCorresponds to variant dbSNP:rs3218784Ensembl.1
Natural variantiVAR_021241276E → K1 PublicationCorresponds to variant dbSNP:rs3218783Ensembl.1
Natural variantiVAR_021242474H → R1 PublicationCorresponds to variant dbSNP:rs3730823Ensembl.1
Natural variantiVAR_021243532F → S2 PublicationsCorresponds to variant dbSNP:rs3218786Ensembl.1
Natural variantiVAR_021244560C → R1 PublicationCorresponds to variant dbSNP:rs3218787Ensembl.1
Natural variantiVAR_021245731A → T2 PublicationsCorresponds to variant dbSNP:rs8305Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY094607 Genomic DNA. Translation: AAM11872.1. Sequence problems.
AC093462 Genomic DNA. No translation available.
AF140501 mRNA. Translation: AAD50381.1. Different initiation.
AL136670 mRNA. Translation: CAB66605.1. Different initiation.
BC032662 mRNA. Translation: AAH32662.1. Different initiation.
AF245438 mRNA. Translation: AAF63383.1. Different initiation.
CCDSiCCDS11954.2.
RefSeqiNP_009126.2. NM_007195.2.
UniGeneiHs.438533.

Genome annotation databases

EnsembliENST00000579534; ENSP00000462664; ENSG00000101751.
GeneIDi11201.
KEGGihsa:11201.
UCSCiuc002lfj.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPOLI_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA4
Secondary accession number(s): Q8N590, Q9H0S1, Q9NYH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: April 5, 2011
Last modified: February 28, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome