UniProtKB - Q9UNA4 (POLI_HUMAN)
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Protein
DNA polymerase iota
Gene
POLI
Organism
Homo sapiens (Human)
Status
Functioni
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.7 Publications
Catalytic activityi
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 59 | Magnesium | 1 | |
Binding sitei | 64 | dNTP | 1 | |
Binding sitei | 96 | dNTP | 1 | |
Metal bindingi | 151 | Magnesium | 1 | |
Active sitei | 152 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: Reactome
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA repair Source: ProtInc
- DNA replication Source: UniProtKB-KW
- error-prone translesion synthesis Source: Reactome
- translesion synthesis Source: Reactome
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication, DNA synthesis |
Ligand | Magnesium, Metal-binding, Schiff base |
Enzyme and pathway databases
BRENDAi | 2.7.7.7. 2681. |
Reactomei | R-HSA-5656121. Translesion synthesis by POLI. R-HSA-5656169. Termination of translesion DNA synthesis. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:POLI Synonyms:RAD30B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000101751.10. |
HGNCi | HGNC:9182. POLI. |
MIMi | 605252. gene. |
neXtProti | NX_Q9UNA4. |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 11201. |
OpenTargetsi | ENSG00000101751. |
PharmGKBi | PA33502. |
Chemistry databases
ChEMBLi | CHEMBL5391. |
Polymorphism and mutation databases
BioMutai | POLI. |
DMDMi | 327478565. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173988 | 1 – 740 | DNA polymerase iotaAdd BLAST | 740 |
Proteomic databases
EPDi | Q9UNA4. |
MaxQBi | Q9UNA4. |
PaxDbi | Q9UNA4. |
PeptideAtlasi | Q9UNA4. |
PRIDEi | Q9UNA4. |
PTM databases
iPTMneti | Q9UNA4. |
PhosphoSitePlusi | Q9UNA4. |
Expressioni
Tissue specificityi
Ubiquitous. Highly expressed in testis.2 Publications
Gene expression databases
Bgeei | ENSG00000101751. |
CleanExi | HS_POLI. |
ExpressionAtlasi | Q9UNA4. baseline and differential. |
Genevisiblei | Q9UNA4. HS. |
Organism-specific databases
HPAi | HPA012000. HPA064696. |
Interactioni
Subunit structurei
Interacts with REV1 (By similarity). Interacts with POLH.By similarity2 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 116370. 18 interactors. |
IntActi | Q9UNA4. 17 interactors. |
MINTi | Q9UNA4. |
STRINGi | 9606.ENSP00000462664. |
Chemistry databases
BindingDBi | Q9UNA4. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 55 – 60 | Combined sources | 6 | |
Helixi | 63 – 71 | Combined sources | 9 | |
Helixi | 73 – 75 | Combined sources | 3 | |
Beta strandi | 76 – 78 | Combined sources | 3 | |
Beta strandi | 80 – 84 | Combined sources | 5 | |
Beta strandi | 87 – 91 | Combined sources | 5 | |
Helixi | 93 – 96 | Combined sources | 4 | |
Turni | 97 – 99 | Combined sources | 3 | |
Beta strandi | 102 – 105 | Combined sources | 4 | |
Helixi | 106 – 112 | Combined sources | 7 | |
Beta strandi | 113 – 115 | Combined sources | 3 | |
Beta strandi | 117 – 120 | Combined sources | 4 | |
Helixi | 125 – 141 | Combined sources | 17 | |
Beta strandi | 145 – 148 | Combined sources | 4 | |
Turni | 149 – 151 | Combined sources | 3 | |
Beta strandi | 152 – 156 | Combined sources | 5 | |
Helixi | 158 – 167 | Combined sources | 10 | |
Beta strandi | 170 – 172 | Combined sources | 3 | |
Helixi | 173 – 175 | Combined sources | 3 | |
Beta strandi | 180 – 182 | Combined sources | 3 | |
Helixi | 183 – 185 | Combined sources | 3 | |
Helixi | 193 – 216 | Combined sources | 24 | |
Beta strandi | 220 – 227 | Combined sources | 8 | |
Helixi | 228 – 235 | Combined sources | 8 | |
Beta strandi | 236 – 238 | Combined sources | 3 | |
Turni | 239 – 241 | Combined sources | 3 | |
Beta strandi | 243 – 245 | Combined sources | 3 | |
Helixi | 248 – 250 | Combined sources | 3 | |
Helixi | 251 – 257 | Combined sources | 7 | |
Helixi | 261 – 263 | Combined sources | 3 | |
Helixi | 269 – 277 | Combined sources | 9 | |
Helixi | 283 – 288 | Combined sources | 6 | |
Helixi | 291 – 310 | Combined sources | 20 | |
Beta strandi | 325 – 332 | Combined sources | 8 | |
Turni | 334 – 336 | Combined sources | 3 | |
Beta strandi | 339 – 341 | Combined sources | 3 | |
Helixi | 342 – 360 | Combined sources | 19 | |
Beta strandi | 362 – 374 | Combined sources | 13 | |
Turni | 377 – 379 | Combined sources | 3 | |
Beta strandi | 383 – 388 | Combined sources | 6 | |
Turni | 391 – 393 | Combined sources | 3 | |
Beta strandi | 397 – 399 | Combined sources | 3 | |
Helixi | 401 – 404 | Combined sources | 4 | |
Helixi | 405 – 419 | Combined sources | 15 | |
Beta strandi | 422 – 424 | Combined sources | 3 | |
Beta strandi | 427 – 439 | Combined sources | 13 | |
Helixi | 529 – 534 | Combined sources | 6 | |
Helixi | 537 – 544 | Combined sources | 8 | |
Beta strandi | 704 – 707 | Combined sources | 4 | |
Helixi | 710 – 713 | Combined sources | 4 | |
Helixi | 718 – 731 | Combined sources | 14 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T3N | X-ray | 2.30 | A/B | 52-439 | [»] | |
1ZET | X-ray | 2.30 | A | 52-439 | [»] | |
2ALZ | X-ray | 2.50 | A | 50-439 | [»] | |
2DPI | X-ray | 2.30 | A | 26-445 | [»] | |
2DPJ | X-ray | 2.30 | A | 26-445 | [»] | |
2FLL | X-ray | 2.60 | A | 26-445 | [»] | |
2FLN | X-ray | 2.50 | A | 26-445 | [»] | |
2FLP | X-ray | 2.40 | A | 26-445 | [»] | |
2KHU | NMR | - | A | 697-740 | [»] | |
2KHW | NMR | - | A | 697-740 | [»] | |
2KTF | NMR | - | B | 704-730 | [»] | |
2L0F | NMR | - | B | 699-740 | [»] | |
2L0G | NMR | - | A | 704-730 | [»] | |
2MBB | NMR | - | A | 516-555 | [»] | |
3EPG | X-ray | 2.50 | A | 26-445 | [»] | |
3EPI | X-ray | 2.90 | A | 26-445 | [»] | |
3G6V | X-ray | 2.20 | A | 26-445 | [»] | |
3G6X | X-ray | 2.08 | A | 26-445 | [»] | |
3G6Y | X-ray | 2.10 | A | 26-445 | [»] | |
3GV5 | X-ray | 2.00 | B/D | 26-445 | [»] | |
3GV7 | X-ray | 2.20 | B | 26-445 | [»] | |
3GV8 | X-ray | 2.00 | B | 26-445 | [»] | |
3H40 | X-ray | 2.30 | A | 51-439 | [»] | |
3H4B | X-ray | 2.85 | A | 50-439 | [»] | |
3H4D | X-ray | 2.20 | A | 50-439 | [»] | |
3NGD | X-ray | 2.80 | A | 26-445 | [»] | |
3OSN | X-ray | 1.90 | A | 26-445 | [»] | |
3Q8P | X-ray | 1.95 | B | 26-445 | [»] | |
3Q8Q | X-ray | 2.03 | B | 26-445 | [»] | |
3Q8R | X-ray | 2.45 | B | 26-445 | [»] | |
3Q8S | X-ray | 2.09 | B | 26-445 | [»] | |
4EBC | X-ray | 2.90 | A | 26-445 | [»] | |
4EBD | X-ray | 2.57 | A | 26-445 | [»] | |
4EBE | X-ray | 2.10 | A | 26-445 | [»] | |
4EYH | X-ray | 2.90 | B | 26-445 | [»] | |
4EYI | X-ray | 2.90 | B | 26-445 | [»] | |
4FS1 | X-ray | 2.50 | A | 26-445 | [»] | |
4FS2 | X-ray | 2.05 | A | 26-445 | [»] | |
5KT2 | X-ray | 2.49 | A | 26-445 | [»] | |
5KT3 | X-ray | 2.64 | A | 26-445 | [»] | |
5KT4 | X-ray | 2.78 | A | 1-445 | [»] | |
5KT5 | X-ray | 2.80 | A | 1-445 | [»] | |
5KT6 | X-ray | 3.54 | A | 1-445 | [»] | |
5KT7 | X-ray | 3.15 | A | 1-445 | [»] | |
5ULW | X-ray | 2.62 | A | 26-445 | [»] | |
5ULX | X-ray | 1.96 | A | 26-445 | [»] | |
ProteinModelPortali | Q9UNA4. | |||||
SMRi | Q9UNA4. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UNA4. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 268 | UmuCPROSITE-ProRule annotationAdd BLAST | 214 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 325 – 332 | DNA binding | 8 | |
Regioni | 368 – 386 | DNA bindingAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 589 – 617 | Ser-richAdd BLAST | 29 |
Domaini
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
Sequence similaritiesi
Belongs to the DNA polymerase type-Y family.Curated
Phylogenomic databases
eggNOGi | KOG2095. Eukaryota. COG0389. LUCA. |
GeneTreei | ENSGT00530000062942. |
HOGENOMi | HOG000234325. |
HOVERGENi | HBG053634. |
InParanoidi | Q9UNA4. |
KOi | K03510. |
OMAi | CFCNLKA. |
OrthoDBi | EOG091G033F. |
PhylomeDBi | Q9UNA4. |
TreeFami | TF324222. |
Family and domain databases
Gene3Di | 3.30.1490.100. 1 hit. |
InterProi | View protein in InterPro IPR017061. DNA_pol_eta/kappa/iota/IV. IPR036775. DNA_pol_Y-fam_lit_finger_sf. IPR017961. DNA_pol_Y-fam_little_finger. IPR001126. UmuC. |
Pfami | View protein in Pfam PF00817. IMS. 1 hit. PF11799. IMS_C. 1 hit. |
PIRSFi | PIRSF036603. DPol_eta. 1 hit. |
SUPFAMi | SSF100879. SSF100879. 1 hit. |
PROSITEi | View protein in PROSITE PS50173. UMUC. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q9UNA4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA
60 70 80 90 100
SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV
110 120 130 140 150
KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF
160 170 180 190 200
DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV
210 220 230 240 250
GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES
260 270 280 290 300
CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI
310 320 330 340 350
SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL
360 370 380 390 400
ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG
410 420 430 440 450
NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID
460 470 480 490 500
YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR
510 520 530 540 550
ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE
560 570 580 590 600
KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC
610 620 630 640 650
EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN
660 670 680 690 700
PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE
710 720 730 740
KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
Sequence cautioni
The sequence AAD50381 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF63383 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH32662 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAM11872 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB66605 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | Missing in AAD50381 (PubMed:10458907).Curated | 1 | |
Sequence conflicti | 15 | Missing in CAB66605 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 15 | Missing in AAF63383 (Ref. 6) Curated | 1 | |
Sequence conflicti | 337 | S → T in CAB66605 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 433 | V → A in AAF63383 (Ref. 6) Curated | 1 | |
Sequence conflicti | 514 | D → G in AAF63383 (Ref. 6) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021239 | 96 | R → G1 PublicationCorresponds to variant dbSNP:rs3218778Ensembl. | 1 | |
Natural variantiVAR_021240 | 261 | I → M1 PublicationCorresponds to variant dbSNP:rs3218784Ensembl. | 1 | |
Natural variantiVAR_021241 | 276 | E → K1 PublicationCorresponds to variant dbSNP:rs3218783Ensembl. | 1 | |
Natural variantiVAR_021242 | 474 | H → R1 PublicationCorresponds to variant dbSNP:rs3730823Ensembl. | 1 | |
Natural variantiVAR_021243 | 532 | F → S2 PublicationsCorresponds to variant dbSNP:rs3218786Ensembl. | 1 | |
Natural variantiVAR_021244 | 560 | C → R1 PublicationCorresponds to variant dbSNP:rs3218787Ensembl. | 1 | |
Natural variantiVAR_021245 | 731 | A → T2 PublicationsCorresponds to variant dbSNP:rs8305Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY094607 Genomic DNA. Translation: AAM11872.1. Sequence problems. AC093462 Genomic DNA. No translation available. AF140501 mRNA. Translation: AAD50381.1. Different initiation. AL136670 mRNA. Translation: CAB66605.1. Different initiation. BC032662 mRNA. Translation: AAH32662.1. Different initiation. AF245438 mRNA. Translation: AAF63383.1. Different initiation. |
CCDSi | CCDS11954.2. |
RefSeqi | NP_009126.2. NM_007195.2. |
UniGenei | Hs.438533. |
Genome annotation databases
Ensembli | ENST00000579534; ENSP00000462664; ENSG00000101751. |
GeneIDi | 11201. |
KEGGi | hsa:11201. |
UCSCi | uc002lfj.5. human. |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Entry informationi
Entry namei | POLI_HUMAN | |
Accessioni | Q9UNA4Primary (citable) accession number: Q9UNA4 Secondary accession number(s): Q8N590, Q9H0S1, Q9NYH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 15, 2005 |
Last sequence update: | April 5, 2011 | |
Last modified: | February 28, 2018 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |