ID   A4GCT_HUMAN             Reviewed;         340 AA.
AC   Q9UNA3; Q0VDK1; Q0VDK2;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Alpha-1,4-N-acetylglucosaminyltransferase;
DE            Short=Alpha4GnT;
DE            EC=2.4.1.- {ECO:0000269|PubMed:10430883};
GN   Name=A4GNT;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND
RP   TISSUE SPECIFICITY.
RC   TISSUE=Stomach;
RX   PubMed=10430883; DOI=10.1073/pnas.96.16.8991;
RA   Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M.;
RT   "Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase
RT   that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in
RT   the gastric gland mucous cell-type mucin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ASP-218.
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to
CC       core 2 branched O-glycans (PubMed:10430883). Necessary for the
CC       synthesis of type III mucin which is specifically produced in the
CC       stomach, duodenum, and pancreatic duct (PubMed:10430883). May protect
CC       against inflammation-associated gastric adenocarcinomas (By
CC       similarity). {ECO:0000250|UniProtKB:Q14BT6,
CC       ECO:0000269|PubMed:10430883}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000305|PubMed:10430883}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Detected in stomach and pancreas.
CC       {ECO:0000269|PubMed:10430883}.
CC   -!- DOMAIN: The conserved DXD motif is involved in enzyme activity.
CC       {ECO:0000250|UniProtKB:Q9JI93}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 32 family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Functional Glycomics Gateway - GTase;
CC       Note=Alpha-1,4-N-acetylglucosaminyltransferase;
CC       URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_532";
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DR   EMBL; AF141315; AAD48406.1; -; mRNA.
DR   EMBL; BC119639; AAI19640.1; -; mRNA.
DR   EMBL; BC119640; AAI19641.1; -; mRNA.
DR   CCDS; CCDS3097.1; -.
DR   RefSeq; NP_057245.1; NM_016161.2.
DR   RefSeq; XP_016862032.1; XM_017006543.1.
DR   RefSeq; XP_016862033.1; XM_017006544.1.
DR   AlphaFoldDB; Q9UNA3; -.
DR   SMR; Q9UNA3; -.
DR   BioGRID; 119330; 59.
DR   IntAct; Q9UNA3; 11.
DR   STRING; 9606.ENSP00000236709; -.
DR   CAZy; GT32; Glycosyltransferase Family 32.
DR   GlyCosmos; Q9UNA3; 4 sites, No reported glycans.
DR   GlyGen; Q9UNA3; 4 sites.
DR   PhosphoSitePlus; Q9UNA3; -.
DR   BioMuta; A4GNT; -.
DR   DMDM; 25452797; -.
DR   MassIVE; Q9UNA3; -.
DR   PaxDb; 9606-ENSP00000236709; -.
DR   PeptideAtlas; Q9UNA3; -.
DR   Antibodypedia; 2373; 396 antibodies from 35 providers.
DR   DNASU; 51146; -.
DR   Ensembl; ENST00000236709.4; ENSP00000236709.3; ENSG00000118017.4.
DR   GeneID; 51146; -.
DR   KEGG; hsa:51146; -.
DR   MANE-Select; ENST00000236709.4; ENSP00000236709.3; NM_016161.3; NP_057245.1.
DR   UCSC; uc003ers.2; human.
DR   AGR; HGNC:17968; -.
DR   CTD; 51146; -.
DR   DisGeNET; 51146; -.
DR   GeneCards; A4GNT; -.
DR   HGNC; HGNC:17968; A4GNT.
DR   HPA; ENSG00000118017; Tissue enriched (stomach).
DR   neXtProt; NX_Q9UNA3; -.
DR   OpenTargets; ENSG00000118017; -.
DR   PharmGKB; PA134960042; -.
DR   VEuPathDB; HostDB:ENSG00000118017; -.
DR   eggNOG; KOG1928; Eukaryota.
DR   GeneTree; ENSGT00510000047981; -.
DR   HOGENOM; CLU_049512_2_0_1; -.
DR   InParanoid; Q9UNA3; -.
DR   OMA; IWDCMEN; -.
DR   OrthoDB; 2235518at2759; -.
DR   PhylomeDB; Q9UNA3; -.
DR   TreeFam; TF324053; -.
DR   PathwayCommons; Q9UNA3; -.
DR   Reactome; R-HSA-913709; O-linked glycosylation of mucins.
DR   SignaLink; Q9UNA3; -.
DR   UniPathway; UPA00378; -.
DR   BioGRID-ORCS; 51146; 6 hits in 1138 CRISPR screens.
DR   ChiTaRS; A4GNT; human.
DR   GenomeRNAi; 51146; -.
DR   Pharos; Q9UNA3; Tbio.
DR   PRO; PR:Q9UNA3; -.
DR   Proteomes; UP000005640; Chromosome 3.
DR   RNAct; Q9UNA3; Protein.
DR   Bgee; ENSG00000118017; Expressed in pylorus and 70 other cell types or tissues.
DR   GO; GO:0000139; C:Golgi membrane; TAS:Reactome.
DR   GO; GO:0016020; C:membrane; TAS:ProtInc.
DR   GO; GO:0008375; F:acetylglucosaminyltransferase activity; IDA:MGI.
DR   GO; GO:0005975; P:carbohydrate metabolic process; TAS:ProtInc.
DR   GO; GO:0050673; P:epithelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0009101; P:glycoprotein biosynthetic process; IDA:MGI.
DR   GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0016266; P:O-glycan processing; TAS:Reactome.
DR   GO; GO:0006493; P:protein O-linked glycosylation; IBA:GO_Central.
DR   Gene3D; 3.90.550.20; -; 1.
DR   InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR   InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR12042:SF22; ALPHA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE; 1.
DR   PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR   Pfam; PF04572; Gb3_synth; 1.
DR   Pfam; PF04488; Gly_transf_sug; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR   Genevisible; Q9UNA3; HS.
PE   1: Evidence at protein level;
KW   Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..340
FT                   /note="Alpha-1,4-N-acetylglucosaminyltransferase"
FT                   /id="PRO_0000080583"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..340
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           167..169
FT                   /note="DXD motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JI93"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        138
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        251
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        282
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         218
FT                   /note="A -> D (in dbSNP:rs2246945)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_022096"
SQ   SEQUENCE   340 AA;  39497 MW;  00D667A8394300AB CRC64;
     MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER
     MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD
     MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN
     FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED
     FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV
     IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK
//