Q9UNA3 (A4GCT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,4-N-acetylglucosaminyltransferase
Short name=Alpha4GnT
EC=2.4.1.-

Gene names

Name:

A4GNT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Detected in stomach and pancreas.
Domain	The conserved DXD motif is involved in enzyme activity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 32 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	acetylglucosaminyltransferase activity Inferred from direct assay Ref.1. Source: MGI galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Alpha-1,4-N-acetylglucosaminyltransferase		PRO_0000080583
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 25	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 340	315	Lumenal Potential
	Motif	167 – 169	3	DXD motif By similarity
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	218	1	A → D. Ref.2 Corresponds to variant rs2246945 [ dbSNP | Ensembl ].		VAR_022096

Sequences

	Sequence		Length	Mass (Da)
	Q9UNA3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 00D667A8394300AB	FASTA	340	39,497
	10 20 30 40 50 60 MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER 70 80 90 100 110 120 MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD 130 140 150 160 170 180 MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN 190 200 210 220 230 240 FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED 250 260 270 280 290 300 FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV 310 320 330 340 IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin." Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Stomach.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-218.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Alpha-1,4-N-acetylglucosaminyltransferase

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF141315 mRNA. Translation: AAD48406.1. BC119639 mRNA. Translation: AAI19640.1. BC119640 mRNA. Translation: AAI19641.1.
IPI	IPI00007712.
RefSeq	NP_057245.1. NM_016161.2.
UniGene	Hs.278960.
3D structure databases
ProteinModelPortal	Q9UNA3.
SMR	Q9UNA3. Positions 127-173.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000236709.
Protein family/group databases
CAZy	GT32. Glycosyltransferase Family 32.
Polymorphism databases
DMDM	25452797.
Proteomic databases
PaxDb	Q9UNA3.
PRIDE	Q9UNA3.
Protocols and materials databases
DNASU	51146.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000236709; ENSP00000236709; ENSG00000118017.
GeneID	51146.
KEGG	hsa:51146.
UCSC	uc003ers.2. human.
Organism-specific databases
CTD	51146.
GeneCards	GC03M137842.
HGNC	HGNC:17968. A4GNT.
HPA	HPA008017.
neXtProt	NX_Q9UNA3.
PharmGKB	PA134960042.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG251623.
HOGENOM	HOG000254772.
HOVERGEN	HBG106704.
InParanoid	Q9UNA3.
KO	K08251.
OMA	ESAAKIY.
OrthoDB	EOG4TTGJ8.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q9UNA3.
CleanEx	HS_A4GNT.
Genevestigator	Q9UNA3.
GermOnline	ENSG00000118017. Homo sapiens.
Family and domain databases
InterPro	IPR007652. A1-4-GlycosylTfrase_dom. IPR007577. GlycoTrfase_DXD_sugar-bd_CS. [Graphical view]
Pfam	PF04572. Gb3_synth. 1 hit. PF04488. Gly_transf_sug. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51146.
NextBio	54017.

Entry information

Entry name

A4GCT_HUMAN

Accession

Primary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.