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Protein

Alpha-1,4-N-acetylglucosaminyltransferase

Gene

A4GNT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans (PubMed:10430883). Necessary for the synthesis of type III mucin which is specifically produced in the stomach, duodenum, and pancreatic duct (PubMed:10430883). May protect against inflammation-associated gastric adenocarcinomas (By similarity).By similarity1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • glycoprotein biosynthetic process Source: MGI
  • negative regulation of epithelial cell proliferation Source: Ensembl
  • O-glycan processing Source: Reactome
  • protein O-linked glycosylation Source: ProtInc

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,4-N-acetylglucosaminyltransferase (EC:2.4.1.-1 Publication)
Short name:
Alpha4GnT
Gene namesi
Name:A4GNT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000118017.3.
HGNCiHGNC:17968. A4GNT.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 340LumenalSequence analysisAdd BLAST315

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi51146.
OpenTargetsiENSG00000118017.
PharmGKBiPA134960042.

Polymorphism and mutation databases

BioMutaiA4GNT.
DMDMi25452797.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805831 – 340Alpha-1,4-N-acetylglucosaminyltransferaseAdd BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9UNA3.
PRIDEiQ9UNA3.

PTM databases

PhosphoSitePlusiQ9UNA3.

Expressioni

Tissue specificityi

Detected in stomach and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000118017.
CleanExiHS_A4GNT.
GenevisibleiQ9UNA3. HS.

Organism-specific databases

HPAiHPA008017.

Interactioni

Protein-protein interaction databases

BioGridi119330. 21 interactors.
STRINGi9606.ENSP00000236709.

Structurei

3D structure databases

ProteinModelPortaliQ9UNA3.
SMRiQ9UNA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 169DXD motifBy similarity3

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
InParanoidiQ9UNA3.
KOiK08251.
OMAiDTEPSFN.
OrthoDBiEOG091G08S2.
PhylomeDBiQ9UNA3.
TreeFamiTF324053.

Family and domain databases

InterProiView protein in InterPro
IPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UNA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR
60 70 80 90 100
GIVFLETSER MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS
110 120 130 140 150
TYPAFSFLSA IDNVFLFPLD MKRLLEDTPL FSWYNQINAS AERNWLHISS
160 170 180 190 200
DASRLAIIWK YGGIYMDTDV ISIRPIPEEN FLAAQASRYS SNGIFGFLPH
210 220 230 240 250
HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED FQEVSDLRCL
260 270 280 290 300
NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV
310 320 330 340
IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK
Length:340
Mass (Da):39,497
Last modified:May 1, 2000 - v1
Checksum:i00D667A8394300AB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022096218A → D1 PublicationCorresponds to variant dbSNP:rs2246945Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSiCCDS3097.1.
RefSeqiNP_057245.1. NM_016161.2.
XP_016862032.1. XM_017006543.1.
XP_016862033.1. XM_017006544.1.
UniGeneiHs.278960.

Genome annotation databases

EnsembliENST00000236709; ENSP00000236709; ENSG00000118017.
GeneIDi51146.
KEGGihsa:51146.
UCSCiuc003ers.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiA4GCT_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families