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Protein

Alpha-1,4-N-acetylglucosaminyltransferase

Gene

A4GNT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • glycoprotein biosynthetic process Source: MGI
  • negative regulation of epithelial cell proliferation Source: Ensembl
  • O-glycan processing Source: Reactome
  • protein O-linked glycosylation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciZFISH:HS04186-MONOMER.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,4-N-acetylglucosaminyltransferase (EC:2.4.1.-)
Short name:
Alpha4GnT
Gene namesi
Name:A4GNT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17968. A4GNT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 340LumenalSequence analysisAdd BLAST315

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: ProtInc
  • membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi51146.
OpenTargetsiENSG00000118017.
PharmGKBiPA134960042.

Polymorphism and mutation databases

BioMutaiA4GNT.
DMDMi25452797.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805831 – 340Alpha-1,4-N-acetylglucosaminyltransferaseAdd BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9UNA3.
PRIDEiQ9UNA3.

PTM databases

PhosphoSitePlusiQ9UNA3.

Expressioni

Tissue specificityi

Detected in stomach and pancreas.

Gene expression databases

BgeeiENSG00000118017.
CleanExiHS_A4GNT.
GenevisibleiQ9UNA3. HS.

Organism-specific databases

HPAiHPA008017.

Interactioni

Protein-protein interaction databases

BioGridi119330. 20 interactors.
STRINGi9606.ENSP00000236709.

Structurei

3D structure databases

ProteinModelPortaliQ9UNA3.
SMRiQ9UNA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 169DXD motifBy similarity3

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
InParanoidiQ9UNA3.
KOiK08251.
OMAiFLNPQRF.
OrthoDBiEOG091G08S2.
PhylomeDBiQ9UNA3.
TreeFamiTF324053.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UNA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR
60 70 80 90 100
GIVFLETSER MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS
110 120 130 140 150
TYPAFSFLSA IDNVFLFPLD MKRLLEDTPL FSWYNQINAS AERNWLHISS
160 170 180 190 200
DASRLAIIWK YGGIYMDTDV ISIRPIPEEN FLAAQASRYS SNGIFGFLPH
210 220 230 240 250
HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED FQEVSDLRCL
260 270 280 290 300
NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV
310 320 330 340
IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK
Length:340
Mass (Da):39,497
Last modified:May 1, 2000 - v1
Checksum:i00D667A8394300AB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022096218A → D.1 PublicationCorresponds to variant rs2246945dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSiCCDS3097.1.
RefSeqiNP_057245.1. NM_016161.2.
XP_016862032.1. XM_017006543.1.
XP_016862033.1. XM_017006544.1.
UniGeneiHs.278960.

Genome annotation databases

EnsembliENST00000236709; ENSP00000236709; ENSG00000118017.
GeneIDi51146.
KEGGihsa:51146.
UCSCiuc003ers.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Alpha-1,4-N-acetylglucosaminyltransferase

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSiCCDS3097.1.
RefSeqiNP_057245.1. NM_016161.2.
XP_016862032.1. XM_017006543.1.
XP_016862033.1. XM_017006544.1.
UniGeneiHs.278960.

3D structure databases

ProteinModelPortaliQ9UNA3.
SMRiQ9UNA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119330. 20 interactors.
STRINGi9606.ENSP00000236709.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

PTM databases

PhosphoSitePlusiQ9UNA3.

Polymorphism and mutation databases

BioMutaiA4GNT.
DMDMi25452797.

Proteomic databases

PaxDbiQ9UNA3.
PRIDEiQ9UNA3.

Protocols and materials databases

DNASUi51146.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000236709; ENSP00000236709; ENSG00000118017.
GeneIDi51146.
KEGGihsa:51146.
UCSCiuc003ers.2. human.

Organism-specific databases

CTDi51146.
DisGeNETi51146.
GeneCardsiA4GNT.
HGNCiHGNC:17968. A4GNT.
HPAiHPA008017.
neXtProtiNX_Q9UNA3.
OpenTargetsiENSG00000118017.
PharmGKBiPA134960042.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
InParanoidiQ9UNA3.
KOiK08251.
OMAiFLNPQRF.
OrthoDBiEOG091G08S2.
PhylomeDBiQ9UNA3.
TreeFamiTF324053.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciZFISH:HS04186-MONOMER.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii51146.
PROiQ9UNA3.

Gene expression databases

BgeeiENSG00000118017.
CleanExiHS_A4GNT.
GenevisibleiQ9UNA3. HS.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA4GCT_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.