Reviewed, UniProtKB/Swiss-Prot Q9UNA3 (A4GCT_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha-1,4-N-acetylglucosaminyltransferase
      Short name=Alpha4GnT
    EC=2.4.1.-

Gene names

Name:

A4GNT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Detected in stomach and pancreas.
Domain	The conserved DXD motif is involved in enzyme activity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 32 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid O-linked glycosylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Ref.1 Traceable author statement. Source: ProtInc nucleolus Inferred from direct assay. Source: HPA
Molecular function	acetylglucosaminyltransferase activity Ref.1 Inferred from direct assay. Source: MGI galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Alpha-1,4-N-acetylglucosaminyltransferase		PRO_0000080583
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 25	21	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 340	315	Lumenal Potential
	Motif	167 – 169	3	DXD motif By similarity
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	218	1	A → D: dbSNP rs2246945. Ref.2		VAR_022096

Sequences

	Sequence		Length	Mass (Da)
	Q9UNA3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 00D667A8394300AB	FASTA	340	39,497
	10 20 30 40 50 60 MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER 70 80 90 100 110 120 MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD 130 140 150 160 170 180 MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN 190 200 210 220 230 240 FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED 250 260 270 280 290 300 FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV 310 320 330 340 IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin." Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1999) [PubMed: 10430883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Stomach.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-218.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Alpha-1,4-N-acetylglucosaminyltransferase

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF141315 mRNA. Translation: AAD48406.1. BC119639 mRNA. Translation: AAI19640.1. BC119640 mRNA. Translation: AAI19641.1.
IPI	IPI00007712.
RefSeq	NP_057245.1.
UniGene	Hs.278960
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT32. Glycosyltransferase Family 32.
Proteomic databases
PRIDE	Q9UNA3.
Genome annotation databases
Ensembl	ENSG00000118017. Homo sapiens. [Contig view]
GeneID	51146.
KEGG	hsa:51146.
Organism-specific databases
GeneCards	GC03M139325.
H-InvDB	HIX0030726.
HGNC	HGNC:17968. A4GNT.
HPA	HPA008017.
PharmGKB	PA134960042.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9UNA3.
HOVERGEN	Q9UNA3.
OMA	Q9UNA3. ESAAKIY.
Gene expression databases
ArrayExpress	Q9UNA3.
Bgee	Q9UNA3.
CleanEx	HS_A4GNT.
GermOnline	ENSG00000118017. Homo sapiens.
Family and domain databases
InterPro	IPR007652. Gb3_synth. IPR007577. Glyco_trans_s_bd. [Graphical view]
Pfam	PF04572. Gb3_synth. 1 hit. PF04488. Gly_transf_sug. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	54017.

Entry information

Entry name

A4GCT_HUMAN

Accession

Primary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)