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Protein

Alpha-1,4-N-acetylglucosaminyltransferase

Gene

A4GNT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.

Pathwayi

GO - Molecular functioni

  1. acetylglucosaminyltransferase activity Source: MGI

GO - Biological processi

  1. carbohydrate metabolic process Source: ProtInc
  2. glycoprotein biosynthetic process Source: MGI
  3. negative regulation of epithelial cell proliferation Source: Ensembl
  4. protein O-linked glycosylation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,4-N-acetylglucosaminyltransferase (EC:2.4.1.-)
Short name:
Alpha4GnT
Gene namesi
Name:A4GNT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17968. A4GNT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence Analysis
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 340315LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: ProtInc
  3. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134960042.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Alpha-1,4-N-acetylglucosaminyltransferasePRO_0000080583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9UNA3.
PRIDEiQ9UNA3.

Expressioni

Tissue specificityi

Detected in stomach and pancreas.

Gene expression databases

BgeeiQ9UNA3.
CleanExiHS_A4GNT.
GenevestigatoriQ9UNA3.

Organism-specific databases

HPAiHPA008017.

Interactioni

Protein-protein interaction databases

BioGridi119330. 19 interactions.
STRINGi9606.ENSP00000236709.

Structurei

3D structure databases

ProteinModelPortaliQ9UNA3.
SMRiQ9UNA3. Positions 124-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1693DXD motifBy similarity

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG251623.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
InParanoidiQ9UNA3.
KOiK08251.
OMAiCGFLYQF.
OrthoDBiEOG75MVW8.
PhylomeDBiQ9UNA3.
TreeFamiTF324053.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UNA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR
60 70 80 90 100
GIVFLETSER MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS
110 120 130 140 150
TYPAFSFLSA IDNVFLFPLD MKRLLEDTPL FSWYNQINAS AERNWLHISS
160 170 180 190 200
DASRLAIIWK YGGIYMDTDV ISIRPIPEEN FLAAQASRYS SNGIFGFLPH
210 220 230 240 250
HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED FQEVSDLRCL
260 270 280 290 300
NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV
310 320 330 340
IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK
Length:340
Mass (Da):39,497
Last modified:May 1, 2000 - v1
Checksum:i00D667A8394300AB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti218 – 2181A → D.1 Publication
Corresponds to variant rs2246945 [ dbSNP | Ensembl ].
VAR_022096

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSiCCDS3097.1.
RefSeqiNP_057245.1. NM_016161.2.
UniGeneiHs.278960.

Genome annotation databases

EnsembliENST00000236709; ENSP00000236709; ENSG00000118017.
GeneIDi51146.
KEGGihsa:51146.
UCSCiuc003ers.2. human.

Polymorphism databases

DMDMi25452797.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Alpha-1,4-N-acetylglucosaminyltransferase

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSiCCDS3097.1.
RefSeqiNP_057245.1. NM_016161.2.
UniGeneiHs.278960.

3D structure databases

ProteinModelPortaliQ9UNA3.
SMRiQ9UNA3. Positions 124-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119330. 19 interactions.
STRINGi9606.ENSP00000236709.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Polymorphism databases

DMDMi25452797.

Proteomic databases

PaxDbiQ9UNA3.
PRIDEiQ9UNA3.

Protocols and materials databases

DNASUi51146.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000236709; ENSP00000236709; ENSG00000118017.
GeneIDi51146.
KEGGihsa:51146.
UCSCiuc003ers.2. human.

Organism-specific databases

CTDi51146.
GeneCardsiGC03M137842.
HGNCiHGNC:17968. A4GNT.
HPAiHPA008017.
neXtProtiNX_Q9UNA3.
PharmGKBiPA134960042.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG251623.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
InParanoidiQ9UNA3.
KOiK08251.
OMAiCGFLYQF.
OrthoDBiEOG75MVW8.
PhylomeDBiQ9UNA3.
TreeFamiTF324053.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

GenomeRNAii51146.
NextBioi54017.
PROiQ9UNA3.

Gene expression databases

BgeeiQ9UNA3.
CleanExiHS_A4GNT.
GenevestigatoriQ9UNA3.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin."
    Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M.
    Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Stomach.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-218.

Entry informationi

Entry nameiA4GCT_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.