Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9UNA3 (A4GCT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,4-N-acetylglucosaminyltransferase

Short name=Alpha4GnT
EC=2.4.1.-
Gene names
Name:A4GNT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Probable.

Tissue specificity

Detected in stomach and pancreas.

Domain

The conserved DXD motif is involved in enzyme activity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 32 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Alpha-1,4-N-acetylglucosaminyltransferase
PRO_0000080583

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain26 – 340315Lumenal Potential
Motif167 – 1693DXD motif By similarity

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential

Natural variations

Natural variant2181A → D. Ref.2
Corresponds to variant rs2246945 [ dbSNP | Ensembl ].
VAR_022096

Sequences

Sequence LengthMass (Da)Tools
Q9UNA3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 00D667A8394300AB

FASTA34039,497
        10         20         30         40         50         60 
MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER 

        70         80         90        100        110        120 
MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD 

       130        140        150        160        170        180 
MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN 

       190        200        210        220        230        240 
FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED 

       250        260        270        280        290        300 
FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV 

       310        320        330        340 
IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin."
Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M.
Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Stomach.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-218.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Alpha-1,4-N-acetylglucosaminyltransferase

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
CCDSCCDS3097.1.
RefSeqNP_057245.1. NM_016161.2.
UniGeneHs.278960.

3D structure databases

ProteinModelPortalQ9UNA3.
SMRQ9UNA3. Positions 124-171.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000236709.

Protein family/group databases

CAZyGT32. Glycosyltransferase Family 32.

Polymorphism databases

DMDM25452797.

Proteomic databases

PaxDbQ9UNA3.
PRIDEQ9UNA3.

Protocols and materials databases

DNASU51146.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000236709; ENSP00000236709; ENSG00000118017.
GeneID51146.
KEGGhsa:51146.
UCSCuc003ers.2. human.

Organism-specific databases

CTD51146.
GeneCardsGC03M137842.
HGNCHGNC:17968. A4GNT.
HPAHPA008017.
neXtProtNX_Q9UNA3.
PharmGKBPA134960042.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG251623.
HOGENOMHOG000254772.
HOVERGENHBG106704.
InParanoidQ9UNA3.
KOK08251.
OMACGFLYQF.
OrthoDBEOG75MVW8.
PhylomeDBQ9UNA3.
TreeFamTF324053.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ9UNA3.
CleanExHS_A4GNT.
GenevestigatorQ9UNA3.

Family and domain databases

InterProIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi51146.
NextBio54017.
PROQ9UNA3.

Entry information

Entry nameA4GCT_HUMAN
AccessionPrimary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM