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Protein

Ras GTPase-activating protein-binding protein 2

Gene

G3BP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable scaffold protein that may be involved in mRNA transport.Curated

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • receptor signaling complex scaffold activity Source: UniProtKB

GO - Biological processi

  • cytoplasmic sequestering of NF-kappaB Source: UniProtKB
  • mRNA transport Source: UniProtKB-KW
  • Ras protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein-binding protein 2
Short name:
G3BP-2
Alternative name(s):
GAP SH3 domain-binding protein 2
Gene namesi
Name:G3BP2
Synonyms:KIAA0660
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:30291. G3BP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162389134.

Polymorphism and mutation databases

BioMutaiG3BP2.
DMDMi116242482.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 482481Ras GTPase-activating protein-binding protein 2PRO_0000194800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei141 – 1411PhosphoserineCombined sources
Modified residuei149 – 1491PhosphoserineCombined sources
Modified residuei225 – 2251PhosphoserineBy similarity
Modified residuei227 – 2271PhosphothreonineCombined sources
Cross-linki281 – 281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei392 – 3921N6-succinyllysineBy similarity
Modified residuei466 – 4661PhosphoserineCombined sources
Isoform B (identifier: Q9UN86-2)
Modified residuei227 – 2271PhosphothreonineCombined sources
Modified residuei235 – 2351PhosphoserineCombined sources

Post-translational modificationi

Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UN86.
MaxQBiQ9UN86.
PaxDbiQ9UN86.
PeptideAtlasiQ9UN86.
PRIDEiQ9UN86.

PTM databases

iPTMnetiQ9UN86.
PhosphoSiteiQ9UN86.

Expressioni

Gene expression databases

BgeeiENSG00000138757.
CleanExiHS_G3BP2.
ExpressionAtlasiQ9UN86. baseline and differential.
GenevisibleiQ9UN86. HS.

Organism-specific databases

HPAiHPA018304.
HPA018425.

Interactioni

Subunit structurei

Binds to the N-terminal domain of I-kappa-B-alpha.

Binary interactionsi

WithEntry#Exp.IntActNotes
USP10Q146945EBI-1044298,EBI-2510389

GO - Molecular functioni

  • receptor signaling complex scaffold activity Source: UniProtKB

Protein-protein interaction databases

BioGridi115237. 64 interactions.
DIPiDIP-50299N.
IntActiQ9UN86. 31 interactions.
MINTiMINT-3082700.
STRINGi9606.ENSP00000352738.

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi8 – 2518Combined sources
Helixi27 – 337Combined sources
Beta strandi34 – 4310Combined sources
Helixi58 – 669Combined sources
Beta strandi74 – 8411Combined sources
Helixi86 – 883Combined sources
Beta strandi90 – 9910Combined sources
Beta strandi106 – 11611Combined sources
Beta strandi118 – 13316Combined sources
Helixi134 – 1374Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DRVX-ray2.75A1-139[»]
ProteinModelPortaliQ9UN86.
SMRiQ9UN86. Positions 1-138, 308-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 133123NTF2PROSITE-ProRule annotationAdd
BLAST
Domaini331 – 40979RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi134 – 22390Glu-richAdd
BLAST
Compositional biasi419 – 47961Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0116. Eukaryota.
ENOG410YV57. LUCA.
GeneTreeiENSGT00390000011365.
HOGENOMiHOG000220838.
HOVERGENiHBG007211.
InParanoidiQ9UN86.
OMAiMEQNESD.
OrthoDBiEOG091G0GAN.
PhylomeDBiQ9UN86.
TreeFamiTF325464.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9UN86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMEKPSPLL VGREFVRQYY TLLNKAPEYL HRFYGRNSSY VHGGVDASGK
60 70 80 90 100
PQEAVYGQND IHHKVLSLNF SECHTKIRHV DAHATLSDGV VVQVMGLLSN
110 120 130 140 150
SGQPERKFMQ TFVLAPEGSV PNKFYVHNDM FRYEDEVFGD SEPELDEESE
160 170 180 190 200
DEVEEEQEER QPSPEPVQEN ANSGYYEAHP VTNGIEEPLE ESSHEPEPEP
210 220 230 240 250
ESETKTEELK PQVEEKNLEE LEEKSTTPPP AEPVSLPQEP PKAFSWASVT
260 270 280 290 300
SKNLPPSGTV SSSGIPPHVK APVSQPRVEA KPEVQSQPPR VREQRPRERP
310 320 330 340 350
GFPPRGPRPG RGDMEQNDSD NRRIIRYPDS HQLFVGNLPH DIDENELKEF
360 370 380 390 400
FMSFGNVVEL RINTKGVGGK LPNFGFVVFD DSEPVQRILI AKPIMFRGEV
410 420 430 440 450
RLNVEEKKTR AARERETRGG GDDRRDIRRN DRGPGGPRGI VGGGMMRDRD
460 470 480
GRGPPPRGGM AQKLGSGRGT GQMEGRFTGQ RR
Length:482
Mass (Da):54,121
Last modified:October 17, 2006 - v2
Checksum:i74B8EA43C0560229
GO
Isoform B (identifier: Q9UN86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-275: Missing.

Show »
Length:449
Mass (Da):50,817
Checksum:i1B0F021C138992E0
GO

Sequence cautioni

The sequence BAA31635 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti267 – 2671P → S in AAD51932 (Ref. 1) Curated
Sequence conflicti359 – 3591E → V in AAC15705 (Ref. 3) Curated
Sequence conflicti460 – 4601M → I in AAC15705 (Ref. 3) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti434 – 4341P → L in a breast cancer sample; somatic mutation. 1 Publication
VAR_036128

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei243 – 27533Missing in isoform B. 4 PublicationsVSP_003605Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145284 mRNA. Translation: AAD51932.1.
AB014560 mRNA. Translation: BAA31635.2. Different initiation.
AF051311 mRNA. Translation: AAC15705.1.
AF053535 mRNA. Translation: AAC95292.1.
AK291786 mRNA. Translation: BAF84475.1.
CH471057 Genomic DNA. Translation: EAX05742.1.
BC011731 mRNA. Translation: AAH11731.1.
CCDSiCCDS3571.1. [Q9UN86-1]
CCDS3572.1. [Q9UN86-2]
RefSeqiNP_036429.2. NM_012297.4. [Q9UN86-1]
NP_987100.1. NM_203504.2. [Q9UN86-2]
NP_987101.1. NM_203505.2. [Q9UN86-1]
XP_005263439.1. XM_005263382.2. [Q9UN86-1]
XP_005263440.1. XM_005263383.3. [Q9UN86-1]
XP_011530743.1. XM_011532441.2. [Q9UN86-1]
UniGeneiHs.303676.
Hs.613996.

Genome annotation databases

EnsembliENST00000357854; ENSP00000350518; ENSG00000138757. [Q9UN86-2]
ENST00000359707; ENSP00000352738; ENSG00000138757. [Q9UN86-1]
ENST00000395719; ENSP00000379069; ENSG00000138757. [Q9UN86-1]
GeneIDi9908.
KEGGihsa:9908.
UCSCiuc003hir.4. human. [Q9UN86-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145284 mRNA. Translation: AAD51932.1.
AB014560 mRNA. Translation: BAA31635.2. Different initiation.
AF051311 mRNA. Translation: AAC15705.1.
AF053535 mRNA. Translation: AAC95292.1.
AK291786 mRNA. Translation: BAF84475.1.
CH471057 Genomic DNA. Translation: EAX05742.1.
BC011731 mRNA. Translation: AAH11731.1.
CCDSiCCDS3571.1. [Q9UN86-1]
CCDS3572.1. [Q9UN86-2]
RefSeqiNP_036429.2. NM_012297.4. [Q9UN86-1]
NP_987100.1. NM_203504.2. [Q9UN86-2]
NP_987101.1. NM_203505.2. [Q9UN86-1]
XP_005263439.1. XM_005263382.2. [Q9UN86-1]
XP_005263440.1. XM_005263383.3. [Q9UN86-1]
XP_011530743.1. XM_011532441.2. [Q9UN86-1]
UniGeneiHs.303676.
Hs.613996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DRVX-ray2.75A1-139[»]
ProteinModelPortaliQ9UN86.
SMRiQ9UN86. Positions 1-138, 308-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115237. 64 interactions.
DIPiDIP-50299N.
IntActiQ9UN86. 31 interactions.
MINTiMINT-3082700.
STRINGi9606.ENSP00000352738.

PTM databases

iPTMnetiQ9UN86.
PhosphoSiteiQ9UN86.

Polymorphism and mutation databases

BioMutaiG3BP2.
DMDMi116242482.

Proteomic databases

EPDiQ9UN86.
MaxQBiQ9UN86.
PaxDbiQ9UN86.
PeptideAtlasiQ9UN86.
PRIDEiQ9UN86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357854; ENSP00000350518; ENSG00000138757. [Q9UN86-2]
ENST00000359707; ENSP00000352738; ENSG00000138757. [Q9UN86-1]
ENST00000395719; ENSP00000379069; ENSG00000138757. [Q9UN86-1]
GeneIDi9908.
KEGGihsa:9908.
UCSCiuc003hir.4. human. [Q9UN86-1]

Organism-specific databases

CTDi9908.
GeneCardsiG3BP2.
HGNCiHGNC:30291. G3BP2.
HPAiHPA018304.
HPA018425.
neXtProtiNX_Q9UN86.
PharmGKBiPA162389134.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0116. Eukaryota.
ENOG410YV57. LUCA.
GeneTreeiENSGT00390000011365.
HOGENOMiHOG000220838.
HOVERGENiHBG007211.
InParanoidiQ9UN86.
OMAiMEQNESD.
OrthoDBiEOG091G0GAN.
PhylomeDBiQ9UN86.
TreeFamiTF325464.

Miscellaneous databases

GeneWikiiG3BP2.
GenomeRNAii9908.
PROiQ9UN86.

Gene expression databases

BgeeiENSG00000138757.
CleanExiHS_G3BP2.
ExpressionAtlasiQ9UN86. baseline and differential.
GenevisibleiQ9UN86. HS.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3BP2_HUMAN
AccessioniPrimary (citable) accession number: Q9UN86
Secondary accession number(s): A8K6X1
, O60606, O75149, Q9UPA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: October 17, 2006
Last modified: September 7, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.