Q9UN86 (G3BP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating protein-binding protein 2
Short name=G3BP-2

Alternative name(s):
GAP SH3 domain-binding protein 2

Gene names

Name:	G3BP2
Synonyms:	KIAA0660

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable scaffold protein that may be involved in mRNA transport Potential.
Subunit structure	Binds to the N-terminal domain of I-kappa-B-alpha. Ref.9
Subcellular location	Cytoplasm.
Post-translational modification	Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine. Ref.10
Sequence similarities	Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain.
Sequence caution	The sequence BAA31635.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	RNA-binding
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Ras protein signal transduction Non-traceable author statement. Source: UniProtKB cytoplasmic sequestering of NF-kappaB Non-traceable author statement Ref.9. Source: UniProtKB mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	RNA binding Non-traceable author statement. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro receptor signaling complex scaffold activity Non-traceable author statement Ref.9. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9UN86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9UN86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     243-275: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 482	481	Ras GTPase-activating protein-binding protein 2		PRO_0000194800
Regions
	Domain	11 – 133	123	NTF2
	Domain	331 – 409	79	RRM
	Compositional bias	134 – 223	90	Glu-rich
	Compositional bias	419 – 479	61	Gly-rich
Sites
	Site	2	1	Not acetylated
Amino acid modifications
	Modified residue	141	1	Phosphoserine Ref.11 Ref.12 Ref.13 Ref.15
	Modified residue	149	1	Phosphoserine Ref.11 Ref.12 Ref.13 Ref.15
	Modified residue	225	1	Phosphoserine Ref.14
	Modified residue	226	1	Phosphothreonine Ref.14
	Modified residue	227	1	Phosphothreonine Ref.11 Ref.13 Ref.14 Ref.15
	Modified residue	235	1	Phosphoserine Ref.13
	Modified residue	457	1	Omega-N-methylated arginine Ref.10
	Modified residue	468	1	Omega-N-methylated arginine Ref.10
Natural variations
	Alternative sequence	243 – 275	33	Missing in isoform B.		VSP_003605
	Natural variant	434	1	P → L in a breast cancer sample; somatic mutation. Ref.17		VAR_036128
Experimental info
	Sequence conflict	267	1	P → S in AAD51932. Ref.1
	Sequence conflict	359	1	E → V in AAC15705. Ref.3
	Sequence conflict	460	1	M → I in AAC15705. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 74B8EA43C0560229	FASTA	482	54,121
	10 20 30 40 50 60 MVMEKPSPLL VGREFVRQYY TLLNKAPEYL HRFYGRNSSY VHGGVDASGK PQEAVYGQND 70 80 90 100 110 120 IHHKVLSLNF SECHTKIRHV DAHATLSDGV VVQVMGLLSN SGQPERKFMQ TFVLAPEGSV 130 140 150 160 170 180 PNKFYVHNDM FRYEDEVFGD SEPELDEESE DEVEEEQEER QPSPEPVQEN ANSGYYEAHP 190 200 210 220 230 240 VTNGIEEPLE ESSHEPEPEP ESETKTEELK PQVEEKNLEE LEEKSTTPPP AEPVSLPQEP 250 260 270 280 290 300 PKAFSWASVT SKNLPPSGTV SSSGIPPHVK APVSQPRVEA KPEVQSQPPR VREQRPRERP 310 320 330 340 350 360 GFPPRGPRPG RGDMEQNDSD NRRIIRYPDS HQLFVGNLPH DIDENELKEF FMSFGNVVEL 370 380 390 400 410 420 RINTKGVGGK LPNFGFVVFD DSEPVQRILI AKPIMFRGEV RLNVEEKKTR AARERETRGG 430 440 450 460 470 480 GDDRRDIRRN DRGPGGPRGI VGGGMMRDRD GRGPPPRGGM AQKLGSGRGT GQMEGRFTGQ RR
	Isoform B [UniParc]. Checksum: 1B0F021C138992E0	FASTA	449	50,817
	10 20 30 40 50 60 MVMEKPSPLL VGREFVRQYY TLLNKAPEYL HRFYGRNSSY VHGGVDASGK PQEAVYGQND 70 80 90 100 110 120 IHHKVLSLNF SECHTKIRHV DAHATLSDGV VVQVMGLLSN SGQPERKFMQ TFVLAPEGSV 130 140 150 160 170 180 PNKFYVHNDM FRYEDEVFGD SEPELDEESE DEVEEEQEER QPSPEPVQEN ANSGYYEAHP 190 200 210 220 230 240 VTNGIEEPLE ESSHEPEPEP ESETKTEELK PQVEEKNLEE LEEKSTTPPP AEPVSLPQEP 250 260 270 280 290 300 PKPRVEAKPE VQSQPPRVRE QRPRERPGFP PRGPRPGRGD MEQNDSDNRR IIRYPDSHQL 310 320 330 340 350 360 FVGNLPHDID ENELKEFFMS FGNVVELRIN TKGVGGKLPN FGFVVFDDSE PVQRILIAKP 370 380 390 400 410 420 IMFRGEVRLN VEEKKTRAAR ERETRGGGDD RRDIRRNDRG PGGPRGIVGG GMMRDRDGRG 430 440 PPPRGGMAQK LGSGRGTGQM EGRFTGQRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation and chromosomal location of G3BP-1 and G3BP-2a/b, members of a novel SH3 domain-binding and RNA-binding protein family implicated in signal transduction." Kennedy D., Mattick J.S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B). Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Brain.
[3]	Guitard E. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Brain.
[4]	Kennedy D., Ru K., Mattick J.S. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Placenta.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Tissue: B-cell.
[8]	Bienvenut W.V., Heiserich L., Boulahbel H., Gottlieb E., Vousden K.H., Lukashchuk N., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 18-32; 124-132; 206-224; 243-252; 278-290; 371-397 AND 439-447, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY. Tissue: Colon carcinoma, Lung carcinoma and Ovarian carcinoma.
[9]	"IkappaBalpha and IkappaBalpha /NF-kappa B complexes are retained in the cytoplasm through interaction with a novel partner, RasGAP SH3-binding protein 2." Prigent M., Barlat I., Langen H., Dargemont C. J. Biol. Chem. 275:36441-36449(2000) [PubMed: 10969074] [Abstract] Cited for: INTERACTION WITH IKAPPABALPHA.
[10]	"Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." Ong S.E., Mittler G., Mann M. Nat. Methods 1:119-126(2004) [PubMed: 15782174] [Abstract] Cited for: METHYLATION [LARGE SCALE ANALYSIS] AT ARG-457 AND ARG-468, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-149 AND THR-227, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141 AND SER-149, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-149; SER-235 AND THR-227, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; THR-226 AND THR-227, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-149 AND THR-227, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-434.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF145284 mRNA. Translation: AAD51932.1. AB014560 mRNA. Translation: BAA31635.2. Different initiation. AF051311 mRNA. Translation: AAC15705.1. AF053535 mRNA. Translation: AAC95292.1. AK291786 mRNA. Translation: BAF84475.1. CH471057 Genomic DNA. Translation: EAX05742.1. BC011731 mRNA. Translation: AAH11731.1.
IPI	IPI00009057. IPI00179890.
RefSeq	NP_036429.2. NM_012297.4. NP_987100.1. NM_203504.2. NP_987101.1. NM_203505.2.
UniGene	Hs.303676.
3D structure databases
ProteinModelPortal	Q9UN86.
SMR	Q9UN86. Positions 1-139, 328-407.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UN86. 25 interactions.
MINT	MINT-3082700.
STRING	Q9UN86.
PTM databases
PhosphoSite	Q9UN86.
Polymorphism databases
DMDM	116242482.
Proteomic databases
PRIDE	Q9UN86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359707; ENSP00000352738; ENSG00000138757. ENST00000395719; ENSP00000379069; ENSG00000138757.
GeneID	9908.
KEGG	hsa:9908.
UCSC	uc003hir.1. human. uc003hit.1. human.
Organism-specific databases
CTD	9908.
GeneCards	GC04M076567.
H-InvDB	HIX0004298.
HGNC	HGNC:30291. G3BP2.
HPA	HPA018304. HPA018425.
neXtProt	NX_Q9UN86.
PharmGKB	PA162389134.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13856.
GeneTree	ENSGT00390000011365.
HOGENOM	HBG445300.
HOVERGEN	HBG007211.
InParanoid	Q9UN86.
OMA	AGQMEGR.
OrthoDB	EOG4XWFZ0.
PhylomeDB	Q9UN86.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9UN86.
Bgee	Q9UN86.
CleanEx	HS_G3BP2.
Genevestigator	Q9UN86.
GermOnline	ENSG00000138757. Homo sapiens.
Family and domain databases
InterPro	IPR002075. NTF2. IPR018222. Nuclear_transport_factor_2_euk. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF02136. NTF2. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50177. NTF2_DOMAIN. 1 hit. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	37365.

Entry information

Entry name

G3BP2_HUMAN

Accession

Primary (citable) accession number: Q9UN86
Secondary accession number(s): A8K6X1 , O60606, O75149, Q9UPA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.