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Protein

LINE-1 retrotransposable element ORF1 protein

Gene

L1RE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.3 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: UniProtKB
  • single-stranded RNA binding Source: UniProtKB

GO - Biological processi

  • transposition, RNA-mediated Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LINE-1 retrotransposable element ORF1 protein
Short name:
L1ORF1p
Alternative name(s):
LINE retrotransposable element 1
LINE1 retrotransposable element 1
Gene namesi
Name:L1RE1
Synonyms:LRE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:6686. L1RE1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleusnucleolus 1 Publication

  • Note: Colocalizes with cytoplasmic ribonucleoprotein particle (RNP) (PubMed:16183655). Identified with its own transcript in a cytoplasmic ribonucleoprotein complex (PubMed:16183655). Mainly cytoplasmic where it is enriched in stress granules but also more rarely detected in the nucleolus (PubMed:17562864).2 Publications

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic stress granule Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi116 – 1161E → A: Loss of LINE-1 retrotransposition without affecting RNA-binding; when associated with A-123. 1 Publication
Mutagenesisi123 – 1231D → A: Loss of LINE-1 retrotransposition without affecting RNA-binding; when associated with A-116. 1 Publication
Mutagenesisi132 – 1321G → I: Loss of LINE-1 retrotransposition without affecting RNA-binding; when associated with I-135 and I-142. 1 Publication
Mutagenesisi133 – 1331K → S: Loss of LINE-1 retrotransposition with decreased RNA-binding; when associated with A-137, A-140 and S-141. 1 Publication
Mutagenesisi135 – 1351R → I: Loss of LINE-1 retrotransposition without effect on RNA-binding; when associated with I-132 and I-142. 1 Publication
Mutagenesisi137 – 1371K → A: Loss of LINE-1 retrotransposition with decreased RNA-binding; when associated with S-133, A-140 and S-141. 1 Publication
Mutagenesisi140 – 1401K → A: Loss of LINE-1 retrotransposition with decreased RNA-binding; when associated with S-133, A-137 and S-141. 1 Publication
Mutagenesisi141 – 1411R → S: Loss of LINE-1 retrotransposition with decreased RNA-binding; when associated with S-133, A-137 and A-140. 1 Publication
Mutagenesisi142 – 1421N → I: Loss of LINE-1 retrotransposition without effect on RNA-binding; when associated with I-132 and I-135. 1 Publication
Mutagenesisi159 – 1591R → A: Moderately alters RNA-binding activity. 1 Publication
Mutagenesisi206 – 2061R → A: Loss of RNA-binding activity; when associated with A-210 and A-211. 2 Publications
Mutagenesisi210 – 2101R → A: Loss of RNA-binding activity; when associated with A-206 and A-211. 2 Publications
Mutagenesisi211 – 2111R → A: Loss of RNA-binding activity; when associated with A-206 and A-210. 2 Publications
Mutagenesisi218 – 2181I → Y: Moderately alters RNA-binding activity. 2 Publications
Mutagenesisi220 – 2201R → A: Loss of LINE-1 retrotransposition without affecting RNA-binding. 2 Publications
Mutagenesisi235 – 2351R → A: Loss of LINE-1 retrotransposition with decreased RNA-binding. 2 Publications
Mutagenesisi261 – 2611R → A: Loss of LINE-1 retrotransposition with decreased RNA-binding. 2 Publications
Mutagenesisi261 – 2611R → K: Loss of LINE-1 retrotransposition without affecting RNA-binding. 2 Publications
Mutagenesisi282 – 2821Y → A: Loss of LINE-1 retrotransposition without affecting RNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338LINE-1 retrotransposable element ORF1 proteinPRO_0000425081Add
BLAST

Proteomic databases

EPDiQ9UN81.
MaxQBiQ9UN81.
PeptideAtlasiQ9UN81.

PTM databases

iPTMnetiQ9UN81.
PhosphoSiteiQ9UN81.

Interactioni

Subunit structurei

Homotrimer (via coiled coil domain). May also form larger homooligomers. May interact with DDX39A, HNRNPA1, SERBP1 and YBX1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q8TE3015EBI-722458,EBI-8874509
UPF1Q929006EBI-722458,EBI-373471

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-48676N.
IntActiQ9UN81. 20 interactions.

Structurei

Secondary structure

1
338
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi111 – 15343Combined sources
Turni154 – 1563Combined sources
Beta strandi157 – 1615Combined sources
Helixi171 – 18515Combined sources
Helixi187 – 1926Combined sources
Beta strandi197 – 20610Combined sources
Beta strandi210 – 2134Combined sources
Beta strandi216 – 2227Combined sources
Helixi224 – 23714Combined sources
Beta strandi240 – 2423Combined sources
Beta strandi245 – 2506Combined sources
Helixi255 – 2639Combined sources
Helixi265 – 2739Combined sources
Beta strandi279 – 2813Combined sources
Turni282 – 2843Combined sources
Beta strandi285 – 29915Combined sources
Helixi300 – 3078Combined sources
Helixi311 – 3177Combined sources
Turni318 – 3225Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LDYNMR-A157-330[»]
2W7AX-ray1.40A/B157-250[»]
2YKOX-ray2.10A/B/C106-330[»]
2YKPX-ray3.10A/B/C106-326[»]
2YKQX-ray3.10A/B/C106-326[»]
ProteinModelPortaliQ9UN81.
SMRiQ9UN81. Positions 110-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 25296RNA recognition motif (RRM) domainAdd
BLAST
Regioni253 – 31765C-terminal domain (CTD)Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili49 – 153105Sequence analysisAdd
BLAST

Domaini

The coiled coil domain mediates homotrimerization.
The RRM and the CTD domain are both required for proper RNA-binding activity.

Sequence similaritiesi

Belongs to the transposase 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG008774.

Family and domain databases

InterProiIPR004244. Transposase_22.
[Graphical view]
PANTHERiPTHR11505. PTHR11505. 1 hit.
PfamiPF02994. Transposase_22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UN81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKQNRKTG NSKTQSASPP PKERSSSPAT EQSWMENDFD ELREEGFRRS
60 70 80 90 100
NYSELREDIQ TKGKEVENFE KNLEECITRI TNTEKCLKEL MELKTKAREL
110 120 130 140 150
REECRSLRSR CDQLEERVSA MEDEMNEMKR EGKFREKRIK RNEQSLQEIW
160 170 180 190 200
DYVKRPNLRL IGVPESDVEN GTKLENTLQD IIQENFPNLA RQANVQIQEI
210 220 230 240 250
QRTPQRYSSR RATPRHIIVR FTKVEMKEKM LRAAREKGRV TLKGKPIRLT
260 270 280 290 300
ADLSAETLQA RREWGPIFNI LKEKNFQPRI SYPAKLSFIS EGEIKYFIDK
310 320 330
QMLRDFVTTR PALKELLKEA LNMERNNRYQ PLQNHAKM
Length:338
Mass (Da):40,056
Last modified:May 1, 2000 - v1
Checksum:iC9ED80C33ED37523
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti251 – 2511A → V in AAB59367 (PubMed:10655552).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148856 Genomic DNA. Translation: AAD39214.1.
M80343 Genomic DNA. Translation: AAB59367.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148856 Genomic DNA. Translation: AAD39214.1.
M80343 Genomic DNA. Translation: AAB59367.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LDYNMR-A157-330[»]
2W7AX-ray1.40A/B157-250[»]
2YKOX-ray2.10A/B/C106-330[»]
2YKPX-ray3.10A/B/C106-326[»]
2YKQX-ray3.10A/B/C106-326[»]
ProteinModelPortaliQ9UN81.
SMRiQ9UN81. Positions 110-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48676N.
IntActiQ9UN81. 20 interactions.

PTM databases

iPTMnetiQ9UN81.
PhosphoSiteiQ9UN81.

Proteomic databases

EPDiQ9UN81.
MaxQBiQ9UN81.
PeptideAtlasiQ9UN81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GeneCardsiL1RE1.
HGNCiHGNC:6686. L1RE1.
MIMi151626. gene.
neXtProtiNX_Q9UN81.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG008774.

Miscellaneous databases

PROiQ9UN81.
SOURCEiSearch...

Family and domain databases

InterProiIPR004244. Transposase_22.
[Graphical view]
PANTHERiPTHR11505. PTHR11505. 1 hit.
PfamiPF02994. Transposase_22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLORF1_HUMAN
AccessioniPrimary (citable) accession number: Q9UN81
Secondary accession number(s): Q15605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but one estimate that 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing two non-overlapping ORFs, ORF1 and ORF2. ORF1p is described in that entry as a representative of all ORF1p potentially expressed by active elements. ORF2p is described in the related entry AC O00370.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.