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Q9UN19 (DAPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

Short name=hDAPP1
Alternative name(s):
B lymphocyte adapter protein Bam32
B-cell adapter molecule of 32 kDa
Gene names
Name:DAPP1
Synonyms:BAM32
ORF Names:HSPC066
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K. Ref.2

Subunit structure

Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2. Ref.1 Ref.2

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein. Note: Membrane-associated after cell stimulation leading to its translocation. Ref.2

Tissue specificity

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells. Ref.1

Induction

Upon B-cell activation.

Post-translational modification

Phosphorylated on tyrosine residues. Ref.2

Sequence similarities

Contains 1 PH domain.

Contains 1 SH2 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UN19-1)

Also known as: hBam1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UN19-2)

Also known as: hBam2;

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: SQIRKQLNQGEGTIRSRSFIFK → VKDKSCILSALCISPEEKTDHK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 280280Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
PRO_0000079785

Regions

Domain35 – 12995SH2
Domain164 – 25996PH

Amino acid modifications

Modified residue1391Phosphotyrosine By similarity
Modified residue1411Phosphoserine By similarity

Natural variations

Alternative sequence259 – 28022SQIRK…SFIFK → VKDKSCILSALCISPEEKTD HK in isoform 2.
VSP_010699

Experimental info

Mutagenesis611R → K: No change in BCR-induced NFAT activation. Ref.2
Mutagenesis1731K → L: No interaction with 3-phosphoinositides. Ref.1
Mutagenesis1841R → C: No membrane association. Ref.2
Mutagenesis1971K → E: No membrane association. Ref.2
Mutagenesis2501W → L: No interaction with 3-phosphoinositides. Ref.1
Sequence conflict216 – 22914VQFDY…VNCFC → MICNILCSFFCPIS Ref.6

Secondary structure

.................... 280
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (hBam1) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 4A7F686B55146241

FASTA28032,194
        10         20         30         40         50         60 
MGRAELLEGK MSTQDPSDLW SRSDGEAELL QDLGWYHGNL TRHAAEALLL SNGCDGSYLL 

        70         80         90        100        110        120 
RDSNETTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEFS SLKDFVKHFA NQPLIGSETG 

       130        140        150        160        170        180 
TLMVLKHPYP RKVEEPSIYE SVRVHTAMQT GRTEDDLVPT APSLGTKEGY LTKQGGLVKT 

       190        200        210        220        230        240 
WKTRWFTLHR NELKYFKDQM SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC 

       250        260        270        280 
AKTGVEADEW IKILRWKLSQ IRKQLNQGEG TIRSRSFIFK 

« Hide

Isoform 2 (hBam2) [UniParc].

Checksum: 643DF0A3C4FB32DC
Show »

FASTA28032,044

References

« Hide 'large scale' references
[1]"DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides."
Dowler S., Currie R.A., Downes C.P., Alessi D.R.
Biochem. J. 342:7-12(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH PTDINS(3,4,5)P3 AND PTDINS(3,4)P2, MUTAGENESIS OF LYS-173 AND TRP-250.
[2]"A novel B lymphocyte-associated adaptor protein, Bam32, regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase."
Marshall A.J., Niiro H., Lerner C.G., Yun T.J., Thomas S., Disteche C.M., Clark E.A.
J. Exp. Med. 191:1319-1332(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, INTERACTION WITH PLCG2, MUTAGENESIS OF ARG-61; ARG-184 AND LYS-197.
[3]"Molecular cloning of a protein-tyrosine phosphatase D."
Zhang W., Wan T., Cao X.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Umbilical cord blood.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Urinary bladder wart.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 216-280 (ISOFORM 1).
Tissue: Lymph node.
[7]"Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains."
Ferguson K.M., Kavran J.M., Sankaran V.G., Fournier E., Isakoff S.J., Skolnik E.Y., Lemmon M.A.
Mol. Cell 6:373-384(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 162-261 IN COMPLEX WITH INOSITOL 1,3,4,5-3 TETRAKISPHOSPHATE, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 157-262.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF163254 mRNA. Translation: AAD49697.1.
AF161551 mRNA. Translation: AAF29038.1.
AF186022 mRNA. Translation: AAF14578.1.
AF178987 mRNA. Translation: AAF44351.1.
BC012924 mRNA. Translation: AAH12924.1.
AL713793 mRNA. Translation: CAD28547.1.
CCDSCCDS47112.1. [Q9UN19-1]
RefSeqNP_055210.2. NM_014395.2. [Q9UN19-1]
UniGeneHs.436271.
Hs.708484.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FAOX-ray1.80A148-273[»]
1FB8X-ray2.40A148-273[»]
ProteinModelPortalQ9UN19.
SMRQ9UN19. Positions 35-128, 157-262.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117981. 5 interactions.
IntActQ9UN19. 6 interactions.
MINTMINT-1499560.
STRING9606.ENSP00000296414.

PTM databases

PhosphoSiteQ9UN19.

Polymorphism databases

DMDM51317293.

Proteomic databases

MaxQBQ9UN19.
PaxDbQ9UN19.
PRIDEQ9UN19.

Protocols and materials databases

DNASU27071.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000512369; ENSP00000423602; ENSG00000070190. [Q9UN19-1]
GeneID27071.
KEGGhsa:27071.
UCSCuc003hvf.4. human. [Q9UN19-1]

Organism-specific databases

CTD27071.
GeneCardsGC04P100737.
H-InvDBHIX0004401.
HGNCHGNC:16500. DAPP1.
HPACAB033822.
HPA046074.
MIM605768. gene.
neXtProtNX_Q9UN19.
PharmGKBPA27145.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145370.
HOGENOMHOG000112147.
HOVERGENHBG051297.
InParanoidQ9UN19.
KOK12229.
OMAQRNELKY.
PhylomeDBQ9UN19.
TreeFamTF105418.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkQ9UN19.

Gene expression databases

ArrayExpressQ9UN19.
BgeeQ9UN19.
CleanExHS_DAPP1.
GenevestigatorQ9UN19.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000980. SH2.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
SMARTSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9UN19.
GeneWikiDAPP1.
GenomeRNAi27071.
NextBio49659.
PROQ9UN19.
SOURCESearch...

Entry information

Entry nameDAPP1_HUMAN
AccessionPrimary (citable) accession number: Q9UN19
Secondary accession number(s): Q8TCK5, Q9UHF2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM