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Q9UN19

- DAPP1_HUMAN

UniProt

Q9UN19 - DAPP1_HUMAN

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Protein
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Gene
DAPP1, BAM32, HSPC066
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.1 Publication

GO - Molecular functioni

  1. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  2. phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  3. phospholipid binding Source: UniProtKB

GO - Biological processi

  1. protein dephosphorylation Source: UniProtKB
  2. signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_118700. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiQ9UN19.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Short name:
hDAPP1
Alternative name(s):
B lymphocyte adapter protein Bam32
B-cell adapter molecule of 32 kDa
Gene namesi
Name:DAPP1
Synonyms:BAM32
ORF Names:HSPC066
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:16500. DAPP1.

Subcellular locationi

Cytoplasm. Membrane; Peripheral membrane protein
Note: Membrane-associated after cell stimulation leading to its translocation.1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi61 – 611R → K: No change in BCR-induced NFAT activation. 1 Publication
Mutagenesisi173 – 1731K → L: No interaction with 3-phosphoinositides. 1 Publication
Mutagenesisi184 – 1841R → C: No membrane association. 1 Publication
Mutagenesisi197 – 1971K → E: No membrane association. 1 Publication
Mutagenesisi250 – 2501W → L: No interaction with 3-phosphoinositides. 1 Publication

Organism-specific databases

PharmGKBiPA27145.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
PRO_0000079785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391Phosphotyrosine By similarity
Modified residuei141 – 1411Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UN19.
PaxDbiQ9UN19.
PRIDEiQ9UN19.

PTM databases

PhosphoSiteiQ9UN19.

Expressioni

Tissue specificityi

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.1 Publication

Inductioni

Upon B-cell activation.

Gene expression databases

ArrayExpressiQ9UN19.
BgeeiQ9UN19.
CleanExiHS_DAPP1.
GenevestigatoriQ9UN19.

Organism-specific databases

HPAiCAB033822.
HPA046074.

Interactioni

Subunit structurei

Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.2 Publications

Protein-protein interaction databases

BioGridi117981. 5 interactions.
IntActiQ9UN19. 6 interactions.
MINTiMINT-1499560.
STRINGi9606.ENSP00000296414.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi167 – 1748
Beta strandi176 – 1783
Beta strandi181 – 1899
Beta strandi192 – 1987
Beta strandi205 – 2095
Helixi210 – 2123
Beta strandi215 – 2195
Beta strandi221 – 23212
Beta strandi235 – 2406
Helixi244 – 25916

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FAOX-ray1.80A148-273[»]
1FB8X-ray2.40A148-273[»]
ProteinModelPortaliQ9UN19.
SMRiQ9UN19. Positions 35-128, 157-262.

Miscellaneous databases

EvolutionaryTraceiQ9UN19.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 12995SH2
Add
BLAST
Domaini164 – 25996PH
Add
BLAST

Sequence similaritiesi

Contains 1 PH domain.
Contains 1 SH2 domain.

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG145370.
HOGENOMiHOG000112147.
HOVERGENiHBG051297.
InParanoidiQ9UN19.
KOiK12229.
OMAiQRNELKY.
PhylomeDBiQ9UN19.
TreeFamiTF105418.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UN19-1) [UniParc]FASTAAdd to Basket

Also known as: hBam1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGRAELLEGK MSTQDPSDLW SRSDGEAELL QDLGWYHGNL TRHAAEALLL    50
SNGCDGSYLL RDSNETTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEFS 100
SLKDFVKHFA NQPLIGSETG TLMVLKHPYP RKVEEPSIYE SVRVHTAMQT 150
GRTEDDLVPT APSLGTKEGY LTKQGGLVKT WKTRWFTLHR NELKYFKDQM 200
SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC AKTGVEADEW 250
IKILRWKLSQ IRKQLNQGEG TIRSRSFIFK 280
Length:280
Mass (Da):32,194
Last modified:May 1, 2000 - v1
Checksum:i4A7F686B55146241
GO
Isoform 2 (identifier: Q9UN19-2) [UniParc]FASTAAdd to Basket

Also known as: hBam2

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: SQIRKQLNQGEGTIRSRSFIFK → VKDKSCILSALCISPEEKTDHK

Show »
Length:280
Mass (Da):32,044
Checksum:i643DF0A3C4FB32DC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei259 – 28022SQIRK…SFIFK → VKDKSCILSALCISPEEKTD HK in isoform 2.
VSP_010699Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 22914VQFDY…VNCFC → MICNILCSFFCPIS1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163254 mRNA. Translation: AAD49697.1.
AF161551 mRNA. Translation: AAF29038.1.
AF186022 mRNA. Translation: AAF14578.1.
AF178987 mRNA. Translation: AAF44351.1.
BC012924 mRNA. Translation: AAH12924.1.
AL713793 mRNA. Translation: CAD28547.1.
CCDSiCCDS47112.1. [Q9UN19-1]
RefSeqiNP_055210.2. NM_014395.2. [Q9UN19-1]
UniGeneiHs.436271.
Hs.708484.

Genome annotation databases

EnsembliENST00000512369; ENSP00000423602; ENSG00000070190. [Q9UN19-1]
GeneIDi27071.
KEGGihsa:27071.
UCSCiuc003hvf.4. human. [Q9UN19-1]

Polymorphism databases

DMDMi51317293.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163254 mRNA. Translation: AAD49697.1 .
AF161551 mRNA. Translation: AAF29038.1 .
AF186022 mRNA. Translation: AAF14578.1 .
AF178987 mRNA. Translation: AAF44351.1 .
BC012924 mRNA. Translation: AAH12924.1 .
AL713793 mRNA. Translation: CAD28547.1 .
CCDSi CCDS47112.1. [Q9UN19-1 ]
RefSeqi NP_055210.2. NM_014395.2. [Q9UN19-1 ]
UniGenei Hs.436271.
Hs.708484.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FAO X-ray 1.80 A 148-273 [» ]
1FB8 X-ray 2.40 A 148-273 [» ]
ProteinModelPortali Q9UN19.
SMRi Q9UN19. Positions 35-128, 157-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117981. 5 interactions.
IntActi Q9UN19. 6 interactions.
MINTi MINT-1499560.
STRINGi 9606.ENSP00000296414.

PTM databases

PhosphoSitei Q9UN19.

Polymorphism databases

DMDMi 51317293.

Proteomic databases

MaxQBi Q9UN19.
PaxDbi Q9UN19.
PRIDEi Q9UN19.

Protocols and materials databases

DNASUi 27071.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000512369 ; ENSP00000423602 ; ENSG00000070190 . [Q9UN19-1 ]
GeneIDi 27071.
KEGGi hsa:27071.
UCSCi uc003hvf.4. human. [Q9UN19-1 ]

Organism-specific databases

CTDi 27071.
GeneCardsi GC04P100737.
H-InvDB HIX0004401.
HGNCi HGNC:16500. DAPP1.
HPAi CAB033822.
HPA046074.
MIMi 605768. gene.
neXtProti NX_Q9UN19.
PharmGKBi PA27145.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG145370.
HOGENOMi HOG000112147.
HOVERGENi HBG051297.
InParanoidi Q9UN19.
KOi K12229.
OMAi QRNELKY.
PhylomeDBi Q9UN19.
TreeFami TF105418.

Enzyme and pathway databases

Reactomei REACT_118700. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinki Q9UN19.

Miscellaneous databases

EvolutionaryTracei Q9UN19.
GeneWikii DAPP1.
GenomeRNAii 27071.
NextBioi 49659.
PROi Q9UN19.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9UN19.
Bgeei Q9UN19.
CleanExi HS_DAPP1.
Genevestigatori Q9UN19.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
SMARTi SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view ]
SUPFAMi SSF55550. SSF55550. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides."
    Dowler S., Currie R.A., Downes C.P., Alessi D.R.
    Biochem. J. 342:7-12(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH PTDINS(3,4,5)P3 AND PTDINS(3,4)P2, MUTAGENESIS OF LYS-173 AND TRP-250.
  2. "A novel B lymphocyte-associated adaptor protein, Bam32, regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase."
    Marshall A.J., Niiro H., Lerner C.G., Yun T.J., Thomas S., Disteche C.M., Clark E.A.
    J. Exp. Med. 191:1319-1332(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, INTERACTION WITH PLCG2, MUTAGENESIS OF ARG-61; ARG-184 AND LYS-197.
  3. "Molecular cloning of a protein-tyrosine phosphatase D."
    Zhang W., Wan T., Cao X.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Urinary bladder wart.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 216-280 (ISOFORM 1).
    Tissue: Lymph node.
  7. "Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains."
    Ferguson K.M., Kavran J.M., Sankaran V.G., Fournier E., Isakoff S.J., Skolnik E.Y., Lemmon M.A.
    Mol. Cell 6:373-384(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 162-261 IN COMPLEX WITH INOSITOL 1,3,4,5-3 TETRAKISPHOSPHATE, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 157-262.

Entry informationi

Entry nameiDAPP1_HUMAN
AccessioniPrimary (citable) accession number: Q9UN19
Secondary accession number(s): Q8TCK5, Q9UHF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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