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Protein

Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

Gene

DAPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  • phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • protein dephosphorylation Source: UniProtKB
  • signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiQ9UN19.
SIGNORiQ9UN19.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Short name:
hDAPP1
Alternative name(s):
B lymphocyte adapter protein Bam32
B-cell adapter molecule of 32 kDa
Gene namesi
Name:DAPP1
Synonyms:BAM32
ORF Names:HSPC066
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:16500. DAPP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61R → K: No change in BCR-induced NFAT activation. 1 Publication1
Mutagenesisi173K → L: No interaction with 3-phosphoinositides. 1 Publication1
Mutagenesisi184R → C: No membrane association. 1 Publication1
Mutagenesisi197K → E: No membrane association. 1 Publication1
Mutagenesisi250W → L: No interaction with 3-phosphoinositides. 1 Publication1

Organism-specific databases

DisGeNETi27071.
OpenTargetsiENSG00000070190.
PharmGKBiPA27145.

Polymorphism and mutation databases

BioMutaiDAPP1.
DMDMi51317293.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797851 – 280Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositideAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphotyrosineBy similarity1
Modified residuei141PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9UN19.
PeptideAtlasiQ9UN19.
PRIDEiQ9UN19.

PTM databases

iPTMnetiQ9UN19.
PhosphoSitePlusiQ9UN19.

Expressioni

Tissue specificityi

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.1 Publication

Inductioni

Upon B-cell activation.

Gene expression databases

BgeeiENSG00000070190.
CleanExiHS_DAPP1.
ExpressionAtlasiQ9UN19. baseline and differential.
GenevisibleiQ9UN19. HS.

Organism-specific databases

HPAiCAB033822.
HPA046074.

Interactioni

Subunit structurei

Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APBB3O957042EBI-3918199,EBI-286427
WDR20Q8TBZ33EBI-3918199,EBI-2511486

Protein-protein interaction databases

BioGridi117981. 16 interactors.
IntActiQ9UN19. 18 interactors.
MINTiMINT-1499560.
STRINGi9606.ENSP00000423602.

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi167 – 174Combined sources8
Beta strandi176 – 178Combined sources3
Beta strandi181 – 189Combined sources9
Beta strandi192 – 198Combined sources7
Beta strandi205 – 209Combined sources5
Helixi210 – 212Combined sources3
Beta strandi215 – 219Combined sources5
Beta strandi221 – 232Combined sources12
Beta strandi235 – 240Combined sources6
Helixi244 – 259Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAOX-ray1.80A148-273[»]
1FB8X-ray2.40A148-273[»]
ProteinModelPortaliQ9UN19.
SMRiQ9UN19.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UN19.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 129SH2PROSITE-ProRule annotationAdd BLAST95
Domaini164 – 259PHPROSITE-ProRule annotationAdd BLAST96

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IF6C. Eukaryota.
ENOG410XSW5. LUCA.
GeneTreeiENSGT00820000127100.
HOGENOMiHOG000112147.
HOVERGENiHBG051297.
InParanoidiQ9UN19.
KOiK12229.
OMAiKVNCFCL.
OrthoDBiEOG091G0HCX.
PhylomeDBiQ9UN19.
TreeFamiTF105418.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UN19-1) [UniParc]FASTAAdd to basket
Also known as: hBam1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAELLEGK MSTQDPSDLW SRSDGEAELL QDLGWYHGNL TRHAAEALLL
60 70 80 90 100
SNGCDGSYLL RDSNETTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEFS
110 120 130 140 150
SLKDFVKHFA NQPLIGSETG TLMVLKHPYP RKVEEPSIYE SVRVHTAMQT
160 170 180 190 200
GRTEDDLVPT APSLGTKEGY LTKQGGLVKT WKTRWFTLHR NELKYFKDQM
210 220 230 240 250
SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC AKTGVEADEW
260 270 280
IKILRWKLSQ IRKQLNQGEG TIRSRSFIFK
Length:280
Mass (Da):32,194
Last modified:May 1, 2000 - v1
Checksum:i4A7F686B55146241
GO
Isoform 2 (identifier: Q9UN19-2) [UniParc]FASTAAdd to basket
Also known as: hBam2

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: SQIRKQLNQGEGTIRSRSFIFK → VKDKSCILSALCISPEEKTDHK

Show »
Length:280
Mass (Da):32,044
Checksum:i643DF0A3C4FB32DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216 – 229VQFDY…VNCFC → MICNILCSFFCPIS (PubMed:17974005).CuratedAdd BLAST14

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010699259 – 280SQIRK…SFIFK → VKDKSCILSALCISPEEKTD HK in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163254 mRNA. Translation: AAD49697.1.
AF161551 mRNA. Translation: AAF29038.1.
AF186022 mRNA. Translation: AAF14578.1.
AF178987 mRNA. Translation: AAF44351.1.
BC012924 mRNA. Translation: AAH12924.1.
AL713793 mRNA. Translation: CAD28547.1.
CCDSiCCDS47112.1. [Q9UN19-1]
RefSeqiNP_001293080.1. NM_001306151.1.
NP_055210.2. NM_014395.2. [Q9UN19-1]
UniGeneiHs.436271.
Hs.708484.

Genome annotation databases

EnsembliENST00000512369; ENSP00000423602; ENSG00000070190. [Q9UN19-1]
GeneIDi27071.
KEGGihsa:27071.
UCSCiuc003hvf.5. human. [Q9UN19-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163254 mRNA. Translation: AAD49697.1.
AF161551 mRNA. Translation: AAF29038.1.
AF186022 mRNA. Translation: AAF14578.1.
AF178987 mRNA. Translation: AAF44351.1.
BC012924 mRNA. Translation: AAH12924.1.
AL713793 mRNA. Translation: CAD28547.1.
CCDSiCCDS47112.1. [Q9UN19-1]
RefSeqiNP_001293080.1. NM_001306151.1.
NP_055210.2. NM_014395.2. [Q9UN19-1]
UniGeneiHs.436271.
Hs.708484.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAOX-ray1.80A148-273[»]
1FB8X-ray2.40A148-273[»]
ProteinModelPortaliQ9UN19.
SMRiQ9UN19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117981. 16 interactors.
IntActiQ9UN19. 18 interactors.
MINTiMINT-1499560.
STRINGi9606.ENSP00000423602.

PTM databases

iPTMnetiQ9UN19.
PhosphoSitePlusiQ9UN19.

Polymorphism and mutation databases

BioMutaiDAPP1.
DMDMi51317293.

Proteomic databases

PaxDbiQ9UN19.
PeptideAtlasiQ9UN19.
PRIDEiQ9UN19.

Protocols and materials databases

DNASUi27071.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000512369; ENSP00000423602; ENSG00000070190. [Q9UN19-1]
GeneIDi27071.
KEGGihsa:27071.
UCSCiuc003hvf.5. human. [Q9UN19-1]

Organism-specific databases

CTDi27071.
DisGeNETi27071.
GeneCardsiDAPP1.
H-InvDBHIX0004401.
HGNCiHGNC:16500. DAPP1.
HPAiCAB033822.
HPA046074.
MIMi605768. gene.
neXtProtiNX_Q9UN19.
OpenTargetsiENSG00000070190.
PharmGKBiPA27145.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF6C. Eukaryota.
ENOG410XSW5. LUCA.
GeneTreeiENSGT00820000127100.
HOGENOMiHOG000112147.
HOVERGENiHBG051297.
InParanoidiQ9UN19.
KOiK12229.
OMAiKVNCFCL.
OrthoDBiEOG091G0HCX.
PhylomeDBiQ9UN19.
TreeFamiTF105418.

Enzyme and pathway databases

ReactomeiR-HSA-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiQ9UN19.
SIGNORiQ9UN19.

Miscellaneous databases

EvolutionaryTraceiQ9UN19.
GeneWikiiDAPP1.
GenomeRNAii27071.
PROiQ9UN19.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000070190.
CleanExiHS_DAPP1.
ExpressionAtlasiQ9UN19. baseline and differential.
GenevisibleiQ9UN19. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAPP1_HUMAN
AccessioniPrimary (citable) accession number: Q9UN19
Secondary accession number(s): Q8TCK5, Q9UHF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.