Q9UMY4 (SNX12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorting nexin-12

Gene names

Name:

SNX12

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in several stages of intracellular trafficking By similarity.
Subcellular location	Membrane; Peripheral membrane protein; Cytoplasmic side Potential.
Domain	The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate By similarity.
Sequence similarities	Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylinositol binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	3	EBI-1752602,EBI-389883

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UMY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UMY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-172: SLAVSCPGWSAVA → VRQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 172

172

Sorting nexin-12

PRO_0000213858

Regions

Domain

28 – 152

125

PX

Sites

Binding site

71

1

Phosphatidylinositol 3-phosphate By similarity

Binding site

73

1

Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity

Binding site

96

1

Phosphatidylinositol 3-phosphate By similarity

Binding site

119

1

Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue

23

1

Phosphotyrosine Ref.4

Modified residue

73

1

Phosphoserine Ref.5 Ref.6

Natural variations

Alternative sequence

160 – 172

13

SLAVS…WSAVA → VRQ in isoform 2.

VSP_011038

Experimental info

Sequence conflict

101

1

Q → K in AAD48491. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

172

Helix Strand Turn

Details...

Beta strand

31 – 45

15

Beta strand

47 – 55

9

Beta strand

59 – 62

4

Beta strand

64 – 70

7

Helix

72 – 82

11

Beta strand

83 – 87

5

Helix

113 – 132

20

Helix

134 – 138

5

Helix

140 – 145

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 3. Checksum: A9221EB7A4CD5A29	FASTA	172	19,730
	10 20 30 40 50 60 MSDTAVADTR RLNSKPQDLT DAYGPPSNFL EIDIFNPQTV GVGRARFTTY EVRMRTNLPI 70 80 90 100 110 120 FKLKESCVRR RYSDFEWLKN ELERDSKIVV PPLPGKALKR QLPFRGDEGI FEESFIEERR 130 140 150 160 170 QGLEQFINKI AGHPLAQNER CLHMFLQEEA IDRNYVPGKS LAVSCPGWSA VA
	Isoform 2 [UniParc]. Checksum: 48CBC16A86AFDB08	FASTA	162	18,885
	10 20 30 40 50 60 MSDTAVADTR RLNSKPQDLT DAYGPPSNFL EIDIFNPQTV GVGRARFTTY EVRMRTNLPI 70 80 90 100 110 120 FKLKESCVRR RYSDFEWLKN ELERDSKIVV PPLPGKALKR QLPFRGDEGI FEESFIEERR 130 140 150 160 QGLEQFINKI AGHPLAQNER CLHMFLQEEA IDRNYVPGKV RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large family of endosome-localized proteins related to sorting nexin 1." Teasdale R.D., Loci D., Houghton F., Karlsson L., Gleeson P.A. Biochem. J. 358:7-16(2001) [PubMed: 11485546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[4]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-23, MASS SPECTROMETRY.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Solution structure of PX domain from human SNX12." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 27-160.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF171229 mRNA. Translation: AAD48491.1.
BT007203 mRNA. Translation: AAP35867.1.
BC020559 mRNA. No translation available.

IPI

IPI00438170.
IPI00844112.

RefSeq

NP_037478.2. NM_013346.2.

UniGene

Hs.260750.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CSK	NMR	-	A	27-159	[»]

ProteinModelPortal

Q9UMY4.

SMR

Q9UMY4. Positions 28-160.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UMY4. 4 interactions.

MINT

MINT-3082546.

STRING

Q9UMY4.

PTM databases

PhosphoSite

Q9UMY4.

Polymorphism databases

DMDM

50403807.

2D gel databases

UCD-2DPAGE

Q9UMY4.

Proteomic databases

PRIDE

Q9UMY4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000374281; ENSP00000363399; ENSG00000147164.

GeneID

29934.

KEGG

hsa:29934.

UCSC

uc004dyr.1. human.

Organism-specific databases

CTD

29934.

GeneCards

GC0XM070276.

H-InvDB

HIX0022439.

HGNC

HGNC:14976. SNX12.

neXtProt

NX_Q9UMY4.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00600000084448.

HOGENOM

HBG714933.

HOVERGEN

HBG055338.

InParanoid

Q9UMY4.

PhylomeDB

Q9UMY4.

Gene expression databases

ArrayExpress

Q9UMY4.

CleanEx

HS_SNX12.

Genevestigator

Q9UMY4.

GermOnline

ENSG00000147164. Homo sapiens.

Family and domain databases

InterPro

IPR001683. Phox.
[Graphical view]

Gene3D

G3DSA:3.30.1520.10. PX. 1 hit.

Pfam

PF00787. PX. 1 hit.
[Graphical view]

SMART

SM00312. PX. 1 hit.
[Graphical view]

SUPFAM

SSF64268. PX. 1 hit.

PROSITE

PS50195. PX. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

52575.

PMAP-CutDB

Q9UMY4.

Entry information

Entry name

SNX12_HUMAN

Accession

Primary (citable) accession number: Q9UMY4
Secondary accession number(s): Q8WUG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.