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Protein

Neudesin

Gene

NENF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neudesin
Alternative name(s):
Cell immortalization-related protein 2
Neuron-derived neurotrophic factor
Protein GIG471 Publication
Secreted protein of unknown function
Short name:
SPUF protein
Gene namesi
Name:NENF
Synonyms:CIR2, SPUF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:30384. NENF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671266.

Polymorphism and mutation databases

BioMutaiNENF.
DMDMi46577571.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 172141NeudesinPRO_0000018598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 1361N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9UMX5.
MaxQBiQ9UMX5.
PaxDbiQ9UMX5.
PRIDEiQ9UMX5.
TopDownProteomicsiQ9UMX5.

PTM databases

iPTMnetiQ9UMX5.
PhosphoSiteiQ9UMX5.

Expressioni

Tissue specificityi

Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.1 Publication

Inductioni

Up-regulated in immortal cells. Induced in estrogen receptor positive breast cancer expressing progesterone receptor.2 Publications

Gene expression databases

BgeeiQ9UMX5.
CleanExiHS_NENF.
GenevisibleiQ9UMX5. HS.

Organism-specific databases

HPAiHPA026763.
HPA028488.

Interactioni

Protein-protein interaction databases

BioGridi118976. 35 interactions.
IntActiQ9UMX5. 1 interaction.
STRINGi9606.ENSP00000355955.

Structurei

3D structure databases

ProteinModelPortaliQ9UMX5.
SMRiQ9UMX5. Positions 47-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 12986Cytochrome b5 heme-bindingAdd
BLAST

Domaini

The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.By similarity

Sequence similaritiesi

Belongs to the cytochrome b5 family. MAPR subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1110. Eukaryota.
ENOG4111UG0. LUCA.
GeneTreeiENSGT00730000110948.
HOGENOMiHOG000187840.
HOVERGENiHBG059971.
InParanoidiQ9UMX5.
OMAiGKGAPYN.
OrthoDBiEOG7K3TNZ.
PhylomeDBiQ9UMX5.
TreeFamiTF332131.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UMX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGPAPRRRL RPLAALALVL ALAPGLPTAR AGQTPRPAER GPPVRLFTEE
60 70 80 90 100
ELARYGGEEE DQPIYLAVKG VVFDVTSGKE FYGRGAPYNA LTGKDSTRGV
110 120 130 140 150
AKMSLDPADL THDTTGLTAK ELEALDEVFT KVYKAKYPIV GYTARRILNE
160 170
DGSPNLDFKP EDQPHFDIKD EF
Length:172
Mass (Da):18,856
Last modified:May 1, 2000 - v1
Checksum:i24527D540B000F2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741D → E in BAD96855 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB126219 mRNA. Translation: BAD72063.1.
AF173937 mRNA. Translation: AAD51419.1.
AY762102 mRNA. Translation: AAX07829.1.
AK223135 mRNA. Translation: BAD96855.1.
CH471100 Genomic DNA. Translation: EAW93389.1.
BC008823 mRNA. Translation: AAH08823.1.
CCDSiCCDS1505.1.
RefSeqiNP_037481.1. NM_013349.4.
UniGeneiHs.461787.

Genome annotation databases

EnsembliENST00000366988; ENSP00000355955; ENSG00000117691.
GeneIDi29937.
KEGGihsa:29937.
UCSCiuc001hjd.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB126219 mRNA. Translation: BAD72063.1.
AF173937 mRNA. Translation: AAD51419.1.
AY762102 mRNA. Translation: AAX07829.1.
AK223135 mRNA. Translation: BAD96855.1.
CH471100 Genomic DNA. Translation: EAW93389.1.
BC008823 mRNA. Translation: AAH08823.1.
CCDSiCCDS1505.1.
RefSeqiNP_037481.1. NM_013349.4.
UniGeneiHs.461787.

3D structure databases

ProteinModelPortaliQ9UMX5.
SMRiQ9UMX5. Positions 47-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118976. 35 interactions.
IntActiQ9UMX5. 1 interaction.
STRINGi9606.ENSP00000355955.

PTM databases

iPTMnetiQ9UMX5.
PhosphoSiteiQ9UMX5.

Polymorphism and mutation databases

BioMutaiNENF.
DMDMi46577571.

Proteomic databases

EPDiQ9UMX5.
MaxQBiQ9UMX5.
PaxDbiQ9UMX5.
PRIDEiQ9UMX5.
TopDownProteomicsiQ9UMX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366988; ENSP00000355955; ENSG00000117691.
GeneIDi29937.
KEGGihsa:29937.
UCSCiuc001hjd.3. human.

Organism-specific databases

CTDi29937.
GeneCardsiNENF.
HGNCiHGNC:30384. NENF.
HPAiHPA026763.
HPA028488.
MIMi611874. gene.
neXtProtiNX_Q9UMX5.
PharmGKBiPA142671266.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1110. Eukaryota.
ENOG4111UG0. LUCA.
GeneTreeiENSGT00730000110948.
HOGENOMiHOG000187840.
HOVERGENiHBG059971.
InParanoidiQ9UMX5.
OMAiGKGAPYN.
OrthoDBiEOG7K3TNZ.
PhylomeDBiQ9UMX5.
TreeFamiTF332131.

Miscellaneous databases

GenomeRNAii29937.
NextBioi52583.
PROiQ9UMX5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UMX5.
CleanExiHS_NENF.
GenevisibleiQ9UMX5. HS.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity."
    Kimura I., Yoshioka M., Konishi M., Miyake A., Itoh N.
    J. Neurosci. Res. 79:287-294(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Identification of a secreted protein of unknown function."
    Rasheed Z.A., Haluska P., Saleem A., Rubin E.H.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Identification of a cell growth-inhibiting gene."
    Kim J.W.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastric carcinoma.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  7. "Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines."
    Ma L., Broomfield S., Lavery C., Lin S.L., Xiao W., Bacchetti S.
    Oncogene 17:1321-1326(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Breast cancer proteomics by laser capture microdissection, sample pooling, 54-cm IPG IEF, and differential iodine radioisotope detection."
    Neubauer H., Clare S.E., Kurek R., Fehm T., Wallwiener D., Sotlar K., Nordheim A., Wozny W., Schwall G.P., Poznanovic S., Sastri C., Hunzinger C., Stegmann W., Schrattenholz A., Cahill M.A.
    Electrophoresis 27:1840-1852(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The functional and structural characterization of a novel oncogene GIG47 involved in the breast tumorigenesis."
    Han K.H., Lee S.H., Ha S.A., Kim H.K., Lee C., Kim D.H., Gong K.H., Yoo J., Kim S., Kim J.W.
    BMC Cancer 12:274-274(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, STRUCTURE BY NMR.
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNENF_HUMAN
AccessioniPrimary (citable) accession number: Q9UMX5
Secondary accession number(s): A1KYQ8, Q53FZ6, Q5TM90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.