Q9UMQ6 (CAN11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-11
EC=3.4.22.-

Alternative name(s):
Calcium-activated neutral proteinase 11
Short name=CANP 11

Gene names

Name:

CAPN11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Catalytic activity	Broad endopeptidase specificity.
Subunit structure	Heterodimer of a large (catalytic) and a small (regulatory) subunit.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › acrosome By similarity.
Tissue specificity	Highest expression in testis. Ref.5
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 2 EF-hand domains.
Sequence caution	The sequence AAH33733.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG36761.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB52137.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent cysteine-type endopeptidase activity Traceable author statement Ref.5. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 739	739	Calpain-11		PRO_0000207729
Regions
	Domain	79 – 378	300	Calpain catalytic
	Domain	610 – 643	34	EF-hand 1
	Domain	640 – 675	36	EF-hand 2
	Calcium binding	623 – 634	12	1 Potential
	Calcium binding	653 – 664	12	2 Potential
	Region	379 – 551	173	Domain III
	Region	552 – 567	16	Linker
	Region	568 – 738	171	Domain IV
Sites
	Active site	139	1	By similarity
	Active site	296	1	By similarity
	Active site	320	1	By similarity
Natural variations
	Natural variant	266	1	V → M. Corresponds to variant rs6938938 [ dbSNP | Ensembl ].		VAR_033713
	Natural variant	441	1	V → A. Ref.4 Corresponds to variant rs16871612 [ dbSNP | Ensembl ].		VAR_033714
	Natural variant	521	1	I → V. Corresponds to variant rs34710081 [ dbSNP | Ensembl ].		VAR_033715
	Natural variant	544	1	S → R. Corresponds to variant rs35527493 [ dbSNP | Ensembl ].		VAR_033716
	Natural variant	728	1	S → N. Corresponds to variant rs7761137 [ dbSNP | Ensembl ].		VAR_024587

Sequences

	Sequence		Length	Mass (Da)
	Q9UMQ6 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 8B102AF286259EFB	FASTA	739	84,423
	10 20 30 40 50 60 MLYSPGPSLP ESAESLDGSQ EDKPRGSCAE PTFTDTGMVA HINNSRLKAK GVGQHDNAQN 70 80 90 100 110 120 FGNQSFEELR AACLRKGELF EDPLFPAEPS SLGFKDLGPN SKNVQNISWQ RPKDIINNPL 130 140 150 160 170 180 FIMDGISPTD ICQGILGDCW LLAAIGSLTT CPKLLYRVVP RGQSFKKNYA GIFHFQIWQF 190 200 210 220 230 240 GQWVNVVVDD RLPTKNDKLV FVHSTERSEF WSALLEKAYA KLSGSYEALS GGSTMEGLED 250 260 270 280 290 300 FTGGVAQSFQ LQRPPQNLLR LLRKAVERSS LMGCSIEVTS DSELESMTDK MLVRGHAYSV 310 320 330 340 350 360 TGLQDVHYRG KMETLIRVRN PWGRIEWNGA WSDSAREWEE VASDIQMQLL HKTEDGEFWM 370 380 390 400 410 420 SYQDFLNNFT LLEICNLTPD TLSGDYKSYW HTTFYEGSWR RGSSAGGCRN HPGTFWTNPQ 430 440 450 460 470 480 FKISLPEGDD PEDDAEGNVV VCTCLVALMQ KNWRHARQQG AQLQTIGFVL YAVPKEFQNI 490 500 510 520 530 540 QDVHLKKEFF TKYQDHGFSE IFTNSREVSS QLRLPPGEYI IIPSTFEPHR DADFLLRVFT 550 560 570 580 590 600 EKHSESWELD EVNYAEQLQE EKVSEDDMDQ DFLHLFKIVA GEGKEIGVYE LQRLLNRMAI 610 620 630 640 650 660 KFKSFKTKGF GLDACRCMIN LMDKDGSGKL GLLEFKILWK KLKKWMDIFR ECDQDHSGTL 670 680 690 700 710 720 NSYEMRLVIE KAGIKLNNKV MQVLVARYAD DDLIIDFDSF ISCFLRLKTM FTFFLTMDPK 730 NTGHICLSLE QWLQMTMWG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-441. Tissue: Brain.
[5]	"CAPN11: a calpain with high mRNA levels in testis and located on chromosome 6." Dear T.N., Moller A., Boehm T. Genomics 59:243-247(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-739, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK314054 mRNA. Translation: BAG36761.1. Different initiation. AL365192 Genomic DNA. Translation: CAI20532.2. CH471081 Genomic DNA. Translation: EAX04250.1. BC033733 mRNA. Translation: AAH33733.1. Different initiation. AJ242832 mRNA. Translation: CAB52137.1. Different initiation.
IPI	IPI00218934.
RefSeq	NP_008989.2. NM_007058.3.
UniGene	Hs.225953.
3D structure databases
ProteinModelPortal	Q9UMQ6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UMQ6. 4 interactions.
STRING	9606.ENSP00000381758.
Protein family/group databases
MEROPS	C02.013.
PTM databases
PhosphoSite	Q9UMQ6.
Polymorphism databases
DMDM	218511961.
Proteomic databases
PaxDb	Q9UMQ6.
PRIDE	Q9UMQ6.
Protocols and materials databases
DNASU	11131.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000398776; ENSP00000381758; ENSG00000137225.
GeneID	11131.
KEGG	hsa:11131.
UCSC	uc003owt.1. human.
Organism-specific databases
CTD	11131.
GeneCards	GC06P044126.
H-InvDB	HIX0200891.
HGNC	HGNC:1478. CAPN11.
HPA	CAB018070.
MIM	604822. gene.
neXtProt	NX_Q9UMQ6.
PharmGKB	PA26059.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG327523.
HOGENOM	HOG000232035.
HOVERGEN	HBG012645.
InParanoid	Q9UMQ6.
KO	K08580.
OMA	VCTCLVA.
OrthoDB	EOG4RR6GS.
Gene expression databases
ArrayExpress	Q9UMQ6.
Bgee	Q9UMQ6.
CleanEx	HS_CAPN11.
Genevestigator	Q9UMQ6.
GermOnline	ENSG00000137225. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
SUPFAM	SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11131.
NextBio	42308.
SOURCE	Search...

Entry information

Entry name

CAN11_HUMAN

Accession

Primary (citable) accession number: Q9UMQ6
Secondary accession number(s): B2RA64, Q5T3G1, Q8N4R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.