Q9UM63 (PLAL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein PLAGL1

Alternative name(s):
Lost on transformation 1
Short name=LOT-1
Pleiomorphic adenoma-like protein 1
Tumor supressor ZAC

Gene names

Name:	PLAGL1
Synonyms:	LOT1, ZAC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. Ref.11
Subunit structure	Interacts with THRSP. Ref.11
Subcellular location	Nucleus Ref.4 Ref.11.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Traceable author statement PubMed 9184226. Source: ProtInc cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome induction of apoptosis Traceable author statement Ref.3. Source: ProtInc positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15888726. Source: MGI
Cellular_component	nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	DNA binding Inferred from direct assay PubMed 15888725. Source: MGI sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UM63-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UM63-2)

Also known as: ZACdelta2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Zinc finger protein PLAGL1		PRO_0000047222
Regions
	Zinc finger	4 – 26	23	C2H2-type 1
	Zinc finger	32 – 56	25	C2H2-type 2
	Zinc finger	62 – 84	23	C2H2-type 3
	Zinc finger	91 – 113	23	C2H2-type 4
	Zinc finger	120 – 142	23	C2H2-type 5
	Zinc finger	156 – 178	23	C2H2-type 6
	Zinc finger	184 – 207	24	C2H2-type 7
Natural variations
	Alternative sequence	1 – 52	52	Missing in isoform 2.		VSP_028123
	Natural variant	272	1	A → V. Corresponds to variant rs35263016 [ dbSNP | Ensembl ].		VAR_052729
Experimental info
	Sequence conflict	81	1	L → F in AAB67041. Ref.1
	Sequence conflict	197	1	D → V in AAC34250. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: B10C74DA409BD4F7	FASTA	463	50,819
	10 20 30 40 50 60 MATFPCQLCG KTFLTLEKFT IHNYSHSRER PYKCVQPDCG KAFVSRYKLM RHMATHSPQK 70 80 90 100 110 120 SHQCAHCEKT FNRKDHLKNH LQTHDPNKMA FGCEECGKKY NTMLGYKRHL ALHAASSGDL 130 140 150 160 170 180 TCGVCALELG STEVLLDHLK AHAEEKPPSG TKEKKHQCDH CERCFYTRKD VRRHLVVHTG 190 200 210 220 230 240 CKDFLCQFCA QRFGRKDHLT RHTKKTHSQE LMKESLQTGD LLSTFHTISP SFQLKAAALP 250 260 270 280 290 300 PFPLGASAQN GLASSLPAEV HSLTLSPPEQ AAQPMQPLPE SLASLHPSVS PGSPPPPLPN 310 320 330 340 350 360 HKYNTTSTSY SPLASLPLKA DTKGFCNISL FEDLPLQEPQ SPQKLNPGFD LAKGNAGKVN 370 380 390 400 410 420 LPKELPADAV NLTIPASLDL SPLLGFWQLP PPATQNTFGN STLALGPGES LPHRLSCLGQ 430 440 450 460 QQQEPPLAMG TVSLGQLPLP PIPHVFSAGT GSAILPHFHH AFR
	Isoform 2 (ZACdelta2) [UniParc]. Checksum: 735121F4BF16F7C8	FASTA	411	44,668
	10 20 30 40 50 60 MATHSPQKSH QCAHCEKTFN RKDHLKNHLQ THDPNKMAFG CEECGKKYNT MLGYKRHLAL 70 80 90 100 110 120 HAASSGDLTC GVCALELGST EVLLDHLKAH AEEKPPSGTK EKKHQCDHCE RCFYTRKDVR 130 140 150 160 170 180 RHLVVHTGCK DFLCQFCAQR FGRKDHLTRH TKKTHSQELM KESLQTGDLL STFHTISPSF 190 200 210 220 230 240 QLKAAALPPF PLGASAQNGL ASSLPAEVHS LTLSPPEQAA QPMQPLPESL ASLHPSVSPG 250 260 270 280 290 300 SPPPPLPNHK YNTTSTSYSP LASLPLKADT KGFCNISLFE DLPLQEPQSP QKLNPGFDLA 310 320 330 340 350 360 KGNAGKVNLP KELPADAVNL TIPASLDLSP LLGFWQLPPP ATQNTFGNST LALGPGESLP 370 380 390 400 410 HRLSCLGQQQ QEPPLAMGTV SLGQLPLPPI PHVFSAGTGS AILPHFHHAF R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a zinc-finger gene at 6q25: a chromosomal region implicated in development of many solid tumors." Abdollahi A., Roberts D., Godwin A.K., Schultz D.C., Sonoda G., Testa J.R., Hamilton T.C. Oncogene 14:1973-1979(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Transcriptional activation capacity of the novel PLAG family of zinc finger proteins." Kas K., Voz M.L., Hensen K., Meyen E., Van de Ven W.J.M. J. Biol. Chem. 273:23026-23032(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"hZAC encodes a zinc finger protein with antiproliferative properties and maps to a chromosomal region frequently lost in cancer." Varrault A., Ciani E., Apiou F., Bilanges B., Hoffmann A., Pantaloni C., Bockaert J., Spengler D., Journot L. Proc. Natl. Acad. Sci. U.S.A. 95:8835-8840(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Pituitary.
[4]	"Alternative splicing of the imprinted candidate tumor suppressor gene ZAC regulates its antiproliferative and DNA binding activities." Bilanges B., Varrault A., Mazumdar A., Pantaloni C., Hoffmann A., Bockaert J., Spengler D., Journot L. Oncogene 20:1246-1253(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION. Tissue: Mammary gland.
[5]	"Characterization of the methylation-sensitive promoter of the imprinted ZAC (PLAGL1) gene supports its role in transient neonatal diabetes mellitus." Varrault A., Bilanges B., Mackay D.J., Basyuk E., Ahr B., Fernandez C., Robinson D.O., Bockaert J., Journot L. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Colon.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Fetal kidney.
[8]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[11]	"Human Spot 14 protein is a p53-dependent transcriptional coactivator via the recruitment of thyroid receptor and Zac1." Chou W.Y., Ho C.L., Tseng M.L., Liu S.T., Yen L.C., Huang S.M. Int. J. Biochem. Cell Biol. 40:1826-1834(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THRSP.
+	Additional computationally mapped references.

Web resources

GeneReviews

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72621 mRNA. Translation: AAB67041.1. U81992 mRNA. Translation: AAC34250.1. AJ006354 mRNA. Translation: CAA06994.1. AJ303119 mRNA. Translation: CAC39614.1. AJ311395 mRNA. Translation: CAC39615.1. AK314570 mRNA. Translation: BAG37151.1. AK315184 mRNA. Translation: BAG37625.1. BX537397 mRNA. Translation: CAD97639.1. AL049844 Genomic DNA. Translation: CAI21447.1. AL049844 Genomic DNA. Translation: CAB56564.1. CH471051 Genomic DNA. Translation: EAW47852.1. CH471051 Genomic DNA. Translation: EAW47853.1. CH471051 Genomic DNA. Translation: EAW47854.1. CH471051 Genomic DNA. Translation: EAW47855.1. CH471051 Genomic DNA. Translation: EAW47856.1. BC074814 mRNA. Translation: AAH74814.1. BC109085 mRNA. Translation: AAI09086.1. BC109086 mRNA. Translation: AAI09087.1.
IPI	IPI00220818. IPI00645881.
RefSeq	NP_001074420.1. NM_001080951.1. NP_001074421.1. NM_001080952.1. NP_001074422.1. NM_001080953.1. NP_001074423.1. NM_001080954.1. NP_001074424.1. NM_001080955.1. NP_001074425.1. NM_001080956.1. NP_002647.2. NM_002656.3. NP_006709.2. NM_006718.3.
UniGene	Hs.444975.
3D structure databases
ProteinModelPortal	Q9UM63.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1188459.
STRING	9606.ENSP00000346810.
PTM databases
PhosphoSite	Q9UM63.
Polymorphism databases
DMDM	158523334.
Proteomic databases
PaxDb	Q9UM63.
PRIDE	Q9UM63.
Protocols and materials databases
DNASU	5325.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354765; ENSP00000346810; ENSG00000118495. ENST00000360537; ENSP00000353734; ENSG00000118495. ENST00000367571; ENSP00000356543; ENSG00000118495. ENST00000367572; ENSP00000356544; ENSG00000118495. ENST00000392307; ENSP00000376124; ENSG00000118495. ENST00000392309; ENSP00000376125; ENSG00000118495. ENST00000416623; ENSP00000400060; ENSG00000118495. ENST00000429150; ENSP00000398409; ENSG00000118495. ENST00000437412; ENSP00000392418; ENSG00000118495. ENST00000444202; ENSP00000400929; ENSG00000118495.
GeneID	5325.
KEGG	hsa:5325.
UCSC	uc003qjv.3. human.
Organism-specific databases
CTD	5325.
GeneCards	GC06M144261.
HGNC	HGNC:9046. PLAGL1.
MIM	603044. gene.
neXtProt	NX_Q9UM63.
Orphanet	96191. Paternal uniparental disomy of chromosome 6.
PharmGKB	PA33379.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOVERGEN	HBG053608.
InParanoid	Q9UM63.
OMA	TCGVCAL.
OrthoDB	EOG48WC1V.
PhylomeDB	Q9UM63.
Gene expression databases
ArrayExpress	Q9UM63.
Bgee	Q9UM63.
CleanEx	HS_PLAGL1.
Genevestigator	Q9UM63.
GermOnline	ENSG00000118495. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 5 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 7 hits. PS50157. ZINC_FINGER_C2H2_2. 7 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PLAGL1. human.
GenomeRNAi	5325.
NextBio	20600.
SOURCE	Search...

Entry information

Entry name

PLAL1_HUMAN

Accession

Primary (citable) accession number: Q9UM63
Secondary accession number(s): B2RBA4 , B2RCM8, E1P595, E1P597, O76019, Q7Z3V8, Q92981, Q96JR9, Q9UIZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.