Q9UM21 (MGT4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.145

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name=GlcNAc-T IVa
Short name=GnT-IVa
Short name=N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa

Cleaved into the following chain:

1. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form

Gene names

Name:

MGAT4A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted By similarity.
Tissue specificity	Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 54 family.
Biophysicochemical properties	Kinetic parameters: KM=0.118 mM for UDP-GlcNAc Ref.9

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Traceable author statement Ref.1. Source: ProtInc post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Traceable author statement Ref.1. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UM21-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UM21-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRLRNG → MNYSIT
     7-134: Missing.
     528-535: IHIKKATN → QRVFSGWWQKSRDKAEGPQAPLLF

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A		PRO_0000288581
	Chain	93 – 535	443	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form By similarity		PRO_0000288582
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 27	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 535	508	Lumenal Potential
	Coiled coil	28 – 63	36	Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 6	6	MRLRNG → MNYSIT in isoform 2.		VSP_046075
	Alternative sequence	7 – 134	128	Missing in isoform 2.		VSP_046076
	Alternative sequence	528 – 535	8	IHIKKATN → QRVFSGWWQKSRDKAEGPQA PLLF in isoform 2.		VSP_046077
Experimental info
	Sequence conflict	280	1	L → F in BAG65228. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C139E5F323A083A1	FASTA	535	61,544
	10 20 30 40 50 60 MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI AEHRISQRSS 70 80 90 100 110 120 ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK KSLQVPSIYY HLPHLLKNEG 130 140 150 160 170 180 SLQPAVQIGN GRTGVSIVMG IPTVKREVKS YLIETLHSLI DNLYPEEKLD CVIVVFIGET 190 200 210 220 230 240 DIDYVHGVVA NLEKEFSKEI SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL 250 260 270 280 290 300 DYCFLMMYAQ EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF 310 320 330 340 350 360 QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL RIRFRPSLFQ 370 380 390 400 410 420 HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL KVYQGHTLEK TYMGEDFFWA 430 440 450 460 470 480 ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE HPGDILLNTT VEVLPFKSEG LEISKETKDK 490 500 510 520 530 RLEDGYFRIG KFENGVAEGM VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATN
	Isoform 2 [UniParc]. Checksum: 5267882303493B59	FASTA	423	48,946
	10 20 30 40 50 60 MNYSITVSIV MGIPTVKREV KSYLIETLHS LIDNLYPEEK LDCVIVVFIG ETDIDYVHGV 70 80 90 100 110 120 VANLEKEFSK EISSGLVEVI SPPESYYPDL TNLKETFGDS KERVRWRTKQ NLDYCFLMMY 130 140 150 160 170 180 AQEKGIYYIQ LEDDIIVKQN YFNTIKNFAL QLSSEEWMIL EFSQLGFIGK MFQAPDLTLI 190 200 210 220 230 240 VEFIFMFYKE KPIDWLLDHI LWVKVCNPEK DAKHCDRQKA NLRIRFRPSL FQHVGLHSSL 250 260 270 280 290 300 SGKIQKLTDK DYMKPLLLKI HVNPPAEVST SLKVYQGHTL EKTYMGEDFF WAITPIAGDY 310 320 330 340 350 360 ILFKFDKPVN VESYLFHSGN QEHPGDILLN TTVEVLPFKS EGLEISKETK DKRLEDGYFR 370 380 390 400 410 420 IGKFENGVAE GMVDPSLNPI SAFRLSVIQN SAVWAILNEQ RVFSGWWQKS RDKAEGPQAP LLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase." Yoshida A., Minowa M.T., Takamatsu S., Hara T., Oguri S., Ikenaga H., Takeuchi M. Glycobiology 9:303-310(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Trachea.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Alternative splicing in human N-acetylglucosaminyltransferase IVa gene." Minowa M.T., Takeuchi M. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
[7]	"Unusually high expression of N-acetylglucosaminyltransferase-IVa in human choriocarcinoma cell lines: a possible enzymatic basis of the formation of abnormal biantennary sugar chain." Takamatsu S., Oguri S., Minowa M.T., Yoshida A., Nakamura K., Takeuchi M., Kobata A. Cancer Res. 59:3949-3953(1999) [PubMed] [Europe PMC] [Abstract] Cited for: OVEREXPRESSION IN CHORIOCARCINOMA.
[8]	"Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in pancreatic cancer." Ide Y., Miyoshi E., Nakagawa T., Gu J., Tanemura M., Nishida T., Ito T., Yamamoto H., Kozutsumi Y., Taniguchi N. Biochem. Biophys. Res. Commun. 341:478-482(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DOWN-REGULATION IN PANCREATIC CANCER.
[9]	"Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes." Oguri S., Yoshida A., Minowa M.T., Takeuchi M. Glycoconj. J. 23:473-480(2006) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000616 mRNA. Translation: BAA86388.1. AK304393 mRNA. Translation: BAG65228.1. AC064860 Genomic DNA. Translation: AAY14976.1. AC109826 Genomic DNA. Translation: AAY24092.1. CH471127 Genomic DNA. Translation: EAX01896.1. CH471127 Genomic DNA. Translation: EAX01897.1. BC127107 mRNA. Translation: AAI27108.1. BC127108 mRNA. Translation: AAI27109.1. AB053222 Genomic DNA. Translation: BAC55023.1.
IPI	IPI00016743. IPI00908866.
RefSeq	NP_001153626.1. NM_001160154.1. NP_036346.1. NM_012214.2.
UniGene	Hs.177576.
3D structure databases
ProteinModelPortal	Q9UM21.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000264968.
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
PTM databases
PhosphoSite	Q9UM21.
Polymorphism databases
DMDM	74721048.
Proteomic databases
PaxDb	Q9UM21.
PRIDE	Q9UM21.
Protocols and materials databases
DNASU	11320.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264968; ENSP00000264968; ENSG00000071073. ENST00000393487; ENSP00000377127; ENSG00000071073. ENST00000409391; ENSP00000386841; ENSG00000071073. ENST00000414521; ENSP00000404889; ENSG00000071073.
GeneID	11320.
KEGG	hsa:11320.
UCSC	uc002sze.3. human.
Organism-specific databases
CTD	11320.
GeneCards	GC02M099236.
HGNC	HGNC:7047. MGAT4A.
HPA	HPA007608.
MIM	604623. gene.
neXtProt	NX_Q9UM21.
PharmGKB	PA30782.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG78968.
HOGENOM	HOG000015085.
HOVERGEN	HBG053194.
InParanoid	Q9UM21.
KO	K00738.
OMA	DFFWAVT.
OrthoDB	EOG428219.
PhylomeDB	Q9UM21.
Enzyme and pathway databases
BRENDA	2.4.1.145. 2681.
Reactome	REACT_17015. Metabolism of proteins.
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q9UM21.
Bgee	Q9UM21.
CleanEx	HS_MGAT4A.
Genevestigator	Q9UM21.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. PTHR12062. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11320.
NextBio	43007.
SOURCE	Search...

Entry information

Entry name

MGT4A_HUMAN

Accession

Primary (citable) accession number: Q9UM21
Secondary accession number(s): B4E2R6 , D3DVH6, E9PEN2, Q53S97, Q86Z15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.