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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Cofactori

Kineticsi

  1. KM=0.118 mM for UDP-GlcNAc1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    LigandMetal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS01024-MONOMER
    BRENDAi2.4.1.145 2681
    ReactomeiR-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
    R-HSA-8957275 Post-translational protein phosphorylation
    R-HSA-975577 N-Glycan antennae elongation
    UniPathwayiUPA00378

    Protein family/group databases

    CAZyiGT54 Glycosyltransferase Family 54

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
    Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
    Short name:
    GlcNAc-T IVa
    Short name:
    GnT-IVa
    Short name:
    N-acetylglucosaminyltransferase IVa
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    Cleaved into the following chain:
    Gene namesi
    Name:MGAT4A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000071073.12
    HGNCiHGNC:7047 MGAT4A
    MIMi604623 gene
    neXtProtiNX_Q9UM21

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 4CytoplasmicSequence analysis4
    Transmembranei5 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
    Topological domaini28 – 535LumenalSequence analysisAdd BLAST508

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi11320
    OpenTargetsiENSG00000071073
    PharmGKBiPA30782

    Polymorphism and mutation databases

    BioMutaiMGAT4A

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002885811 – 535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase AAdd BLAST535
    ChainiPRO_000028858293 – 535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityAdd BLAST443

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
    Modified residuei474Phosphoserine; by FAM20C1 Publication1

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ9UM21
    PaxDbiQ9UM21
    PeptideAtlasiQ9UM21
    PRIDEiQ9UM21

    PTM databases

    iPTMnetiQ9UM21
    PhosphoSitePlusiQ9UM21

    Expressioni

    Tissue specificityi

    Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer.1 Publication

    Gene expression databases

    BgeeiENSG00000071073
    CleanExiHS_MGAT4A
    ExpressionAtlasiQ9UM21 baseline and differential
    GenevisibleiQ9UM21 HS

    Organism-specific databases

    HPAiHPA007608

    Interactioni

    Protein-protein interaction databases

    BioGridi116451, 1 interactor
    STRINGi9606.ENSP00000264968

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UM21
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili28 – 63Sequence analysisAdd BLAST36

    Sequence similaritiesi

    Belongs to the glycosyltransferase 54 family.Curated

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IEDC Eukaryota
    ENOG410XPV0 LUCA
    GeneTreeiENSGT00550000074578
    HOGENOMiHOG000015085
    HOVERGENiHBG053194
    InParanoidiQ9UM21
    KOiK00738
    OMAiSSEDWMI
    OrthoDBiEOG091G06KB
    PhylomeDBiQ9UM21
    TreeFamiTF324570

    Family and domain databases

    InterProiView protein in InterPro
    IPR006759 Glyco_transf_54
    PANTHERiPTHR12062 PTHR12062, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9UM21-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI
    60 70 80 90 100
    AEHRISQRSS ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK
    110 120 130 140 150
    KSLQVPSIYY HLPHLLKNEG SLQPAVQIGN GRTGVSIVMG IPTVKREVKS
    160 170 180 190 200
    YLIETLHSLI DNLYPEEKLD CVIVVFIGET DIDYVHGVVA NLEKEFSKEI
    210 220 230 240 250
    SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
    260 270 280 290 300
    EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
    310 320 330 340 350
    QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
    360 370 380 390 400
    RIRFRPSLFQ HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL
    410 420 430 440 450
    KVYQGHTLEK TYMGEDFFWA ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE
    460 470 480 490 500
    HPGDILLNTT VEVLPFKSEG LEISKETKDK RLEDGYFRIG KFENGVAEGM
    510 520 530
    VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATN
    Length:535
    Mass (Da):61,544
    Last modified:May 1, 2000 - v1
    Checksum:iC139E5F323A083A1
    GO
    Isoform 2 (identifier: Q9UM21-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: MRLRNG → MNYSIT
         7-134: Missing.
         528-535: IHIKKATN → QRVFSGWWQKSRDKAEGPQAPLLF

    Note: No experimental confirmation available.
    Show »
    Length:423
    Mass (Da):48,946
    Checksum:i5267882303493B59
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti280L → F in BAG65228 (PubMed:14702039).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0460751 – 6MRLRNG → MNYSIT in isoform 2. 1 Publication6
    Alternative sequenceiVSP_0460767 – 134Missing in isoform 2. 1 PublicationAdd BLAST128
    Alternative sequenceiVSP_046077528 – 535IHIKKATN → QRVFSGWWQKSRDKAEGPQA PLLF in isoform 2. 1 Publication8

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB000616 mRNA Translation: BAA86388.1
    AK304393 mRNA Translation: BAG65228.1
    AC064860 Genomic DNA Translation: AAY14976.1
    AC109826 Genomic DNA Translation: AAY24092.1
    CH471127 Genomic DNA Translation: EAX01896.1
    CH471127 Genomic DNA Translation: EAX01897.1
    BC127107 mRNA Translation: AAI27108.1
    BC127108 mRNA Translation: AAI27109.1
    AB053222 Genomic DNA Translation: BAC55023.1
    CCDSiCCDS2036.1 [Q9UM21-1]
    CCDS54380.1 [Q9UM21-2]
    RefSeqiNP_001153626.1, NM_001160154.1 [Q9UM21-2]
    NP_036346.1, NM_012214.2 [Q9UM21-1]
    UniGeneiHs.177576

    Genome annotation databases

    EnsembliENST00000264968; ENSP00000264968; ENSG00000071073 [Q9UM21-1]
    ENST00000393487; ENSP00000377127; ENSG00000071073 [Q9UM21-1]
    ENST00000409391; ENSP00000386841; ENSG00000071073 [Q9UM21-1]
    ENST00000414521; ENSP00000404889; ENSG00000071073 [Q9UM21-2]
    GeneIDi11320
    KEGGihsa:11320
    UCSCiuc002sze.3 human [Q9UM21-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiMGT4A_HUMAN
    AccessioniPrimary (citable) accession number: Q9UM21
    Secondary accession number(s): B4E2R6
    , D3DVH6, E9PEN2, Q53S97, Q86Z15
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 1, 2000
    Last modified: May 23, 2018
    This is version 126 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families
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    Main funding by: National Institutes of Health