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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Kineticsi

  1. KM=0.118 mM for UDP-GlcNAc1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    • N-glycan processing Source: ProtInc
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS01024-MONOMER.
    BRENDAi2.4.1.145. 2681.
    ReactomeiR-HSA-975577. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT54. Glycosyltransferase Family 54.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
    Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
    Short name:
    GlcNAc-T IVa
    Short name:
    GnT-IVa
    Short name:
    N-acetylglucosaminyltransferase IVa
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    Cleaved into the following chain:
    Gene namesi
    Name:MGAT4A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:7047. MGAT4A.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence analysis
    Transmembranei5 – 2723Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
    BLAST
    Topological domaini28 – 535508LumenalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • extracellular exosome Source: UniProtKB
    • Golgi membrane Source: Reactome
    • integral component of membrane Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30782.

    Polymorphism and mutation databases

    BioMutaiMGAT4A.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288581Add
    BLAST
    Chaini93 – 535443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityPRO_0000288582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
    Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis
    Modified residuei474 – 4741Phosphoserine; by FAM20C1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ9UM21.
    MaxQBiQ9UM21.
    PaxDbiQ9UM21.
    PeptideAtlasiQ9UM21.
    PRIDEiQ9UM21.

    PTM databases

    iPTMnetiQ9UM21.
    PhosphoSiteiQ9UM21.

    Expressioni

    Tissue specificityi

    Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer.1 Publication

    Gene expression databases

    BgeeiENSG00000071073.
    CleanExiHS_MGAT4A.
    ExpressionAtlasiQ9UM21. baseline and differential.
    GenevisibleiQ9UM21. HS.

    Organism-specific databases

    HPAiHPA007608.

    Interactioni

    Protein-protein interaction databases

    BioGridi116451. 1 interaction.
    STRINGi9606.ENSP00000264968.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UM21.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili28 – 6336Sequence analysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 54 family.Curated

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IEDC. Eukaryota.
    ENOG410XPV0. LUCA.
    GeneTreeiENSGT00550000074578.
    HOGENOMiHOG000015085.
    HOVERGENiHBG053194.
    InParanoidiQ9UM21.
    KOiK00738.
    OMAiLQVPNIY.
    OrthoDBiEOG091G06KB.
    PhylomeDBiQ9UM21.
    TreeFamiTF324570.

    Family and domain databases

    InterProiIPR006759. Glyco_transf_54.
    [Graphical view]
    PANTHERiPTHR12062. PTHR12062. 1 hit.
    PfamiPF04666. Glyco_transf_54. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9UM21-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI
    60 70 80 90 100
    AEHRISQRSS ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK
    110 120 130 140 150
    KSLQVPSIYY HLPHLLKNEG SLQPAVQIGN GRTGVSIVMG IPTVKREVKS
    160 170 180 190 200
    YLIETLHSLI DNLYPEEKLD CVIVVFIGET DIDYVHGVVA NLEKEFSKEI
    210 220 230 240 250
    SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
    260 270 280 290 300
    EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
    310 320 330 340 350
    QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
    360 370 380 390 400
    RIRFRPSLFQ HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL
    410 420 430 440 450
    KVYQGHTLEK TYMGEDFFWA ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE
    460 470 480 490 500
    HPGDILLNTT VEVLPFKSEG LEISKETKDK RLEDGYFRIG KFENGVAEGM
    510 520 530
    VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATN
    Length:535
    Mass (Da):61,544
    Last modified:May 1, 2000 - v1
    Checksum:iC139E5F323A083A1
    GO
    Isoform 2 (identifier: Q9UM21-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: MRLRNG → MNYSIT
         7-134: Missing.
         528-535: IHIKKATN → QRVFSGWWQKSRDKAEGPQAPLLF

    Note: No experimental confirmation available.
    Show »
    Length:423
    Mass (Da):48,946
    Checksum:i5267882303493B59
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti280 – 2801L → F in BAG65228 (PubMed:14702039).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 66MRLRNG → MNYSIT in isoform 2. 1 PublicationVSP_046075
    Alternative sequencei7 – 134128Missing in isoform 2. 1 PublicationVSP_046076Add
    BLAST
    Alternative sequencei528 – 5358IHIKKATN → QRVFSGWWQKSRDKAEGPQA PLLF in isoform 2. 1 PublicationVSP_046077

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB000616 mRNA. Translation: BAA86388.1.
    AK304393 mRNA. Translation: BAG65228.1.
    AC064860 Genomic DNA. Translation: AAY14976.1.
    AC109826 Genomic DNA. Translation: AAY24092.1.
    CH471127 Genomic DNA. Translation: EAX01896.1.
    CH471127 Genomic DNA. Translation: EAX01897.1.
    BC127107 mRNA. Translation: AAI27108.1.
    BC127108 mRNA. Translation: AAI27109.1.
    AB053222 Genomic DNA. Translation: BAC55023.1.
    CCDSiCCDS2036.1. [Q9UM21-1]
    CCDS54380.1. [Q9UM21-2]
    RefSeqiNP_001153626.1. NM_001160154.1. [Q9UM21-2]
    NP_036346.1. NM_012214.2. [Q9UM21-1]
    UniGeneiHs.177576.

    Genome annotation databases

    EnsembliENST00000264968; ENSP00000264968; ENSG00000071073. [Q9UM21-1]
    ENST00000393487; ENSP00000377127; ENSG00000071073. [Q9UM21-1]
    ENST00000409391; ENSP00000386841; ENSG00000071073. [Q9UM21-1]
    ENST00000414521; ENSP00000404889; ENSG00000071073. [Q9UM21-2]
    GeneIDi11320.
    KEGGihsa:11320.
    UCSCiuc002sze.3. human. [Q9UM21-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB000616 mRNA. Translation: BAA86388.1.
    AK304393 mRNA. Translation: BAG65228.1.
    AC064860 Genomic DNA. Translation: AAY14976.1.
    AC109826 Genomic DNA. Translation: AAY24092.1.
    CH471127 Genomic DNA. Translation: EAX01896.1.
    CH471127 Genomic DNA. Translation: EAX01897.1.
    BC127107 mRNA. Translation: AAI27108.1.
    BC127108 mRNA. Translation: AAI27109.1.
    AB053222 Genomic DNA. Translation: BAC55023.1.
    CCDSiCCDS2036.1. [Q9UM21-1]
    CCDS54380.1. [Q9UM21-2]
    RefSeqiNP_001153626.1. NM_001160154.1. [Q9UM21-2]
    NP_036346.1. NM_012214.2. [Q9UM21-1]
    UniGeneiHs.177576.

    3D structure databases

    ProteinModelPortaliQ9UM21.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi116451. 1 interaction.
    STRINGi9606.ENSP00000264968.

    Protein family/group databases

    CAZyiGT54. Glycosyltransferase Family 54.

    PTM databases

    iPTMnetiQ9UM21.
    PhosphoSiteiQ9UM21.

    Polymorphism and mutation databases

    BioMutaiMGAT4A.

    Proteomic databases

    EPDiQ9UM21.
    MaxQBiQ9UM21.
    PaxDbiQ9UM21.
    PeptideAtlasiQ9UM21.
    PRIDEiQ9UM21.

    Protocols and materials databases

    DNASUi11320.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000264968; ENSP00000264968; ENSG00000071073. [Q9UM21-1]
    ENST00000393487; ENSP00000377127; ENSG00000071073. [Q9UM21-1]
    ENST00000409391; ENSP00000386841; ENSG00000071073. [Q9UM21-1]
    ENST00000414521; ENSP00000404889; ENSG00000071073. [Q9UM21-2]
    GeneIDi11320.
    KEGGihsa:11320.
    UCSCiuc002sze.3. human. [Q9UM21-1]

    Organism-specific databases

    CTDi11320.
    GeneCardsiMGAT4A.
    HGNCiHGNC:7047. MGAT4A.
    HPAiHPA007608.
    MIMi604623. gene.
    neXtProtiNX_Q9UM21.
    PharmGKBiPA30782.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiENOG410IEDC. Eukaryota.
    ENOG410XPV0. LUCA.
    GeneTreeiENSGT00550000074578.
    HOGENOMiHOG000015085.
    HOVERGENiHBG053194.
    InParanoidiQ9UM21.
    KOiK00738.
    OMAiLQVPNIY.
    OrthoDBiEOG091G06KB.
    PhylomeDBiQ9UM21.
    TreeFamiTF324570.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BioCyciMetaCyc:HS01024-MONOMER.
    BRENDAi2.4.1.145. 2681.
    ReactomeiR-HSA-975577. N-Glycan antennae elongation.

    Miscellaneous databases

    ChiTaRSiMGAT4A. human.
    GenomeRNAii11320.
    PROiQ9UM21.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000071073.
    CleanExiHS_MGAT4A.
    ExpressionAtlasiQ9UM21. baseline and differential.
    GenevisibleiQ9UM21. HS.

    Family and domain databases

    InterProiIPR006759. Glyco_transf_54.
    [Graphical view]
    PANTHERiPTHR12062. PTHR12062. 1 hit.
    PfamiPF04666. Glyco_transf_54. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMGT4A_HUMAN
    AccessioniPrimary (citable) accession number: Q9UM21
    Secondary accession number(s): B4E2R6
    , D3DVH6, E9PEN2, Q53S97, Q86Z15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 1, 2000
    Last modified: September 7, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.