Q9UM21 (MGT4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 87.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A EC=2.4.1.145 Alternative name(s): N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa Short name=GlcNAc-T IVa Short name=GnT-IVa Short name=N-acetylglucosaminyltransferase IVa UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa Cleaved into the following chain: | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Reference proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 535 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R. |
| Cofactor | Divalent metal cations By similarity. |
| Pathway | |
| Subcellular location | Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted By similarity. |
| Tissue specificity | Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer. Ref.1 |
| Sequence similarities | Belongs to the glycosyltransferase 54 family. |
| Biophysicochemical properties | Kinetic parameters: KM=0.118 mM for UDP-GlcNAc Ref.9 |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9UM21-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9UM21-2) The sequence of this isoform differs from the canonical sequence as follows: 1-6: MRLRNG → MNYSIT 7-134: Missing. 528-535: IHIKKATN → QRVFSGWWQKSRDKAEGPQAPLLF | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 535 | 535 | Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A | PRO_0000288581 | |||||
| Chain | 93 – 535 | 443 | Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form By similarity | PRO_0000288582 | |||||
Regions | |||||||||
| Topological domain | 1 – 4 | 4 | Cytoplasmic Potential | ||||||
| Transmembrane | 5 – 27 | 23 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 28 – 535 | 508 | Lumenal Potential | ||||||
| Coiled coil | 28 – 63 | 36 | Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 77 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 458 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 6 | 6 | MRLRNG → MNYSIT in isoform 2. | VSP_046075 | |||||
| Alternative sequence | 7 – 134 | 128 | Missing in isoform 2. | VSP_046076 | |||||
| Alternative sequence | 528 – 535 | 8 | IHIKKATN → QRVFSGWWQKSRDKAEGPQA PLLF in isoform 2. | VSP_046077 | |||||
Experimental info | |||||||||
| Sequence conflict | 280 | 1 | L → F in BAG65228. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase." Yoshida A., Minowa M.T., Takamatsu S., Hara T., Oguri S., Ikenaga H., Takeuchi M. Glycobiology 9:303-310(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Liver. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Trachea. |
| [3] | "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K.Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). |
| [6] | "Alternative splicing in human N-acetylglucosaminyltransferase IVa gene." Minowa M.T., Takeuchi M. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. |
| [7] | "Unusually high expression of N-acetylglucosaminyltransferase-IVa in human choriocarcinoma cell lines: a possible enzymatic basis of the formation of abnormal biantennary sugar chain." Takamatsu S., Oguri S., Minowa M.T., Yoshida A., Nakamura K., Takeuchi M., Kobata A. Cancer Res. 59:3949-3953(1999) [PubMed] [Europe PMC] [Abstract] Cited for: OVEREXPRESSION IN CHORIOCARCINOMA. |
| [8] | "Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in pancreatic cancer." Ide Y., Miyoshi E., Nakagawa T., Gu J., Tanemura M., Nishida T., Ito T., Yamamoto H., Kozutsumi Y., Taniguchi N. Biochem. Biophys. Res. Commun. 341:478-482(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DOWN-REGULATION IN PANCREATIC CANCER. |
| [9] | "Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes." Oguri S., Yoshida A., Minowa M.T., Takeuchi M. Glycoconj. J. 23:473-480(2006) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB000616 mRNA. Translation: BAA86388.1. AK304393 mRNA. Translation: BAG65228.1. AC064860 Genomic DNA. Translation: AAY14976.1. AC109826 Genomic DNA. Translation: AAY24092.1. CH471127 Genomic DNA. Translation: EAX01896.1. CH471127 Genomic DNA. Translation: EAX01897.1. BC127107 mRNA. Translation: AAI27108.1. BC127108 mRNA. Translation: AAI27109.1. AB053222 Genomic DNA. Translation: BAC55023.1. |
| IPI | IPI00016743. IPI00908866. |
| RefSeq | NP_001153626.1. NM_001160154.1. NP_036346.1. NM_012214.2. |
| UniGene | Hs.177576. |
3D structure databases | |
| ProteinModelPortal | Q9UM21. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 9606.ENSP00000264968. |
Protein family/group databases | |
| CAZy | GT54. Glycosyltransferase Family 54. |
PTM databases | |
| PhosphoSite | Q9UM21. |
Polymorphism databases | |
| DMDM | 74721048. |
Proteomic databases | |
| PaxDb | Q9UM21. |
| PRIDE | Q9UM21. |
Protocols and materials databases | |
| DNASU | 11320. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000264968; ENSP00000264968; ENSG00000071073. ENST00000393487; ENSP00000377127; ENSG00000071073. ENST00000409391; ENSP00000386841; ENSG00000071073. ENST00000414521; ENSP00000404889; ENSG00000071073. |
| GeneID | 11320. |
| KEGG | hsa:11320. |
| UCSC | uc002sze.3. human. |
Organism-specific databases | |
| CTD | 11320. |
| GeneCards | GC02M099236. |
| HGNC | HGNC:7047. MGAT4A. |
| HPA | HPA007608. |
| MIM | 604623. gene. |
| neXtProt | NX_Q9UM21. |
| PharmGKB | PA30782. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | NOG78968. |
| HOGENOM | HOG000015085. |
| HOVERGEN | HBG053194. |
| InParanoid | Q9UM21. |
| KO | K00738. |
| OMA | DFFWAVT. |
| OrthoDB | EOG428219. |
| PhylomeDB | Q9UM21. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.145. 2681. |
| Reactome | REACT_17015. Metabolism of proteins. |
| UniPathway | UPA00378. |
Gene expression databases | |
| ArrayExpress | Q9UM21. |
| Bgee | Q9UM21. |
| CleanEx | HS_MGAT4A. |
| Genevestigator | Q9UM21. |
Family and domain databases | |
| InterPro | IPR006759. Glyco_transf_54. [Graphical view] |
| PANTHER | PTHR12062. PTHR12062. 1 hit. |
| Pfam | PF04666. Glyco_transf_54. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| GenomeRNAi | 11320. |
| NextBio | 43007. |
| SOURCE | Search... |
Entry information
| Entry name | MGT4A_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9UM21 Secondary accession number(s): B4E2R6 Q86Z15 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
