Reviewed, UniProtKB/Swiss-Prot Q9ULZ2 (STAP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Signal-transducing adaptor protein 1
      Short name=STAP-1
Alternative name(s):
    Stem cell adaptor protein 1
    BCR downstream-signaling protein 1
    Docking protein BRDG1

Gene names

Name:	STAP1
Synonyms:	BRDG1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	In BCR signaling, appears to function as a docking protein acting downstream of TEC and participates in a positive feedback loop by increasing the activity of TEC. Ref.1
Subcellular location	Cytoplasm Potential.
Post-translational modification	Phosphorylated on tyrosine by TEC. Ref.1 Ref.5
Sequence similarities	Contains 1 PH domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	SH2 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Ref.1 Traceable author statement. Source: ProtInc membrane fusion Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Signal-transducing adaptor protein 1

PRO_0000072237

Regions

Domain

25 – 121

97

PH

Domain

177 – 280

104

SH2

Amino acid modifications

Modified residue

168

1

Phosphotyrosine By similarity

Modified residue

196

1

Phosphoserine Ref.5

Secondary structure

1

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295

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

26 – 35

10

Beta strand

43 – 50

8

Beta strand

53 – 58

6

Beta strand

66 – 68

3

Beta strand

76 – 79

4

Beta strand

89 – 93

5

Beta strand

99 – 102

4

Helix

106 – 121

16

Helix

132 – 149

18

Sequences

	Sequence		Length	Mass (Da)
	Q9ULZ2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8C242AC5C61038E0	FASTA	295	34,291
	10 20 30 40 50 60 MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLIKRSGYR EYEHYWTELR GTTLFFYTDK 70 80 90 100 110 120 KSIIYVDKLD IVDLTCLTEQ NSTEKNCAKF TLVLPKEEVQ LKTENTESGE EWRGFILTVT 130 140 150 160 170 180 ELSVPQNVSL LPGQVIKLHE VLEREKKRRI ETEQSTSVEK EKEPTEDYVD VLNPMPACFY 190 200 210 220 230 240 TVSRKEATEM LQKNPSLGNM ILRPGSDSRN YSITIRQEID IPRIKHYKVM SVGQNYTIEL 250 260 270 280 290 EKPVTLPNLF SVIDYFVKET RGNLRPFICS TDENTGQEPS MEGRSEKLKK NPHIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase." Ohya K., Kajigaya S., Kitanaka A., Yoshida K., Miyazato A., Yamashita Y., Yamanaka T., Ikeda U., Shimada K., Ozawa K., Mano H. Proc. Natl. Acad. Sci. U.S.A. 96:11976-11981(1999) [PubMed: 10518561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION. Tissue: Hematopoietic.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Solution structure of the PH domain of human docking protein BRDG1." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 16-151.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB023483 mRNA. Translation: BAA85311.1.
AK313676 mRNA. Translation: BAG36427.1.
CH471057 Genomic DNA. Translation: EAX05547.1.
BC014958 mRNA. Translation: AAH14958.1.

IPI

IPI00015876.

RefSeq

NP_036240.1.

UniGene

Hs.435579

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X1F	NMR	-	A	16-151	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9ULZ2.

PTM databases

PhosphoSite

Q9ULZ2.

Proteomic databases

PeptideAtlas

Q9ULZ2.

Genome annotation databases

Ensembl

ENST00000265404; ENSP00000265404; ENSG00000035720; Homo sapiens. [Genome view]
ENST00000396225; ENSP00000379527; ENSG00000035720; Homo sapiens. [Genome view]

GeneID

26228.

KEGG

hsa:26228.

UCSC

uc003hde.2. human.

Organism-specific databases

CTD

26228.

GeneCards

GC04P068108.

H-InvDB

HIX0004249.

HGNC

HGNC:24133. STAP1.

MIM

604298. gene.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9ULZ2.

HOVERGEN

Q9ULZ2.

OMA

PMPACFY.

Enzyme and pathway databases

Pathway_Interaction_DB

kitpathway. Signaling events mediated by Stem cell factor receptor (c-Kit).

Gene expression databases

ArrayExpress

Q9ULZ2.

Bgee

Q9ULZ2.

CleanEx

HS_STAP1.

Genevestigator

Q9ULZ2.

GermOnline

ENSG00000035720. Homo sapiens.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000980. SH2.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.

Pfam

PF00169. PH. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

48387.

SOURCE

Search...

Entry information

Entry name

STAP1_HUMAN

Accession

Primary (citable) accession number: Q9ULZ2
Secondary accession number(s): B2R980

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.