Q9ULZ2 (STAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-transducing adaptor protein 1
Short name=STAP-1

Alternative name(s):
BCR downstream-signaling protein 1
Docking protein BRDG1
Stem cell adaptor protein 1

Gene names

Name:	STAP1
Synonyms:	BRDG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In BCR signaling, appears to function as a docking protein acting downstream of TEC and participates in a positive feedback loop by increasing the activity of TEC. Ref.1
Subunit structure	Interacts with KIT and CSF1R By similarity. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth-dependent manner. Ref.5
Subcellular location	Nucleus. Cytoplasm. Mitochondrion Ref.5.
Post-translational modification	Phosphorylated on tyrosine by TEC. Phosphorylated on tyrosine by KIT By similarity. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
Domain	SH2 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane fusion Traceable author statement Ref.1. Source: ProtInc intracellular protein transport Traceable author statement Ref.1. Source: ProtInc signal transduction Traceable author statement Ref.1. Source: ProtInc transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrion Inferred from direct assay Ref.5. Source: UniProtKB nucleus Inferred from direct assay Ref.5. Source: UniProtKB protein complex Inferred from direct assay PubMed 18468998. Source: MGI
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Signal-transducing adaptor protein 1

PRO_0000072237

Regions

Domain

25 – 121

97

PH

Domain

177 – 280

104

SH2

Amino acid modifications

Modified residue

168

1

Phosphotyrosine By similarity

Secondary structure

1

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295

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

26 – 35

10

Beta strand

43 – 50

8

Beta strand

53 – 58

6

Beta strand

66 – 68

3

Beta strand

76 – 79

4

Beta strand

89 – 93

5

Beta strand

99 – 102

4

Helix

106 – 121

16

Helix

132 – 149

18

Helix

184 – 193

10

Helix

195 – 197

3

Beta strand

199 – 204

6

Beta strand

208 – 217

10

Beta strand

219 – 221

3

Beta strand

223 – 232

10

Beta strand

235 – 238

4

Beta strand

240 – 242

3

Beta strand

244 – 248

5

Helix

249 – 259

11

Turn

260 – 262

3

Sequences

	Sequence		Length	Mass (Da)
	Q9ULZ2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8C242AC5C61038E0	FASTA	295	34,291
	10 20 30 40 50 60 MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLIKRSGYR EYEHYWTELR GTTLFFYTDK 70 80 90 100 110 120 KSIIYVDKLD IVDLTCLTEQ NSTEKNCAKF TLVLPKEEVQ LKTENTESGE EWRGFILTVT 130 140 150 160 170 180 ELSVPQNVSL LPGQVIKLHE VLEREKKRRI ETEQSTSVEK EKEPTEDYVD VLNPMPACFY 190 200 210 220 230 240 TVSRKEATEM LQKNPSLGNM ILRPGSDSRN YSITIRQEID IPRIKHYKVM SVGQNYTIEL 250 260 270 280 290 EKPVTLPNLF SVIDYFVKET RGNLRPFICS TDENTGQEPS MEGRSEKLKK NPHIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase." Ohya K., Kajigaya S., Kitanaka A., Yoshida K., Miyazato A., Yamashita Y., Yamanaka T., Ikeda U., Shimada K., Ozawa K., Mano H. Proc. Natl. Acad. Sci. U.S.A. 96:11976-11981(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION BY TEC. Tissue: Hematopoietic.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[5]	"S6K1-mediated disassembly of mitochondrial URI/PP1gamma complexes activates a negative feedback program that counters S6K1 survival signaling." Djouder N., Metzler S.C., Schmidt A., Wirbelauer C., Gstaiger M., Aebersold R., Hess D., Krek W. Mol. Cell 28:28-40(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH URI1, SUBCELLULAR LOCATION.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Solution structure of the PH domain of human docking protein BRDG1." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 16-151.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB023483 mRNA. Translation: BAA85311.1.
AK313676 mRNA. Translation: BAG36427.1.
CH471057 Genomic DNA. Translation: EAX05547.1.
BC014958 mRNA. Translation: AAH14958.1.

IPI

IPI00015876.

RefSeq

NP_036240.1. NM_012108.2.

UniGene

Hs.435579.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X1F	NMR	-	A	16-151	[»]
3MAZ	X-ray	1.90	A	167-285	[»]

ProteinModelPortal

Q9ULZ2.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9ULZ2. 1 interaction.

STRING

9606.ENSP00000265404.

PTM databases

PhosphoSite

Q9ULZ2.

Polymorphism databases

DMDM

62511239.

Proteomic databases

PaxDb

Q9ULZ2.

PeptideAtlas

Q9ULZ2.

PRIDE

Q9ULZ2.

Protocols and materials databases

DNASU

26228.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265404; ENSP00000265404; ENSG00000035720.
ENST00000396225; ENSP00000379527; ENSG00000035720.

GeneID

26228.

KEGG

hsa:26228.

UCSC

uc003hde.4. human.

Organism-specific databases

CTD

26228.

GeneCards

GC04P068424.

HGNC

HGNC:24133. STAP1.

HPA

HPA038529.

MIM

604298. gene.

neXtProt

NX_Q9ULZ2.

PharmGKB

PA162404948.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG47666.

HOGENOM

HOG000234375.

HOVERGEN

HBG062262.

InParanoid

Q9ULZ2.

OMA

NMILRPG.

OrthoDB

EOG4THVTM.

PhylomeDB

Q9ULZ2.

Enzyme and pathway databases

Pathway_Interaction_DB

kitpathway. Signaling events mediated by Stem cell factor receptor (c-Kit).

Gene expression databases

Bgee

Q9ULZ2.

CleanEx

HS_STAP1.

Genevestigator

Q9ULZ2.

GermOnline

ENSG00000035720. Homo sapiens.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.
3.30.505.10. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000980. SH2.
[Graphical view]

Pfam

PF00017. SH2. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9ULZ2.

GenomeRNAi

26228.

NextBio

48387.

SOURCE

Search...

Entry information

Entry name

STAP1_HUMAN

Accession

Primary (citable) accession number: Q9ULZ2
Secondary accession number(s): B2R980

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.