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Protein

A-kinase anchor protein 8-like

Gene

AKAP8L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).1 Publication6 Publications
(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters (PubMed:11884601). Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses (PubMed:11402034). In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity (PubMed:25034436).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri391 – 413C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 507C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: UniProtKB
  • DNA binding Source: InterPro
  • histone deacetylase binding Source: UniProtKB
  • lamin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • cell cycle G2/M phase transition Source: UniProtKB
  • mitotic chromosome condensation Source: UniProtKB
  • mRNA processing Source: UniProtKB
  • nuclear envelope disassembly Source: UniProtKB
  • positive regulation of histone deacetylation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of histone phosphorylation Source: UniProtKB
  • regulation of mRNA export from nucleus Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000011243-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 8-like
Short name:
AKAP8-like protein
Alternative name(s):
Helicase A-binding protein 95
Short name:
HAP95
Homologous to AKAP95 protein
Short name:
HA95
Neighbor of A-kinase-anchoring protein 95
Short name:
Neighbor of AKAP95
Gene namesi
Name:AKAP8L
Synonyms:NAKAP, NAKAP95
ORF Names:HRIHFB2018
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:29857. AKAP8L.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • cytoplasm Source: HPA
  • nuclear matrix Source: UniProtKB
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • PML body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134867364.

Polymorphism and mutation databases

BioMutaiAKAP8L.
DMDMi296439446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000753841 – 646A-kinase anchor protein 8-likeAdd BLAST646

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei208Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei208Omega-N-methylarginine; alternateCombined sources1
Modified residuei217Omega-N-methylarginineCombined sources1
Modified residuei237Omega-N-methylarginineCombined sources1
Modified residuei247Omega-N-methylarginineCombined sources1
Modified residuei257N6-acetyllysineCombined sources1
Modified residuei267PhosphothreonineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei292PhosphothreonineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei552PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9ULX6.
PaxDbiQ9ULX6.
PeptideAtlasiQ9ULX6.
PRIDEiQ9ULX6.

PTM databases

iPTMnetiQ9ULX6.
PhosphoSitePlusiQ9ULX6.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in the brain cortex (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000011243.
CleanExiHS_AKAP8L.
ExpressionAtlasiQ9ULX6. baseline and differential.
GenevisibleiQ9ULX6. HS.

Organism-specific databases

HPAiHPA042485.
HPA042546.

Interactioni

Subunit structurei

Interacts with DHX9, TMPO isoform Beta, PRPF40A, RNF43, lamin-B (PubMed:10748171, PubMed:11034899, PubMed:12538639 PubMed:16391387, PubMed:18313049). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with EBV EBNA-LP (PubMed:11160753). Interacts with HIV-1 reverse transcriptase/ribonuclease H (PubMed:25034436).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299913EBI-357530,EBI-8826747From a different organism.
HDAC3O153795EBI-357530,EBI-607682
Prpf40aQ9R1C73EBI-357530,EBI-645554From a different organism.
RNF43Q68DV72EBI-357530,EBI-1647060

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: UniProtKB
  • histone deacetylase binding Source: UniProtKB
  • lamin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117940. 37 interactors.
DIPiDIP-27541N.
IntActiQ9ULX6. 29 interactors.
MINTiMINT-1134534.
STRINGi9606.ENSP00000380557.

Structurei

3D structure databases

ProteinModelPortaliQ9ULX6.
SMRiQ9ULX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 268Sufficient for activation of CTE-mediated expression1 PublicationAdd BLAST268

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 279Nuclear localization signalSequence analysis6
Motifi280 – 296Nuclear export signal (NES)1 PublicationAdd BLAST17
Motifi362 – 364Nuclear localization signalSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 52Gly/Tyr-richAdd BLAST12
Compositional biasi589 – 597Pro-rich9
Compositional biasi602 – 608Pro-rich7

Sequence similaritiesi

Belongs to the AKAP95 family.PROSITE-ProRule annotation
Contains 2 C2H2 AKAP95-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri391 – 413C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 507C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIFX. Eukaryota.
ENOG410YCRZ. LUCA.
HOGENOMiHOG000169111.
HOVERGENiHBG104297.
InParanoidiQ9ULX6.
KOiK15978.
OrthoDBiEOG091G0836.
PhylomeDBiQ9ULX6.
TreeFamiTF105407.

Family and domain databases

InterProiIPR007071. AKAP95.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
PROSITEiPS51799. ZF_C2H2_AKAP95. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ULX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYTGFVQGS ETTLQSTYSD TSAQPTCDYG YGTWNSGTNR GYEGYGYGYG
60 70 80 90 100
YGQDNTTNYG YGMATSHSWE MPSSDTNANT SASGSASADS VLSRINQRLD
110 120 130 140 150
MVPHLETDMM QGGVYGSGGE RYDSYESCDS RAVLSERDLY RSGYDYSELD
160 170 180 190 200
PEMEMAYEGQ YDAYRDQFRM RGNDTFGPRA QGWARDARSG RPMASGYGRM
210 220 230 240 250
WEDPMGARGQ CMSGASRLPS LFSQNIIPEY GMFQGMRGGG AFPGGSRFGF
260 270 280 290 300
GFGNGMKQMR RTWKTWTTAD FRTKKKKRKQ GGSPDEPDSK ATRTDCSDNS
310 320 330 340 350
DSDNDEGTEG EATEGLEGTE AVEKGSRVDG EDEEGKEDGR EEGKEDPEKG
360 370 380 390 400
ALTTQDENGQ TKRKLQAGKK SQDKQKKRQR DRMVERIQFV CSLCKYRTFY
410 420 430 440 450
EDEMASHLDS KFHKEHFKYV GTKLPKQTAD FLQEYVTNKT KKTEELRKTV
460 470 480 490 500
EDLDGLIHQI YRDQDLTQEI AMEHFVKKVE AAHCAACDLF IPMQFGIIQK
510 520 530 540 550
HLKTMDHNRN RRLMMEQSKK SSLMVARSIL NNKLISKKLE RYLKGENPFT
560 570 580 590 600
DSPEEEKEQE EAEGGALDEG AQGEAAGISE GAEGVPAQPP VPPEPAPGAV
610 620 630 640
SPPPPPPPEE EEEGAVPLLG GALQRQIRGI PGLDVEDDEE GGGGAP
Length:646
Mass (Da):71,649
Last modified:May 18, 2010 - v3
Checksum:iEED855A81BB06585
GO
Isoform 2 (identifier: Q9ULX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-121: Missing.

Show »
Length:585
Mass (Da):65,230
Checksum:i460F073AC534A1B9
GO

Sequence cautioni

The sequence AAF86048 differs from that shown. Reason: Frameshift at position 553.Curated
The sequence BAA34791 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100D → N in AAF86048 (PubMed:10748171).Curated1
Sequence conflicti189S → N in AAF86048 (PubMed:10748171).Curated1
Sequence conflicti351 – 358ALTTQDEN → EFSWGAWC in BAA34791 (PubMed:9853615).Curated8
Sequence conflicti599 – 616AVSPP…EEGAV → GRVAATAAAPRRRRRRAPW in AAF86048 (PubMed:10748171).CuratedAdd BLAST18
Sequence conflicti641 – 646GGGGAP → EGRRGRPV in AAF86048 (PubMed:10748171).Curated6

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_068822458H → Q.Combined sources6 PublicationsCorresponds to variant rs2058322dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04442661 – 121Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025905 mRNA. Translation: BAA85003.1.
AJ243467 mRNA. Translation: CAB65092.1.
AF199414 mRNA. Translation: AAF86048.1. Frameshift.
AK295956 mRNA. Translation: BAG58736.1.
AB451326 mRNA. Translation: BAG70140.1.
AB451469 mRNA. Translation: BAG70283.1.
AC005785 Genomic DNA. No translation available.
AC006128 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84475.1.
BC000713 mRNA. Translation: AAH00713.1.
AB015332 mRNA. Translation: BAA34791.1. Different initiation.
CCDSiCCDS46005.1. [Q9ULX6-1]
CCDS77249.1. [Q9ULX6-2]
RefSeqiNP_001278407.1. NM_001291478.1.
NP_055186.3. NM_014371.3.
UniGeneiHs.399800.

Genome annotation databases

EnsembliENST00000397410; ENSP00000380557; ENSG00000011243.
ENST00000595465; ENSP00000470952; ENSG00000011243.
GeneIDi26993.
KEGGihsa:26993.
UCSCiuc002naw.2. human. [Q9ULX6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025905 mRNA. Translation: BAA85003.1.
AJ243467 mRNA. Translation: CAB65092.1.
AF199414 mRNA. Translation: AAF86048.1. Frameshift.
AK295956 mRNA. Translation: BAG58736.1.
AB451326 mRNA. Translation: BAG70140.1.
AB451469 mRNA. Translation: BAG70283.1.
AC005785 Genomic DNA. No translation available.
AC006128 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84475.1.
BC000713 mRNA. Translation: AAH00713.1.
AB015332 mRNA. Translation: BAA34791.1. Different initiation.
CCDSiCCDS46005.1. [Q9ULX6-1]
CCDS77249.1. [Q9ULX6-2]
RefSeqiNP_001278407.1. NM_001291478.1.
NP_055186.3. NM_014371.3.
UniGeneiHs.399800.

3D structure databases

ProteinModelPortaliQ9ULX6.
SMRiQ9ULX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117940. 37 interactors.
DIPiDIP-27541N.
IntActiQ9ULX6. 29 interactors.
MINTiMINT-1134534.
STRINGi9606.ENSP00000380557.

PTM databases

iPTMnetiQ9ULX6.
PhosphoSitePlusiQ9ULX6.

Polymorphism and mutation databases

BioMutaiAKAP8L.
DMDMi296439446.

Proteomic databases

EPDiQ9ULX6.
PaxDbiQ9ULX6.
PeptideAtlasiQ9ULX6.
PRIDEiQ9ULX6.

Protocols and materials databases

DNASUi26993.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397410; ENSP00000380557; ENSG00000011243.
ENST00000595465; ENSP00000470952; ENSG00000011243.
GeneIDi26993.
KEGGihsa:26993.
UCSCiuc002naw.2. human. [Q9ULX6-1]

Organism-specific databases

CTDi26993.
GeneCardsiAKAP8L.
H-InvDBHIX0014850.
HIX0115658.
HGNCiHGNC:29857. AKAP8L.
HPAiHPA042485.
HPA042546.
MIMi609475. gene.
neXtProtiNX_Q9ULX6.
PharmGKBiPA134867364.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIFX. Eukaryota.
ENOG410YCRZ. LUCA.
HOGENOMiHOG000169111.
HOVERGENiHBG104297.
InParanoidiQ9ULX6.
KOiK15978.
OrthoDBiEOG091G0836.
PhylomeDBiQ9ULX6.
TreeFamiTF105407.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000011243-MONOMER.

Miscellaneous databases

ChiTaRSiAKAP8L. human.
GeneWikiiAKAP8L.
GenomeRNAii26993.
PROiQ9ULX6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000011243.
CleanExiHS_AKAP8L.
ExpressionAtlasiQ9ULX6. baseline and differential.
GenevisibleiQ9ULX6. HS.

Family and domain databases

InterProiIPR007071. AKAP95.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
PROSITEiPS51799. ZF_C2H2_AKAP95. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKP8L_HUMAN
AccessioniPrimary (citable) accession number: Q9ULX6
Secondary accession number(s): B4DJ74
, B5BU90, O94792, Q96J58, Q9NRQ0, Q9UGM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.