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Protein

Targeting protein for Xklp2

Gene

TPX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).3 Publications

GO - Molecular functioni

  • ATP binding Source: ProtInc
  • GTP binding Source: ProtInc
  • importin-alpha family protein binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • activation of protein kinase activity Source: UniProtKB
  • apoptotic process Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell proliferation Source: ProtInc
  • mitotic cell cycle Source: ProtInc
  • mitotic spindle assembly Source: UniProtKB
  • regulation of G2/M transition of mitotic cell cycle Source: Reactome
  • regulation of mitotic spindle organization Source: UniProtKB
  • regulation of signal transduction by p53 class mediator Source: Reactome

Keywordsi

Biological processApoptosis, Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Targeting protein for Xklp2
Alternative name(s):
Differentially expressed in cancerous and non-cancerous lung cells 2
Short name:
DIL-2
Hepatocellular carcinoma-associated antigen 519
Hepatocellular carcinoma-associated antigen 90
Protein fls353
Restricted expression proliferation-associated protein 100
Short name:
p100
Gene namesi
Name:TPX2
Synonyms:C20orf1, C20orf2, DIL2, HCA519
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000088325.15
HGNCiHGNC:1249 TPX2
MIMi605917 gene
neXtProtiNX_Q9ULW0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi22974
OpenTargetsiENSG00000088325
PharmGKBiPA25638

Chemistry databases

ChEMBLiCHEMBL5389

Polymorphism and mutation databases

BioMutaiTPX2
DMDMi13124096

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000655811 – 747Targeting protein for Xklp2Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphothreonineCombined sources1
Modified residuei72PhosphothreonineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei125PhosphoserineCombined sources1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei147PhosphothreonineCombined sources1
Modified residuei257PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei293PhosphoserineCombined sources1
Modified residuei305N6-acetyllysineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei338PhosphothreonineCombined sources1
Modified residuei359PhosphoserineCombined sources1
Modified residuei369PhosphothreonineCombined sources1
Modified residuei375N6-acetyllysineBy similarity1
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei486PhosphoserineCombined sources1
Modified residuei499PhosphothreonineCombined sources1
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki641Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei738PhosphoserineCombined sources1
Cross-linki740Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9ULW0
PaxDbiQ9ULW0
PeptideAtlasiQ9ULW0
PRIDEiQ9ULW0

PTM databases

iPTMnetiQ9ULW0
PhosphoSitePlusiQ9ULW0

Expressioni

Tissue specificityi

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Developmental stagei

Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.

Gene expression databases

BgeeiENSG00000088325
CleanExiHS_TPX2
ExpressionAtlasiQ9ULW0 baseline and differential
GenevisibleiQ9ULW0 HS

Organism-specific databases

HPAiHPA005487

Interactioni

Subunit structurei

Interacts with AURKA (PubMed:14580337, PubMed:18662907, PubMed:18663142). Interacts with importin-alpha; leading to inactivate TPX2 (PubMed:26165940). Interacts with HNRNPU; this interaction recruits HNRNPU to spindle microtubules (MTs) (PubMed:21242313, PubMed:25986610).6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • importin-alpha family protein binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116624, 41 interactors
CORUMiQ9ULW0
DIPiDIP-36727N
DIP-46212N
IntActiQ9ULW0, 22 interactors
MINTiQ9ULW0
STRINGi9606.ENSP00000300403

Structurei

Secondary structure

1747
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni19 – 21Combined sources3
Helixi30 – 32Combined sources3
Helixi33 – 41Combined sources9
Helixi327 – 336Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OL5X-ray2.50B1-43[»]
3E5AX-ray2.30B1-43[»]
3HA6X-ray2.36B1-43[»]
4C3PX-ray2.69B/E1-43[»]
5LXMX-ray2.08D6-43[»]
6BJCelectron microscopy3.30P/T1-747[»]
ProteinModelPortaliQ9ULW0
SMRiQ9ULW0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ULW0

Family & Domainsi

Sequence similaritiesi

Belongs to the TPX2 family.Curated

Phylogenomic databases

eggNOGiENOG410IIEY Eukaryota
ENOG4110SQ6 LUCA
GeneTreeiENSGT00390000009842
HOGENOMiHOG000231739
HOVERGENiHBG057334
InParanoidiQ9ULW0
KOiK16812
OMAiSRPCPTK
OrthoDBiEOG091G0MIV
PhylomeDBiQ9ULW0
TreeFamiTF328997

Family and domain databases

InterProiView protein in InterPro
IPR015128 Aurora-A-bd
IPR027329 TPX2_C
IPR027330 TPX2_central_dom
IPR009675 TPX2_fam
PANTHERiPTHR14326 PTHR14326, 1 hit
PfamiView protein in Pfam
PF09041 Aurora-A_bind, 1 hit
PF06886 TPX2, 1 hit
PF12214 TPX2_importin, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ULW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQVKSSYSY DAPSDFINFS SLDDEGDTQN IDSWFEEKAN LENKLLGKNG
60 70 80 90 100
TGGLFQGKTP LRKANLQQAI VTPLKPVDNT YYKEAEKENL VEQSIPSNAC
110 120 130 140 150
SSLEVEAAIS RKTPAQPQRR SLRLSAQKDL EQKEKHHVKM KAKRCATPVI
160 170 180 190 200
IDEILPSKKM KVSNNKKKPE EEGSAHQDTA EKNASSPEKA KGRHTVPCMP
210 220 230 240 250
PAKQKFLKST EEQELEKSMK MQQEVVEMRK KNEEFKKLAL AGIGQPVKKS
260 270 280 290 300
VSQVTKSVDF HFRTDERIKQ HPKNQEEYKE VNFTSELRKH PSSPARVTKG
310 320 330 340 350
CTIVKPFNLS QGKKRTFDET VSTYVPLAQQ VEDFHKRTPN RYHLRSKKDD
360 370 380 390 400
INLLPSKSSV TKICRDPQTP VLQTKHRARA VTCKSTAELE AEELEKLQQY
410 420 430 440 450
KFKARELDPR ILEGGPILPK KPPVKPPTEP IGFDLEIEKR IQERESKKKT
460 470 480 490 500
EDEHFEFHSR PCPTKILEDV VGVPEKKVLP ITVPKSPAFA LKNRIRMPTK
510 520 530 540 550
EDEEEDEPVV IKAQPVPHYG VPFKPQIPEA RTVEICPFSF DSRDKERQLQ
560 570 580 590 600
KEKKIKELQK GEVPKFKALP LPHFDTINLP EKKVKNVTQI EPFCLETDRR
610 620 630 640 650
GALKAQTWKH QLEEELRQQK EAACFKARPN TVISQEPFVP KKEKKSVAEG
660 670 680 690 700
LSGSLVQEPF QLATEKRAKE RQELEKRMAE VEAQKAQQLE EARLQEEEQK
710 720 730 740
KEELARLRRE LVHKANPIRK YQGLEIKSSD QPLTVPVSPK FSTRFHC
Length:747
Mass (Da):85,653
Last modified:February 21, 2001 - v2
Checksum:iE028E0BB50BBCA0F
GO
Isoform 2 (identifier: Q9ULW0-2) [UniParc]FASTAAdd to basket
Also known as: HCA90

The sequence of this isoform differs from the canonical sequence as follows:
     351-351: I → IKTGSCSVTQAGVQWRDHGSLQCPTPGLKQSSCLSLP

Note: No experimental confirmation available.
Show »
Length:783
Mass (Da):89,393
Checksum:i10C8E4122A53BF9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182K → N in BAA76931 (Ref. 3) Curated1
Sequence conflicti273K → E in BAA85893 (PubMed:10512675).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036269464T → N in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057355351I → IKTGSCSVTQAGVQWRDHGS LQCPTPGLKQSSCLSLP in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027467 mRNA Translation: BAA85893.1
AF098158 mRNA Translation: AAF03248.1
AB024704 mRNA Translation: BAA76931.1
AF146731 mRNA Translation: AAD33965.1
AF287265 mRNA Translation: AAK83033.1
AL160175 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76418.1
CH471077 Genomic DNA Translation: EAW76422.1
BC004136 mRNA Translation: AAH04136.1
BC020207 mRNA Translation: AAH20207.1
AL117534 mRNA Translation: CAB55982.1
AF244547 mRNA Translation: AAF81695.1
CCDSiCCDS13190.1 [Q9ULW0-1]
PIRiT17292
RefSeqiNP_036244.2, NM_012112.4 [Q9ULW0-1]
XP_011526999.1, XM_011528697.2 [Q9ULW0-1]
XP_011527001.1, XM_011528699.2 [Q9ULW0-1]
UniGeneiHs.244580

Genome annotation databases

EnsembliENST00000300403; ENSP00000300403; ENSG00000088325 [Q9ULW0-1]
ENST00000340513; ENSP00000341145; ENSG00000088325 [Q9ULW0-2]
GeneIDi22974
KEGGihsa:22974
UCSCiuc002wwp.2 human [Q9ULW0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTPX2_HUMAN
AccessioniPrimary (citable) accession number: Q9ULW0
Secondary accession number(s): Q96RR5
, Q9H1R4, Q9NRA3, Q9UFN9, Q9UL00, Q9Y2M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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