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Protein

Pre-mRNA-splicing factor ISY1 homolog

Gene

ISY1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in pre-mRNA splicing as component of the spliceosome.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ISY1 homolog
Gene namesi
Name:ISY1
Synonyms:KIAA1160
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000240682.9
HGNCiHGNC:29201 ISY1
MIMi612764 gene
neXtProtiNX_Q9ULR0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000240682
ENSG00000261796
PharmGKBiPA162392343

Polymorphism and mutation databases

BioMutaiISY1
DMDMi300669687

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002358131 – 285Pre-mRNA-splicing factor ISY1 homologAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei127N6-acetyllysineCombined sources1
Modified residuei247PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ULR0
MaxQBiQ9ULR0
PaxDbiQ9ULR0
PeptideAtlasiQ9ULR0
PRIDEiQ9ULR0

PTM databases

iPTMnetiQ9ULR0
PhosphoSitePlusiQ9ULR0

Expressioni

Gene expression databases

BgeeiENSG00000240682
CleanExiHS_ISY1
ExpressionAtlasiQ9ULR0 baseline and differential
GenevisibleiQ9ULR0 HS

Organism-specific databases

HPAiHPA006312
HPA016995

Interactioni

Subunit structurei

Identified in the spliceosome C complex (PubMed:11991638). Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE (PubMed:17981804). Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex (PubMed:25599396). The IB complex does not contain PRPF19 (PubMed:25599396).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121531, 76 interactors
1529628, 19 interactors
CORUMiQ9ULR0
DIPiDIP-56636N
IntActiQ9ULR0, 14 interactors
MINTiQ9ULR0
STRINGi9606.ENSP00000411822

Structurei

3D structure databases

ProteinModelPortaliQ9ULR0
SMRiQ9ULR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi201 – 211Poly-GluAdd BLAST11

Sequence similaritiesi

Belongs to the ISY1 family.Curated

Phylogenomic databases

eggNOGiKOG3068 Eukaryota
ENOG410XPPC LUCA
GeneTreeiENSGT00390000014109
HOGENOMiHOG000173970
HOVERGENiHBG061071
InParanoidiQ9ULR0
KOiK12870
OMAiWEVQIRN
OrthoDBiEOG091G0JHQ
PhylomeDBiQ9ULR0
TreeFamiTF105841

Family and domain databases

Gene3Di1.10.287.660, 1 hit
InterProiView protein in InterPro
IPR029012 Helix_hairpin_bin_sf
IPR009360 Isy1
IPR037200 Isy1_sf
PANTHERiPTHR13021 PTHR13021, 1 hit
PfamiView protein in Pfam
PF06246 Isy1, 1 hit
SUPFAMiSSF140102 SSF140102, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q9ULR0-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII
60 70 80 90 100
GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEVR IKELGGPDYG
110 120 130 140 150
KVGPKMLDHE GKEVPGNRGY KYFGAAKDLP GVRELFEKEP LPPPRKTRAE
160 170 180 190 200
LMKAIDFEYY GYLDEDDGVI VPLEQEYEKK LRAELVEKWK AEREARLARG
210 220 230 240 250
EKEEEEEEEE EINIYAVTEE ESDEEGSQEK GGDDSQQKFI AHVPVPSQQE
260 270 280
IEEALVRRKK MELLQKYASE TLQAQSEEAR RLLGY
Length:285
Mass (Da):32,992
Last modified:July 13, 2010 - v3
Checksum:iAB43602EBCB51B2C
GO
Isoform 2 (identifier: Q9ULR0-1) [UniParc]FASTAAdd to basket
Also known as: ISY1-RAB43

The sequence of this isoform differs from the canonical sequence as follows:
     285-285: Y → CRSGTRPARSGSAPSPRATTAVPMGPSLPTTSPRGAPSCRCLTGLRM

Note: Based on a readthrough transcript which may produce a ISY1-RAB43 fusion protein. No experimental confirmation available.
Show »
Length:331
Mass (Da):37,567
Checksum:iD262B3ED1DFC6BF4
GO
Isoform 1 (identifier: Q9ULR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-140: P → RQVRWLMPVIPALWEAEAGGSQA

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):35,443
Checksum:i962FF7178F72DD55
GO

Sequence cautioni

The sequence BAA86474 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039410140P → RQVRWLMPVIPALWEAEAGG SQA in isoform 1. 1 Publication1
Alternative sequenceiVSP_039411285Y → CRSGTRPARSGSAPSPRATT AVPMGPSLPTTSPRGAPSCR CLTGLRM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032986 mRNA Translation: BAA86474.1 Different initiation.
BC004442 mRNA Translation: AAH04442.1
BC007409 mRNA Translation: AAH07409.1
BC019849 mRNA Translation: AAH19849.1
CCDSiCCDS43149.1 [Q9ULR0-3]
CCDS56277.1 [Q9ULR0-2]
RefSeqiNP_001186398.1, NM_001199469.1 [Q9ULR0-2]
NP_001191819.1, NM_001204890.1 [Q9ULR0-1]
NP_065752.1, NM_020701.3 [Q9ULR0-3]
UniGeneiHs.512661

Genome annotation databases

EnsembliENST00000273541; ENSP00000273541; ENSG00000240682 [Q9ULR0-2]
ENST00000393295; ENSP00000376973; ENSG00000240682 [Q9ULR0-3]
GeneIDi100534599
57461
KEGGihsa:100534599
hsa:57461
UCSCiuc003elp.3 human [Q9ULR0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiISY1_HUMAN
AccessioniPrimary (citable) accession number: Q9ULR0
Secondary accession number(s): Q96IL2, Q9BT05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 13, 2010
Last modified: March 28, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health