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Protein

Pre-mRNA-splicing factor ISY1 homolog

Gene

ISY1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in pre-mRNA splicing.Curated

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ISY1 homolog
Gene namesi
Name:ISY1
Synonyms:KIAA1160
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29201. ISY1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000240682.
ENSG00000261796.
PharmGKBiPA162392343.

Polymorphism and mutation databases

BioMutaiISY1.
DMDMi300669687.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002358131 – 285Pre-mRNA-splicing factor ISY1 homologAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei127N6-acetyllysineCombined sources1
Modified residuei247PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ULR0.
MaxQBiQ9ULR0.
PaxDbiQ9ULR0.
PeptideAtlasiQ9ULR0.
PRIDEiQ9ULR0.

PTM databases

iPTMnetiQ9ULR0.
PhosphoSitePlusiQ9ULR0.

Expressioni

Gene expression databases

BgeeiENSG00000240682.
CleanExiHS_ISY1.
ExpressionAtlasiQ9ULR0. baseline and differential.
GenevisibleiQ9ULR0. HS.

Organism-specific databases

HPAiHPA006312.
HPA016995.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DHX8Q145622EBI-2557660,EBI-2511477
XAB2Q9HCS76EBI-2557660,EBI-295232

Protein-protein interaction databases

BioGridi121531. 56 interactors.
1529628. 19 interactors.
DIPiDIP-56636N.
IntActiQ9ULR0. 10 interactors.
MINTiMINT-3082306.
STRINGi9606.ENSP00000411822.

Structurei

3D structure databases

ProteinModelPortaliQ9ULR0.
SMRiQ9ULR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi201 – 211Poly-GluAdd BLAST11

Sequence similaritiesi

Belongs to the ISY1 family.Curated

Phylogenomic databases

eggNOGiKOG3068. Eukaryota.
ENOG410XPPC. LUCA.
GeneTreeiENSGT00390000014109.
HOGENOMiHOG000173970.
HOVERGENiHBG061071.
InParanoidiQ9ULR0.
KOiK12870.
OMAiRDKMARD.
OrthoDBiEOG091G0JHQ.
PhylomeDBiQ9ULR0.
TreeFamiTF105841.

Family and domain databases

InterProiIPR009360. Isy1.
[Graphical view]
PANTHERiPTHR13021. PTHR13021. 1 hit.
PfamiPF06246. Isy1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q9ULR0-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII
60 70 80 90 100
GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEVR IKELGGPDYG
110 120 130 140 150
KVGPKMLDHE GKEVPGNRGY KYFGAAKDLP GVRELFEKEP LPPPRKTRAE
160 170 180 190 200
LMKAIDFEYY GYLDEDDGVI VPLEQEYEKK LRAELVEKWK AEREARLARG
210 220 230 240 250
EKEEEEEEEE EINIYAVTEE ESDEEGSQEK GGDDSQQKFI AHVPVPSQQE
260 270 280
IEEALVRRKK MELLQKYASE TLQAQSEEAR RLLGY
Length:285
Mass (Da):32,992
Last modified:July 13, 2010 - v3
Checksum:iAB43602EBCB51B2C
GO
Isoform 2 (identifier: Q9ULR0-1) [UniParc]FASTAAdd to basket
Also known as: ISY1-RAB43

The sequence of this isoform differs from the canonical sequence as follows:
     285-285: Y → CRSGTRPARSGSAPSPRATTAVPMGPSLPTTSPRGAPSCRCLTGLRM

Note: Based on a readthrough transcript which may produce a ISY1-RAB43 fusion protein. No experimental confirmation available.
Show »
Length:331
Mass (Da):37,567
Checksum:iD262B3ED1DFC6BF4
GO
Isoform 1 (identifier: Q9ULR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-140: P → RQVRWLMPVIPALWEAEAGGSQA

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):35,443
Checksum:i962FF7178F72DD55
GO

Sequence cautioni

The sequence BAA86474 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039410140P → RQVRWLMPVIPALWEAEAGG SQA in isoform 1. 1 Publication1
Alternative sequenceiVSP_039411285Y → CRSGTRPARSGSAPSPRATT AVPMGPSLPTTSPRGAPSCR CLTGLRM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032986 mRNA. Translation: BAA86474.1. Different initiation.
BC004442 mRNA. Translation: AAH04442.1.
BC007409 mRNA. Translation: AAH07409.1.
BC019849 mRNA. Translation: AAH19849.1.
CCDSiCCDS43149.1. [Q9ULR0-3]
CCDS56277.1. [Q9ULR0-2]
RefSeqiNP_001186398.1. NM_001199469.1. [Q9ULR0-2]
NP_001191819.1. NM_001204890.1. [Q9ULR0-1]
NP_065752.1. NM_020701.3. [Q9ULR0-3]
UniGeneiHs.512661.

Genome annotation databases

EnsembliENST00000273541; ENSP00000273541; ENSG00000240682. [Q9ULR0-2]
ENST00000393295; ENSP00000376973; ENSG00000240682. [Q9ULR0-3]
GeneIDi100534599.
57461.
KEGGihsa:100534599.
hsa:57461.
UCSCiuc003elp.3. human. [Q9ULR0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032986 mRNA. Translation: BAA86474.1. Different initiation.
BC004442 mRNA. Translation: AAH04442.1.
BC007409 mRNA. Translation: AAH07409.1.
BC019849 mRNA. Translation: AAH19849.1.
CCDSiCCDS43149.1. [Q9ULR0-3]
CCDS56277.1. [Q9ULR0-2]
RefSeqiNP_001186398.1. NM_001199469.1. [Q9ULR0-2]
NP_001191819.1. NM_001204890.1. [Q9ULR0-1]
NP_065752.1. NM_020701.3. [Q9ULR0-3]
UniGeneiHs.512661.

3D structure databases

ProteinModelPortaliQ9ULR0.
SMRiQ9ULR0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121531. 56 interactors.
1529628. 19 interactors.
DIPiDIP-56636N.
IntActiQ9ULR0. 10 interactors.
MINTiMINT-3082306.
STRINGi9606.ENSP00000411822.

PTM databases

iPTMnetiQ9ULR0.
PhosphoSitePlusiQ9ULR0.

Polymorphism and mutation databases

BioMutaiISY1.
DMDMi300669687.

Proteomic databases

EPDiQ9ULR0.
MaxQBiQ9ULR0.
PaxDbiQ9ULR0.
PeptideAtlasiQ9ULR0.
PRIDEiQ9ULR0.

Protocols and materials databases

DNASUi57461.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273541; ENSP00000273541; ENSG00000240682. [Q9ULR0-2]
ENST00000393295; ENSP00000376973; ENSG00000240682. [Q9ULR0-3]
GeneIDi100534599.
57461.
KEGGihsa:100534599.
hsa:57461.
UCSCiuc003elp.3. human. [Q9ULR0-3]

Organism-specific databases

CTDi100534599.
57461.
GeneCardsiISY1.
HGNCiHGNC:29201. ISY1.
HPAiHPA006312.
HPA016995.
MIMi612764. gene.
neXtProtiNX_Q9ULR0.
OpenTargetsiENSG00000240682.
ENSG00000261796.
PharmGKBiPA162392343.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3068. Eukaryota.
ENOG410XPPC. LUCA.
GeneTreeiENSGT00390000014109.
HOGENOMiHOG000173970.
HOVERGENiHBG061071.
InParanoidiQ9ULR0.
KOiK12870.
OMAiRDKMARD.
OrthoDBiEOG091G0JHQ.
PhylomeDBiQ9ULR0.
TreeFamiTF105841.

Enzyme and pathway databases

ReactomeiR-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ9ULR0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000240682.
CleanExiHS_ISY1.
ExpressionAtlasiQ9ULR0. baseline and differential.
GenevisibleiQ9ULR0. HS.

Family and domain databases

InterProiIPR009360. Isy1.
[Graphical view]
PANTHERiPTHR13021. PTHR13021. 1 hit.
PfamiPF06246. Isy1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISY1_HUMAN
AccessioniPrimary (citable) accession number: Q9ULR0
Secondary accession number(s): Q96IL2, Q9BT05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 13, 2010
Last modified: November 30, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.