Q9ULM6 (CNOT6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCR4-NOT transcription complex subunit 6
EC=3.1.-.-

Alternative name(s):
CCR4 carbon catabolite repression 4-like
Carbon catabolite repressor protein 4 homolog
Cytoplasmic deadenylase

Gene names

Name:	CNOT6
Synonyms:	CCR4, KIAA1194

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Poly(A) nuclease involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Has 3'-5' RNase activity. The CCR4-NOT complex functions as general transcription regulation complex By similarity. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA. The increase of ligand-dependent ESR1-mediated transcription is much smaller, if any. Ref.4 Ref.5 Ref.7
Cofactor	Magnesium.
Subunit structure	Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Interacts with UNR. Interacts with ZNF335. Ref.4 Ref.6 Ref.7
Subcellular location	Cytoplasm Probable. Nucleus Probable.
Sequence similarities	Belongs to the CCR4/nocturin family. Contains 4 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Leucine-rich repeat Repeat
Ligand	Magnesium Metal-binding RNA-binding
Molecular function	Activator Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay Inferred from direct assay PubMed 20065043. Source: UniProtKB gene silencing by miRNA Traceable author statement PubMed 20065043. Source: UniProtKB nuclear-transcribed mRNA poly(A) tail shortening Traceable author statement. Source: Reactome positive regulation of ligand-dependent nuclear receptor transcription coactivator activity Inferred from direct assay Ref.7. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	CCR4-NOT complex Inferred from direct assay PubMed 19558367. Source: UniProtKB cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW poly(A)-specific ribonuclease activity Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	CCR4-NOT transcription complex subunit 6		PRO_0000218573
Regions
	Repeat	52 – 73	22	LRR 1
	Repeat	75 – 96	22	LRR 2
	Repeat	98 – 120	23	LRR 3
	Repeat	121 – 143	23	LRR 4
Sites
	Metal binding	240	1	Magnesium By similarity
Amino acid modifications
	Modified residue	146	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ULM6 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: DA9FF7A059BFB583	FASTA	557	63,307
	10 20 30 40 50 60 MPKEKYEPPD PRRMYTIMSS EEAANGKKSH WAELEISGKV RSLSASLWSL THLTALHLSD 70 80 90 100 110 120 NSLSRIPSDI AKLHNLVYLD LSSNKIRSLP AELGNMVSLR ELHLNNNLLR VLPFELGKLF 130 140 150 160 170 180 QLQTLGLKGN PLTQDILNLY QEPDGTRRLL NYLLDNLSGT AKRITTEQPP PRSWIMLQEP 190 200 210 220 230 240 DRTRPTALFS VMCYNVLCDK YATRQLYGYC PSWALNWDYR KKAIIQEILS CNADIVSLQE 250 260 270 280 290 300 VETEQYYSFF LVELKERGYN GFFSPKSRAR TMSEQERKHV DGCAIFFKTE KFTLVQKHTV 310 320 330 340 350 360 EFNQLAMANS EGSEAMLNRV MTKDNIGVAV LLELRKESIE MPSGKPHLGT EKQLILVANA 370 380 390 400 410 420 HMHWDPEYSD VKLVQTMMFL SEVKNIIDKA SRNLKSSVLG EFGTIPLVLC ADLNSLPDSG 430 440 450 460 470 480 VVEYLSTGGV ETNHKDFKEL RYNESLTNFS CHGKNGTTNG RITHGFKLQS AYESGLMPYT 490 500 510 520 530 540 NYTFDFKGII DYIFYSKPQL NTLGILGPLD HHWLVENNIS GCPHPLIPSD HFSLFAQLEL 550 LLPFLPQVNG IHLPGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding." Dupressoir A., Morel A.-P., Barbot W., Loireau M.-P., Corbo L., Heidmann T. BMC Genomics 2:9-9(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CNOT8; CHAF1A AND CHAF1B.
[5]	"CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase." Chen J., Chiang Y.-C., Denis C.L. EMBO J. 21:1414-1426(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"UNR, a new partner of poly(A)-binding protein, plays a key role in translationally coupled mRNA turnover mediated by the c-fos major coding-region determinant." Chang T.-C., Yamashita A., Chen C.-Y.A., Yamashita Y., Zhu W., Durdan S., Kahvejian A., Sonenberg N., Shyu A.-B. Genes Dev. 18:2010-2023(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UNR.
[7]	"Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1." Garapaty S., Mahajan M.A., Samuels H.H. J. Biol. Chem. 283:6806-6816(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZNF335.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB033020 mRNA. Translation: BAA86508.1. CH471165 Genomic DNA. Translation: EAW53752.1. CH471165 Genomic DNA. Translation: EAW53753.1. BC152469 mRNA. Translation: AAI52470.1.
IPI	IPI00008205.
RefSeq	NP_056270.2. NM_015455.3.
UniGene	Hs.608380.
3D structure databases
ProteinModelPortal	Q9ULM6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46838N.
IntAct	Q9ULM6. 10 interactions.
STRING	9606.ENSP00000261951.
PTM databases
PhosphoSite	Q9ULM6.
Polymorphism databases
DMDM	46396033.
Proteomic databases
PaxDb	Q9ULM6.
PRIDE	Q9ULM6.
Protocols and materials databases
DNASU	57472.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261951; ENSP00000261951; ENSG00000113300. ENST00000393356; ENSP00000377024; ENSG00000113300.
GeneID	57472.
KEGG	hsa:57472.
UCSC	uc003mlx.3. human.
Organism-specific databases
CTD	57472.
GeneCards	GC05P179921.
HGNC	HGNC:14099. CNOT6.
HPA	HPA044568.
MIM	608951. gene.
neXtProt	NX_Q9ULM6.
PharmGKB	PA26677.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4886.
HOGENOM	HOG000294222.
HOVERGEN	HBG052641.
InParanoid	Q9ULM6.
KO	K12603.
OMA	YATSDTY.
OrthoDB	EOG43TZV7.
PhylomeDB	Q9ULM6.
Enzyme and pathway databases
Reactome	REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9ULM6.
Bgee	Q9ULM6.
CleanEx	HS_CCR4. HS_CNOT6.
Genevestigator	Q9ULM6.
GermOnline	ENSG00000113300. Homo sapiens.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00369. LRR_TYP. 2 hits. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57472.
NextBio	63703.
SOURCE	Search...

Entry information

Entry name

CNOT6_HUMAN

Accession

Primary (citable) accession number: Q9ULM6
Secondary accession number(s): A7MD46, D3DWR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.