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Q9ULJ3 (ZBT21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 21
Alternative name(s):
Zinc finger protein 295
Gene names
Name:ZBTB21
Synonyms:KIAA1227, ZNF295
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1066 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcription repressor. Ref.1

Subunit structure

Homodimer. Interacts with ZBTB14. Ref.1

Subcellular location

Nucleus. Note: Colocalizes with ZBTB14 in nucleus in HEK293 cells. Ref.1

Tissue specificity

Ubiquitous in fetal and adult tissues. Ref.1

Sequence similarities

Contains 1 BTB (POZ) domain.

Contains 8 C2H2-type zinc fingers.

Sequence caution

The sequence BAA86541.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ULJ3-1)

Also known as: ZNF295L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ULJ3-2)

Also known as: ZNF295S;

The sequence of this isoform differs from the canonical sequence as follows:
     535-735: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10661066Zinc finger and BTB domain-containing protein 21
PRO_0000047515

Regions

Domain30 – 9667BTB
Zinc finger546 – 56924C2H2-type 1
Zinc finger575 – 59824C2H2-type 2
Zinc finger670 – 69223C2H2-type 3
Zinc finger748 – 77023C2H2-type 4; atypical
Zinc finger775 – 79824C2H2-type 5
Zinc finger909 – 93224C2H2-type 6; atypical
Zinc finger937 – 95923C2H2-type 7
Zinc finger1043 – 106523C2H2-type 8
Region30 – 9667Mediates homodimerization

Amino acid modifications

Modified residue3811Phosphoserine Ref.7
Modified residue4111Phosphoserine Ref.8 Ref.10
Modified residue4221Phosphoserine Ref.7 Ref.8
Modified residue4311Phosphothreonine Ref.8 Ref.10 Ref.12
Modified residue4341Phosphoserine Ref.8 Ref.12
Modified residue4351Phosphoserine Ref.8 Ref.10
Modified residue4381Phosphoserine Ref.8
Modified residue6051Phosphoserine Ref.8 Ref.11
Modified residue7141Phosphoserine Ref.6 Ref.11
Modified residue10031Phosphoserine Ref.8 Ref.10

Natural variations

Alternative sequence535 – 735201Missing in isoform 2.
VSP_041349
Natural variant1851N → S.
Corresponds to variant rs871545 [ dbSNP | Ensembl ].
VAR_052807
Natural variant2181K → Q.
Corresponds to variant rs871546 [ dbSNP | Ensembl ].
VAR_052808

Experimental info

Sequence conflict9611S → P in AAH63290. Ref.5

Secondary structure

................ 1066
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ZNF295L) [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: E1779E26943FDAC8

FASTA1,066118,870
        10         20         30         40         50         60 
MEGLLHYINP AHAISLLSAL NEERLKGQLC DVLLIVGDQK FRAHKNVLAA SSEYFQSLFT 

        70         80         90        100        110        120 
NKENESQTVF QLDFCEPDAF DNVLNYIYSS SLFVEKSSLA AVQELGYSLG ISFLTNIVSK 

       130        140        150        160        170        180 
TPQAPFPTCP NRKKVFVEDD ENSSQKRSVI VCQSRNEAQG KTVSQNQPDV SHTSRPSPSI 

       190        200        210        220        230        240 
AVKANTNKPH VPKPIEPLHN LSLTEKSWPK DSSVVYAKSL EHSGSLDDPN RISLVKRNAV 

       250        260        270        280        290        300 
LPSKPLQDRE AMDDKPGVSG QLPKGKALEL ALKRPRPPVL SVCSSSETPY LLKETNKGNG 

       310        320        330        340        350        360 
QGEDRNLLYY SKLGLVIPSS GSGSGNQSID RSGPLVKSLL RRSLSMDSQV PVYSPSIDLK 

       370        380        390        400        410        420 
SSQGSSSVSS DAPGNVLCAL SQKSSLKDCS EKTALDDRPQ VLQPHRLRSF SASQSTDREG 

       430        440        450        460        470        480 
ASPVTEVRIK TEPSSPLSDP SDIIRVTVGD AATTAAASSS SVTRDLSLKT EDDQKDMSRL 

       490        500        510        520        530        540 
PAKRRFQADR RLPFKKLKVN EHGSPVSEDN FEEGSSPTLL DADFPDSDLN KDEFGELEGT 

       550        560        570        580        590        600 
RPNKKFKCKH CLKIFRSTAG LHRHVNMYHN PEKPYACDIC HKRFHTNFKV WTHCQTQHGI 

       610        620        630        640        650        660 
VKNPSPASSS HAVLDEKFQR KLIDIVRERE IKKALIIKLR RGKPGFQGQS SSQAQQVIKR 

       670        680        690        700        710        720 
NLRSRAKGAY ICTYCGKAYR FLSQFKQHIK MHPGEKPLGV NKVAKPKEHA PLASPVENKE 

       730        740        750        760        770        780 
VYQCRLCNAK LSSLLEQGSH ERLCRNAAVC PYCSLRFFSP ELKQEHESKC EYKKLTCLEC 

       790        800        810        820        830        840 
MRTFKSSFSI WRHQVEVHNQ NNMAPTENFS LPVLDHNGDV TGSSRPQSQP EPNKVNHIVT 

       850        860        870        880        890        900 
TKDDNVFSDS SEQVNFDSED SSCLPEDLSL SKQLKIQVKE EPVEEAEEEA PEASTAPKEA 

       910        920        930        940        950        960 
GPSKEASLWP CEKCGKMFTV HKQLERHQEL LCSVKPFICH VCNKAFRTNF RLWSHFQSHM 

       970        980        990       1000       1010       1020 
SQASEESAHK ESEVCPVPTN SPSPPPLPPP PPLPKIQPLE PDSPTGLSEN PTPATEKLFV 

      1030       1040       1050       1060 
PQESDTLFYH APPLSAITFK RQFMCKLCHR TFKTAFSLWS HEQTHN 

« Hide

Isoform 2 (ZNF295S) [UniParc].

Checksum: 722AB5BE41C99614
Show »

FASTA86595,807

References

« Hide 'large scale' references
[1]"Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161."
Wang J., Kudoh J., Takayanagi A., Shimizu N.
Biochem. Biophys. Res. Commun. 327:615-627(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH ZBTB14, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Fetal kidney and Testis.
[2]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-714, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-422, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411; SER-422; THR-431; SER-434; SER-435; SER-438; SER-605 AND SER-1003, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411; THR-431; SER-435 AND SER-1003, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605 AND SER-714, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-431 AND SER-434, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Solution structure of ZF-C2H2 domains from human zinc finger protein 295."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 713-806.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB041014 mRNA. Translation: BAD74063.1.
AB041015 mRNA. Translation: BAD74064.1.
AB033053 mRNA. Translation: BAA86541.1. Different initiation.
AP001745 Genomic DNA. Translation: BAA95529.1.
CH471079 Genomic DNA. Translation: EAX09579.1.
CH471079 Genomic DNA. Translation: EAX09580.1.
CH471079 Genomic DNA. Translation: EAX09581.1.
BC063290 mRNA. Translation: AAH63290.1.
CCDSCCDS13678.1. [Q9ULJ3-1]
CCDS42934.1. [Q9ULJ3-2]
RefSeqNP_001091872.1. NM_001098402.1. [Q9ULJ3-1]
NP_001091873.1. NM_001098403.1. [Q9ULJ3-2]
NP_065778.3. NM_020727.4. [Q9ULJ3-1]
XP_005261178.1. XM_005261121.1. [Q9ULJ3-1]
XP_005261180.1. XM_005261123.1. [Q9ULJ3-2]
UniGeneHs.434947.
Hs.618406.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJPNMR-A713-806[»]
ProteinModelPortalQ9ULJ3.
SMRQ9ULJ3. Positions 15-114, 540-600, 664-806, 909-972, 1035-1065.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119066. 6 interactions.
IntActQ9ULJ3. 1 interaction.
MINTMINT-1688321.
STRING9606.ENSP00000308759.

PTM databases

PhosphoSiteQ9ULJ3.

Polymorphism databases

DMDM9979550.

Proteomic databases

MaxQBQ9ULJ3.
PaxDbQ9ULJ3.
PRIDEQ9ULJ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310826; ENSP00000308759; ENSG00000173276. [Q9ULJ3-1]
ENST00000398499; ENSP00000381512; ENSG00000173276. [Q9ULJ3-1]
ENST00000398505; ENSP00000381517; ENSG00000173276. [Q9ULJ3-2]
ENST00000398511; ENSP00000381523; ENSG00000173276. [Q9ULJ3-1]
GeneID49854.
KEGGhsa:49854.
UCSCuc002yzy.4. human. [Q9ULJ3-1]
uc002yzz.4. human. [Q9ULJ3-2]

Organism-specific databases

CTD49854.
GeneCardsGC21M043409.
HGNCHGNC:13083. ZBTB21.
HPAHPA024655.
neXtProtNX_Q9ULJ3.
PharmGKBPA37659.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG303726.
HOGENOMHOG000237324.
HOVERGENHBG056929.
InParanoidQ9ULJ3.
OMADNVLNYI.
OrthoDBEOG7TF789.
PhylomeDBQ9ULJ3.
TreeFamTF331184.

Gene expression databases

ArrayExpressQ9ULJ3.
BgeeQ9ULJ3.
CleanExHS_ZNF295.
GenevestigatorQ9ULJ3.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9ULJ3.
GeneWikiZNF295.
GenomeRNAi49854.
NextBio53012.
PROQ9ULJ3.

Entry information

Entry nameZBT21_HUMAN
AccessionPrimary (citable) accession number: Q9ULJ3
Secondary accession number(s): Q5R2W1, Q5R2W2, Q6P4R0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM