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Q9ULI2 (RIMKB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-citrylglutamate synthase B

EC=6.3.1.17
Alternative name(s):
N-acetyl-aspartylglutamate synthetase B
Short name=NAAG synthetase B
Short name=NAAGS
EC=6.3.2.41
Ribosomal protein S6 modification-like protein B
Gene names
Name:RIMKLB
Synonyms:FAM80B, KIAA1238
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the synthesis of beta-citryl-L-glutamate and N-acetyl-L-aspartyl-L-glutamate. Beta-citryl-L-glutamate is synthesized more efficiently than N-acetyl-L-aspartyl-L-glutamate By similarity.

Catalytic activity

ATP + citrate + L-glutamate = ADP + phosphate + beta-citryl-L-glutamate.

ATP + N-acetyl-L-aspartate + L-glutamate = ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

N-acetyl-L-aspartyl-L-glutamate (NAAG) is the most abundant dipeptide present in vertebrate central nervous system (CNS). Beta-citryl-L-glutamate, a structural analog of NAAG, is present in testis and immature brain By similarity.

Sequence similarities

Belongs to the RimK family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence BAA86552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ULI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ULI2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     282-307: VGFIAFDKACNLDVAGIIADYAASLL → PFQEPKKQIQTNKKIPREKTFLLPPS
     308-386: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Beta-citrylglutamate synthase B
PRO_0000282571

Regions

Domain119 – 304186ATP-grasp
Nucleotide binding193 – 20311ATP By similarity

Sites

Metal binding2641Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 2 By similarity
Metal binding2791Magnesium or manganese 2 By similarity
Binding site1581ATP By similarity
Binding site2191ATP By similarity

Natural variations

Alternative sequence282 – 30726VGFIA…AASLL → PFQEPKKQIQTNKKIPREKT FLLPPS in isoform 2.
VSP_024198
Alternative sequence308 – 38679Missing in isoform 2.
VSP_024199

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: D9C0D47A831AD9D2

FASTA38642,464
        10         20         30         40         50         60 
MCSSVAAKLW FLTDRRIRED YPQKEILRAL KAKCCEEELD FRAVVMDEVV LTIEQGNLGL 

        70         80         90        100        110        120 
RINGELITAY PQVVVVRVPT PWVQSDSDIT VLRHLEKMGC RLMNRPQAIL NCVNKFWTFQ 

       130        140        150        160        170        180 
ELAGHGVPLP DTFSYGGHEN FAKMIDEAEV LEFPMVVKNT RGHRGKAVFL ARDKHHLADL 

       190        200        210        220        230        240 
SHLIRHEAPY LFQKYVKESH GRDVRVIVVG GRVVGTMLRC STDGRMQSNC SLGGVGMMCS 

       250        260        270        280        290        300 
LSEQGKQLAI QVSNILGMDV CGIDLLMKDD GSFCVCEANA NVGFIAFDKA CNLDVAGIIA 

       310        320        330        340        350        360 
DYAASLLPSG RLTRRMSLLS VVSTASETSE PELGPPASTA VDNMSASSSS VDSDPESTER 

       370        380 
ELLTKLPGGL FNMNQLLANE IKLLVD 

« Hide

Isoform 2 [UniParc].

Checksum: 2725103A1A5D627B
Show »

FASTA30734,557

References

[1]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 151-386 (ISOFORM 1).
Tissue: Cervix and Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB033064 mRNA. Translation: BAA86552.1. Different initiation.
AK302847 mRNA. Translation: BAH13820.1.
CH471116 Genomic DNA. Translation: EAW88610.1.
CH471116 Genomic DNA. Translation: EAW88611.1.
BC021977 mRNA. Translation: AAH21977.2.
BC033793 mRNA. Translation: AAH33793.1.
RefSeqNP_065785.2. NM_020734.2.
UniGeneHs.504670.

3D structure databases

ProteinModelPortalQ9ULI2.
SMRQ9ULI2. Positions 7-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121561. 2 interactions.
IntActQ9ULI2. 1 interaction.
STRING9606.ENSP00000350136.

PTM databases

PhosphoSiteQ9ULI2.

Polymorphism databases

DMDM143458798.

Proteomic databases

PaxDbQ9ULI2.
PRIDEQ9ULI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357529; ENSP00000350136; ENSG00000166532. [Q9ULI2-1]
ENST00000535829; ENSP00000445863; ENSG00000166532. [Q9ULI2-1]
ENST00000538135; ENSP00000440943; ENSG00000166532. [Q9ULI2-1]
ENST00000544257; ENSP00000438004; ENSG00000166532. [Q9ULI2-2]
GeneID57494.
KEGGhsa:57494.
UCSCuc001quw.2. human. [Q9ULI2-2]
uc001qux.2. human. [Q9ULI2-1]

Organism-specific databases

CTD57494.
GeneCardsGC12P008851.
HGNCHGNC:29228. RIMKLB.
HPAHPA056344.
HPA059552.
MIM614054. gene.
neXtProtNX_Q9ULI2.
PharmGKBPA164725354.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000043112.
HOVERGENHBG108408.
InParanoidQ9ULI2.
OMATSYPQVV.
OrthoDBEOG7GBFWX.
PhylomeDBQ9ULI2.
TreeFamTF332035.

Gene expression databases

ArrayExpressQ9ULI2.
BgeeQ9ULI2.
CleanExHS_RIMKLB.
GenevestigatorQ9ULI2.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsTIGR00768. rimK_fam. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRIMKLB. human.
GenomeRNAi57494.
NextBio63798.
PROQ9ULI2.
SOURCESearch...

Entry information

Entry nameRIMKB_HUMAN
AccessionPrimary (citable) accession number: Q9ULI2
Secondary accession number(s): B7Z834 expand/collapse secondary AC list , D3DUV2, Q8N4P4, Q8WTW6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM