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Q9ULI2

- RIMKB_HUMAN

UniProt

Q9ULI2 - RIMKB_HUMAN

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Protein

Beta-citrylglutamate synthase B

Gene

RIMKLB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the synthesis of beta-citryl-L-glutamate and N-acetyl-L-aspartyl-L-glutamate. Beta-citryl-L-glutamate is synthesized more efficiently than N-acetyl-L-aspartyl-L-glutamate (By similarity).By similarity

Catalytic activityi

ATP + citrate + L-glutamate = ADP + phosphate + beta-citryl-L-glutamate.
ATP + N-acetyl-L-aspartate + L-glutamate = ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581ATPBy similarity
Binding sitei219 – 2191ATPBy similarity
Metal bindingi264 – 2641Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi277 – 2771Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi277 – 2771Magnesium or manganese 2PROSITE-ProRule annotation
Metal bindingi279 – 2791Magnesium or manganese 2PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi193 – 20311ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. citrate-L-glutamate ligase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
  4. N-acetyl-L-aspartate-L-glutamate ligase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein modification process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-citrylglutamate synthase B (EC:6.3.1.17)
Alternative name(s):
N-acetyl-aspartylglutamate synthetase B (EC:6.3.2.41)
Short name:
NAAG synthetase B
Short name:
NAAGS
Ribosomal protein S6 modification-like protein B
Gene namesi
Name:RIMKLB
Synonyms:FAM80B, KIAA1238
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:29228. RIMKLB.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164725354.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Beta-citrylglutamate synthase BPRO_0000282571Add
BLAST

Proteomic databases

MaxQBiQ9ULI2.
PaxDbiQ9ULI2.
PRIDEiQ9ULI2.

PTM databases

PhosphoSiteiQ9ULI2.

Expressioni

Gene expression databases

BgeeiQ9ULI2.
CleanExiHS_RIMKLB.
ExpressionAtlasiQ9ULI2. baseline and differential.
GenevestigatoriQ9ULI2.

Organism-specific databases

HPAiHPA056344.
HPA059552.

Interactioni

Protein-protein interaction databases

BioGridi121561. 2 interactions.
IntActiQ9ULI2. 1 interaction.
STRINGi9606.ENSP00000350136.

Structurei

3D structure databases

ProteinModelPortaliQ9ULI2.
SMRiQ9ULI2. Positions 92-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 304186ATP-graspPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RimK family.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0189.
GeneTreeiENSGT00390000014577.
HOGENOMiHOG000043112.
HOVERGENiHBG108408.
InParanoidiQ9ULI2.
KOiK18310.
OMAiTSYPQVV.
OrthoDBiEOG7GBFWX.
PhylomeDBiQ9ULI2.
TreeFamiTF332035.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9ULI2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSSVAAKLW FLTDRRIRED YPQKEILRAL KAKCCEEELD FRAVVMDEVV
60 70 80 90 100
LTIEQGNLGL RINGELITAY PQVVVVRVPT PWVQSDSDIT VLRHLEKMGC
110 120 130 140 150
RLMNRPQAIL NCVNKFWTFQ ELAGHGVPLP DTFSYGGHEN FAKMIDEAEV
160 170 180 190 200
LEFPMVVKNT RGHRGKAVFL ARDKHHLADL SHLIRHEAPY LFQKYVKESH
210 220 230 240 250
GRDVRVIVVG GRVVGTMLRC STDGRMQSNC SLGGVGMMCS LSEQGKQLAI
260 270 280 290 300
QVSNILGMDV CGIDLLMKDD GSFCVCEANA NVGFIAFDKA CNLDVAGIIA
310 320 330 340 350
DYAASLLPSG RLTRRMSLLS VVSTASETSE PELGPPASTA VDNMSASSSS
360 370 380
VDSDPESTER ELLTKLPGGL FNMNQLLANE IKLLVD
Length:386
Mass (Da):42,464
Last modified:April 3, 2007 - v2
Checksum:iD9C0D47A831AD9D2
GO
Isoform 2 (identifier: Q9ULI2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-307: VGFIAFDKACNLDVAGIIADYAASLL → PFQEPKKQIQTNKKIPREKTFLLPPS
     308-386: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:307
Mass (Da):34,557
Checksum:i2725103A1A5D627B
GO

Sequence cautioni

The sequence BAA86552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei282 – 30726VGFIA…AASLL → PFQEPKKQIQTNKKIPREKT FLLPPS in isoform 2. 1 PublicationVSP_024198Add
BLAST
Alternative sequencei308 – 38679Missing in isoform 2. 1 PublicationVSP_024199Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB033064 mRNA. Translation: BAA86552.1. Different initiation.
AK302847 mRNA. Translation: BAH13820.1.
CH471116 Genomic DNA. Translation: EAW88610.1.
CH471116 Genomic DNA. Translation: EAW88611.1.
BC021977 mRNA. Translation: AAH21977.2.
BC033793 mRNA. Translation: AAH33793.1.
CCDSiCCDS41748.1. [Q9ULI2-1]
RefSeqiNP_065785.2. NM_020734.3. [Q9ULI2-1]
XP_006719179.1. XM_006719116.1. [Q9ULI2-1]
XP_006719180.1. XM_006719117.1. [Q9ULI2-1]
XP_006719181.1. XM_006719118.1. [Q9ULI2-1]
XP_006719182.1. XM_006719119.1. [Q9ULI2-1]
XP_006719183.1. XM_006719120.1. [Q9ULI2-1]
XP_006719184.1. XM_006719121.1. [Q9ULI2-1]
XP_006719185.1. XM_006719122.1. [Q9ULI2-1]
XP_006719186.1. XM_006719123.1. [Q9ULI2-1]
XP_006719187.1. XM_006719124.1. [Q9ULI2-1]
XP_006719188.1. XM_006719125.1. [Q9ULI2-2]
XP_006719189.1. XM_006719126.1. [Q9ULI2-2]
XP_006719190.1. XM_006719127.1. [Q9ULI2-2]
UniGeneiHs.504670.

Genome annotation databases

EnsembliENST00000357529; ENSP00000350136; ENSG00000166532. [Q9ULI2-1]
ENST00000535829; ENSP00000445863; ENSG00000166532. [Q9ULI2-1]
ENST00000538135; ENSP00000440943; ENSG00000166532. [Q9ULI2-1]
ENST00000544257; ENSP00000438004; ENSG00000166532. [Q9ULI2-2]
ENST00000619374; ENSP00000483871; ENSG00000166532. [Q9ULI2-2]
GeneIDi57494.
KEGGihsa:57494.
UCSCiuc001quw.2. human. [Q9ULI2-2]
uc001qux.2. human. [Q9ULI2-1]

Polymorphism databases

DMDMi143458798.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB033064 mRNA. Translation: BAA86552.1 . Different initiation.
AK302847 mRNA. Translation: BAH13820.1 .
CH471116 Genomic DNA. Translation: EAW88610.1 .
CH471116 Genomic DNA. Translation: EAW88611.1 .
BC021977 mRNA. Translation: AAH21977.2 .
BC033793 mRNA. Translation: AAH33793.1 .
CCDSi CCDS41748.1. [Q9ULI2-1 ]
RefSeqi NP_065785.2. NM_020734.3. [Q9ULI2-1 ]
XP_006719179.1. XM_006719116.1. [Q9ULI2-1 ]
XP_006719180.1. XM_006719117.1. [Q9ULI2-1 ]
XP_006719181.1. XM_006719118.1. [Q9ULI2-1 ]
XP_006719182.1. XM_006719119.1. [Q9ULI2-1 ]
XP_006719183.1. XM_006719120.1. [Q9ULI2-1 ]
XP_006719184.1. XM_006719121.1. [Q9ULI2-1 ]
XP_006719185.1. XM_006719122.1. [Q9ULI2-1 ]
XP_006719186.1. XM_006719123.1. [Q9ULI2-1 ]
XP_006719187.1. XM_006719124.1. [Q9ULI2-1 ]
XP_006719188.1. XM_006719125.1. [Q9ULI2-2 ]
XP_006719189.1. XM_006719126.1. [Q9ULI2-2 ]
XP_006719190.1. XM_006719127.1. [Q9ULI2-2 ]
UniGenei Hs.504670.

3D structure databases

ProteinModelPortali Q9ULI2.
SMRi Q9ULI2. Positions 92-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121561. 2 interactions.
IntActi Q9ULI2. 1 interaction.
STRINGi 9606.ENSP00000350136.

PTM databases

PhosphoSitei Q9ULI2.

Polymorphism databases

DMDMi 143458798.

Proteomic databases

MaxQBi Q9ULI2.
PaxDbi Q9ULI2.
PRIDEi Q9ULI2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000357529 ; ENSP00000350136 ; ENSG00000166532 . [Q9ULI2-1 ]
ENST00000535829 ; ENSP00000445863 ; ENSG00000166532 . [Q9ULI2-1 ]
ENST00000538135 ; ENSP00000440943 ; ENSG00000166532 . [Q9ULI2-1 ]
ENST00000544257 ; ENSP00000438004 ; ENSG00000166532 . [Q9ULI2-2 ]
ENST00000619374 ; ENSP00000483871 ; ENSG00000166532 . [Q9ULI2-2 ]
GeneIDi 57494.
KEGGi hsa:57494.
UCSCi uc001quw.2. human. [Q9ULI2-2 ]
uc001qux.2. human. [Q9ULI2-1 ]

Organism-specific databases

CTDi 57494.
GeneCardsi GC12P008851.
HGNCi HGNC:29228. RIMKLB.
HPAi HPA056344.
HPA059552.
MIMi 614054. gene.
neXtProti NX_Q9ULI2.
PharmGKBi PA164725354.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0189.
GeneTreei ENSGT00390000014577.
HOGENOMi HOG000043112.
HOVERGENi HBG108408.
InParanoidi Q9ULI2.
KOi K18310.
OMAi TSYPQVV.
OrthoDBi EOG7GBFWX.
PhylomeDBi Q9ULI2.
TreeFami TF332035.

Miscellaneous databases

ChiTaRSi RIMKLB. human.
GenomeRNAii 57494.
NextBioi 63798.
PROi Q9ULI2.
SOURCEi Search...

Gene expression databases

Bgeei Q9ULI2.
CleanExi HS_RIMKLB.
ExpressionAtlasi Q9ULI2. baseline and differential.
Genevestigatori Q9ULI2.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view ]
Pfami PF08443. RimK. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00768. rimK_fam. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
    DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 151-386 (ISOFORM 1).
    Tissue: Cervix and Ovary.

Entry informationi

Entry nameiRIMKB_HUMAN
AccessioniPrimary (citable) accession number: Q9ULI2
Secondary accession number(s): B7Z834
, D3DUV2, Q8N4P4, Q8WTW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

N-acetyl-L-aspartyl-L-glutamate (NAAG) is the most abundant dipeptide present in vertebrate central nervous system (CNS). Beta-citryl-L-glutamate, a structural analog of NAAG, is present in testis and immature brain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3