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Protein

DENN domain-containing protein 2A

Gene

DENND2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May play a role in late endosomes back to trans-Golgi network/TGN transport.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • positive regulation of GTPase activity Source: GOC
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to Golgi Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 2A
Gene namesi
Name:DENND2A
Synonyms:KIAA1277
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22212. DENND2A.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • cytosol Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134931054.

Polymorphism and mutation databases

BioMutaiDENND2A.
DMDMi296439469.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10091009DENN domain-containing protein 2APRO_0000242682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei551 – 5511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9ULE3.
PaxDbiQ9ULE3.
PRIDEiQ9ULE3.

PTM databases

iPTMnetiQ9ULE3.
PhosphoSiteiQ9ULE3.
SwissPalmiQ9ULE3.

Expressioni

Gene expression databases

BgeeiQ9ULE3.
CleanExiHS_DENND2A.
ExpressionAtlasiQ9ULE3. baseline and differential.
GenevisibleiQ9ULE3. HS.

Organism-specific databases

HPAiHPA021778.
HPA026718.

Interactioni

Protein-protein interaction databases

BioGridi118031. 2 interactions.
IntActiQ9ULE3. 1 interaction.
MINTiMINT-7969687.
STRINGi9606.ENSP00000275884.

Structurei

3D structure databases

ProteinModelPortaliQ9ULE3.
SMRiQ9ULE3. Positions 598-962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini577 – 65377UDENNAdd
BLAST
Domaini660 – 844185DENNPROSITE-ProRule annotationAdd
BLAST
Domaini895 – 96268dDENNPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3569. Eukaryota.
ENOG410XT3N. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000273875.
HOVERGENiHBG079980.
InParanoidiQ9ULE3.
OMAiQTFRGFI.
OrthoDBiEOG79GT6H.
PhylomeDBiQ9ULE3.
TreeFamiTF320336.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ULE3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMFSLDMII SDPAAEASRA GKKQLRGVQN PCPSARARPR HKSLNIKDKI
60 70 80 90 100
SEWEGKKEVP TPAPSRRADG QEDYLPSSTV ERRSSDGVRT QVTEAKNGMR
110 120 130 140 150
PGTESTEKER NKGAVNVGGQ DPEPGQDLSQ PEREVDPSWG RGREPRLGKL
160 170 180 190 200
RFQNDPLSVL KQVKKLEQAL KDGSAGLDPQ LPGTCYSPHC PPDKAEAGST
210 220 230 240 250
LPENLGGGSG SEVSQRVHPS DLEGREPTPE LVEDRKGSCR RPWDRSLENV
260 270 280 290 300
YRGSEGSPTK PFINPLPKPR RTFKHAGEGD KDGKPGIGFR KEKRNLPPLP
310 320 330 340 350
SLPPPPLPSS PPPSSVNRRL WTGRQKSSAD HRKSYEFEDL LQSSSESSRV
360 370 380 390 400
DWYAQTKLGL TRTLSEENVY EDILDPPMKE NPYEDIELHG RCLGKKCVLN
410 420 430 440 450
FPASPTSSIP DTLTKQSLSK PAFFRQNSER RNFKLLDTRK LSRDGTGSPS
460 470 480 490 500
KISPPSTPSS PDDIFFNLGD PQNGRKKRKI PKLVLRINAI YEVRRGKKRV
510 520 530 540 550
KRLSQSMESN SGKVTDENSE SDSDTEEKLK AHSQRLVNVK SRLKQAPRYP
560 570 580 590 600
SLARELIEYQ ERQLFEYFVV VSLHKKQAGA AYVPELTQQF PLKLERSFKF
610 620 630 640 650
MREAEDQLKA IPQFCFPDAK DWVPVQQFTS ETFSFVLTGE DGSRRFGYCR
660 670 680 690 700
RLLPGGKGKR LPEVYCIVSR LGCFSLFSRI LDEVEKRRGI SPALVQPLMR
710 720 730 740 750
SVMEAPFPAL GKTILVKNFL PGSGTEVIEL CRPLDSRLEH VDFESLFSSL
760 770 780 790 800
SVRHLVCVFA SLLLERRVIF IADKLSILSK CCHAMVALIY PFAWQHTYIP
810 820 830 840 850
VLPPAMVDIV CSPTPFLIGL LSSSLPLLRE LPLEEVLVVD LVNSRFLRQM
860 870 880 890 900
DDEDSILPRK LQVALEHILE QRNELACEQD EGPLDGRHGP ESSPLNEVVS
910 920 930 940 950
EAFVRFFVEI VGHYSLFLTS GEREERTLQR EAFRKAVSSK SLRHFLEVFM
960 970 980 990 1000
ETQMFRGFIQ ERELRRQDAK GLFEVRAQEY LETLPSGEHS GVNKFLKGLG

NKMKFLHKK
Length:1,009
Mass (Da):113,853
Last modified:May 18, 2010 - v4
Checksum:i4A596E6999F8AF3E
GO
Isoform 2 (identifier: Q9ULE3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     776-800: SILSKCCHAMVALIYPFAWQHTYIP → RYPPWLLLLKRLNDSRSWTL
     801-1009: Missing.

Note: No experimental confirmation available.
Show »
Length:795
Mass (Da):89,514
Checksum:i945B7C1E37FB287B
GO

Sequence cautioni

The sequence BAA86591.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti600 – 6001F → L in AAH49193 (PubMed:15489334).Curated
Sequence conflicti864 – 8641A → V in BAA86591 (PubMed:10574462).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti156 – 1561P → H.2 Publications
Corresponds to variant rs269243 [ dbSNP | Ensembl ].
VAR_026856
Natural varianti729 – 7291E → K.
Corresponds to variant rs2293177 [ dbSNP | Ensembl ].
VAR_026857
Natural varianti777 – 7771I → T.
Corresponds to variant rs6464833 [ dbSNP | Ensembl ].
VAR_026858

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei776 – 80025SILSK…HTYIP → RYPPWLLLLKRLNDSRSWTL in isoform 2. 1 PublicationVSP_019466Add
BLAST
Alternative sequencei801 – 1009209Missing in isoform 2. 1 PublicationVSP_019467Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033103 mRNA. Translation: BAA86591.2. Different initiation.
AC006452 Genomic DNA. No translation available.
AC069335 Genomic DNA. No translation available.
BC049193 mRNA. Translation: AAH49193.1.
BC115004 mRNA. Translation: AAI15005.1.
CCDSiCCDS43659.1. [Q9ULE3-1]
RefSeqiNP_001304981.1. NM_001318052.1. [Q9ULE3-1]
NP_001304982.1. NM_001318053.1. [Q9ULE3-2]
NP_056504.3. NM_015689.4. [Q9ULE3-1]
XP_005250034.1. XM_005249977.2. [Q9ULE3-1]
XP_005250035.1. XM_005249978.3. [Q9ULE3-1]
XP_011514354.1. XM_011516052.1. [Q9ULE3-1]
XP_011514355.1. XM_011516053.1. [Q9ULE3-1]
UniGeneiHs.6385.

Genome annotation databases

EnsembliENST00000275884; ENSP00000275884; ENSG00000146966. [Q9ULE3-1]
ENST00000492720; ENSP00000419464; ENSG00000146966. [Q9ULE3-2]
ENST00000496613; ENSP00000419654; ENSG00000146966. [Q9ULE3-1]
ENST00000537639; ENSP00000442245; ENSG00000146966. [Q9ULE3-1]
GeneIDi27147.
KEGGihsa:27147.
UCSCiuc003vvw.3. human. [Q9ULE3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033103 mRNA. Translation: BAA86591.2. Different initiation.
AC006452 Genomic DNA. No translation available.
AC069335 Genomic DNA. No translation available.
BC049193 mRNA. Translation: AAH49193.1.
BC115004 mRNA. Translation: AAI15005.1.
CCDSiCCDS43659.1. [Q9ULE3-1]
RefSeqiNP_001304981.1. NM_001318052.1. [Q9ULE3-1]
NP_001304982.1. NM_001318053.1. [Q9ULE3-2]
NP_056504.3. NM_015689.4. [Q9ULE3-1]
XP_005250034.1. XM_005249977.2. [Q9ULE3-1]
XP_005250035.1. XM_005249978.3. [Q9ULE3-1]
XP_011514354.1. XM_011516052.1. [Q9ULE3-1]
XP_011514355.1. XM_011516053.1. [Q9ULE3-1]
UniGeneiHs.6385.

3D structure databases

ProteinModelPortaliQ9ULE3.
SMRiQ9ULE3. Positions 598-962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118031. 2 interactions.
IntActiQ9ULE3. 1 interaction.
MINTiMINT-7969687.
STRINGi9606.ENSP00000275884.

PTM databases

iPTMnetiQ9ULE3.
PhosphoSiteiQ9ULE3.
SwissPalmiQ9ULE3.

Polymorphism and mutation databases

BioMutaiDENND2A.
DMDMi296439469.

Proteomic databases

MaxQBiQ9ULE3.
PaxDbiQ9ULE3.
PRIDEiQ9ULE3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000275884; ENSP00000275884; ENSG00000146966. [Q9ULE3-1]
ENST00000492720; ENSP00000419464; ENSG00000146966. [Q9ULE3-2]
ENST00000496613; ENSP00000419654; ENSG00000146966. [Q9ULE3-1]
ENST00000537639; ENSP00000442245; ENSG00000146966. [Q9ULE3-1]
GeneIDi27147.
KEGGihsa:27147.
UCSCiuc003vvw.3. human. [Q9ULE3-1]

Organism-specific databases

CTDi27147.
GeneCardsiDENND2A.
H-InvDBHIX0007143.
HGNCiHGNC:22212. DENND2A.
HPAiHPA021778.
HPA026718.
neXtProtiNX_Q9ULE3.
PharmGKBiPA134931054.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3569. Eukaryota.
ENOG410XT3N. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000273875.
HOVERGENiHBG079980.
InParanoidiQ9ULE3.
OMAiQTFRGFI.
OrthoDBiEOG79GT6H.
PhylomeDBiQ9ULE3.
TreeFamiTF320336.

Miscellaneous databases

ChiTaRSiDENND2A. human.
GenomeRNAii27147.
PROiQ9ULE3.

Gene expression databases

BgeeiQ9ULE3.
CleanExiHS_DENND2A.
ExpressionAtlasiQ9ULE3. baseline and differential.
GenevisibleiQ9ULE3. HS.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
    DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT HIS-156.
    Tissue: Brain.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 372-1009 (ISOFORM 1), VARIANT HIS-156.
    Tissue: Embryonic stem cell and Liver.
  4. "Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
    Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
    J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDEN2A_HUMAN
AccessioniPrimary (citable) accession number: Q9ULE3
Secondary accession number(s): C9JUI3, Q1RMD5, Q86XY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 18, 2010
Last modified: June 8, 2016
This is version 96 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.