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Protein

Transcription factor-like 5 protein

Gene

TCFL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.1 Publication

Miscellaneous

Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune disease caused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 and R3 epitopes, which are recognized by T- and B-cells, respectively.

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • protein dimerization activity Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • regulation of cell differentiation Source: UniProtKB
  • regulation of cell proliferation Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • spermatogenesis Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: ProtInc

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ9UL49.
SIGNORiQ9UL49.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor-like 5 protein
Alternative name(s):
Cha transcription factor
HPV-16 E2-binding protein 1
Short name:
E2BP-1
Gene namesi
Name:TCFL5
Synonyms:CHA, E2BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:11646. TCFL5.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • male germ cell nucleus Source: Ensembl
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10732.
OpenTargetsiENSG00000101190.
PharmGKBiPA36398.

Polymorphism and mutation databases

BioMutaiTCFL5.
DMDMi166214983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274751 – 500Transcription factor-like 5 proteinAdd BLAST500

Proteomic databases

MaxQBiQ9UL49.
PaxDbiQ9UL49.
PeptideAtlasiQ9UL49.
PRIDEiQ9UL49.

PTM databases

iPTMnetiQ9UL49.
PhosphoSitePlusiQ9UL49.

Expressioni

Tissue specificityi

Isoform 3 is testis specific. Isoform 2 is pancreas specific.1 Publication

Developmental stagei

Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.1 Publication

Gene expression databases

BgeeiENSG00000101190.
CleanExiHS_TCFL5.
ExpressionAtlasiQ9UL49. baseline and differential.
GenevisibleiQ9UL49. HS.

Organism-specific databases

HPAiHPA055223.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115955. 1 interactor.
STRINGi9606.ENSP00000334294.

Structurei

3D structure databases

ProteinModelPortaliQ9UL49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini400 – 450bHLHPROSITE-ProRule annotationAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni347 – 356R3 epitope (recognized by Chagas's antibodies)10
Regioni481 – 500R1 epitope (recognized by Chagas's antibodies)Add BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 182Ala-richAdd BLAST116

Phylogenomic databases

eggNOGiENOG410IECS. Eukaryota.
ENOG410YEAA. LUCA.
GeneTreeiENSGT00390000002821.
HOGENOMiHOG000231268.
HOVERGENiHBG067309.
InParanoidiQ9UL49.
KOiK09092.
OMAiDRFNSIQ.
OrthoDBiEOG091G0G81.
PhylomeDBiQ9UL49.
TreeFamiTF336112.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q9UL49-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGPGPREPP PEAGAAGGEA AVEGAGGGDA ALGEPGLSFT TTDLSLVEMT
60 70 80 90 100
EVEYTQLQHI LCSHMEAAAD GELETRLNSA LLAAAGPGAG AGGFAAGGQG
110 120 130 140 150
GAAPVYPVLC PSALAADAPC LGHIDFQELR MMLLSEAGAA EKTSGGGDGA
160 170 180 190 200
RARADGAAKE GAGAAAAAAG PDGAPEARAK PAVRVRLEDR FNSIPAEPPP
210 220 230 240 250
APRGPEPPEP GGALNNLVTL IRHPSELMNV PLQQQNKCTA LVKNKTAATT
260 270 280 290 300
TALQFTYPLF TTNACSTSGN SNLSQTQSSS NSCSVLEAAK HQDIGLPRAF
310 320 330 340 350
SFCYQQEIES TKQTLGSRNK VLPEQVWIKV GEAALCKQAL KRNRSRMRQL
360 370 380 390 400
DTNVERRALG EIQNVGEGAT ATQGAWQSSE SSQANLGEQA QSGPQGGRSQ
410 420 430 440 450
RRERHNRMER DRRRRIRICC DELNLLVPFC NAETDKATTL QWTTAFLKYI
460 470 480 490 500
QERHGDSLKK EFESVFCGKT GRRLKLTRPD SLVTCPAQGS LQSSPSMEIK
Length:500
Mass (Da):52,697
Last modified:January 15, 2008 - v2
Checksum:i39918DCA21A94E7B
GO
Isoform 1 (identifier: Q9UL49-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:452
Mass (Da):48,263
Checksum:iB501EF5BCE12B5A8
GO
Isoform 2 (identifier: Q9UL49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-227: Missing.

Show »
Length:273
Mass (Da):30,479
Checksum:iC2811B004570E76A
GO

Sequence cautioni

The sequence BAA36557 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75T → M in BAA36557 (PubMed:9763657).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061263272N → D. Corresponds to variant dbSNP:rs17854409Ensembl.1
Natural variantiVAR_049555380E → D. Corresponds to variant dbSNP:rs34304654Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0021601 – 227Missing in isoform 2. 1 PublicationAdd BLAST227
Alternative sequenceiVSP_0303101 – 48Missing in isoform 1. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271337 mRNA. Translation: CAC24700.1.
AF070992 mRNA. Translation: AAD53986.1.
AL035669 Genomic DNA. Translation: CAC12752.2.
AB012124 mRNA. Translation: BAA36557.1. Different initiation.
CCDSiCCDS13506.1. [Q9UL49-3]
RefSeqiNP_006593.2. NM_006602.3. [Q9UL49-3]
UniGeneiHs.126248.

Genome annotation databases

EnsembliENST00000335351; ENSP00000334294; ENSG00000101190. [Q9UL49-3]
GeneIDi10732.
KEGGihsa:10732.
UCSCiuc002ydp.3. human. [Q9UL49-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTCFL5_HUMAN
AccessioniPrimary (citable) accession number: Q9UL49
Secondary accession number(s): O94771, Q9BYW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: January 15, 2008
Last modified: May 10, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot