Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor-like 5 protein

Gene

TCFL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. regulation of cell differentiation Source: UniProtKB
  4. regulation of cell proliferation Source: UniProtKB
  5. regulation of transcription, DNA-templated Source: UniProtKB
  6. spermatogenesis Source: UniProtKB
  7. transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ9UL49.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor-like 5 protein
Alternative name(s):
Cha transcription factor
HPV-16 E2-binding protein 1
Short name:
E2BP-1
Gene namesi
Name:TCFL5
Synonyms:CHA, E2BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:11646. TCFL5.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. male germ cell nucleus Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36398.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Transcription factor-like 5 proteinPRO_0000127475Add
BLAST

Proteomic databases

MaxQBiQ9UL49.
PaxDbiQ9UL49.
PRIDEiQ9UL49.

PTM databases

PhosphoSiteiQ9UL49.

Expressioni

Tissue specificityi

Isoform 3 is testis specific. Isoform 2 is pancreas specific.1 Publication

Developmental stagei

Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.1 Publication

Gene expression databases

BgeeiQ9UL49.
CleanExiHS_TCFL5.
ExpressionAtlasiQ9UL49. baseline and differential.
GenevestigatoriQ9UL49.

Organism-specific databases

HPAiHPA055223.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.

Protein-protein interaction databases

BioGridi115955. 1 interaction.
STRINGi9606.ENSP00000334294.

Structurei

3D structure databases

ProteinModelPortaliQ9UL49.
SMRiQ9UL49. Positions 398-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini400 – 45051bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni347 – 35610R3 epitope (recognized by Chagas's antibodies)
Regioni481 – 50020R1 epitope (recognized by Chagas's antibodies)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi67 – 182116Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG28448.
GeneTreeiENSGT00390000002821.
HOGENOMiHOG000231268.
HOVERGENiHBG067309.
InParanoidiQ9UL49.
KOiK09092.
OMAiDRFNSIQ.
OrthoDBiEOG7CK36S.
PhylomeDBiQ9UL49.
TreeFamiTF336112.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 3 (identifier: Q9UL49-3) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGPGPREPP PEAGAAGGEA AVEGAGGGDA ALGEPGLSFT TTDLSLVEMT
60 70 80 90 100
EVEYTQLQHI LCSHMEAAAD GELETRLNSA LLAAAGPGAG AGGFAAGGQG
110 120 130 140 150
GAAPVYPVLC PSALAADAPC LGHIDFQELR MMLLSEAGAA EKTSGGGDGA
160 170 180 190 200
RARADGAAKE GAGAAAAAAG PDGAPEARAK PAVRVRLEDR FNSIPAEPPP
210 220 230 240 250
APRGPEPPEP GGALNNLVTL IRHPSELMNV PLQQQNKCTA LVKNKTAATT
260 270 280 290 300
TALQFTYPLF TTNACSTSGN SNLSQTQSSS NSCSVLEAAK HQDIGLPRAF
310 320 330 340 350
SFCYQQEIES TKQTLGSRNK VLPEQVWIKV GEAALCKQAL KRNRSRMRQL
360 370 380 390 400
DTNVERRALG EIQNVGEGAT ATQGAWQSSE SSQANLGEQA QSGPQGGRSQ
410 420 430 440 450
RRERHNRMER DRRRRIRICC DELNLLVPFC NAETDKATTL QWTTAFLKYI
460 470 480 490 500
QERHGDSLKK EFESVFCGKT GRRLKLTRPD SLVTCPAQGS LQSSPSMEIK
Length:500
Mass (Da):52,697
Last modified:January 15, 2008 - v2
Checksum:i39918DCA21A94E7B
GO
Isoform 1 (identifier: Q9UL49-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:452
Mass (Da):48,263
Checksum:iB501EF5BCE12B5A8
GO
Isoform 2 (identifier: Q9UL49-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-227: Missing.

Show »
Length:273
Mass (Da):30,479
Checksum:iC2811B004570E76A
GO

Sequence cautioni

The sequence BAA36557.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751T → M in BAA36557. (PubMed:9763657)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti272 – 2721N → D.
Corresponds to variant rs17854409 [ dbSNP | Ensembl ].
VAR_061263
Natural varianti380 – 3801E → D.
Corresponds to variant rs34304654 [ dbSNP | Ensembl ].
VAR_049555

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 227227Missing in isoform 2. 1 PublicationVSP_002160Add
BLAST
Alternative sequencei1 – 4848Missing in isoform 1. CuratedVSP_030310Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271337 mRNA. Translation: CAC24700.1.
AF070992 mRNA. Translation: AAD53986.1.
AL035669 Genomic DNA. Translation: CAC12752.2.
AB012124 mRNA. Translation: BAA36557.1. Different initiation.
CCDSiCCDS13506.1. [Q9UL49-3]
RefSeqiNP_006593.2. NM_006602.3. [Q9UL49-3]
UniGeneiHs.126248.

Genome annotation databases

EnsembliENST00000335351; ENSP00000334294; ENSG00000101190. [Q9UL49-3]
GeneIDi10732.
KEGGihsa:10732.
UCSCiuc002ydo.3. human. [Q9UL49-3]

Polymorphism databases

DMDMi166214983.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271337 mRNA. Translation: CAC24700.1.
AF070992 mRNA. Translation: AAD53986.1.
AL035669 Genomic DNA. Translation: CAC12752.2.
AB012124 mRNA. Translation: BAA36557.1. Different initiation.
CCDSiCCDS13506.1. [Q9UL49-3]
RefSeqiNP_006593.2. NM_006602.3. [Q9UL49-3]
UniGeneiHs.126248.

3D structure databases

ProteinModelPortaliQ9UL49.
SMRiQ9UL49. Positions 398-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115955. 1 interaction.
STRINGi9606.ENSP00000334294.

PTM databases

PhosphoSiteiQ9UL49.

Polymorphism databases

DMDMi166214983.

Proteomic databases

MaxQBiQ9UL49.
PaxDbiQ9UL49.
PRIDEiQ9UL49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335351; ENSP00000334294; ENSG00000101190. [Q9UL49-3]
GeneIDi10732.
KEGGihsa:10732.
UCSCiuc002ydo.3. human. [Q9UL49-3]

Organism-specific databases

CTDi10732.
GeneCardsiGC20M061472.
H-InvDBHIX0203065.
HGNCiHGNC:11646. TCFL5.
HPAiHPA055223.
MIMi604745. gene.
neXtProtiNX_Q9UL49.
PharmGKBiPA36398.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG28448.
GeneTreeiENSGT00390000002821.
HOGENOMiHOG000231268.
HOVERGENiHBG067309.
InParanoidiQ9UL49.
KOiK09092.
OMAiDRFNSIQ.
OrthoDBiEOG7CK36S.
PhylomeDBiQ9UL49.
TreeFamiTF336112.

Enzyme and pathway databases

SignaLinkiQ9UL49.

Miscellaneous databases

ChiTaRSiTCFL5. human.
GenomeRNAii10732.
NextBioi40740.
PROiQ9UL49.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UL49.
CleanExiHS_TCFL5.
ExpressionAtlasiQ9UL49. baseline and differential.
GenevestigatoriQ9UL49.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dominant T- and B-cell epitopes in an autoantigen linked to Chagas' disease."
    Girones N., Rodriguez C.I., Carrasco-Marin E., Hernaez R.F., de Rego J.L., Fresno M.
    J. Clin. Invest. 107:985-993(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INVOLVEMENT IN AUTOIMMUNE DISEASE.
    Tissue: T lymphoblast.
  2. Zheng P.-S., Pater A.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage."
    Maruyama O., Nishimori H., Katagiri T., Miki Y., Ueno A., Nakamura Y.
    Cytogenet. Cell Genet. 82:41-45(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-500 (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiTCFL5_HUMAN
AccessioniPrimary (citable) accession number: Q9UL49
Secondary accession number(s): O94771, Q9BYW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: January 15, 2008
Last modified: January 7, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune disease caused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 and R3 epitopes, which are recognized by T- and B-cells, respectively.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.