Q9UL25 (RAB21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-21

Gene names

Name:	RAB21
Synonyms:	KIAA0118

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration By similarity. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Ref.6
Subunit structure	Interacts with the cytoplasmic tail of integrins ITGA1, ITGA2, ITGA5, ITGA6, ITGA11 and ITGB1 By similarity. Interacts with RABGEF1 (via VPS9 domain).
Subcellular location	Endoplasmic reticulum membrane; Lipid-anchor By similarity. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. Note: In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells. Ref.1 Ref.5 Ref.6
Tissue specificity	Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression. Ref.1
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cleavage furrow Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GDP binding Inferred from direct assay Ref.9. Source: UniProtKB GTP binding Inferred from direct assay Ref.9. Source: UniProtKB GTPase activity Inferred from direct assay Ref.9. Source: UniProtKB protein binding Inferred from physical interaction Ref.10. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 222

221

Ras-related protein Rab-21

PRO_0000121205

Propeptide

223 – 225

3

Removed in mature form Potential

PRO_0000370768

Regions

Nucleotide binding

26 – 34

9

GTP

Nucleotide binding

74 – 78

5

GTP By similarity

Nucleotide binding

132 – 135

4

GTP

Nucleotide binding

162 – 164

3

GTP

Motif

48 – 56

9

Effector region By similarity

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.3 Ref.7

Modified residue

222

1

Cysteine methyl ester Potential

Lipidation

221

1

S-geranylgeranyl cysteine By similarity

Lipidation

222

1

S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis

33

1

T → N: Defects in GTP-binding. Ref.5

Mutagenesis

78

1

Q → L: Defects in GTP hydrolysis. Ref.5

Secondary structure

1

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225

Helix Strand Turn

Details...

Beta strand

18 – 25

8

Helix

32 – 41

10

Beta strand

55 – 63

9

Beta strand

66 – 74

9

Beta strand

93 – 100

8

Helix

104 – 121

18

Helix

122 – 124

3

Beta strand

125 – 132

8

Helix

134 – 139

6

Helix

144 – 153

10

Beta strand

157 – 160

4

Turn

163 – 166

4

Helix

169 – 182

14

Sequences

	Sequence		Length	Mass (Da)
	Q9UL25 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 73CA2C127F0CBFD6	FASTA	225	24,348
	10 20 30 40 50 60 MAAAGGGGGG AAAAGRAYSF KVVLLGEGCV GKTSLVLRYC ENKFNDKHIT TLQASFLTKK 70 80 90 100 110 120 LNIGGKRVNL AIWDTAGQER FHALGPIYYR DSNGAILVYD ITDEDSFQKV KNWVKELRKM 130 140 150 160 170 180 LGNEICLCIV GNKIDLEKER HVSIQEAESY AESVGAKHYH TSAKQNKGIE ELFLDLCKRM 190 200 210 220 IETAQVDERA KGNGSSQPGT ARRGVQIIDD EPQAQTSGGG CCSSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells." Opdam F.J.M., Kamps G., Croes H., van Bokhoven H., Ginsel L.A., Fransen J.A.M. Eur. J. Cell Biol. 79:308-316(2000) [PubMed: 10887961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Colon carcinoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph and Placenta.
[3]	Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-16; 48-59; 91-109 AND 141-157, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[4]	"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-225. Tissue: Bone marrow.
[5]	"A role for the small GTPase Rab21 in the early endocytic pathway." Simpson J.C., Griffiths G., Wessling-Resnick M., Fransen J.A.M., Bennett H., Jones A.T. J. Cell Sci. 117:6297-6311(2004) [PubMed: 15561770] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF THR-33 AND GLN-78.
[6]	"Integrin trafficking regulated by Rab21 is necessary for cytokinesis." Pellinen T., Tuomi S., Arjonen A., Wolf M., Edgren H., Meyer H., Grosse R., Kitzing T., Rantala J.K., Kallioniemi O., Faessler R., Kallio M., Ivaska J. Dev. Cell 15:371-385(2008) [PubMed: 18804435] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Structural basis of family-wide Rab GTPase recognition by rabenosyn-5." Eathiraj S., Pan X., Ritacco C., Lambright D.G. Nature 436:415-419(2005) [PubMed: 16034420] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 16-183 IN COMPLEX WITH GTP ANALOG AND GDP.
[10]	"Structural basis for Rab GTPase activation by VPS9 domain exchange factors." Delprato A., Lambright D.G. Nat. Struct. Mol. Biol. 14:406-412(2007) [PubMed: 17450153] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 16-183 IN COMPLEX WITH RABGEF1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF091035 mRNA. Translation: AAF00048.1.
BC021901 mRNA. Translation: AAH21901.1.
BC092475 mRNA. Translation: AAH92475.1.
D42087 mRNA. Translation: BAA07682.1.

IPI

IPI00007755.

RefSeq

NP_055814.1. NM_014999.2.

UniGene

Hs.524590.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YZT	X-ray	2.05	A/B	16-183	[»]
1YZU	X-ray	2.50	A/B	16-183	[»]
1Z08	X-ray	1.80	A/B/C/D	16-183	[»]
1Z0I	X-ray	2.33	A	16-183	[»]
2OT3	X-ray	2.10	B	16-183	[»]

ProteinModelPortal

Q9UL25.

SMR

Q9UL25. Positions 17-181.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29349N.

IntAct

Q9UL25. 1 interaction.

MINT

MINT-3081967.

STRING

Q9UL25.

PTM databases

PhosphoSite

Q9UL25.

Polymorphism databases

DMDM

13633613.

Proteomic databases

PeptideAtlas

Q9UL25.

PRIDE

Q9UL25.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000261263; ENSP00000261263; ENSG00000080371.

GeneID

23011.

KEGG

hsa:23011.

UCSC

uc001swt.1. human.

Organism-specific databases

CTD

23011.

GeneCards

GC12P072100.

H-InvDB

HIX0010822.

HGNC

HGNC:18263. RAB21.

HPA

HPA016988.

MIM

612398. gene.

neXtProt

NX_Q9UL25.

PharmGKB

PA34111.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG11156.

GeneTree

ENSGT00550000074186.

HOGENOM

HBG745225.

HOVERGEN

HBG009351.

InParanoid

Q9UL25.

OMA

EPQAQTS.

OrthoDB

EOG4W0XF0.

PhylomeDB

Q9UL25.

Enzyme and pathway databases

BRENDA

3.6.5.2. 2681.

Gene expression databases

ArrayExpress

Q9UL25.

Bgee

Q9UL25.

CleanEx

HS_RAB21.

Genevestigator

Q9UL25.

GermOnline

ENSG00000080371. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

KO

K07890.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

43928.

SOURCE

Search...

Entry information

Entry name

RAB21_HUMAN

Accession

Primary (citable) accession number: Q9UL25
Secondary accession number(s): Q14466, Q569H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.