Q9UL19 (HRSL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinoic acid receptor responder protein 3
EC=3.1.1.-

Alternative name(s):
HRAS-like suppressor 4
Short name=HRSL4
RAR-responsive protein TIG3
Retinoid-inducible gene 1 protein
Tazarotene-induced gene 3 protein

Gene names

Name:	RARRES3
Synonyms:	RIG1, TIG3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. N- and O-acylation activity is hardly detectable. Ref.3
Subcellular location	Membrane; Single-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Ref.2 Ref.3
Induction	By all-trans-retinoic acid and synthetic retinoids. Ref.2
Sequence similarities	Belongs to the H-rev107 family.
Biophysicochemical properties	Kinetic parameters: KM=400 µM for dipalmitoyl-PC Ref.3 Vmax=530 nmol/min/mg enzyme with dipalmitoyl-PC as substrate Vmax=240 nmol/min/mg enzyme with dipalmitoyl-PE as substrate pH dependence: Optimum pH is 8.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc phospholipid metabolic process Inferred from direct assay PubMed 20100577. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	phospholipase A2 activity Inferred from direct assay PubMed 20100577. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UL19-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UL19-2)

The sequence of this isoform differs from the canonical sequence as follows:
     131-164: EKAKVEVGVATALGILVVAGCSFAIRRYQKKATA → RTSLDNPSPSAWGWTHGAVQPGDHCE

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 164

164

Retinoic acid receptor responder protein 3

PRO_0000152490

Regions

Topological domain

1 – 133

133

Cytoplasmic Potential

Transmembrane

134 – 155

22

Helical; Potential

Topological domain

156 – 164

9

Lumenal Potential

Natural variations

Alternative sequence

131 – 164

34

EKAKV…KKATA → RTSLDNPSPSAWGWTHGAVQ PGDHCE in isoform 2.

VSP_041496

Natural variant

69

1

V → L.
Corresponds to variant rs35502888 [ dbSNP | Ensembl ].

VAR_049480

Natural variant

162

1

A → V.
Corresponds to variant rs35845275 [ dbSNP | Ensembl ].

VAR_049481

Experimental info

Sequence conflict

44

1

G → R in BAG56873. Ref.5

Sequence conflict

63

1

E → G in AAC84000. Ref.1

Sequence conflict

118

1

T → A in AAC84000. Ref.1

Secondary structure

1

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164

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

13 – 17

5

Beta strand

19 – 21

3

Beta strand

23 – 29

7

Beta strand

32 – 37

6

Beta strand

50 – 52

3

Beta strand

58 – 64

7

Helix

65 – 69

5

Beta strand

73 – 76

4

Helix

79 – 83

5

Helix

89 – 99

11

Helix

109 – 121

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 95625B8FD375FD38	FASTA	164	18,179
	10 20 30 40 50 60 MASPHQEPKP GDLIEIFRLG YEHWALYIGD GYVIHLAPPS EYPGAGSSSV FSVLSNSAEV 70 80 90 100 110 120 KRERLEDVVG GCCYRVNNSL DHEYQPRPVE VIISSAKEMV GQKMKYSIVS RNCEHFVTQL 130 140 150 160 RYGKSRCKQV EKAKVEVGVA TALGILVVAG CSFAIRRYQK KATA
	Isoform 2 [UniParc]. Checksum: C9DA5B23E1BC9C8A	FASTA	156	17,436
	10 20 30 40 50 60 MASPHQEPKP GDLIEIFRLG YEHWALYIGD GYVIHLAPPS EYPGAGSSSV FSVLSNSAEV 70 80 90 100 110 120 KRERLEDVVG GCCYRVNNSL DHEYQPRPVE VIISSAKEMV GQKMKYSIVS RNCEHFVTQL 130 140 150 RYGKSRCKQV RTSLDNPSPS AWGWTHGAVQ PGDHCE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a retinoid-induced class II tumor suppressor/growth regulatory gene." DiSepio D., Ghosn C., Eckert R.L., Deucher A., Robinson N., Duvic M., Chandraratna R.A.S., Nagpal S. Proc. Natl. Acad. Sci. U.S.A. 95:14811-14815(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Keratinocyte.
[2]	"Cloning and characterization of a novel retinoid-inducible gene 1(RIG1) deriving from human gastric cancer cells." Huang S.L., Shyu R.Y., Yeh M.Y., Jiang S.Y. Mol. Cell. Endocrinol. 159:15-24(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION. Tissue: Stomach cancer.
[3]	"Characterization of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes." Uyama T., Jin X.H., Tsuboi K., Tonai T., Ueda N. Biochim. Biophys. Acta 1791:1114-1124(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Testis.
[4]	Kato S. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Gastric adenocarcinoma.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Spleen and Urinary bladder.
[6]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF060228 mRNA. Translation: AAC84000.1.
AF092922 mRNA. Translation: AAF02294.1.
AB453252 mRNA. Translation: BAH22446.1.
AB030815 mRNA. Translation: BAB08109.1.
AK293357 mRNA. Translation: BAG56873.1.
AK312110 mRNA. Translation: BAG35046.1.
AP001591 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74156.1.
BC009678 mRNA. Translation: AAH09678.1.

IPI

IPI00296991.
IPI00909431.

RefSeq

NP_004576.2. NM_004585.3.

UniGene

Hs.17466.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LKT	NMR	-	A	1-125	[»]

ProteinModelPortal

Q9UL19.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000255688.

PTM databases

PhosphoSite

Q9UL19.

Polymorphism databases

DMDM

20140910.

Proteomic databases

PaxDb

Q9UL19.

PRIDE

Q9UL19.

Protocols and materials databases

DNASU

5920.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000255688; ENSP00000255688; ENSG00000133321.
ENST00000439013; ENSP00000402943; ENSG00000133321.

GeneID

5920.

KEGG

hsa:5920.

UCSC

uc001nxf.4. human.

Organism-specific databases

CTD

5920.

GeneCards

GC11P063304.

H-InvDB

HIX0128662.

HGNC

HGNC:9869. RARRES3.

HPA

HPA011219.

MIM

605092. gene.

neXtProt

NX_Q9UL19.

PharmGKB

PA34230.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG116857.

HOGENOM

HOG000293227.

HOVERGEN

HBG028837.

InParanoid

Q9UL19.

OMA

RCKQVEK.

OrthoDB

EOG4WSWBT.

PhylomeDB

Q9UL19.

Gene expression databases

ArrayExpress

Q9UL19.

Bgee

Q9UL19.

CleanEx

HS_RARRES3.

Genevestigator

Q9UL19.

GermOnline

ENSG00000133321. Homo sapiens.

Family and domain databases

InterPro

IPR007053. LRAT-like_dom.
[Graphical view]

Pfam

PF04970. LRAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

5920.

NextBio

23052.

SOURCE

Search...

Entry information

Entry name

HRSL4_HUMAN

Accession

Primary (citable) accession number: Q9UL19
Secondary accession number(s): B2R599 , B4DDW2, E7ENZ7, O95200

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.