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Protein

Procollagen C-endopeptidase enhancer 2

Gene

PCOLCE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.1 Publication

GO - Molecular functioni

  • collagen binding Source: MGI
  • heparin binding Source: MGI
  • peptidase activator activity Source: MGI

GO - Biological processi

  • positive regulation of peptidase activity Source: GOC
Complete GO annotation...

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-HSA-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen C-endopeptidase enhancer 2
Alternative name(s):
Procollagen COOH-terminal proteinase enhancer 2
Short name:
PCPE-2
Short name:
Procollagen C-proteinase enhancer 2
Gene namesi
Name:PCOLCE2
Synonyms:PCPE2
ORF Names:UNQ250/PRO287
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:8739. PCOLCE2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33084.

Polymorphism and mutation databases

BioMutaiPCOLCE2.
DMDMi67470587.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 415392Procollagen C-endopeptidase enhancer 2PRO_0000022020Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 59PROSITE-ProRule annotation
Disulfide bondi86 ↔ 107PROSITE-ProRule annotation
Disulfide bondi154 ↔ 181PROSITE-ProRule annotation
Disulfide bondi208 ↔ 231PROSITE-ProRule annotation
Disulfide bondi297 ↔ 364By similarity
Disulfide bondi301 ↔ 367By similarity
Disulfide bondi312 ↔ 415By similarity
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

O-glycosylated; contains sialic acid.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9UKZ9.
PaxDbiQ9UKZ9.
PRIDEiQ9UKZ9.

PTM databases

iPTMnetiQ9UKZ9.
PhosphoSiteiQ9UKZ9.

Expressioni

Tissue specificityi

Highly expressed in the heart, trabecular meshwork, pituitary gland, bladder, mammary gland, trachea and placenta and weakly expressed in the brain. Expressed in cartilage.3 Publications

Gene expression databases

BgeeiQ9UKZ9.
CleanExiHS_PCOLCE2.
ExpressionAtlasiQ9UKZ9. baseline and differential.
GenevisibleiQ9UKZ9. HS.

Organism-specific databases

HPAiHPA013203.

Interactioni

Subunit structurei

Interacts with heparin with high affinity, and type I or II collagen.1 Publication

GO - Molecular functioni

  • collagen binding Source: MGI

Protein-protein interaction databases

BioGridi117746. 10 interactions.
STRINGi9606.ENSP00000295992.

Structurei

3D structure databases

ProteinModelPortaliQ9UKZ9.
SMRiQ9UKZ9. Positions 33-266, 292-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 144112CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 268115CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini297 – 415119NTRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IEMJ. Eukaryota.
ENOG410Y9AW. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000059592.
HOVERGENiHBG053528.
InParanoidiQ9UKZ9.
OMAiIRRGLNY.
PhylomeDBiQ9UKZ9.
TreeFamiTF316506.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00042. CUB. 2 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50242. SSF50242. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UKZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGANAWAPL CLLLAAATQL SRQQSPERPV FTCGGILTGE SGFIGSEGFP
60 70 80 90 100
GVYPPNSKCT WKITVPEGKV VVLNFRFIDL ESDNLCRYDF VDVYNGHANG
110 120 130 140 150
QRIGRFCGTF RPGALVSSGN KMMVQMISDA NTAGNGFMAM FSAAEPNERG
160 170 180 190 200
DQYCGGLLDR PSGSFKTPNW PDRDYPAGVT CVWHIVAPKN QLIELKFEKF
210 220 230 240 250
DVERDNYCRY DYVAVFNGGE VNDARRIGKY CGDSPPAPIV SERNELLIQF
260 270 280 290 300
LSDLSLTADG FIGHYIFRPK KLPTTTEQPV TTTFPVTTGL KPTVALCQQK
310 320 330 340 350
CRRTGTLEGN YCSSDFVLAG TVITTITRDG SLHATVSIIN IYKEGNLAIQ
360 370 380 390 400
QAGKNMSARL TVVCKQCPLL RRGLNYIIMG QVGEDGRGKI MPNSFIMMFK
410
TKNQKLLDAL KNKQC
Length:415
Mass (Da):45,717
Last modified:May 1, 2000 - v1
Checksum:iB9AA87FD9AAF7A5B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti280 – 2801V → A.
Corresponds to variant rs35692900 [ dbSNP | Ensembl ].
VAR_051264
Natural varianti292 – 2921P → T.
Corresponds to variant rs17554211 [ dbSNP | Ensembl ].
VAR_022448

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098269 mRNA. Translation: AAF04621.1.
AY035400 mRNA. Translation: AAK63128.1.
AY358557 mRNA. Translation: AAQ88921.1.
CR536570 mRNA. Translation: CAG38807.1.
AK315121 mRNA. Translation: BAG37576.1.
CH471052 Genomic DNA. Translation: EAW78961.1.
CH471052 Genomic DNA. Translation: EAW78962.1.
CCDSiCCDS3127.1.
RefSeqiNP_037495.1. NM_013363.3.
UniGeneiHs.8944.

Genome annotation databases

EnsembliENST00000295992; ENSP00000295992; ENSG00000163710.
GeneIDi26577.
KEGGihsa:26577.
UCSCiuc003evd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098269 mRNA. Translation: AAF04621.1.
AY035400 mRNA. Translation: AAK63128.1.
AY358557 mRNA. Translation: AAQ88921.1.
CR536570 mRNA. Translation: CAG38807.1.
AK315121 mRNA. Translation: BAG37576.1.
CH471052 Genomic DNA. Translation: EAW78961.1.
CH471052 Genomic DNA. Translation: EAW78962.1.
CCDSiCCDS3127.1.
RefSeqiNP_037495.1. NM_013363.3.
UniGeneiHs.8944.

3D structure databases

ProteinModelPortaliQ9UKZ9.
SMRiQ9UKZ9. Positions 33-266, 292-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117746. 10 interactions.
STRINGi9606.ENSP00000295992.

PTM databases

iPTMnetiQ9UKZ9.
PhosphoSiteiQ9UKZ9.

Polymorphism and mutation databases

BioMutaiPCOLCE2.
DMDMi67470587.

Proteomic databases

MaxQBiQ9UKZ9.
PaxDbiQ9UKZ9.
PRIDEiQ9UKZ9.

Protocols and materials databases

DNASUi26577.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295992; ENSP00000295992; ENSG00000163710.
GeneIDi26577.
KEGGihsa:26577.
UCSCiuc003evd.4. human.

Organism-specific databases

CTDi26577.
GeneCardsiPCOLCE2.
HGNCiHGNC:8739. PCOLCE2.
HPAiHPA013203.
MIMi607064. gene.
neXtProtiNX_Q9UKZ9.
PharmGKBiPA33084.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEMJ. Eukaryota.
ENOG410Y9AW. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000059592.
HOVERGENiHBG053528.
InParanoidiQ9UKZ9.
OMAiIRRGLNY.
PhylomeDBiQ9UKZ9.
TreeFamiTF316506.

Enzyme and pathway databases

ReactomeiR-HSA-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

GeneWikiiPCOLCE2.
GenomeRNAii26577.
NextBioi48922.
PROiQ9UKZ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UKZ9.
CleanExiHS_PCOLCE2.
ExpressionAtlasiQ9UKZ9. baseline and differential.
GenevisibleiQ9UKZ9. HS.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00042. CUB. 2 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50242. SSF50242. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression of a novel type I procollagen C-proteinase enhancer protein gene from the glaucoma candidate region on 3q21-q24."
    Xu H., Acott T.S., Wirtz M.K.
    Genomics 66:264-273(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Identification and initial characterization of 5000 expressed sequenced tags (ESTs) each from adult human normal and osteoarthritic cartilage cDNA libraries."
    Kumar S., Connor J.R., Dodds R.A., Halsey W., Van Horn M., Mao J., Sathe G.M., Mui P., Agarwal P., Badger A.M., Lee J.C., Gowen M., Lark M.W.
    Osteoarthritis Cartilage 9:641-653(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], TISSUE SPECIFICITY.
    Tissue: Osteoarthritic cartilage.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Synovium.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-38.
  8. "PCOLCE2 encodes a functional procollagen C-proteinase enhancer (PCPE2) that is a collagen-binding protein differing in distribution of expression and post-translational modification from the previously described PCPE1."
    Steiglitz B.M., Keene D.R., Greenspan D.S.
    J. Biol. Chem. 277:49820-49830(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HEPARIN AND TYPE I OR II COLLAGEN, TISSUE SPECIFICITY, GLYCOSYLATION.

Entry informationi

Entry nameiPCOC2_HUMAN
AccessioniPrimary (citable) accession number: Q9UKZ9
Secondary accession number(s): B2RCH9, D3DNG4, Q9BRH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 2000
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.