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Q9UKY1

- ZHX1_HUMAN

UniProt

Q9UKY1 - ZHX1_HUMAN

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Protein

Zinc fingers and homeoboxes protein 1

Gene

ZHX1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Increases DNMT3B-mediated repressive transcriptional activity when DNMT3B is tethered to DNA. May link molecule between DNMT3B and other co-repressor proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9324C2H2-type 1CuratedPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri102 – 12524C2H2-type 2CuratedPROSITE-ProRule annotationAdd
BLAST
DNA bindingi284 – 34663Homeobox 1CuratedPROSITE-ProRule annotationAdd
BLAST
DNA bindingi464 – 52663Homeobox 2CuratedPROSITE-ProRule annotationAdd
BLAST
DNA bindingi569 – 63062Homeobox 3CuratedPROSITE-ProRule annotationAdd
BLAST
DNA bindingi660 – 72263Homeobox 4CuratedPROSITE-ProRule annotationAdd
BLAST
DNA bindingi777 – 83256Homeobox 5CuratedPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein heterodimerization activity Source: UniProtKB
  4. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  5. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. negative regulation of transcription, DNA-templated Source: UniProtKB
  3. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 1
Gene namesi
Name:ZHX1
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:12871. ZHX1.

Subcellular locationi

Nucleus 2 PublicationsPROSITE-ProRule annotation
Note: Colocalized in the nucleus with DNMT3B.

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37460.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Zinc fingers and homeoboxes protein 1PRO_0000049388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451Phosphoserine1 Publication
Modified residuei47 – 471Phosphoserine2 Publications
Modified residuei48 – 481Phosphoserine2 Publications
Modified residuei648 – 6481Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UKY1.
PaxDbiQ9UKY1.
PRIDEiQ9UKY1.

PTM databases

PhosphoSiteiQ9UKY1.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in podocytes.2 Publications

Gene expression databases

BgeeiQ9UKY1.
CleanExiHS_ZHX1.
GenevestigatoriQ9UKY1.

Organism-specific databases

HPAiHPA051900.
HPA055357.

Interactioni

Subunit structurei

Forms homodimers. Heterodimer (via HD1 domain) with ZHX2 (via HD1 domain). Also forms a heterodimer with ZHX3 which is a prerequisite for repressor activity. Interacts with ATF7IP and NFYA. Interacts (via homeobox domains) with DNMT3B (via PWWP domain).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATXN1P542535EBI-347767,EBI-930964
DNMT3BQ9UBC3-14EBI-347767,EBI-6083193

Protein-protein interaction databases

BioGridi116406. 57 interactions.
IntActiQ9UKY1. 56 interactions.
MINTiMINT-1424551.
STRINGi9606.ENSP00000297857.

Structurei

Secondary structure

1
873
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni60 – 623Combined sources
Beta strandi68 – 714Combined sources
Beta strandi78 – 803Combined sources
Helixi82 – 9211Combined sources
Beta strandi102 – 1043Combined sources
Turni105 – 1084Combined sources
Beta strandi109 – 1135Combined sources
Helixi115 – 1184Combined sources
Helixi120 – 1234Combined sources
Beta strandi131 – 1355Combined sources
Beta strandi144 – 1507Combined sources
Helixi473 – 48513Combined sources
Helixi491 – 50111Combined sources
Helixi505 – 51814Combined sources
Turni519 – 5235Combined sources
Helixi577 – 58913Combined sources
Helixi595 – 60410Combined sources
Helixi609 – 62416Combined sources
Beta strandi664 – 6674Combined sources
Helixi669 – 68113Combined sources
Helixi687 – 69711Combined sources
Helixi701 – 71515Combined sources
Turni716 – 7183Combined sources
Helixi721 – 73010Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ECBNMR-A565-640[»]
2GHFNMR-A60-153[»]
2LY9NMR-A462-532[»]
3NARX-ray2.60A/B655-731[»]
ProteinModelPortaliQ9UKY1.
SMRiQ9UKY1. Positions 60-153, 297-339, 462-532, 565-637, 662-731.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKY1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni272 – 564293Required for interaction with NFYAAdd
BLAST
Regioni272 – 432161Required for dimerizationAdd
BLAST
Regioni734 – 76835Required for nuclear localizationAdd
BLAST
Regioni831 – 87343Required for repressor activityAdd
BLAST

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.CuratedPROSITE-ProRule annotation
Contains 5 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9324C2H2-type 1CuratedPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri102 – 12524C2H2-type 2CuratedPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG39488.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ9UKY1.
OMAiSHMGYEQ.
OrthoDBiEOG76HQ0T.
PhylomeDBiQ9UKY1.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 5 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 4 hits.
[Graphical view]
SMARTiSM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 5 hits.
PROSITEiPS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UKY1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRRKSTTP CMVLASEQDP DLELISDLDE GPPVLTPVEN TRAESISSDE
60 70 80 90 100
EVHESVDSDN QQNKKVEGGY ECKYCTFQTP DLNMFTFHVD SEHPNVVLNS
110 120 130 140 150
SYVCVECNFL TKRYDALSEH NLKYHPGEEN FKLTMVKRNN QTIFEQTIND
160 170 180 190 200
LTFDGSFVKE ENAEQAESTE VSSSGISISK TPIMKMMKNK VENKRIAVHH
210 220 230 240 250
NSVEDVPEEK ENEIKPDREE IVENPSSSAS ESNTSTSIVN RIHPSTASTV
260 270 280 290 300
VTPAAVLPGL AQVITAVSAQ QNSNLIPKVL IPVNSIPTYN AALDNNPLLL
310 320 330 340 350
NTYNKFPYPT MSEITVLSAQ AKYTEEQIKI WFSAQRLKHG VSWTPEEVEE
360 370 380 390 400
ARRKQFNGTV HTVPQTITVI PTHISTGSNG LPSILQTCQI VGQPGLVLTQ
410 420 430 440 450
VAGTNTLPVT APIALTVAGV PSQNNIQKSQ VPAAQPTAET KPATAAVPTS
460 470 480 490 500
QSVKHETALV NPDSFGIRAK KTKEQLAELK VSYLKNQFPH DSEIIRLMKI
510 520 530 540 550
TGLTKGEIKK WFSDTRYNQR NSKSNQCLHL NNDSSTTIII DSSDETTESP
560 570 580 590 600
TVGTAQPKQS WNPFPDFTPQ KFKEKTAEQL RVLQASFLNS SVLTDEELNR
610 620 630 640 650
LRAQTKLTRR EIDAWFTEKK KSKALKEEKM EIDESNAGSS KEEAGETSPA
660 670 680 690 700
DESGAPKSGS TGKICKKTPE QLHMLKSAFV RTQWPSPEEY DKLAKESGLA
710 720 730 740 750
RTDIVSWFGD TRYAWKNGNL KWYYYYQSAN SSSMNGLSSL RKRGRGRPKG
760 770 780 790 800
RGRGRPRGRP RGSKRINNWD RGPSLIKFKT GTAILKDYYL KHKFLNEQDL
810 820 830 840 850
DELVNKSHMG YEQVREWFAE RQRRSELGIE LFEENEEEDE VIDDQEEDEE
860 870
ETDDSDTWEP PRHVKRKLSK SDD
Length:873
Mass (Da):98,098
Last modified:May 1, 2000 - v1
Checksum:i66CF1CEC5EF824E5
GO
Isoform 2 (identifier: Q96EF9-1) [UniParc]FASTAAdd to Basket

Also known as: ZHX1-C8orf76

The sequence of this isoform can be found in the external entry Q96EF9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Based on a readthrough transcript which may produce a ZHX1-C8orf76 fusion protein. No experimental confirmation available.

Length:292
Mass (Da):33,285
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631V → M in AAH40481. (PubMed:15489334)Curated
Sequence conflicti792 – 7921H → R in AAH40481. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF106862 mRNA. Translation: AAD50624.1.
AF195766 mRNA. Translation: AAF35183.1.
BC040481 mRNA. Translation: AAH40481.1.
CCDSiCCDS6342.1. [Q9UKY1-1]
PIRiJC7079.
RefSeqiNP_001017926.1. NM_001017926.2. [Q9UKY1-1]
NP_009153.3. NM_007222.4. [Q9UKY1-1]
UniGeneiHs.521800.

Genome annotation databases

GeneIDi11244.
KEGGihsa:11244.
UCSCiuc003yqe.3. human. [Q9UKY1-1]

Polymorphism databases

DMDMi44888551.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF106862 mRNA. Translation: AAD50624.1 .
AF195766 mRNA. Translation: AAF35183.1 .
BC040481 mRNA. Translation: AAH40481.1 .
CCDSi CCDS6342.1. [Q9UKY1-1 ]
PIRi JC7079.
RefSeqi NP_001017926.1. NM_001017926.2. [Q9UKY1-1 ]
NP_009153.3. NM_007222.4. [Q9UKY1-1 ]
UniGenei Hs.521800.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ECB NMR - A 565-640 [» ]
2GHF NMR - A 60-153 [» ]
2LY9 NMR - A 462-532 [» ]
3NAR X-ray 2.60 A/B 655-731 [» ]
ProteinModelPortali Q9UKY1.
SMRi Q9UKY1. Positions 60-153, 297-339, 462-532, 565-637, 662-731.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116406. 57 interactions.
IntActi Q9UKY1. 56 interactions.
MINTi MINT-1424551.
STRINGi 9606.ENSP00000297857.

PTM databases

PhosphoSitei Q9UKY1.

Polymorphism databases

DMDMi 44888551.

Proteomic databases

MaxQBi Q9UKY1.
PaxDbi Q9UKY1.
PRIDEi Q9UKY1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11244.
KEGGi hsa:11244.
UCSCi uc003yqe.3. human. [Q9UKY1-1 ]

Organism-specific databases

CTDi 11244.
GeneCardsi GC08M124260.
HGNCi HGNC:12871. ZHX1.
HPAi HPA051900.
HPA055357.
MIMi 604764. gene.
neXtProti NX_Q9UKY1.
PharmGKBi PA37460.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG39488.
GeneTreei ENSGT00530000063149.
HOGENOMi HOG000013231.
HOVERGENi HBG007920.
InParanoidi Q9UKY1.
OMAi SHMGYEQ.
OrthoDBi EOG76HQ0T.
PhylomeDBi Q9UKY1.
TreeFami TF333363.

Miscellaneous databases

EvolutionaryTracei Q9UKY1.
GeneWikii ZHX1.
GenomeRNAii 11244.
NextBioi 42786.
PROi Q9UKY1.
SOURCEi Search...

Gene expression databases

Bgeei Q9UKY1.
CleanExi HS_ZHX1.
Genevestigatori Q9UKY1.

Family and domain databases

Gene3Di 1.10.10.60. 5 hits.
InterProi IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF00046. Homeobox. 4 hits.
[Graphical view ]
SMARTi SM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 5 hits.
PROSITEi PS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y."
    Yamada K., Printz R.L., Osawa H., Granner D.K.
    Biochem. Biophys. Res. Commun. 261:614-621(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH NFYA.
    Tissue: Liver1 Publication.
  2. "Identification and cloning of the human ZHX1 gene."
    Mueller R., Ziegler B.L.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: TestisImported.
  4. "Identification of proteins that interact with NF-YA."
    Yamada K., Osawa H., Granner D.K.
    FEBS Lett. 460:41-45(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NFYA.
  5. Cited for: FUNCTION, HOMODIMERIZATION, SUBCELLULAR LOCATION.
  6. "Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3."
    Yamada K., Kawata H., Shou Z., Hirano S., Mizutani T., Yazawa T., Sekiguchi T., Yoshino M., Kajitani T., Miyamoto K.
    Biochem. J. 373:167-178(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: HETERODIMERIZATION WITH ZHX3, INTERACTION WITH ATF7IP.
  7. "Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor."
    Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K.
    Biochem. J. 373:747-757(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: HETERODIMERIZATION WITH ZHX2.
  8. "ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome."
    Liu G., Clement L.C., Kanwar Y.S., Avila-Casado C., Chugh S.S.
    J. Biol. Chem. 281:39681-39692(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Kidney.
  9. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Zinc-fingers and homeoboxes 1 (ZHX1) binds DNA methyltransferase (DNMT) 3B to enhance DNMT3B-mediated transcriptional repression."
    Kim S.H., Park J., Choi M.C., Kim H.P., Park J.H., Jung Y., Lee J.H., Oh D.Y., Im S.A., Bang Y.J., Kim T.Y.
    Biochem. Biophys. Res. Commun. 355:318-323(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DNMT3B.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-47 AND SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-48 AND SER-648, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  14. "Solution structure of the zinc-finger region of human zinc-fingers and homeoboxes 1 (ZHX1)."
    Structural proteomics in Europe (SPINE)
    Submitted (APR-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 60-153.
  15. "The solution structure of the third homeobox domain of human zinc fingers and homeoboxes protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (FEB-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 565-640.
  16. "The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension."
    Wienk H., Lammers I., Hotze A., Wu J., Wechselberger R.W., Owens R., Stammers D.K., Stuart D., Kaptein R., Folkers G.E.
    Biochemistry 48:4431-4439(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 60-153.
  17. "Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains."
    Bird L.E., Ren J., Nettleship J.E., Folkers G.E., Owens R.J., Stammers D.K.
    BMC Struct. Biol. 10:13-13(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 655-731.

Entry informationi

Entry nameiZHX1_HUMAN
AccessioniPrimary (citable) accession number: Q9UKY1
Secondary accession number(s): Q8IWD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3