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Protein

Nuclear pore complex protein Nup50

Gene

NUP50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.
SIGNORiQ9UKX7.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:NUP50
Synonyms:NPAP60L
ORF Names:PRO1146
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:8065. NUP50.

Subcellular locationi

  • Nucleusnuclear pore complex 1 Publication
  • Nucleus membrane By similarity; Peripheral membrane protein By similarity; Nucleoplasmic side By similarity

  • Note: Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity). Dissociates from the NPC structure early during prophase of mitosis. Associates to the newly formed nuclear membrane during telophase.By similarity

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nuclear membrane Source: UniProtKB
  • nuclear pore Source: ProtInc
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31852.

Polymorphism and mutation databases

BioMutaiNUP50.
DMDMi20455193.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Nuclear pore complex protein Nup50PRO_0000204868Add
BLAST
Isoform 2 (identifier: Q9UKX7-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N6-acetyllysineBy similarity
Modified residuei52 – 521PhosphoserineCombined sources
Modified residuei83 – 831N6-acetyllysineCombined sources
Modified residuei127 – 1271N6-acetyllysineBy similarity
Modified residuei208 – 2081PhosphoserineCombined sources
Modified residuei221 – 2211PhosphoserineCombined sources
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei246 – 2461PhosphothreonineCombined sources
Modified residuei259 – 2591PhosphothreonineCombined sources
Modified residuei270 – 2701PhosphoserineCombined sources
Modified residuei296 – 2961PhosphoserineCombined sources
Modified residuei450 – 4501N6-acetyllysineBy similarity
Isoform 2 (identifier: Q9UKX7-2)
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UKX7.
MaxQBiQ9UKX7.
PaxDbiQ9UKX7.
PeptideAtlasiQ9UKX7.
PRIDEiQ9UKX7.

PTM databases

iPTMnetiQ9UKX7.
PhosphoSiteiQ9UKX7.

Miscellaneous databases

PMAP-CutDBQ9UKX7.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

Gene expression databases

BgeeiENSG00000093000.
CleanExiHS_NUP50.
ExpressionAtlasiQ9UKX7. baseline and differential.
GenevisibleiQ9UKX7. HS.

Organism-specific databases

HPAiHPA047162.
HPA048328.

Interactioni

Subunit structurei

Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Does not interact with TPR.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
KPNA1P522944EBI-2371082,EBI-358383
KPNA2P522923EBI-2371082,EBI-349938
KPNA2Q6NVW73EBI-2371082,EBI-9377406
KPNA2Q7Z7263EBI-2371082,EBI-10323362
KPNA3Q8IYQ93EBI-2371082,EBI-742146
KPNA4O006293EBI-2371082,EBI-396343
KPNA5O151313EBI-2371082,EBI-540602
KPNA6O606843EBI-2371082,EBI-359923

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115982. 93 interactions.
IntActiQ9UKX7. 28 interactions.
MINTiMINT-3081797.
STRINGi9606.ENSP00000345895.

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni12 – 143Combined sources
Beta strandi15 – 173Combined sources
Helixi32 – 376Combined sources
Beta strandi358 – 36811Combined sources
Beta strandi370 – 38516Combined sources
Beta strandi387 – 40115Combined sources
Turni420 – 4223Combined sources
Beta strandi423 – 4286Combined sources
Beta strandi436 – 4416Combined sources
Beta strandi443 – 4486Combined sources
Helixi452 – 46716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EC1NMR-A351-468[»]
3TJ3X-ray2.70C/D1-109[»]
ProteinModelPortaliQ9UKX7.
SMRiQ9UKX7. Positions 1-47, 351-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKX7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati76 – 7721
Repeati113 – 11422
Repeati225 – 22623
Repeati273 – 27424
Repeati303 – 30425
Domaini335 – 468134RanBD1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 3042295 X 2 AA repeats of F-GAdd
BLAST
Regioni144 – 20663Binding to CDKN1BBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi55 – 9137Gly-richAdd
BLAST
Compositional biasi268 – 33063Ser-richAdd
BLAST

Domaini

Contains FG repeats.

Sequence similaritiesi

Contains 1 RanBD1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9UKX7.
KOiK14295.
OMAiKTEDTPD.
OrthoDBiEOG091G0HML.
PhylomeDBiQ9UKX7.
TreeFamiTF106504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR015007. NUP2/50/61.
IPR011993. PH_dom-like.
IPR000156. Ran_bind_dom.
[Graphical view]
PfamiPF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view]
SMARTiSM00160. RanBD. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50196. RANBD1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UKX7-1) [UniParc]FASTAAdd to basket
Also known as: Npap60L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF
60 70 80 90 100
ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF
110 120 130 140 150
ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA
160 170 180 190 200
CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ
210 220 230 240 250
HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK
260 270 280 290 300
MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS
310 320 330 340 350
SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT
360 370 380 390 400
EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN
410 420 430 440 450
LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK
460
TSEDADELHK ILLEKKDA
Length:468
Mass (Da):50,144
Last modified:May 2, 2002 - v2
Checksum:i1F2775AE9AC8FAC4
GO
Isoform 2 (identifier: Q9UKX7-2) [UniParc]FASTAAdd to basket
Also known as: Npap60S

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: Contrarily to Npap60L, Npap60S does not displaces NLSs, but stabilizes their binding to importin-alpha.Combined sources
Show »
Length:440
Mass (Da):46,863
Checksum:iC78B5EF4366680E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351T → S in AAD53401 (PubMed:10449902).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2828Missing in isoform 2. 1 PublicationVSP_040633Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA. Translation: AAD53401.1.
AF116624 mRNA. Translation: AAF71047.1.
CR456533 mRNA. Translation: CAG30419.1.
AK314454 mRNA. Translation: BAG37062.1.
AL008718, Z82243 Genomic DNA. Translation: CAI17944.1.
Z82243, AL008718 Genomic DNA. Translation: CAI17926.1.
Z82243 Genomic DNA. Translation: CAQ07372.1.
Z82243, AL008718 Genomic DNA. Translation: CAQ07374.1.
AL008718, Z82243 Genomic DNA. Translation: CAQ08670.1.
CH471138 Genomic DNA. Translation: EAW73370.1.
CH471138 Genomic DNA. Translation: EAW73372.1.
BC016055 mRNA. Translation: AAH16055.1.
BC028125 mRNA. Translation: AAH28125.1.
BC070133 mRNA. Translation: AAH70133.1.
AL389949 mRNA. Translation: CAB97527.1.
AL389950 mRNA. Translation: CAB97528.1.
CCDSiCCDS14062.1. [Q9UKX7-1]
CCDS14063.1. [Q9UKX7-2]
RefSeqiNP_009103.2. NM_007172.3. [Q9UKX7-1]
NP_705931.1. NM_153645.2. [Q9UKX7-2]
XP_005261369.1. XM_005261312.1. [Q9UKX7-2]
XP_005261371.1. XM_005261314.1. [Q9UKX7-2]
XP_006724166.1. XM_006724103.1. [Q9UKX7-1]
XP_006724167.1. XM_006724104.1. [Q9UKX7-2]
XP_011528135.1. XM_011529833.1. [Q9UKX7-1]
UniGeneiHs.475103.

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000. [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000. [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000. [Q9UKX7-2]
GeneIDi10762.
KEGGihsa:10762.
UCSCiuc003bfr.4. human. [Q9UKX7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA. Translation: AAD53401.1.
AF116624 mRNA. Translation: AAF71047.1.
CR456533 mRNA. Translation: CAG30419.1.
AK314454 mRNA. Translation: BAG37062.1.
AL008718, Z82243 Genomic DNA. Translation: CAI17944.1.
Z82243, AL008718 Genomic DNA. Translation: CAI17926.1.
Z82243 Genomic DNA. Translation: CAQ07372.1.
Z82243, AL008718 Genomic DNA. Translation: CAQ07374.1.
AL008718, Z82243 Genomic DNA. Translation: CAQ08670.1.
CH471138 Genomic DNA. Translation: EAW73370.1.
CH471138 Genomic DNA. Translation: EAW73372.1.
BC016055 mRNA. Translation: AAH16055.1.
BC028125 mRNA. Translation: AAH28125.1.
BC070133 mRNA. Translation: AAH70133.1.
AL389949 mRNA. Translation: CAB97527.1.
AL389950 mRNA. Translation: CAB97528.1.
CCDSiCCDS14062.1. [Q9UKX7-1]
CCDS14063.1. [Q9UKX7-2]
RefSeqiNP_009103.2. NM_007172.3. [Q9UKX7-1]
NP_705931.1. NM_153645.2. [Q9UKX7-2]
XP_005261369.1. XM_005261312.1. [Q9UKX7-2]
XP_005261371.1. XM_005261314.1. [Q9UKX7-2]
XP_006724166.1. XM_006724103.1. [Q9UKX7-1]
XP_006724167.1. XM_006724104.1. [Q9UKX7-2]
XP_011528135.1. XM_011529833.1. [Q9UKX7-1]
UniGeneiHs.475103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EC1NMR-A351-468[»]
3TJ3X-ray2.70C/D1-109[»]
ProteinModelPortaliQ9UKX7.
SMRiQ9UKX7. Positions 1-47, 351-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115982. 93 interactions.
IntActiQ9UKX7. 28 interactions.
MINTiMINT-3081797.
STRINGi9606.ENSP00000345895.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ9UKX7.
PhosphoSiteiQ9UKX7.

Polymorphism and mutation databases

BioMutaiNUP50.
DMDMi20455193.

Proteomic databases

EPDiQ9UKX7.
MaxQBiQ9UKX7.
PaxDbiQ9UKX7.
PeptideAtlasiQ9UKX7.
PRIDEiQ9UKX7.

Protocols and materials databases

DNASUi10762.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000. [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000. [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000. [Q9UKX7-2]
GeneIDi10762.
KEGGihsa:10762.
UCSCiuc003bfr.4. human. [Q9UKX7-1]

Organism-specific databases

CTDi10762.
GeneCardsiNUP50.
HGNCiHGNC:8065. NUP50.
HPAiHPA047162.
HPA048328.
MIMi604646. gene.
neXtProtiNX_Q9UKX7.
PharmGKBiPA31852.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9UKX7.
KOiK14295.
OMAiKTEDTPD.
OrthoDBiEOG091G0HML.
PhylomeDBiQ9UKX7.
TreeFamiTF106504.

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.
SIGNORiQ9UKX7.

Miscellaneous databases

EvolutionaryTraceiQ9UKX7.
GeneWikiiNUP50.
GenomeRNAii10762.
PMAP-CutDBQ9UKX7.
PROiQ9UKX7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000093000.
CleanExiHS_NUP50.
ExpressionAtlasiQ9UKX7. baseline and differential.
GenevisibleiQ9UKX7. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR015007. NUP2/50/61.
IPR011993. PH_dom-like.
IPR000156. Ran_bind_dom.
[Graphical view]
PfamiPF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view]
SMARTiSM00160. RanBD. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50196. RANBD1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP50_HUMAN
AccessioniPrimary (citable) accession number: Q9UKX7
Secondary accession number(s): B1AHA4
, B2RB15, O75644, Q8N6V5, Q9NPM9, Q9NPR6, Q9P1K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.