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Protein

Nuclear pore complex protein Nup50

Gene

NUP50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159227. Transport of the SLBP independent Mature mRNA.
R-HSA-159230. Transport of the SLBP Dependant Mature mRNA.
R-HSA-159231. Transport of Mature mRNA Derived from an Intronless Transcript.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168271. Transport of Ribonucleoproteins into the Host Nucleus.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-191859. snRNP Assembly.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4551638. SUMOylation of chromatin organization proteins.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.
SIGNORiQ9UKX7.

Protein family/group databases

TCDBi1.I.1.1.3. the eukaryotic nuclear pore complex (e-npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:NUP50
Synonyms:NPAP60L
ORF Names:PRO1146
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000093000.18.
HGNCiHGNC:8065. NUP50.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10762.
OpenTargetsiENSG00000093000.
PharmGKBiPA31852.

Polymorphism and mutation databases

BioMutaiNUP50.
DMDMi20455193.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048681 – 468Nuclear pore complex protein Nup50Add BLAST468
Isoform 2 (identifier: Q9UKX7-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei52PhosphoserineCombined sources1
Modified residuei83N6-acetyllysineCombined sources1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei208PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei234PhosphoserineBy similarity1
Modified residuei246PhosphothreonineCombined sources1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei296PhosphoserineCombined sources1
Cross-linki353Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei450N6-acetyllysineBy similarity1
Isoform 2 (identifier: Q9UKX7-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UKX7.
PaxDbiQ9UKX7.
PeptideAtlasiQ9UKX7.
PRIDEiQ9UKX7.

PTM databases

iPTMnetiQ9UKX7.
PhosphoSitePlusiQ9UKX7.

Miscellaneous databases

PMAP-CutDBiQ9UKX7.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

Gene expression databases

BgeeiENSG00000093000.
CleanExiHS_NUP50.
ExpressionAtlasiQ9UKX7. baseline and differential.
GenevisibleiQ9UKX7. HS.

Organism-specific databases

HPAiHPA047162.
HPA048328.

Interactioni

Subunit structurei

Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Does not interact with TPR.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115982. 101 interactors.
IntActiQ9UKX7. 28 interactors.
MINTiMINT-3081797.
STRINGi9606.ENSP00000345895.

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Beta strandi15 – 17Combined sources3
Helixi32 – 37Combined sources6
Beta strandi358 – 368Combined sources11
Beta strandi370 – 385Combined sources16
Beta strandi387 – 401Combined sources15
Turni420 – 422Combined sources3
Beta strandi423 – 428Combined sources6
Beta strandi436 – 441Combined sources6
Beta strandi443 – 448Combined sources6
Helixi452 – 467Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EC1NMR-A351-468[»]
3TJ3X-ray2.70C/D1-109[»]
ProteinModelPortaliQ9UKX7.
SMRiQ9UKX7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKX7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati113 – 11422
Repeati225 – 22632
Repeati273 – 27442
Repeati303 – 30452
Domaini335 – 468RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3045 X 2 AA repeats of F-GAdd BLAST229
Regioni144 – 206Binding to CDKN1BBy similarityAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 91Gly-richAdd BLAST37
Compositional biasi268 – 330Ser-richAdd BLAST63

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9UKX7.
KOiK14295.
OMAiTFLFHGN.
OrthoDBiEOG091G0HML.
PhylomeDBiQ9UKX7.
TreeFamiTF106504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR015007. NUP2/50/61.
IPR011993. PH-like_dom_sf.
IPR000156. Ran_bind_dom.
PfamiView protein in Pfam
PF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
SMARTiView protein in SMART
SM00160. RanBD. 1 hit.
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50196. RANBD1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UKX7-1) [UniParc]FASTAAdd to basket
Also known as: Npap60L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF
60 70 80 90 100
ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF
110 120 130 140 150
ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA
160 170 180 190 200
CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ
210 220 230 240 250
HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK
260 270 280 290 300
MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS
310 320 330 340 350
SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT
360 370 380 390 400
EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN
410 420 430 440 450
LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK
460
TSEDADELHK ILLEKKDA
Length:468
Mass (Da):50,144
Last modified:May 2, 2002 - v2
Checksum:i1F2775AE9AC8FAC4
GO
Isoform 2 (identifier: Q9UKX7-2) [UniParc]FASTAAdd to basket
Also known as: Npap60S

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: Contrarily to Npap60L, Npap60S does not displaces NLSs, but stabilizes their binding to importin-alpha.Combined sources
Show »
Length:440
Mass (Da):46,863
Checksum:iC78B5EF4366680E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235T → S in AAD53401 (PubMed:10449902).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0406331 – 28Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA. Translation: AAD53401.1.
AF116624 mRNA. Translation: AAF71047.1.
CR456533 mRNA. Translation: CAG30419.1.
AK314454 mRNA. Translation: BAG37062.1.
AL008718 Genomic DNA. No translation available.
Z82243 Genomic DNA. No translation available.
CH471138 Genomic DNA. Translation: EAW73370.1.
CH471138 Genomic DNA. Translation: EAW73372.1.
BC016055 mRNA. Translation: AAH16055.1.
BC028125 mRNA. Translation: AAH28125.1.
BC070133 mRNA. Translation: AAH70133.1.
AL389949 mRNA. Translation: CAB97527.1.
AL389950 mRNA. Translation: CAB97528.1.
CCDSiCCDS14062.1. [Q9UKX7-1]
CCDS14063.1. [Q9UKX7-2]
RefSeqiNP_009103.2. NM_007172.3. [Q9UKX7-1]
NP_705931.1. NM_153645.2. [Q9UKX7-2]
XP_005261369.1. XM_005261312.1. [Q9UKX7-2]
XP_005261371.1. XM_005261314.1. [Q9UKX7-2]
XP_006724166.1. XM_006724103.1. [Q9UKX7-1]
XP_006724167.1. XM_006724104.1. [Q9UKX7-2]
XP_011528135.1. XM_011529833.1. [Q9UKX7-1]
XP_016884026.1. XM_017028537.1. [Q9UKX7-2]
UniGeneiHs.475103.

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000. [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000. [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000. [Q9UKX7-2]
GeneIDi10762.
KEGGihsa:10762.
UCSCiuc003bfr.4. human. [Q9UKX7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUP50_HUMAN
AccessioniPrimary (citable) accession number: Q9UKX7
Secondary accession number(s): B1AHA4
, B2RB15, O75644, Q8N6V5, Q9NPM9, Q9NPR6, Q9P1K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: November 22, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references