Q9UKW6 (ELF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ETS-related transcription factor Elf-5

Alternative name(s):
E74-like factor 5
Epithelium-restricted ESE-1-related Ets factor
Epithelium-specific Ets transcription factor 2
Short name=ESE-2

Gene names

Name:	ELF5
Synonyms:	ESE2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Ref.2 Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters. Ref.2
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression. Ref.1 Ref.2
Domain	The PNT domain acts as a transcriptional activator By similarity.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Traceable author statement Ref.1. Source: ProtInc ectoderm development Inferred from electronic annotation. Source: Compara mammary gland epithelial cell differentiation Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UKW6-1)

Also known as: ESE-2a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UKW6-2)

Also known as: ESE-2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Isoform 3 (identifier: Q9UKW6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.
     130-148: YSFFNDAEESKATIKDYAD → QCSEGQTSRGGTRIRTKQL
     149-265: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 265

265

ETS-related transcription factor Elf-5

PRO_0000204092

Regions

Domain

43 – 129

87

PNT

DNA binding

173 – 254

82

ETS

Natural variations

Alternative sequence

1 – 10

10

Missing in isoform 2 and isoform 3.

VSP_014510

Alternative sequence

130 – 148

19

YSFFN…KDYAD → QCSEGQTSRGGTRIRTKQL in isoform 3.

VSP_014511

Alternative sequence

149 – 265

117

Missing in isoform 3.

VSP_014512

Experimental info

Sequence conflict

250

1

V → M in AAD22960. Ref.2

Sequence conflict

250

1

V → M in AAD22961. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

265

Helix Strand Turn

Details...

Helix

175 – 184

10

Turn

186 – 188

3

Beta strand

194 – 197

4

Turn

198 – 201

4

Beta strand

202 – 204

3

Helix

208 – 218

11

Helix

226 – 239

14

Beta strand

240 – 243

4

Beta strand

246 – 253

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ESE-2a) [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 43821A79A45768FE	FASTA	265	31,263
	10 20 30 40 50 60 MPSLPHSHRV MLDSVTHSTF LPNASFCDPL MSWTDLFSNE EYYPAFEHQT ACDSYWTSVH 70 80 90 100 110 120 PEYWTKRHVW EWLQFCCDQY KLDTNCISFC NFNISGLQLC SMTQEEFVEA AGLCGEYLYF 130 140 150 160 170 180 ILQNIRTQGY SFFNDAEESK ATIKDYADSN CLKTSGIKSQ DCHSHSRTSL QSSHLWEFVR 190 200 210 220 230 240 DLLLSPEENC GILEWEDREQ GIFRVVKSEA LAKMWGQRKK NDRMTYEKLS RALRYYYKTG 250 260 ILERVDRRLV YKFGKNAHGW QEDKL
	Isoform 2 (ESE-2b) [UniParc]. Checksum: AE61C5B178ECB555	FASTA	255	30,121
	10 20 30 40 50 60 MLDSVTHSTF LPNASFCDPL MSWTDLFSNE EYYPAFEHQT ACDSYWTSVH PEYWTKRHVW 70 80 90 100 110 120 EWLQFCCDQY KLDTNCISFC NFNISGLQLC SMTQEEFVEA AGLCGEYLYF ILQNIRTQGY 130 140 150 160 170 180 SFFNDAEESK ATIKDYADSN CLKTSGIKSQ DCHSHSRTSL QSSHLWEFVR DLLLSPEENC 190 200 210 220 230 240 GILEWEDREQ GIFRVVKSEA LAKMWGQRKK NDRMTYEKLS RALRYYYKTG ILERVDRRLV 250 YKFGKNAHGW QEDKL
	Isoform 3 [UniParc]. Checksum: CD6912714A9AF817	FASTA	138	16,121
	10 20 30 40 50 60 MLDSVTHSTF LPNASFCDPL MSWTDLFSNE EYYPAFEHQT ACDSYWTSVH PEYWTKRHVW 70 80 90 100 110 120 EWLQFCCDQY KLDTNCISFC NFNISGLQLC SMTQEEFVEA AGLCGEYLYF ILQNIRTQGQ 130 CSEGQTSRGG TRIRTKQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13-15, a region subject to LOH and rearrangement in human carcinoma cell lines." Zhou J., Ng A.Y., Tymms M.J., Jermiin L.S., Seth A.K., Thomas R.S., Kola I. Oncogene 17:2719-2732(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Lung.
[2]	"Characterization of ESE-2, a novel ESE-1-related Ets transcription factor that is restricted to glandular epithelium and differentiated keratinocytes." Oettgen P., Kas K., Dube A., Gu X., Grall F., Thamrongsak U., Akbarali Y., Finger E., Boltax J., Endress G., Munger K., Kunsch C., Libermann T.A. J. Biol. Chem. 274:29439-29452(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Kidney.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Urinary bladder.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF049703 mRNA. Translation: AAC79755.1.
AF115402 mRNA. Translation: AAD22960.1.
AF115403 mRNA. Translation: AAD22961.1.
AK074633 mRNA. Translation: BAC11101.1.
AK290817 mRNA. Translation: BAF83506.1.
AL137224 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68169.1.
BC029743 mRNA. Translation: AAH29743.1.

IPI

IPI00099434.
IPI00163115.
IPI00384023.

RefSeq

NP_001230009.1. NM_001243080.1.
NP_001230010.1. NM_001243081.1.
NP_001413.1. NM_001422.3.
NP_938195.1. NM_198381.1.

UniGene

Hs.11713.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WWX	NMR	-	A	172-265	[»]

ProteinModelPortal

Q9UKW6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UKW6. 5 interactions.

MINT

MINT-1448100.

STRING

9606.ENSP00000311010.

Polymorphism databases

DMDM

68565549.

Proteomic databases

PRIDE

Q9UKW6.

Protocols and materials databases

DNASU

2001.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000257832; ENSP00000257832; ENSG00000135374.
ENST00000312319; ENSP00000311010; ENSG00000135374.
ENST00000532417; ENSP00000436386; ENSG00000135374.

GeneID

2001.

KEGG

hsa:2001.

UCSC

uc001mvo.1. human.
uc001mvq.2. human.

Organism-specific databases

CTD

2001.

GeneCards

GC11M034500.

HGNC

HGNC:3320. ELF5.

MIM

605169. gene.

neXtProt

NX_Q9UKW6.

PharmGKB

PA27748.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG296414.

HOGENOM

HOG000232188.

HOVERGEN

HBG074143.

InParanoid

Q9UKW6.

KO

K09429.

OMA

SQGYSFF.

OrthoDB

EOG4V171B.

Gene expression databases

ArrayExpress

Q9UKW6.

Bgee

Q9UKW6.

CleanEx

HS_ELF5.

Genevestigator

Q9UKW6.

GermOnline

ENSG00000135374. Homo sapiens.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.
1.10.150.50. 1 hit.

InterPro

IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]

SUPFAM

SSF47769. SAM_homology. 1 hit.

PROSITE

PS00345. ETS_DOMAIN_1. False negative.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9UKW6.

GenomeRNAi

2001.

NextBio

8095.

SOURCE

Search...

Entry information

Entry name

ELF5_HUMAN

Accession

Primary (citable) accession number: Q9UKW6
Secondary accession number(s): A8K452 , O95175, Q8N2K9, Q96QY3, Q9UKW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.