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Protein

F-box/LRR-repeat protein 3

Gene

FBXL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed:17463251, PubMed:23452855, PubMed:27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed:17463251, PubMed:23452855, PubMed:27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed:23452855).3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Biological rhythms, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005812-MONOMER.
ReactomeiR-HSA-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-HSA-400253. Circadian Clock.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 3
Alternative name(s):
F-box and leucine-rich repeat protein 3A
F-box/LRR-repeat protein 3A
Gene namesi
Name:FBXL3
Synonyms:FBL3A, FBXL3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:13599. FBXL3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • nucleus Source: UniProtKB
  • SCF ubiquitin ligase complex Source: UniProtKB
  • ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi358C → A: Loss of binding with CRY1. 1 Publication1
Mutagenesisi358C → S: Decrease in binding efficiency with CRY2 and of CRY2 ubiquitination efficiency, loss of binding with CRY1. 1 Publication1

Organism-specific databases

DisGeNETi26224.
OpenTargetsiENSG00000005812.
PharmGKBiPA28022.

Polymorphism and mutation databases

BioMutaiFBXL3.
DMDMi37537866.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198421 – 428F-box/LRR-repeat protein 3Add BLAST428

Proteomic databases

EPDiQ9UKT7.
MaxQBiQ9UKT7.
PaxDbiQ9UKT7.
PeptideAtlasiQ9UKT7.
PRIDEiQ9UKT7.

PTM databases

iPTMnetiQ9UKT7.
PhosphoSitePlusiQ9UKT7.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000005812.
CleanExiHS_FBXL3.
ExpressionAtlasiQ9UKT7. baseline and differential.
GenevisibleiQ9UKT7. HS.

Organism-specific databases

HPAiHPA053283.
HPA065626.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXL3) composed of CUL1, SKP1, RBX1 and FBXL3. Interacts with CRY1 and CRY2 (phosphorylated).1 Publication

Protein-protein interaction databases

BioGridi117615. 15 interactors.
DIPiDIP-40770N.
IntActiQ9UKT7. 4 interactors.
MINTiMINT-108681.
STRINGi9606.ENSP00000347834.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi42 – 48Combined sources7
Helixi53 – 59Combined sources7
Helixi64 – 67Combined sources4
Turni68 – 71Combined sources4
Helixi73 – 76Combined sources4
Beta strandi77 – 83Combined sources7
Turni86 – 88Combined sources3
Beta strandi89 – 91Combined sources3
Helixi96 – 106Combined sources11
Turni107 – 109Combined sources3
Beta strandi112 – 117Combined sources6
Helixi121 – 131Combined sources11
Beta strandi140 – 149Combined sources10
Helixi151 – 154Combined sources4
Helixi157 – 170Combined sources14
Beta strandi176 – 178Combined sources3
Helixi186 – 196Combined sources11
Turni197 – 199Combined sources3
Beta strandi202 – 204Combined sources3
Helixi213 – 222Combined sources10
Beta strandi228 – 232Combined sources5
Helixi233 – 235Combined sources3
Helixi238 – 245Combined sources8
Beta strandi246 – 249Combined sources4
Beta strandi253 – 260Combined sources8
Helixi274 – 283Combined sources10
Beta strandi288 – 294Combined sources7
Beta strandi303 – 306Combined sources4
Beta strandi312 – 317Combined sources6
Helixi321 – 328Combined sources8
Beta strandi335 – 340Combined sources6
Helixi348 – 357Combined sources10
Beta strandi363 – 366Combined sources4
Helixi373 – 382Combined sources10
Helixi384 – 386Combined sources3
Beta strandi388 – 393Combined sources6
Helixi394 – 396Combined sources3
Helixi405 – 416Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6JX-ray2.70B1-428[»]
ProteinModelPortaliQ9UKT7.
SMRiQ9UKT7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 81F-boxAdd BLAST48
Repeati119 – 146LRR 1Add BLAST28
Repeati181 – 207LRR 2Add BLAST27
Repeati208 – 233LRR 3Add BLAST26
Repeati234 – 259LRR 4Add BLAST26
Repeati316 – 341LRR 5Add BLAST26
Repeati343 – 368LRR 6Add BLAST26
Repeati369 – 394LRR 7Add BLAST26

Sequence similaritiesi

Contains 1 F-box domain.Curated
Contains 7 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IT1T. Eukaryota.
ENOG410XT8H. LUCA.
GeneTreeiENSGT00390000007432.
HOGENOMiHOG000060185.
HOVERGENiHBG051587.
InParanoidiQ9UKT7.
KOiK10269.
OMAiCRSWNQV.
OrthoDBiEOG091G0R67.
PhylomeDBiQ9UKT7.
TreeFamiTF352583.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UKT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRGGRDSDR NSSEEGTAEK SKKLRTTNEH SQTCDWGNLL QDIILQVFKY
60 70 80 90 100
LPLLDRAHAS QVCRNWNQVF HMPDLWRCFE FELNQPATSY LKATHPELIK
110 120 130 140 150
QIIKRHSNHL QYVSFKVDSS KESAEAACDI LSQLVNCSLK TLGLISTARP
160 170 180 190 200
SFMDLPKSHF ISALTVVFVN SKSLSSLKID DTPVDDPSLK VLVANNSDTL
210 220 230 240 250
KLLKMSSCPH VSPAGILCVA DQCHGLRELA LNYHLLSDEL LLALSSEKHV
260 270 280 290 300
RLEHLRIDVV SENPGQTHFH TIQKSSWDAF IRHSPKVNLV MYFFLYEEEF
310 320 330 340 350
DPFFRYEIPA THLYFGRSVS KDVLGRVGMT CPRLVELVVC ANGLRPLDEE
360 370 380 390 400
LIRIAERCKN LSAIGLGECE VSCSAFVEFV KMCGGRLSQL SIMEEVLIPD
410 420
QKYSLEQIHW EVSKHLGRVW FPDMMPTW
Length:428
Mass (Da):48,707
Last modified:May 1, 2000 - v1
Checksum:iAAEAB31BAF0EA5A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10R → H in AAF37383 (Ref. 2) Curated1
Sequence conflicti13S → A in AAF37383 (Ref. 2) Curated1
Sequence conflicti353R → A in AAF37383 (Ref. 2) Curated1
Sequence conflicti364I → V in BAG37051 (PubMed:14702039).Curated1
Sequence conflicti412V → A in BAG37051 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129532 mRNA. Translation: AAF04466.1.
AF126028 mRNA. Translation: AAF37383.1.
AK314442 mRNA. Translation: BAG37051.1.
BC072448 mRNA. Translation: AAH72448.1.
CCDSiCCDS9457.1.
RefSeqiNP_036290.1. NM_012158.2.
XP_005266393.1. XM_005266336.1.
UniGeneiHs.508284.

Genome annotation databases

EnsembliENST00000355619; ENSP00000347834; ENSG00000005812.
GeneIDi26224.
KEGGihsa:26224.
UCSCiuc001vkd.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129532 mRNA. Translation: AAF04466.1.
AF126028 mRNA. Translation: AAF37383.1.
AK314442 mRNA. Translation: BAG37051.1.
BC072448 mRNA. Translation: AAH72448.1.
CCDSiCCDS9457.1.
RefSeqiNP_036290.1. NM_012158.2.
XP_005266393.1. XM_005266336.1.
UniGeneiHs.508284.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6JX-ray2.70B1-428[»]
ProteinModelPortaliQ9UKT7.
SMRiQ9UKT7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117615. 15 interactors.
DIPiDIP-40770N.
IntActiQ9UKT7. 4 interactors.
MINTiMINT-108681.
STRINGi9606.ENSP00000347834.

PTM databases

iPTMnetiQ9UKT7.
PhosphoSitePlusiQ9UKT7.

Polymorphism and mutation databases

BioMutaiFBXL3.
DMDMi37537866.

Proteomic databases

EPDiQ9UKT7.
MaxQBiQ9UKT7.
PaxDbiQ9UKT7.
PeptideAtlasiQ9UKT7.
PRIDEiQ9UKT7.

Protocols and materials databases

DNASUi26224.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355619; ENSP00000347834; ENSG00000005812.
GeneIDi26224.
KEGGihsa:26224.
UCSCiuc001vkd.4. human.

Organism-specific databases

CTDi26224.
DisGeNETi26224.
GeneCardsiFBXL3.
HGNCiHGNC:13599. FBXL3.
HPAiHPA053283.
HPA065626.
MIMi605653. gene.
neXtProtiNX_Q9UKT7.
OpenTargetsiENSG00000005812.
PharmGKBiPA28022.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IT1T. Eukaryota.
ENOG410XT8H. LUCA.
GeneTreeiENSGT00390000007432.
HOGENOMiHOG000060185.
HOVERGENiHBG051587.
InParanoidiQ9UKT7.
KOiK10269.
OMAiCRSWNQV.
OrthoDBiEOG091G0R67.
PhylomeDBiQ9UKT7.
TreeFamiTF352583.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000005812-MONOMER.
ReactomeiR-HSA-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-HSA-400253. Circadian Clock.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFBXL3. human.
GeneWikiiFBXL3.
GenomeRNAii26224.
PROiQ9UKT7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005812.
CleanExiHS_FBXL3.
ExpressionAtlasiQ9UKT7. baseline and differential.
GenevisibleiQ9UKT7. HS.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFBXL3_HUMAN
AccessioniPrimary (citable) accession number: Q9UKT7
Secondary accession number(s): B2RB04, Q9P122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.