ID   KLK12_HUMAN             Reviewed;         248 AA.
AC   Q9UKR0; Q9UKR1;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-JAN-2012, entry version 97.
DE   RecName: Full=Kallikrein-12;
DE            EC=3.4.21.-;
DE   AltName: Full=Kallikrein-like protein 5;
DE            Short=KLK-L5;
DE   Flags: Precursor;
GN   Name=KLK12; Synonyms=KLKL5; ORFNames=UNQ669/PRO1303;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE (ISOFORM 1).
RX   MEDLINE=20118156; PubMed=10652563;
RA   Yousef G.M., Luo L.-Y., Diamandis E.P.;
RT   "Identification of novel human kallikrein-like genes on chromosome
RT   19q13.3-q13.4.";
RL   Anticancer Res. 19:2843-2852(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
RA   Yousef G.M., Magklara A., Scorilas A., Diamandis E.P.;
RT   "Cloning of new alternatively spliced forms of the kallikrein-like
RT   gene 5 (KLK-L5).";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11054574; DOI=10.1016/S0378-1119(00)00382-6;
RA   Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J.,
RA   Moss P., Paeper B., Wang K.;
RT   "Sequencing and expression analysis of the serine protease gene
RT   cluster located in chromosome 19q13 region.";
RL   Gene 257:119-130(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   MEDLINE=22887296; PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA   Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA   Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA   Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA   Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA   Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA   Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale
RT   effort to identify novel human secreted and transmembrane proteins: a
RT   bioinformatics assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J.,
RA   Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M.,
RA   Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E.,
RA   Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M.,
RA   Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C.,
RA   Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M.,
RA   Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H.,
RA   Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S.,
RA   Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J.,
RA   Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M.,
RA   Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J.,
RA   Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D.,
RA   Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A.,
RA   Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I.,
RA   Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [6]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-24, AND MASS SPECTROMETRY.
RC   TISSUE=Milk;
RX   PubMed=18780401; DOI=10.1002/pmic.200701057;
RA   Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.;
RT   "Identification of N-linked glycoproteins in human milk by hydrophilic
RT   interaction liquid chromatography and mass spectrometry.";
RL   Proteomics 8:3833-3847(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9UKR0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9UKR0-2; Sequence=VSP_005403;
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 peptidase S1 domain.
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DR   EMBL; AF135025; AAD26426.2; -; Genomic_DNA.
DR   EMBL; AF135025; AAF06065.1; -; Genomic_DNA.
DR   EMBL; AF243527; AAG33365.1; -; Genomic_DNA.
DR   EMBL; AY358524; AAQ88888.1; -; mRNA.
DR   EMBL; AC011473; AAG23258.1; -; Genomic_DNA.
DR   IPI; IPI00007724; -.
DR   IPI; IPI00220155; -.
DR   RefSeq; NP_062544.1; NM_019598.2.
DR   RefSeq; NP_665901.1; NM_145894.1.
DR   UniGene; Hs.411572; -.
DR   ProteinModelPortal; Q9UKR0; -.
DR   SMR; Q9UKR0; 22-246.
DR   STRING; Q9UKR0; -.
DR   MEROPS; S01.020; -.
DR   DMDM; 9296989; -.
DR   PRIDE; Q9UKR0; -.
DR   Ensembl; ENST00000319590; ENSP00000324181; ENSG00000186474.
DR   GeneID; 43849; -.
DR   KEGG; hsa:43849; -.
DR   UCSC; uc002pvg.1; human.
DR   UCSC; uc002pvh.1; human.
DR   CTD; 43849; -.
DR   GeneCards; GC19M051532; -.
DR   H-InvDB; HIX0027516; -.
DR   HGNC; HGNC:6360; KLK12.
DR   HPA; CAB025473; -.
DR   MIM; 605539; gene.
DR   neXtProt; NX_Q9UKR0; -.
DR   PharmGKB; PA30149; -.
DR   GeneTree; ENSGT00590000082811; -.
DR   HOVERGEN; HBG013304; -.
DR   OMA; HPWNPFP; -.
DR   NextBio; 53004; -.
DR   ArrayExpress; Q9UKR0; -.
DR   Bgee; Q9UKR0; -.
DR   CleanEx; HS_KLK12; -.
DR   Genevestigator; Q9UKR0; -.
DR   GermOnline; ENSG00000186474; Homo sapiens.
DR   GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR   GO; GO:0005625; C:soluble fraction; IDA:UniProtKB.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; NAS:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; NAS:UniProtKB.
DR   InterPro; IPR009003; Pept_cys/ser_Trypsin-like.
DR   InterPro; IPR018114; Peptidase_S1/S6_AS.
DR   InterPro; IPR001254; Peptidase_S1_S6.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   KO; K09621; -.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; Pept_Ser_Cys; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Disulfide bond; Glycoprotein;
KW   Hydrolase; Protease; Reference proteome; Secreted; Serine protease;
KW   Signal.
FT   SIGNAL        1     17       Potential.
FT   CHAIN        18    248       Kallikrein-12.
FT                                /FTId=PRO_0000027956.
FT   DOMAIN       22    246       Peptidase S1.
FT   ACT_SITE     62     62       Charge relay system (By similarity).
FT   ACT_SITE    108    108       Charge relay system (By similarity).
FT   ACT_SITE    200    200       Charge relay system (By similarity).
FT   CARBOHYD     24     24       N-linked (GlcNAc...).
FT   CARBOHYD    163    163       N-linked (GlcNAc...) (Potential).
FT   DISULFID     28    161       By similarity.
FT   DISULFID     47     63       By similarity.
FT   DISULFID    133    235       By similarity.
FT   DISULFID    140    206       By similarity.
FT   DISULFID    172    186       By similarity.
FT   DISULFID    196    222       By similarity.
FT   VAR_SEQ     236    248       KYVDWIRMIMRNN -> NSTLVGLGTSWNFNSCQPF (in
FT                                isoform 2).
FT                                /FTId=VSP_005403.
SQ   SEQUENCE   248 AA;  26734 MW;  BB473E98F8BAF703 CRC64;
     MGLSIFLLLC VLGLSQAATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA
     AHCSGSRYWV RLGEHSLSQL DWTEQIRHSG FSVTHPGYLG ASTSHEHDLR LLRLRLPVRV
     TSSVQPLPLP NDCATAGTEC HVSGWGITNH PRNPFPDLLQ CLNLSIVSHA TCHGVYPGRI
     TSNMVCAGGV PGQDACQGDS GGPLVCGGVL QGLVSWGSVG PCGQDGIPGV YTYICKYVDW
     IRMIMRNN
//