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Q9UKR0 (KLK12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-12

EC=3.4.21.-
Alternative name(s):
Kallikrein-like protein 5
Short name=KLK-L5
Gene names
Name:KLK12
Synonyms:KLKL5
ORF Names:UNQ669/PRO1303
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from direct assay PubMed 21628462. Source: UniProtKB

   Cellular_componentextracellular space

Non-traceable author statement PubMed 21628462. Source: UniProtKB

   Molecular_functionpeptidase activity

Inferred from direct assay PubMed 21628462. Source: UniProtKB

serine-type endopeptidase activity

Non-traceable author statement Ref.4. Source: UniProtKB

serine-type peptidase activity

Inferred from direct assay PubMed 15300858. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UKR0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UKR0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-248: KYVDWIRMIMRNN → NSTLVGLGTSWNFNSCQPF
Isoform 3 (identifier: Q9UKR0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     66-111: SRYWVRLGEH...STSHEHDLRL → RPIPGSAPVP...RRPGAGCLPG
     112-248: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 248231Kallikrein-12
PRO_0000027956

Regions

Domain22 – 246225Peptidase S1

Sites

Active site621Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2001Charge relay system By similarity

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Ref.8
Glycosylation1631N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 161 By similarity
Disulfide bond47 ↔ 63 By similarity
Disulfide bond133 ↔ 235 By similarity
Disulfide bond140 ↔ 206 By similarity
Disulfide bond172 ↔ 186 By similarity
Disulfide bond196 ↔ 222 By similarity

Natural variations

Alternative sequence66 – 11146SRYWV…HDLRL → RPIPGSAPVPQPLHRLPCHL PWCVSRENHEQHGVCRRRPG AGCLPG in isoform 3.
VSP_045851
Alternative sequence112 – 248137Missing in isoform 3.
VSP_045852
Alternative sequence236 – 24813KYVDW…IMRNN → NSTLVGLGTSWNFNSCQPF in isoform 2.
VSP_005403

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: BB473E98F8BAF703

FASTA24826,734
        10         20         30         40         50         60 
MGLSIFLLLC VLGLSQAATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA 

        70         80         90        100        110        120 
AHCSGSRYWV RLGEHSLSQL DWTEQIRHSG FSVTHPGYLG ASTSHEHDLR LLRLRLPVRV 

       130        140        150        160        170        180 
TSSVQPLPLP NDCATAGTEC HVSGWGITNH PRNPFPDLLQ CLNLSIVSHA TCHGVYPGRI 

       190        200        210        220        230        240 
TSNMVCAGGV PGQDACQGDS GGPLVCGGVL QGLVSWGSVG PCGQDGIPGV YTYICKYVDW 


IRMIMRNN 

« Hide

Isoform 2 [UniParc].

Checksum: 704A1552D34DF188
Show »

FASTA25427,067
Isoform 3 [UniParc].

Checksum: 3E8EAB4295635D8D
Show »

FASTA11111,984

References

« Hide 'large scale' references
[1]"Identification of novel human kallikrein-like genes on chromosome 19q13.3-q13.4."
Yousef G.M., Luo L.-Y., Diamandis E.P.
Anticancer Res. 19:2843-2852(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]"Cloning of new alternatively spliced forms of the kallikrein-like gene 5 (KLK-L5)."
Yousef G.M., Magklara A., Scorilas A., Diamandis E.P.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[3]"The new human kallikrein gene family: implications in carcinogenesis."
Diamandis E.P., Yousef G.M., Luo L.Y., Magklara A., Obiezu C.V.
Trends Endocrinol. Metab. 11:54-60(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[4]"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region."
Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K.
Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.
Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-24.
Tissue: Milk.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF135025 Genomic DNA. Translation: AAD26426.2.
AF135025 Genomic DNA. Translation: AAF06065.1.
AF135025 Genomic DNA. Translation: AAF06066.1.
AF243527 Genomic DNA. Translation: AAG33365.1.
AY358524 mRNA. Translation: AAQ88888.1.
AC011473 Genomic DNA. Translation: AAG23258.1.
CH471135 Genomic DNA. Translation: EAW71976.1.
CCDSCCDS12820.1. [Q9UKR0-2]
CCDS12821.1. [Q9UKR0-1]
CCDS54298.1. [Q9UKR0-3]
RefSeqNP_062544.1. NM_019598.2. [Q9UKR0-2]
NP_665901.1. NM_145894.1. [Q9UKR0-1]
NP_665902.1. NM_145895.1. [Q9UKR0-3]
XP_005259008.1. XM_005258951.1. [Q9UKR0-3]
UniGeneHs.411572.

3D structure databases

ProteinModelPortalQ9UKR0.
SMRQ9UKR0. Positions 22-248.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9UKR0.
ChEMBLCHEMBL4943.

Protein family/group databases

MEROPSS01.020.

PTM databases

PhosphoSiteQ9UKR0.

Polymorphism databases

DMDM9296989.

Proteomic databases

PaxDbQ9UKR0.
PRIDEQ9UKR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000250351; ENSP00000250351; ENSG00000186474. [Q9UKR0-2]
ENST00000319590; ENSP00000324181; ENSG00000186474. [Q9UKR0-1]
ENST00000525263; ENSP00000436458; ENSG00000186474. [Q9UKR0-1]
ENST00000529888; ENSP00000434036; ENSG00000186474. [Q9UKR0-3]
GeneID43849.
KEGGhsa:43849.
UCSCuc002pvg.1. human. [Q9UKR0-1]
uc002pvh.1. human. [Q9UKR0-2]
uc002pvj.1. human.

Organism-specific databases

CTD43849.
GeneCardsGC19M051532.
HGNCHGNC:6360. KLK12.
HPACAB025473.
MIM605539. gene.
neXtProtNX_Q9UKR0.
PharmGKBPA30149.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
KOK09621.
OMAVPGQDAC.
OrthoDBEOG75B84T.
PhylomeDBQ9UKR0.
TreeFamTF331065.

Gene expression databases

ArrayExpressQ9UKR0.
BgeeQ9UKR0.
CleanExHS_KLK12.
GenevestigatorQ9UKR0.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLK12.
GenomeRNAi43849.
NextBio53004.
PROQ9UKR0.
SOURCESearch...

Entry information

Entry nameKLK12_HUMAN
AccessionPrimary (citable) accession number: Q9UKR0
Secondary accession number(s): Q9UKR1, Q9UKR2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM