Q9UKR0 (KLK12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-12
EC=3.4.21.-

Alternative name(s):
Kallikrein-like protein 5
Short name=KLK-L5

Gene names

Name:	KLK12
Synonyms:	KLKL5
ORF Names:	UNQ669/PRO1303

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from direct assay PubMed 21628462. Source: UniProtKB
Cellular_component	extracellular space Non-traceable author statement PubMed 21628462. Source: UniProtKB
Molecular_function	serine-type endopeptidase activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UKR0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UKR0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-248: KYVDWIRMIMRNN → NSTLVGLGTSWNFNSCQPF

Isoform 3 (identifier: Q9UKR0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     66-111: SRYWVRLGEH...STSHEHDLRL → RPIPGSAPVP...RRPGAGCLPG
     112-248: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 248	231	Kallikrein-12		PRO_0000027956
Regions
	Domain	22 – 246	225	Peptidase S1
Sites
	Active site	62	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Disulfide bond	28 ↔ 161		By similarity
	Disulfide bond	47 ↔ 63		By similarity
	Disulfide bond	133 ↔ 235		By similarity
	Disulfide bond	140 ↔ 206		By similarity
	Disulfide bond	172 ↔ 186		By similarity
	Disulfide bond	196 ↔ 222		By similarity
Natural variations
	Alternative sequence	66 – 111	46	SRYWV…HDLRL → RPIPGSAPVPQPLHRLPCHL PWCVSRENHEQHGVCRRRPG AGCLPG in isoform 3.		VSP_045851
	Alternative sequence	112 – 248	137	Missing in isoform 3.		VSP_045852
	Alternative sequence	236 – 248	13	KYVDW…IMRNN → NSTLVGLGTSWNFNSCQPF in isoform 2.		VSP_005403

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BB473E98F8BAF703	FASTA	248	26,734
	10 20 30 40 50 60 MGLSIFLLLC VLGLSQAATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA 70 80 90 100 110 120 AHCSGSRYWV RLGEHSLSQL DWTEQIRHSG FSVTHPGYLG ASTSHEHDLR LLRLRLPVRV 130 140 150 160 170 180 TSSVQPLPLP NDCATAGTEC HVSGWGITNH PRNPFPDLLQ CLNLSIVSHA TCHGVYPGRI 190 200 210 220 230 240 TSNMVCAGGV PGQDACQGDS GGPLVCGGVL QGLVSWGSVG PCGQDGIPGV YTYICKYVDW IRMIMRNN
	Isoform 2 [UniParc]. Checksum: 704A1552D34DF188	FASTA	254	27,067
	10 20 30 40 50 60 MGLSIFLLLC VLGLSQAATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA 70 80 90 100 110 120 AHCSGSRYWV RLGEHSLSQL DWTEQIRHSG FSVTHPGYLG ASTSHEHDLR LLRLRLPVRV 130 140 150 160 170 180 TSSVQPLPLP NDCATAGTEC HVSGWGITNH PRNPFPDLLQ CLNLSIVSHA TCHGVYPGRI 190 200 210 220 230 240 TSNMVCAGGV PGQDACQGDS GGPLVCGGVL QGLVSWGSVG PCGQDGIPGV YTYICNSTLV 250 GLGTSWNFNS CQPF
	Isoform 3 [UniParc]. Checksum: 3E8EAB4295635D8D	FASTA	111	11,984
	10 20 30 40 50 60 MGLSIFLLLC VLGLSQAATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA 70 80 90 100 110 AHCSGRPIPG SAPVPQPLHR LPCHLPWCVS RENHEQHGVC RRRPGAGCLP G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human kallikrein-like genes on chromosome 19q13.3-q13.4." Yousef G.M., Luo L.-Y., Diamandis E.P. Anticancer Res. 19:2843-2852(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]	"Cloning of new alternatively spliced forms of the kallikrein-like gene 5 (KLK-L5)." Yousef G.M., Magklara A., Scorilas A., Diamandis E.P. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[3]	"The new human kallikrein gene family: implications in carcinogenesis." Diamandis E.P., Yousef G.M., Luo L.Y., Magklara A., Obiezu C.V. Trends Endocrinol. Metab. 11:54-60(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[4]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F. Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-24, MASS SPECTROMETRY. Tissue: Milk.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135025 Genomic DNA. Translation: AAD26426.2. AF135025 Genomic DNA. Translation: AAF06065.1. AF135025 Genomic DNA. Translation: AAF06066.1. AF243527 Genomic DNA. Translation: AAG33365.1. AY358524 mRNA. Translation: AAQ88888.1. AC011473 Genomic DNA. Translation: AAG23258.1. CH471135 Genomic DNA. Translation: EAW71976.1.
IPI	IPI00007724. IPI00010516. IPI00220155.
RefSeq	NP_062544.1. NM_019598.2. NP_665901.1. NM_145894.1. NP_665902.1. NM_145895.1.
UniGene	Hs.411572.
3D structure databases
ProteinModelPortal	Q9UKR0.
ModBase	Search...
Protein family/group databases
MEROPS	S01.020.
PTM databases
PhosphoSite	Q9UKR0.
Polymorphism databases
DMDM	9296989.
Proteomic databases
PaxDb	Q9UKR0.
PRIDE	Q9UKR0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250351; ENSP00000250351; ENSG00000186474. ENST00000319590; ENSP00000324181; ENSG00000186474. ENST00000525263; ENSP00000436458; ENSG00000186474. ENST00000529888; ENSP00000434036; ENSG00000186474.
GeneID	43849.
KEGG	hsa:43849.
UCSC	uc002pvg.1. human. uc002pvh.1. human.
Organism-specific databases
CTD	43849.
GeneCards	GC19M051532.
HGNC	HGNC:6360. KLK12.
HPA	CAB025473.
MIM	605539. gene.
neXtProt	NX_Q9UKR0.
PharmGKB	PA30149.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
KO	K09621.
OMA	HPWNPFP.
Gene expression databases
ArrayExpress	Q9UKR0.
Bgee	Q9UKR0.
CleanEx	HS_KLK12.
Genevestigator	Q9UKR0.
GermOnline	ENSG00000186474. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9UKR0.
ChEMBL	CHEMBL4943.
GenomeRNAi	43849.
NextBio	53004.
SOURCE	Search...

Entry information

Entry name

KLK12_HUMAN

Accession

Primary (citable) accession number: Q9UKR0
Secondary accession number(s): Q9UKR1, Q9UKR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.