##gff-version 3 Q9UKM7 UniProtKB Chain 1 699 . . . ID=PRO_0000210314;Note=Endoplasmic reticulum mannosyl-oligosaccharide 1%2C2-alpha-mannosidase Q9UKM7 UniProtKB Topological domain 1 84 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9UKM7 UniProtKB Transmembrane 85 105 . . . Note=Helical%3B Signal-anchor for type II membrane protein;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9UKM7 UniProtKB Topological domain 106 699 . . . Note=Lumenal;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9UKM7 UniProtKB Region 125 243 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9UKM7 UniProtKB Compositional bias 147 162 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9UKM7 UniProtKB Compositional bias 177 197 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9UKM7 UniProtKB Active site 330 330 . . . Note=Proton donor;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9UKM7 UniProtKB Active site 463 463 . . . Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9UKM7 UniProtKB Active site 570 570 . . . Note=Proton donor;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P31723 Q9UKM7 UniProtKB Active site 599 599 . . . Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9UKM7 UniProtKB Binding site 688 688 . . . Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P32906 Q9UKM7 UniProtKB Disulfide bond 527 556 . . . Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10995765;Dbxref=PMID:10995765 Q9UKM7 UniProtKB Natural variant 59 59 . . . ID=VAR_055841;Note=N->S;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10409699,ECO:0000269|PubMed:10521544,ECO:0000269|PubMed:12975309,ECO:0000269|PubMed:15489334;Dbxref=dbSNP:rs968733,PMID:10409699,PMID:10521544,PMID:12975309,PMID:15489334 Q9UKM7 UniProtKB Natural variant 334 334 . . . ID=VAR_066592;Note=In RAFQS%3B results in about 1300-fold decrease in activity. R->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21763484;Dbxref=dbSNP:rs387906886,PMID:21763484 Q9UKM7 UniProtKB Natural variant 397 397 . . . ID=VAR_066593;Note=In RAFQS%3B disrupts stable protein expression. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21763484;Dbxref=dbSNP:rs387906885,PMID:21763484 Q9UKM7 UniProtKB Mutagenesis 330 330 . . . Note=About 44-fold reduction in K(cat)%2C slight reduction in K(m)%2C about 100-fold increase in binding affinity for Man(9)GlcnAc(2) but no change in binding affinity for the inhibitor%2C dMNJ. Even further greater reduction in K(cat) and increase in K(m)%3B when associated with Q-599. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15713668;Dbxref=PMID:15713668 Q9UKM7 UniProtKB Mutagenesis 463 463 . . . Note=Some reduction in K(cat) but no change in K(m)%2C abolishes almost all binding to Man(9)GlcnAc(2) but reduced binding to the inhibitor dMNJ by about 73-fold. Further reduction in K(m) but slight increase in K(m)%3B when associated with Q-599. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15713668;Dbxref=PMID:15713668 Q9UKM7 UniProtKB Mutagenesis 524 524 . . . Note=About 4-fold reduction in K(cat). H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15713668;Dbxref=PMID:15713668 Q9UKM7 UniProtKB Mutagenesis 599 599 . . . Note=Very significant reduction in K(cat)%2C 4-fold weaker binding affinity for Man(9)GlcnAc(2) but about 1000-fold reduction in binding affinity for the inhibitor%2C dMNJ. Significant reductions in K(cat) and slight increase in K(m)%3B when associated with E-330 or N-463. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15713668;Dbxref=PMID:15713668 Q9UKM7 UniProtKB Sequence conflict 204 204 . . . Note=T->A;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9UKM7 UniProtKB Sequence conflict 223 223 . . . Note=S->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9UKM7 UniProtKB Beta strand 244 246 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1FO3 Q9UKM7 UniProtKB Helix 248 267 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 271 275 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 276 279 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 280 282 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 284 287 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 289 300 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 304 317 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 325 327 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 328 346 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 349 362 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 363 366 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 368 370 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1FO3 Q9UKM7 UniProtKB Beta strand 375 378 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 379 381 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 391 394 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 395 399 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 402 412 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 416 429 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 441 444 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 445 447 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 450 452 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 460 462 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 463 475 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 481 497 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 499 501 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 503 505 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 508 510 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 512 514 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 517 519 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 521 523 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 524 527 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 528 538 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 543 561 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 563 566 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1FO2 Q9UKM7 UniProtKB Beta strand 570 573 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 584 586 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5KIJ Q9UKM7 UniProtKB Helix 589 591 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 599 611 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 615 630 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 659 662 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Helix 664 672 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Turn 676 679 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5KIJ Q9UKM7 UniProtKB Turn 681 683 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 684 686 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D Q9UKM7 UniProtKB Beta strand 692 694 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1X9D