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Q9UKK6

- NXT1_HUMAN

UniProt

Q9UKK6 - NXT1_HUMAN

Protein

NTF2-related export protein 1

Gene

NXT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. protein export from nucleus Source: Ensembl
    2. RNA export from nucleus Source: Ensembl

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NTF2-related export protein 1
    Alternative name(s):
    Protein p15
    Gene namesi
    Name:NXT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:15913. NXT1.

    Subcellular locationi

    Nucleus. Nucleus speckle Curated. Cytoplasm
    Note: Shuttles between the nucleus and the cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. nuclear pore Source: ProtInc
    3. nuclear speck Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi114 – 1141I → R: 70% reduction in mRNA export activity. 1 Publication
    Mutagenesisi134 – 1341R → D: 57% reduction in mRNA export activity and loss of nuclear rim staining. 1 Publication

    Organism-specific databases

    PharmGKBiPA31867.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 140139NTF2-related export protein 1PRO_0000194793Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9UKK6.
    PaxDbiQ9UKK6.
    PRIDEiQ9UKK6.

    PTM databases

    PhosphoSiteiQ9UKK6.

    Expressioni

    Gene expression databases

    BgeeiQ9UKK6.
    CleanExiHS_NXT1.
    GenevestigatoriQ9UKK6.

    Interactioni

    Subunit structurei

    Heterodimer with NXF1. Interacts (via NTF2 domain) with NXF1. Stablizes the NTF2 domain of NXF1 by heterodimerization. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Preferentially binds Ran-GTP. Associates with NXF2, NXF3 and NXF5. Does not bind nucleoporins (NPC) directly, its association to NPC is mediated by NXF1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NXF1Q9UBU94EBI-301889,EBI-398874
    NXF3Q9H4D53EBI-301889,EBI-750038
    Ranbp21Q9BP412EBI-301889,EBI-301896From a different organism.

    Protein-protein interaction databases

    BioGridi118875. 7 interactions.
    DIPiDIP-33075N.
    IntActiQ9UKK6. 8 interactions.
    MINTiMINT-216208.
    STRINGi9606.ENSP00000254998.

    Structurei

    Secondary structure

    1
    140
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 3025
    Helixi32 – 387
    Beta strandi39 – 479
    Beta strandi50 – 545
    Helixi55 – 6410
    Beta strandi68 – 7912
    Turni82 – 843
    Beta strandi90 – 10112
    Beta strandi107 – 11812
    Beta strandi121 – 1233
    Beta strandi125 – 13511
    Turni136 – 1394

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JKGX-ray1.90A1-140[»]
    1JN5X-ray2.80A1-140[»]
    ProteinModelPortaliQ9UKK6.
    SMRiQ9UKK6. Positions 2-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UKK6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 135120NTF2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 NTF2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG247320.
    HOGENOMiHOG000231890.
    HOVERGENiHBG018207.
    InParanoidiQ9UKK6.
    KOiK14285.
    OMAiYYSTMDK.
    OrthoDBiEOG7966J7.
    PhylomeDBiQ9UKK6.
    TreeFamiTF318944.

    Family and domain databases

    InterProiIPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    [Graphical view]
    PfamiPF02136. NTF2. 1 hit.
    [Graphical view]
    PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9UKK6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN    50
    AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK 100
    FEGNKQRDFN QNFILTAQAS PSNTVWKIAS DCFRFQDWAS 140
    Length:140
    Mass (Da):15,847
    Last modified:May 1, 2000 - v1
    Checksum:i358FA86AC3944594
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156957 mRNA. Translation: AAD54942.1.
    AL096677 Genomic DNA. Translation: CAC03437.2.
    BC000759 mRNA. Translation: AAH00759.1.
    BC002687 mRNA. Translation: AAH02687.1.
    BC003029 mRNA. Translation: AAH03029.1.
    BC003410 mRNA. Translation: AAH03410.1.
    CCDSiCCDS13150.1.
    RefSeqiNP_037380.1. NM_013248.2.
    UniGeneiHs.516933.

    Genome annotation databases

    EnsembliENST00000254998; ENSP00000254998; ENSG00000132661.
    GeneIDi29107.
    KEGGihsa:29107.
    UCSCiuc002wsx.1. human.

    Polymorphism databases

    DMDMi18203504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156957 mRNA. Translation: AAD54942.1 .
    AL096677 Genomic DNA. Translation: CAC03437.2 .
    BC000759 mRNA. Translation: AAH00759.1 .
    BC002687 mRNA. Translation: AAH02687.1 .
    BC003029 mRNA. Translation: AAH03029.1 .
    BC003410 mRNA. Translation: AAH03410.1 .
    CCDSi CCDS13150.1.
    RefSeqi NP_037380.1. NM_013248.2.
    UniGenei Hs.516933.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JKG X-ray 1.90 A 1-140 [» ]
    1JN5 X-ray 2.80 A 1-140 [» ]
    ProteinModelPortali Q9UKK6.
    SMRi Q9UKK6. Positions 2-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118875. 7 interactions.
    DIPi DIP-33075N.
    IntActi Q9UKK6. 8 interactions.
    MINTi MINT-216208.
    STRINGi 9606.ENSP00000254998.

    PTM databases

    PhosphoSitei Q9UKK6.

    Polymorphism databases

    DMDMi 18203504.

    Proteomic databases

    MaxQBi Q9UKK6.
    PaxDbi Q9UKK6.
    PRIDEi Q9UKK6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000254998 ; ENSP00000254998 ; ENSG00000132661 .
    GeneIDi 29107.
    KEGGi hsa:29107.
    UCSCi uc002wsx.1. human.

    Organism-specific databases

    CTDi 29107.
    GeneCardsi GC20P023331.
    HGNCi HGNC:15913. NXT1.
    MIMi 605811. gene.
    neXtProti NX_Q9UKK6.
    PharmGKBi PA31867.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG247320.
    HOGENOMi HOG000231890.
    HOVERGENi HBG018207.
    InParanoidi Q9UKK6.
    KOi K14285.
    OMAi YYSTMDK.
    OrthoDBi EOG7966J7.
    PhylomeDBi Q9UKK6.
    TreeFami TF318944.

    Miscellaneous databases

    ChiTaRSi NXT1. human.
    EvolutionaryTracei Q9UKK6.
    GeneWikii NXT1.
    GenomeRNAii 29107.
    NextBioi 52165.
    PROi Q9UKK6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9UKK6.
    CleanExi HS_NXT1.
    Genevestigatori Q9UKK6.

    Family and domain databases

    InterProi IPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    [Graphical view ]
    Pfami PF02136. NTF2. 1 hit.
    [Graphical view ]
    PROSITEi PS50177. NTF2_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export."
      Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M.
      Mol. Cell. Biol. 19:8616-8624(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung, Placenta and Uterus.
    4. "RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro."
      Ossareh-Nazari B., Maison C., Black B.E., Levesque L., Paschal B.M., Dargemont C.
      Mol. Cell. Biol. 20:4562-4571(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells."
      Guzik B.W., Levesque L., Prasad S., Bor Y.C., Black B.E., Paschal B.M., Rekosh D., Hammarskjold M.L.
      Mol. Cell. Biol. 21:2545-2554(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA."
      Katahira J., Inoue H., Hurt E., Yoneda Y.
      EMBO J. 28:556-567(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    8. "Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor."
      Fribourg S., Braun I.C., Izaurralde E., Conti E.
      Mol. Cell 8:645-656(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ILE-114 AND ARG-134.

    Entry informationi

    Entry nameiNXT1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UKK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3