Q9UKK6 (NXT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NTF2-related export protein 1

Alternative name(s):
Protein p15

Gene names

Name:

NXT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5. Ref.1 Ref.4 Ref.5 Ref.6
Subunit structure	Heterodimer with NXF1. Interacts (via NTF2 domain) with NXF1. Stablizes the NTF2 domain of NXF1 by heterodimerization. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Preferentially binds Ran-GTP. Associates with NXF2, NXF3 and NXF5. Does not bind nucleoporins (NPC) directly, its association to NPC is mediated by NXF1. Ref.7
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm.
Sequence similarities	Contains 1 NTF2 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Traceable author statement. Source: ProtInc nuclear pore Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NXF1	Q9UBU9	2	EBI-301889,EBI-398874
NXF3	Q9H4D5	3	EBI-301889,EBI-750038
Ranbp21	Q9BP41	2	EBI-301889,EBI-301896	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 140

140

NTF2-related export protein 1

PRO_0000194793

Regions

Domain

16 – 135

120

NTF2

Experimental info

Mutagenesis

114

1

I → R: 70% reduction in mRNA export activity. Ref.7

Mutagenesis

134

1

R → D: 57% reduction in mRNA export activity and loss of nuclear rim staining. Ref.7

Secondary structure

1

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140

Helix Strand Turn

Details...

Helix

6 – 30

25

Helix

32 – 38

7

Beta strand

39 – 47

9

Beta strand

50 – 54

5

Helix

55 – 64

10

Beta strand

68 – 79

12

Turn

82 – 84

3

Beta strand

90 – 101

12

Beta strand

107 – 118

12

Beta strand

121 – 123

3

Beta strand

125 – 135

11

Turn

136 – 139

4

Sequences

	Sequence		Length	Mass (Da)
	Q9UKK6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 358FA86AC3944594	FASTA	140	15,847
	10 20 30 40 50 60 MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE 70 80 90 100 110 120 FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK FEGNKQRDFN QNFILTAQAS 130 140 PSNTVWKIAS DCFRFQDWAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export." Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M. Mol. Cell. Biol. 19:8616-8624(1999) [PubMed: 10567585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Placenta and Uterus.
[4]	"RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro." Ossareh-Nazari B., Maison C., Black B.E., Levesque L., Paschal B.M., Dargemont C. Mol. Cell. Biol. 20:4562-4571(2000) [PubMed: 10848583] [Abstract] Cited for: FUNCTION.
[5]	"NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells." Guzik B.W., Levesque L., Prasad S., Bor Y.C., Black B.E., Paschal B.M., Rekosh D., Hammarskjold M.L. Mol. Cell. Biol. 21:2545-2554(2001) [PubMed: 11259602] [Abstract] Cited for: FUNCTION.
[6]	"Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA." Katahira J., Inoue H., Hurt E., Yoneda Y. EMBO J. 28:556-567(2009) [PubMed: 19165146] [Abstract] Cited for: FUNCTION.
[7]	"Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor." Fribourg S., Braun I.C., Izaurralde E., Conti E. Mol. Cell 8:645-656(2001) [PubMed: 11583626] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ILE-114 AND ARG-134.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF156957 mRNA. Translation: AAD54942.1.
AL096677 Genomic DNA. Translation: CAC03437.2.
BC000759 mRNA. Translation: AAH00759.1.
BC002687 mRNA. Translation: AAH02687.1.
BC003029 mRNA. Translation: AAH03029.1.
BC003410 mRNA. Translation: AAH03410.1.

IPI

IPI00007605.

RefSeq

NP_037380.1. NM_013248.2.

UniGene

Hs.516933.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JKG	X-ray	1.90	A	1-140	[»]
1JN5	X-ray	2.80	A	1-140	[»]

ProteinModelPortal

Q9UKK6.

SMR

Q9UKK6. Positions 2-140.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UKK6. 6 interactions.

MINT

MINT-216208.

STRING

Q9UKK6.

Polymorphism databases

DMDM

18203504.

Proteomic databases

PRIDE

Q9UKK6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000254998; ENSP00000254998; ENSG00000132661.

GeneID

29107.

KEGG

hsa:29107.

UCSC

uc002wsx.1. human.

Organism-specific databases

CTD

29107.

GeneCards

GC20P023279.

H-InvDB

HIX0015690.

HGNC

HGNC:15913. NXT1.

MIM

605811. gene.

neXtProt

NX_Q9UKK6.

PharmGKB

PA31867.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG11592.

GeneTree

ENSGT00510000046494.

HOGENOM

HBG315113.

HOVERGEN

HBG018207.

InParanoid

Q9UKK6.

OMA

STMDKRR.

OrthoDB

EOG4CG09P.

PhylomeDB

Q9UKK6.

Gene expression databases

ArrayExpress

Q9UKK6.

Bgee

Q9UKK6.

CleanEx

HS_NXT1.

Genevestigator

Q9UKK6.

GermOnline

ENSG00000132661. Homo sapiens.

Family and domain databases

InterPro

IPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]

KO

K14285.

Pfam

PF02136. NTF2. 1 hit.
[Graphical view]

PROSITE

PS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

52165.

SOURCE

Search...

Entry information

Entry name

NXT1_HUMAN

Accession

Primary (citable) accession number: Q9UKK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.