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Protein

NTF2-related export protein 1

Gene

NXT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.4 Publications

GO - Biological processi

  1. protein export from nucleus Source: Ensembl
  2. RNA export from nucleus Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
NTF2-related export protein 1
Alternative name(s):
Protein p15
Gene namesi
Name:NXT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15913. NXT1.

Subcellular locationi

  1. Nucleus
  2. Nucleus speckle Curated
  3. Cytoplasm

  4. Note: Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. nuclear pore Source: ProtInc
  3. nuclear speck Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi114 – 1141I → R: 70% reduction in mRNA export activity. 1 Publication
Mutagenesisi134 – 1341R → D: 57% reduction in mRNA export activity and loss of nuclear rim staining. 1 Publication

Organism-specific databases

PharmGKBiPA31867.

Polymorphism and mutation databases

BioMutaiNXT1.
DMDMi18203504.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 140139NTF2-related export protein 1PRO_0000194793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9UKK6.
PaxDbiQ9UKK6.
PRIDEiQ9UKK6.

PTM databases

PhosphoSiteiQ9UKK6.

Expressioni

Gene expression databases

BgeeiQ9UKK6.
CleanExiHS_NXT1.
GenevestigatoriQ9UKK6.

Interactioni

Subunit structurei

Heterodimer with NXF1. Interacts (via NTF2 domain) with NXF1. Stablizes the NTF2 domain of NXF1 by heterodimerization. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Preferentially binds Ran-GTP. Associates with NXF2, NXF3 and NXF5. Does not bind nucleoporins (NPC) directly, its association to NPC is mediated by NXF1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NXF1Q9UBU95EBI-301889,EBI-398874
NXF3Q9H4D56EBI-301889,EBI-750038
PAXIP1Q6ZW49-23EBI-301889,EBI-10236271
Ranbp21Q9BP412EBI-301889,EBI-301896From a different organism.
STAU1O957933EBI-301889,EBI-358174

Protein-protein interaction databases

BioGridi118875. 9 interactions.
DIPiDIP-33075N.
IntActiQ9UKK6. 10 interactions.
MINTiMINT-216208.
STRINGi9606.ENSP00000254998.

Structurei

Secondary structure

1
140
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 3025Combined sources
Helixi32 – 387Combined sources
Beta strandi39 – 479Combined sources
Beta strandi50 – 545Combined sources
Helixi55 – 6410Combined sources
Beta strandi68 – 7912Combined sources
Turni82 – 843Combined sources
Beta strandi90 – 10112Combined sources
Beta strandi107 – 11812Combined sources
Beta strandi121 – 1233Combined sources
Beta strandi125 – 13511Combined sources
Turni136 – 1394Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKGX-ray1.90A1-140[»]
1JN5X-ray2.80A1-140[»]
4WYKX-ray3.40B/D2-140[»]
ProteinModelPortaliQ9UKK6.
SMRiQ9UKK6. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKK6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 135120NTF2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG247320.
GeneTreeiENSGT00510000046494.
HOGENOMiHOG000231890.
HOVERGENiHBG018207.
InParanoidiQ9UKK6.
KOiK14285.
OMAiYYSTMDK.
OrthoDBiEOG7966J7.
PhylomeDBiQ9UKK6.
TreeFamiTF318944.

Family and domain databases

InterProiIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UKK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN
60 70 80 90 100
AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK
110 120 130 140
FEGNKQRDFN QNFILTAQAS PSNTVWKIAS DCFRFQDWAS
Length:140
Mass (Da):15,847
Last modified:May 1, 2000 - v1
Checksum:i358FA86AC3944594
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156957 mRNA. Translation: AAD54942.1.
AL096677 Genomic DNA. Translation: CAC03437.2.
BC000759 mRNA. Translation: AAH00759.1.
BC002687 mRNA. Translation: AAH02687.1.
BC003029 mRNA. Translation: AAH03029.1.
BC003410 mRNA. Translation: AAH03410.1.
CCDSiCCDS13150.1.
RefSeqiNP_037380.1. NM_013248.2.
UniGeneiHs.516933.

Genome annotation databases

EnsembliENST00000254998; ENSP00000254998; ENSG00000132661.
GeneIDi29107.
KEGGihsa:29107.
UCSCiuc002wsx.1. human.

Polymorphism and mutation databases

BioMutaiNXT1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156957 mRNA. Translation: AAD54942.1.
AL096677 Genomic DNA. Translation: CAC03437.2.
BC000759 mRNA. Translation: AAH00759.1.
BC002687 mRNA. Translation: AAH02687.1.
BC003029 mRNA. Translation: AAH03029.1.
BC003410 mRNA. Translation: AAH03410.1.
CCDSiCCDS13150.1.
RefSeqiNP_037380.1. NM_013248.2.
UniGeneiHs.516933.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKGX-ray1.90A1-140[»]
1JN5X-ray2.80A1-140[»]
4WYKX-ray3.40B/D2-140[»]
ProteinModelPortaliQ9UKK6.
SMRiQ9UKK6. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118875. 9 interactions.
DIPiDIP-33075N.
IntActiQ9UKK6. 10 interactions.
MINTiMINT-216208.
STRINGi9606.ENSP00000254998.

PTM databases

PhosphoSiteiQ9UKK6.

Polymorphism and mutation databases

BioMutaiNXT1.
DMDMi18203504.

Proteomic databases

MaxQBiQ9UKK6.
PaxDbiQ9UKK6.
PRIDEiQ9UKK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254998; ENSP00000254998; ENSG00000132661.
GeneIDi29107.
KEGGihsa:29107.
UCSCiuc002wsx.1. human.

Organism-specific databases

CTDi29107.
GeneCardsiGC20P023331.
HGNCiHGNC:15913. NXT1.
MIMi605811. gene.
neXtProtiNX_Q9UKK6.
PharmGKBiPA31867.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG247320.
GeneTreeiENSGT00510000046494.
HOGENOMiHOG000231890.
HOVERGENiHBG018207.
InParanoidiQ9UKK6.
KOiK14285.
OMAiYYSTMDK.
OrthoDBiEOG7966J7.
PhylomeDBiQ9UKK6.
TreeFamiTF318944.

Miscellaneous databases

ChiTaRSiNXT1. human.
EvolutionaryTraceiQ9UKK6.
GeneWikiiNXT1.
GenomeRNAii29107.
NextBioi52165.
PROiQ9UKK6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UKK6.
CleanExiHS_NXT1.
GenevestigatoriQ9UKK6.

Family and domain databases

InterProiIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export."
    Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M.
    Mol. Cell. Biol. 19:8616-8624(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung, Placenta and Uterus.
  4. "RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro."
    Ossareh-Nazari B., Maison C., Black B.E., Levesque L., Paschal B.M., Dargemont C.
    Mol. Cell. Biol. 20:4562-4571(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells."
    Guzik B.W., Levesque L., Prasad S., Bor Y.C., Black B.E., Paschal B.M., Rekosh D., Hammarskjold M.L.
    Mol. Cell. Biol. 21:2545-2554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA."
    Katahira J., Inoue H., Hurt E., Yoneda Y.
    EMBO J. 28:556-567(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  8. "Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor."
    Fribourg S., Braun I.C., Izaurralde E., Conti E.
    Mol. Cell 8:645-656(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ILE-114 AND ARG-134.

Entry informationi

Entry nameiNXT1_HUMAN
AccessioniPrimary (citable) accession number: Q9UKK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.