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Q9UKK6 (NXT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NTF2-related export protein 1
Alternative name(s):
Protein p15
Gene names
Name:NXT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length140 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. Ref.1 Ref.4 Ref.5 Ref.6

Subunit structure

Heterodimer with NXF1. Interacts (via NTF2 domain) with NXF1. Stablizes the NTF2 domain of NXF1 by heterodimerization. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Preferentially binds Ran-GTP. Associates with NXF2, NXF3 and NXF5. Does not bind nucleoporins (NPC) directly, its association to NPC is mediated by NXF1. Ref.8

Subcellular location

Nucleus. Nucleus speckle Probable. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm.

Sequence similarities

Contains 1 NTF2 domain.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NXF1Q9UBU92EBI-301889,EBI-398874
NXF3Q9H4D53EBI-301889,EBI-750038
Ranbp21Q9BP412EBI-301889,EBI-301896From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 140139NTF2-related export protein 1
PRO_0000194793

Regions

Domain16 – 135120NTF2

Amino acid modifications

Modified residue21N-acetylalanine Ref.7

Experimental info

Mutagenesis1141I → R: 70% reduction in mRNA export activity. Ref.8
Mutagenesis1341R → D: 57% reduction in mRNA export activity and loss of nuclear rim staining. Ref.8

Secondary structure

...................... 140
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9UKK6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 358FA86AC3944594

FASTA14015,847
        10         20         30         40         50         60 
MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE 

        70         80         90        100        110        120 
FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK FEGNKQRDFN QNFILTAQAS 

       130        140 
PSNTVWKIAS DCFRFQDWAS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export."
Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M.
Mol. Cell. Biol. 19:8616-8624(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung, Placenta and Uterus.
[4]"RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro."
Ossareh-Nazari B., Maison C., Black B.E., Levesque L., Paschal B.M., Dargemont C.
Mol. Cell. Biol. 20:4562-4571(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells."
Guzik B.W., Levesque L., Prasad S., Bor Y.C., Black B.E., Paschal B.M., Rekosh D., Hammarskjold M.L.
Mol. Cell. Biol. 21:2545-2554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA."
Katahira J., Inoue H., Hurt E., Yoneda Y.
EMBO J. 28:556-567(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[8]"Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor."
Fribourg S., Braun I.C., Izaurralde E., Conti E.
Mol. Cell 8:645-656(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, MUTAGENESIS OF ILE-114 AND ARG-134.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF156957 mRNA. Translation: AAD54942.1.
AL096677 Genomic DNA. Translation: CAC03437.2.
BC000759 mRNA. Translation: AAH00759.1.
BC002687 mRNA. Translation: AAH02687.1.
BC003029 mRNA. Translation: AAH03029.1.
BC003410 mRNA. Translation: AAH03410.1.
RefSeqNP_037380.1. NM_013248.2.
UniGeneHs.516933.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKGX-ray1.90A1-140[»]
1JN5X-ray2.80A1-140[»]
ProteinModelPortalQ9UKK6.
SMRQ9UKK6. Positions 2-140.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118875. 7 interactions.
IntActQ9UKK6. 7 interactions.
MINTMINT-216208.
STRING9606.ENSP00000254998.

PTM databases

PhosphoSiteQ9UKK6.

Polymorphism databases

DMDM18203504.

Proteomic databases

PaxDbQ9UKK6.
PRIDEQ9UKK6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254998; ENSP00000254998; ENSG00000132661.
GeneID29107.
KEGGhsa:29107.
UCSCuc002wsx.1. human.

Organism-specific databases

CTD29107.
GeneCardsGC20P023331.
HGNCHGNC:15913. NXT1.
MIM605811. gene.
neXtProtNX_Q9UKK6.
PharmGKBPA31867.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG247320.
HOGENOMHOG000231890.
HOVERGENHBG018207.
InParanoidQ9UKK6.
KOK14285.
OMAYYSTMDK.
OrthoDBEOG7966J7.
PhylomeDBQ9UKK6.
TreeFamTF318944.

Gene expression databases

BgeeQ9UKK6.
CleanExHS_NXT1.
GenevestigatorQ9UKK6.

Family and domain databases

InterProIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNXT1. human.
EvolutionaryTraceQ9UKK6.
GeneWikiNXT1.
GenomeRNAi29107.
NextBio52165.
PROQ9UKK6.
SOURCESearch...

Entry information

Entry nameNXT1_HUMAN
AccessionPrimary (citable) accession number: Q9UKK6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 2000
Last modified: March 19, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM