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Reviewed, UniProtKB/Swiss-Prot Q9UKJ8 (ADA21_HUMAN)

Last modified October 13, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Disintegrin and metalloproteinase domain-containing protein 21
      Short name=ADAM 21
    EC=3.4.24.-
Gene names
Name: ADAM21
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients.

Cofactor

Binds 1 zinc ion per subunit Potential.

Subcellular location

Membrane; Single-pass type I membrane protein.

Domain

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.

Miscellaneous

May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

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Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

single fertilization Ref.3

Traceable author statement. Source: ProtInc

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Propeptide32 – 196165 Potential
PRO_0000029108
Chain197 – 722526Disintegrin and metalloproteinase domain-containing protein 21
PRO_0000029109

Regions

Topological domain197 – 681485Extracellular Potential
Transmembrane682 – 70221 Potential
Topological domain703 – 72220Cytoplasmic Potential
Domain208 – 398191Peptidase M12B
Domain406 – 49287Disintegrin
Domain634 – 66330EGF-like
Motif171 – 1788Cysteine switch By similarity
Compositional bias213 – 2164Poly-Val
Compositional bias493 – 633141Cys-rich

Sites

Active site3421 Potential
Metal binding1731Zinc; in inhibited form By similarity
Metal binding3411Zinc; catalytic Potential
Metal binding3451Zinc; catalytic Potential
Metal binding3511Zinc; catalytic Potential

Amino acid modifications

Glycosylation1641N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation3771N-linked (GlcNAc...) Potential
Glycosylation4371N-linked (GlcNAc...) Potential
Glycosylation4781N-linked (GlcNAc...) Potential
Glycosylation5461N-linked (GlcNAc...) Potential
Glycosylation6001N-linked (GlcNAc...) Potential
Disulfide bond316 ↔ 393 By similarity
Disulfide bond356 ↔ 378 By similarity
Disulfide bond358 ↔ 363 By similarity
Disulfide bond464 ↔ 484 By similarity
Disulfide bond634 ↔ 645 By similarity
Disulfide bond639 ↔ 651 By similarity
Disulfide bond653 ↔ 662 By similarity

Experimental info

Sequence conflict1811A → G in AAC52042. Ref.3
Sequence conflict2471Q → K in AAC52042. Ref.3
Sequence conflict3451H → Y in AAC52042. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Q9UKJ8-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 25DFD896F8A1876A

FASTA72280,820
        10         20         30         40         50         60 
MAVDGTLVYI RVTLLLLWLG VFLSISGYCQ AGPSQHFTSP EVVIPLKVIS RGRSAKAPGW 

        70         80         90        100        110        120 
LSYSLRFGGQ KHVVHMRVKK LLVSRHLPVF TYTDERALLE DQLFIPDDCY YHGYVEGAPE 

       130        140        150        160        170        180 
SLVVFSACFG GFRGVLKISG LTYEIEPIRH SATFEHLVYK VNSNETQFPA MRCGLTEKEV 

       190        200        210        220        230        240 
ARQQLEFEEA ENSALEPKSA GDWWTHAWFL ELVVVVNHDF FIYSQSNISK VQEDVFLVVN 

       250        260        270        280        290        300 
IVDSMYQQLG TYIILIGIEI WNQGNVFPMT SIEQVLNDFS QWKQISLSQL QHDAAHMFIK 

       310        320        330        340        350        360 
NSLISILGLA YVAGICRPPI DCGVDNFQGD TWSLFANTVA HELGHTLGMQ HDEEFCFCGE 

       370        380        390        400        410        420 
RGCIMNTFRV PAEKFTNCSY ADFMKTTLNQ GSCLHNPPRL GEIFMLKRCG NGVVEREEQC 

       430        440        450        460        470        480 
DCGSVQQCEQ DACCLLNCTL RPGAACAFGL CCKDCKFMPS GELCRQEVNE CDLPEWCNGT 

       490        500        510        520        530        540 
SHQCPEDRYV QDGIPCSDSA YCYQKRCNNH DQHCREIFGK DAKSASQNCY KEINSQGNRF 

       550        560        570        580        590        600 
GHCGINGTTY LKCHISDVFC GRVQCENVRD IPLLQDHFTL QHTHINGVTC WGIDYHLRMN 

       610        620        630        640        650        660 
ISDIGEVKDG TVCGPGKICI HKKCVSLSVL SHVCLPETCN MKGICNNKHH CHCGYGWSPP 

       670        680        690        700        710        720 
YCQHRGYGGS IDSGPASAKR GVFLPLIVIP SLSVLTFLFT VGLLMYLRQC SGPKETKAHS 


SG

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References

« Hide 'large scale' references
[1]"The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries."
Poindexter K., Nelson N., DuBose R.F., Black R.A., Cerretti D.P.
Gene 237:61-70(1999) [PubMed: 10524237] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha."
Hooft van Huijsduijnen R.
Gene 206:273-282(1998) [PubMed: 9469942] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-722.
Tissue: Testis.

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Cross-references

Sequence databases

AF158644 Genomic DNA. Translation: AAD55255.1.
BC109024 mRNA. Translation: AAI09025.1.
BC109025 mRNA. Translation: AAI09026.1.
AF029900 mRNA. Translation: AAC52042.1.
IPIIPI00007596.
RefSeqNP_003804.1.
UniGeneHs.178748

3D structure databases

HSSPHSSP built from PDB template 1FVL based on UniProtKB P18619.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9UKJ8.

Protein family/group databases

MEROPSM12.234.

Proteomic databases

PRIDEQ9UKJ8.

Genome annotation databases

EnsemblENST00000267499; ENSP00000267499; ENSG00000139985; Homo sapiens. [Genome view]
GeneID8747.
KEGGhsa:8747.
UCSCuc001xmd.1. human.

Organism-specific databases

CTD8747.
GeneCardsGC14P069993.
HGNCHGNC:200. ADAM21.
MIM603713. gene.
PharmGKBPA24517.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9UKJ8.
HOVERGENQ9UKJ8.

Gene expression databases

ArrayExpressQ9UKJ8.
BgeeQ9UKJ8.
CleanExHS_ADAM21.
GenevestigatorQ9UKJ8.
GermOnlineENSG00000139985. Homo sapiens.

Family and domain databases

InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR013032. EGF-like_reg_CS.
IPR001818. Pept_M10A_M12B.
IPR006025. Pept_M_Zn_BS.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
Gene3DG3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
ProDomPD000664. Disintegrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00546. CYSTEINE_SWITCH. False negative.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. False negative.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio32817.
SOURCESearch...

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Entry information

Entry nameADA21_HUMAN
AccessionPrimary (citable) accession number: Q9UKJ8
Secondary accession number(s): O43507, Q2VPC6, Q32MR0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: October 13, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents