Q9UKI2 (BORG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cdc42 effector protein 3

Alternative name(s):
Binder of Rho GTPases 2
MSE55-related Cdc42-binding protein

Gene names

Name:	CDC42EP3
Synonyms:	BORG2, CEP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Ref.1 Ref.2
Subunit structure	Interacts with RHOQ and CDC42, in a GTP-dependent manner, and with SEPT7. Ref.1 Ref.10
Subcellular location	Endomembrane system; Peripheral membrane protein. Cytoplasm › cytoskeleton Ref.2.
Tissue specificity	Highly expressed in the heart and weakly in the brain. Ref.1
Sequence similarities	Belongs to the BORG/CEP family. Contains 1 CRIB domain.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cytoplasm Cytoskeleton Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Traceable author statement Ref.2. Source: ProtInc
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: LIFEdb cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cytoskeletal regulatory protein binding Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Cdc42 effector protein 3		PRO_0000212651
Regions
	Domain	31 – 45	15	CRIB
Amino acid modifications
	Modified residue	63	1	Phosphotyrosine By similarity
	Modified residue	91	1	Phosphoserine By similarity
	Modified residue	108	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	56	1	I → N in AAD16888. Ref.2
	Sequence conflict	233	1	S → F in AAC71773. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9UKI2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C31CDBE151C161ED	FASTA	254	27,678
	10 20 30 40 50 60 MPAKTPIYLK AANNKKGKKF KLRDILSPDM ISPPLGDFRH TIHIGKEGQH DVFGDISFLQ 70 80 90 100 110 120 GNYELLPGNQ EKAHLGQFPG HNEFFRANST SDSVFTETPS PVLKNAISLP TIGGSQALML 130 140 150 160 170 180 PLLSPVTFNS KQESFGPAKL PRLSCEPVME EKAQEKSSLL ENGTVHQGDT SWGSSGSASQ 190 200 210 220 230 240 SSQGRDSHSS SLSEQYPDWP AEDMFDHPTP CELIKGKTKS EESLSDLTGS LLSLQLDLGP 250 SLLDEVLNVM DKNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins." Joberty G., Perlungher R.R., Macara I.G. Mol. Cell. Biol. 19:6585-6597(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH RHOQ AND CDC42. Tissue: Heart.
[2]	"A new family of Cdc42 effector proteins, CEPs, function in fibroblast and epithelial cell shape changes." Hirsch D.S., Pirone D.M., Burbelo P.D. J. Biol. Chem. 276:875-883(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Tissue: Uterus.
[3]	"MSE55-related Cdc42-binding protein." Alberts A.S. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[7]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[10]	"Borg proteins control septin organization and are negatively regulated by Cdc42." Joberty G., Perlungher R.R., Sheffield P.J., Kinoshita M., Noda M., Haystead T., Macara I.G. Nat. Cell Biol. 3:861-866(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SEPT7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF164118 mRNA. Translation: AAD48815.1. AF104857 mRNA. Translation: AAD16888.1. AF094521 mRNA. Translation: AAC71773.1. AL136842 mRNA. Translation: CAB66776.1. BT007190 mRNA. Translation: AAP35854.1. AK313483 mRNA. Translation: BAG36267.1. AC006369 Genomic DNA. Translation: AAX93072.1. CH471053 Genomic DNA. Translation: EAX00390.1. BC019270 mRNA. Translation: AAH19270.1.
IPI	IPI00294391.
PIR	T46465.
RefSeq	NP_001257365.1. NM_001270436.1. NP_001257366.1. NM_001270437.1. NP_001257367.1. NM_001270438.1. NP_006440.2. NM_006449.4.
UniGene	Hs.369574.
3D structure databases
ProteinModelPortal	Q9UKI2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UKI2. 3 interactions.
MINT	MINT-1397043.
STRING	9606.ENSP00000295324.
PTM databases
PhosphoSite	Q9UKI2.
Polymorphism databases
DMDM	71152340.
Proteomic databases
PaxDb	Q9UKI2.
PRIDE	Q9UKI2.
Protocols and materials databases
DNASU	10602.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295324; ENSP00000295324; ENSG00000163171.
GeneID	10602.
KEGG	hsa:10602.
UCSC	uc002rqi.1. human.
Organism-specific databases
CTD	10602.
GeneCards	GC02M037782.
HGNC	HGNC:16943. CDC42EP3.
HPA	HPA034986.
MIM	606133. gene.
neXtProt	NX_Q9UKI2.
PharmGKB	PA38429.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46217.
HOGENOM	HOG000233908.
HOVERGEN	HBG052803.
InParanoid	Q9UKI2.
OMA	NSTSDSM.
OrthoDB	EOG45DWQF.
Gene expression databases
ArrayExpress	Q9UKI2.
Bgee	Q9UKI2.
CleanEx	HS_CDC42EP3.
Genevestigator	Q9UKI2.
GermOnline	ENSG00000163171. Homo sapiens.
Family and domain databases
InterPro	IPR000095. PAK_box_Rho-bd. [Graphical view]
Pfam	PF00786. PBD. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDC42EP3. human.
GenomeRNAi	10602.
NextBio	40262.
SOURCE	Search...

Entry information

Entry name

BORG2_HUMAN

Accession

Primary (citable) accession number: Q9UKI2
Secondary accession number(s): B2R8S0, O95353, Q9UQJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.