Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9UKF5 (ADA29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 29

Short name=ADAM 29
Alternative name(s):
Cancer/testis antigen 73
Short name=CT73
Gene names
Name:ADAM29
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in spermatogenesis and fertilization. Seems to be a non catalytic metalloprotease-like protein.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed specifically in testes.

Involvement in disease

Has been found to be frequently mutated in melanoma. ADAM7 mutations may play a role in melanoma progression and metastasis.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q9UKF5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q9UKF5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     750-803: Missing.
Isoform Gamma (identifier: Q9UKF5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     763-787: VTPSQSQPRVMPSQSQPPVMPSQSH → QNLFLFSFSISDCVLNFRLLYLQAT
     788-820: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 193175 By similarity
PRO_0000029134
Chain194 – 820627Disintegrin and metalloproteinase domain-containing protein 29
PRO_0000029135

Regions

Topological domain194 – 674481Extracellular Potential
Transmembrane675 – 69521Helical; Potential
Topological domain696 – 820125Cytoplasmic Potential
Domain198 – 390193Peptidase M12B
Domain397 – 48387Disintegrin
Domain625 – 65430EGF-like
Repeat739 – 74791
Repeat748 – 75692
Repeat757 – 76593
Repeat766 – 77494
Repeat775 – 78395
Repeat784 – 79296
Repeat793 – 80197
Repeat802 – 81098
Repeat811 – 81999
Region739 – 819819 X 9 AA approximate repeats
Compositional bias484 – 624141Cys-rich
Compositional bias669 – 6757Poly-Lys

Amino acid modifications

Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Glycosylation3681N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Glycosylation4691N-linked (GlcNAc...) Potential
Glycosylation5381N-linked (GlcNAc...) Potential
Glycosylation5451N-linked (GlcNAc...) Potential
Glycosylation5581N-linked (GlcNAc...) Potential
Glycosylation5641N-linked (GlcNAc...) Potential
Disulfide bond307 ↔ 384 By similarity
Disulfide bond347 ↔ 369 By similarity
Disulfide bond349 ↔ 354 By similarity
Disulfide bond455 ↔ 475 By similarity
Disulfide bond625 ↔ 636 By similarity
Disulfide bond630 ↔ 642 By similarity
Disulfide bond644 ↔ 653 By similarity

Natural variations

Alternative sequence750 – 80354Missing in isoform Beta.
VSP_005491
Alternative sequence763 – 78725VTPSQ…PSQSH → QNLFLFSFSISDCVLNFRLL YLQAT in isoform Gamma.
VSP_005492
Alternative sequence788 – 82033Missing in isoform Gamma.
VSP_005493
Natural variant311P → L in a colorectal cancer sample; somatic mutation. Ref.7
VAR_036148
Natural variant721L → F in a melanoma cell line. Ref.6
VAR_066322
Natural variant891I → M in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066323
Natural variant1111E → K in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066324
Natural variant1121S → F in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066325
Natural variant1151S → F in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066326
Natural variant1311D → N in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066327
Natural variant1761E → K in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066328
Natural variant2051V → I in a colorectal cancer sample; somatic mutation. Ref.7
VAR_036149
Natural variant2341S → F in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066329
Natural variant2571I → F in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066330
Natural variant3051G → E in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066331
Natural variant3451D → N in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066332
Natural variant4031G → D in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066333
Natural variant4341G → D in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066334
Natural variant5031E → K in a cutaneous metastatic melanoma sample; somatic mutation; increases the adhesion of melanoma cells to collagens I and IV. Ref.6
VAR_066335
Natural variant5331H → Y in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066336

Experimental info

Sequence conflict1961H → Y in AAF22163. Ref.2
Sequence conflict7441P → H in AAF22163. Ref.2
Sequence conflict7481S → Y in AAF22163. Ref.2
Sequence conflict7531Q → R in AAF03777. Ref.1
Sequence conflict7641T → M in AAF03777. Ref.1
Sequence conflict769 – 7735QPRVM → HPQLT in AAF03777. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified April 17, 2007. Version 3.
Checksum: AA2FB11342A884C9

FASTA82092,759
        10         20         30         40         50         60 
MKMLLLLHCL GVFLSCSGHI QDEHPQYHSP PDVVIPVRIT GTTRGMTPPG WLSYILPFGG 

        70         80         90        100        110        120 
QKHIIHIKVK KLLFSKHLPV FTYTDQGAIL EDQPFVQNNC YYHGYVEGDP ESLVSLSTCF 

       130        140        150        160        170        180 
GGFQGILQIN DFAYEIKPLA FSTTFEHLVY KMDSEEKQFS TMRSGFMQNE ITCRMEFEEI 

       190        200        210        220        230        240 
DNSTQKQSSY VGWWIHFRIV EIVVVIDNYL YIRYERNDSK LLEDLYVIVN IVDSILDVIG 

       250        260        270        280        290        300 
VKVLLFGLEI WTNKNLIVVD DVRKSVHLYC KWKSENITPR MQHDTSHLFT TLGLRGLSGI 

       310        320        330        340        350        360 
GAFRGMCTPH RSCAIVTFMN KTLGTFSIAV AHHLGHNLGM NHDEDTCRCS QPRCIMHEGN 

       370        380        390        400        410        420 
PPITKFSNCS YGDFWEYTVE RTKCLLETVH TKDIFNVKRC GNGVVEEGEE CDCGPLKHCA 

       430        440        450        460        470        480 
KDPCCLSNCT LTDGSTCAFG LCCKDCKFLP SGKVCRKEVN ECDLPEWCNG TSHKCPDDFY 

       490        500        510        520        530        540 
VEDGIPCKER GYCYEKSCHD RNEQCRRIFG AGANTASETC YKELNTLGDR VGHCGIKNAT 

       550        560        570        580        590        600 
YIKCNISDVQ CGRIQCENVT EIPNMSDHTT VHWARFNDIM CWSTDYHLGM KGPDIGEVKD 

       610        620        630        640        650        660 
GTECGIDHIC IHRHCVHITI LNSNCSPAFC NKRGICNNKH HCHCNYLWDP PNCLIKGYGG 

       670        680        690        700        710        720 
SVDSGPPPKR KKKKKFCYLC ILLLIVLFIL LCCLYRLCKK SKPIKKQQDV QTPSAKEEEK 

       730        740        750        760        770        780 
IQRRPHELPP QSQPWVMPSQ SQPPVTPSQS HPQVMPSQSQ PPVTPSQSQP RVMPSQSQPP 

       790        800        810        820 
VMPSQSHPQL TPSQSQPPVT PSQRQPQLMP SQSQPPVTPS 

« Hide

Isoform Beta [UniParc].

Checksum: ACA4BEDFA700ABA5
Show »

FASTA76686,983
Isoform Gamma [UniParc].

Checksum: 2B0E5E7C496B8C9D
Show »

FASTA78789,460

References

« Hide 'large scale' references
[1]"Isolation of two novel metalloproteinase-disintegrin (ADAM) cDNAs that show testis-specific gene expression."
Cerretti D.P., DuBose R.F., Black R.A., Nelson N.
Biochem. Biophys. Res. Commun. 263:810-815(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA).
Tissue: Testis.
[2]"Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4."
Xu R., Cai J., Xu T., Zhou W., Ying B., Deng K., Zhao S., Li C.
Genomics 62:537-539(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
Tissue: Testis.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA).
Tissue: Testis.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Analysis of the disintegrin-metalloproteinases family reveals ADAM29 and ADAM7 are often mutated in melanoma."
Wei X., Moncada-Pazos A., Cal S., Soria-Valles C., Gartner J., Rudloff U., Lin J.C., Rosenberg S.A., Lopez-Otin C., Samuels Y.
Hum. Mutat. 32:E2148-E2175(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN CUTANEOUS MELANOMA, VARIANTS PHE-72; MET-89; LYS-111; PHE-112; PHE-115; ASN-131; LYS-176; PHE-234; PHE-257; GLU-305; ASN-345; ASP-403; ASP-434; LYS-503 AND TYR-533, CHARACTERIZATION OF VARIANTS LYS-111; PHE-112; PHE-115; PHE-257; ASP-434 AND LYS-503.
[7]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-31 AND ILE-205.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF171929 mRNA. Translation: AAF03777.1.
AF171930 mRNA. Translation: AAF03778.1.
AF171931 mRNA. Translation: AAF03779.1.
AF134708 mRNA. Translation: AAF22163.1.
AK292410 mRNA. Translation: BAF85099.1.
AC105914 Genomic DNA. Translation: AAY41055.1.
CH471056 Genomic DNA. Translation: EAX04727.1.
CH471056 Genomic DNA. Translation: EAX04728.1.
RefSeqNP_001124175.1. NM_001130703.1.
NP_001124176.1. NM_001130704.1.
NP_001124177.1. NM_001130705.1.
NP_001265054.1. NM_001278125.1.
NP_001265055.1. NM_001278126.1.
NP_001265056.1. NM_001278127.1.
NP_055084.3. NM_014269.4.
XP_005262761.1. XM_005262704.1.
XP_005262762.1. XM_005262705.2.
UniGeneHs.126838.

3D structure databases

ProteinModelPortalQ9UKF5.
SMRQ9UKF5. Positions 193-659.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-252736.
STRING9606.ENSP00000352177.

Protein family/group databases

MEROPSM12.981.

PTM databases

PhosphoSiteQ9UKF5.

Polymorphism databases

DMDM145559438.

Proteomic databases

PaxDbQ9UKF5.
PRIDEQ9UKF5.

Protocols and materials databases

DNASU11086.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359240; ENSP00000352177; ENSG00000168594. [Q9UKF5-1]
ENST00000404450; ENSP00000384229; ENSG00000168594. [Q9UKF5-1]
ENST00000445694; ENSP00000414544; ENSG00000168594. [Q9UKF5-1]
ENST00000514159; ENSP00000423517; ENSG00000168594. [Q9UKF5-1]
GeneID11086.
KEGGhsa:11086.
UCSCuc003iuc.3. human. [Q9UKF5-1]

Organism-specific databases

CTD11086.
GeneCardsGC04P175751.
HGNCHGNC:207. ADAM29.
HPAHPA014159.
MIM604778. gene.
neXtProtNX_Q9UKF5.
PharmGKBPA24524.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG297875.
HOGENOMHOG000230883.
HOVERGENHBG006978.
InParanoidQ9UKF5.
KOK16069.
OMASCAIVTF.
OrthoDBEOG7KWSGW.
PhylomeDBQ9UKF5.
TreeFamTF314733.

Gene expression databases

ArrayExpressQ9UKF5.
BgeeQ9UKF5.
CleanExHS_ADAM29.
GenevestigatorQ9UKF5.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSADAM29. human.
GenomeRNAi11086.
NextBio42152.
PROQ9UKF5.
SOURCESearch...

Entry information

Entry nameADA29_HUMAN
AccessionPrimary (citable) accession number: Q9UKF5
Secondary accession number(s): Q4W5F3 expand/collapse secondary AC list , Q9UHP1, Q9UKF3, Q9UKF4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM