Q9UKB5 (AJAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adherens junction-associated protein 1

Alternative name(s):
Membrane protein shrew-1

Gene names

Name:	AJAP1
Synonyms:	MOT8, SHREW1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in cell adhesion and cell migration. Ref.5 Ref.8
Subunit structure	Forms a complex with CDH1 and CTNNB1; interacts directly with CTNNB1. Interacts with AP1M2 and BSG/CD147. Ref.1 Ref.7 Ref.8
Subcellular location	Basolateral cell membrane; Single-pass type III membrane protein. Apical cell membrane; Single-pass type III membrane protein. Cell junction › adherens junction. Note: Mainly basolateral. Localization is mediated by AP1M2. Ref.1 Ref.7
Tissue specificity	Expressed in uterus and pancreas (at protein level). Ref.1
Post-translational modification	Thr-237 and Ser-239 may be phosphorylated; however as this position is probably extracellular, the in vivo relevance is not proven. Ref.6
Sequence caution	The sequence CAB41245.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB41246.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	adherens junction Inferred from electronic annotation. Source: UniProtKB-SubCell apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Adherens junction-associated protein 1		PRO_0000284801
Regions
	Topological domain	1 – 282	282	Extracellular Potential
	Transmembrane	283 – 303	21	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	304 – 411	108	Cytoplasmic Potential
	Region	303 – 411	109	Targeting signals
	Compositional bias	175 – 237	63	Thr-rich
Amino acid modifications
	Modified residue	237	1	Phosphothreonine Ref.6
	Modified residue	239	1	Phosphoserine Ref.6
Natural variations
	Natural variant	263	1	G → R. Ref.5 Corresponds to variant rs242056 [ dbSNP | Ensembl ].		VAR_031821
Experimental info
	Mutagenesis	303	1	L → A: Mistargeting to the apical membrane. Ref.7
	Mutagenesis	350	1	Y → A: Mistargeting to the apical membrane. Ref.7
	Mutagenesis	368	1	Y → A: Mistargeting to the apical membrane. Ref.7
	Mutagenesis	380	1	Y → A: Mistargeting to the apical membrane. Ref.7
	Mutagenesis	396 – 397	2	LI → HV: Mistargeting to the apical membrane. Ref.7
	Mutagenesis	396	1	L → A: Mistargeting to the apical membrane. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9UKB5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 42C1C5F56B9FC975	FASTA	411	44,536
	10 20 30 40 50 60 MWIQQLLGLS SMSIRWPGRP LGSHAWILIA MFQLAVDLPA CEALGPGPEF WLLPRSPPRP 70 80 90 100 110 120 PRLWSFRSGQ PARVPAPVWS PRPPRVERIH GQMQMPRARR AHRPRDQAAA LVPKAGLAKP 130 140 150 160 170 180 PAAAKSSPSL ASSSSSSSSA VAGGAPEQQA LLRRGKRHLQ GDGLSSFDSR GSRPTTETEF 190 200 210 220 230 240 IAWGPTGDEE ALESNTFPGV YGPTTVSILQ TRKTTVAATT TTTTTATPMT LQTKGFTESL 250 260 270 280 290 300 DPRRRIPGGV STTEPSTSPS NNGEVTQPPR ILGEASGLAV HQIITITVSL IMVIAALITT 310 320 330 340 350 360 LVLKNCCAQS GNTRRNSHQR KTNQQEESCQ NLTDFPSARV PSSLDIFTAY NETLQCSHEC 370 380 390 400 410 VRASVPVYTD ETLHSTTGEY KSTFNGNRPS SSDRHLIPVA FVSEKWFEIS C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel membrane protein shrew-1 targets to cadherin-mediated junctions in polarized epithelial cells." Bharti S., Handrow-Metzmacher H., Zickenheiner S., Zeitvogel A., Baumann R., Starzinski-Powitz A. Mol. Biol. Cell 15:397-406(2004) [PubMed: 14595118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH CDH1 AND CTNNB1. Tissue: Brain.
[2]	"Identification of genes differentially expressed in HT1080 human fibrosarcoma cells selected for invasive capacity in vitro." Kudoh S., Chung I.M., Tsuiji M., Tsuji T., Irimura T. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibrosarcoma.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Rhodes S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 179-411.
[5]	"The SHREW1 gene, frequently deleted in oligodendrogliomas, functions to inhibit cell adhesion and migration." McDonald J.M., Dunlap S., Cogdell D., Dunmire V., Wei Q., Starzinski-Powitz A., Sawaya R., Bruner J., Fuller G.N., Aldape K., Zhang W. Cancer Biol. Ther. 5:300-304(2006) [PubMed: 16410724] [Abstract] Cited for: FUNCTION, VARIANT ARG-263.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-239, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs." Jakob V., Schreiner A., Tikkanen R., Starzinski-Powitz A. Mol. Biol. Cell 17:3397-3408(2006) [PubMed: 16707570] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-303; TYR-350; TYR-368; TYR-380; LEU-396 AND 396-LEU--ILE-397, INTERACTION WITH AP1M2.
[8]	"junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147." Schreiner A., Ruonala M., Jakob V., Suthaus J., Boles E., Wouters F., Starzinski-Powitz A. Mol. Biol. Cell 18:1272-1281(2007) [PubMed: 17267690] [Abstract] Cited for: FUNCTION, INTERACTION WITH BSG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY282806 mRNA. Translation: AAP35025.1. AF175409 mRNA. Translation: AAD53278.1. AL391808, AL023586, Z98886 Genomic DNA. Translation: CAH72438.1. AL023586, AL391808, Z98886 Genomic DNA. Translation: CAI20566.1. Z98886, AL023586, AL391808 Genomic DNA. Translation: CAI22750.1. AL049672 mRNA. Translation: CAB41245.1. Different initiation. AL049673 mRNA. Translation: CAB41246.1. Different initiation.
IPI	IPI00001399.
RefSeq	NP_001035943.1. NM_001042478.1. NP_061324.1. NM_018836.3.
UniGene	Hs.25924.
3D structure databases
ProteinModelPortal	Q9UKB5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UKB5. 1 interaction.
STRING	Q9UKB5.
PTM databases
PhosphoSite	Q9UKB5.
Polymorphism databases
DMDM	74761984.
Proteomic databases
PeptideAtlas	Q9UKB5.
PRIDE	Q9UKB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378190; ENSP00000367432; ENSG00000196581. ENST00000378191; ENSP00000367433; ENSG00000196581.
GeneID	55966.
KEGG	hsa:55966.
UCSC	uc001alm.1. human.
Organism-specific databases
CTD	55966.
GeneCards	GC01P004714.
H-InvDB	HIX0028526.
HGNC	HGNC:30801. AJAP1.
HPA	HPA012157.
MIM	610972. gene.
neXtProt	NX_Q9UKB5.
PharmGKB	PA142672629.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15344.
GeneTree	ENSGT00510000048586.
HOGENOM	HBG716989.
HOVERGEN	HBG096853.
InParanoid	Q9UKB5.
OMA	ESNTFPG.
OrthoDB	EOG4KD6MS.
PhylomeDB	Q9UKB5.
Gene expression databases
ArrayExpress	Q9UKB5.
Bgee	Q9UKB5.
CleanEx	HS_AJAP1.
Genevestigator	Q9UKB5.
Family and domain databases
ProtoNet	Search...
Other
NextBio	61379.
SOURCE	Search...

Entry information

Entry name

AJAP1_HUMAN

Accession

Primary (citable) accession number: Q9UKB5
Secondary accession number(s): Q9Y229

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.