Q9UKA4 (AKA11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-kinase anchor protein 11
Short name=AKAP-11

Alternative name(s):
A-kinase anchor protein 220 kDa
Short name=AKAP 220
Short name=hAKAP220
Protein kinase A-anchoring protein 11
Short name=PRKA11

Gene names

Name:	AKAP11
Synonyms:	AKAP220, KIAA0629

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1901 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Note: Cytoplasmic in premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm.
Tissue specificity	Expressed in heart, brain, lung, liver, kidney, testis and ovary. Weakly expressed in skeletal muscle, pancreas and spleen.
Domain	RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Sequence similarities	Belongs to the AKAP110 family.
Sequence caution	The sequence BAA92117.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein kinase cascade Traceable author statement PubMed 10209101. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	protein kinase A binding Traceable author statement PubMed 10209101. Source: ProtInc protein phosphatase 1 binding Traceable author statement PubMed 10209101. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1901	1901	A-kinase anchor protein 11		PRO_0000064517
Regions
	Region	1650 – 1663	14	PKA-RII subunit binding domain
	Compositional bias	238 – 315	78	Ser-rich
	Compositional bias	1705 – 1767	63	Ser-rich
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.10 Ref.11
	Modified residue	422	1	Phosphoserine Ref.8
	Modified residue	433	1	Phosphoserine Ref.8 Ref.10
	Modified residue	448	1	Phosphoserine Ref.8 Ref.9
	Modified residue	1100	1	Phosphothreonine Ref.8 Ref.9 Ref.10
	Modified residue	1242	1	Phosphoserine Ref.10
	Modified residue	1337	1	Phosphoserine Ref.8
Natural variations
	Natural variant	721	1	S → C. Corresponds to variant rs2236364 [ dbSNP | Ensembl ].		VAR_020131
	Natural variant	1070	1	H → R. Corresponds to variant rs17063163 [ dbSNP | Ensembl ].		VAR_048207
	Natural variant	1410	1	L → F. Corresponds to variant rs17063167 [ dbSNP | Ensembl ].		VAR_048208
Experimental info
	Sequence conflict	1303	1	K → R in BAA92117. Ref.5
	Sequence conflict	1444 – 1469	26	LDPYR…ITKDA → CGPSVELSPWKWQTRCGREG NSWKRE in BAA92117. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9UKA4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 0893316C46A75672	FASTA	1,901	210,512
	10 20 30 40 50 60 MATFRNNHMK TKASVRKSFS EDVFQSVKSL LQSQKELCSV TAEDCLQQDE HANLTEVTFL 70 80 90 100 110 120 GFNEETDAAH IQDLAAVSLE LPDILNSLHF CSLNENEIIC MKNINKPLDI SSDPLNQSHP 130 140 150 160 170 180 SGMLCVMRVS PTSPRLRIDF IFSLLSKYAT GIRYTLDTFL HQKHQLETTD EDDDDTNQSV 190 200 210 220 230 240 SSIEDDFVTA FEHLEEEETS KPYNDGMNIT VLRSQCDAAS QTVTGHHLET HDLKILISSG 250 260 270 280 290 300 QQKSLAKPST SSVNVLGHKE LPSVKTSVTT SISEPWTQRS FYRSSNASDK DSDLQKTFFS 310 320 330 340 350 360 SSPAYSSESE CSSPSPVIFL DEEGYQKSLK AKLELPKIPV MKDDIEDSDS EVSEFFDSFD 370 380 390 400 410 420 QFDELEQTLE TCLFNKDPVI GKSSQRKGHK HGKSCMNPQK FKFDRPALPA NVRKPTPRKP 430 440 450 460 470 480 ESPYGNLCDA PDSPRPVKAS REDSGLFSPI RSSAFSPLGG CTPAECFCQT DIGGDRIHEN 490 500 510 520 530 540 HDSVYYTYED YAKSISCEVL GSVLRTHHTN TLSNINSIKH GENKTVTFKH GNLDQKNKSK 550 560 570 580 590 600 NKSLMIKDSI QKFAADLVEK SFGSAFKDLQ KGVSSCTNAL YHLAIKLTSS VLQMAFDELR 610 620 630 640 650 660 RQRAFSLKER AISGLANFLV SEALSNALKD LQYVKKQIFT NTVARFAADL AEELVFEGIM 670 680 690 700 710 720 EVCQFSYPQT PASPQCGSFD FEDKVVKLYA KDLSESVIQE AFIELSQVDV TFTTKAAVSV 730 740 750 760 770 780 STDNIKYVSA ESVVPSTQAV TFSPSFHNQA IMVTKPVQEY KKEYTVQQAL FCTSGIVTSI 790 800 810 820 830 840 PVPLAGSALL PYHISSTACQ AKAHLSSDDS NSNGDSAQVH IATKNREEKA ACLRNICLPS 850 860 870 880 890 900 EHNPGNQNDF KPTNDDIEMQ SSSKLPNDPA IISNFSAAVV HTIVNETLES MTSLEVTKMV 910 920 930 940 950 960 DERTDYLTKS LKEKTPPFSH CDQAVLQCSE ASSNKDMFAD RLSKSIIKHS IDKSKSVIPN 970 980 990 1000 1010 1020 IDKNAVYKES LPVSGEESQL TPEKSPKFPD SQNQLTHCSL SAAKDCVPEC KVSMVHGSSL 1030 1040 1050 1060 1070 1080 ETLPSCPAVT GQKSDLKESA KDQPLKKHNL NSTSLEALSF GQENPFPHSH TFSSTALTCV 1090 1100 1110 1120 1130 1140 DGLHVEDKQK VRDRNVIPDT PPSTPLVPSR ASSEWDIKKL TKKLKGELAK EFAPATPPST 1150 1160 1170 1180 1190 1200 PHNSSVGSLS ENEQNTIEKE EFMLKLMRSL SEEVESSESG ELPEVDVKSE HSGKKVQFAE 1210 1220 1230 1240 1250 1260 ALATHILSLA TEMAASHLDN KIIQEPKVKN PCLNVQSQRS VSPTFLNPSD ENLKTLCNFA 1270 1280 1290 1300 1310 1320 GDLAAEVITE AEKIAKVRNC MLFKQKKNSC YADGDEDYKV EEKLDIEAVV HPREVDPFIL 1330 1340 1350 1360 1370 1380 SLPPSSCMSG LMYKYPSCES VTDEYAGHLI QILKQEGGNS ELIMDQYANR LAYRSVKSGL 1390 1400 1410 1420 1430 1440 QEAAKTTKVQ CNSRMFPVPS SQVKTNKELL MFSNKEHHQE ADKKRQSKRN EGYFCKNQTC 1450 1460 1470 1480 1490 1500 ERTLDPYRNE VSQLYSFSTS LVHSITKDAK EELTASLVGL PKSLTDSCLF EKSGYEEDNE 1510 1520 1530 1540 1550 1560 CHVTPELPKS LQPSSQNHRF YHSTGSLNGY GCGDNVVQAV EQYAKKVVDD TLELTLGSTV 1570 1580 1590 1600 1610 1620 FRVSETTKSA DRVTYAEKLS PLTGQACRYC DLKELHNCTG NSSQHFFRQG SLASSKPASN 1630 1640 1650 1660 1670 1680 PKFSSRYQKS RIFHLSVPQI HVNLDKKAVL AEKIVAEAIE KAERELSSTS LAADSGIGQE 1690 1700 1710 1720 1730 1740 GASFAESLAT ETMTAAVTNV GHAVSSSKEI EDFQSTESVS SQQMNLSIGD DSTGSWSNLS 1750 1760 1770 1780 1790 1800 FEDEHQDESS SFHHLSESNG NSSSWSSLGL EGDLYEDNLS FPTSDSDGPD DKDEEHEDEV 1810 1820 1830 1840 1850 1860 EGLGQDGKTL LITNIDMEPC TVDPQLRIIL QWLIASEAEV AELYFHDSAN KEFMLLSKQL 1870 1880 1890 1900 QEKGWKVGDL LQAVLQYYEV MEKASSEERC KSLFDWLLEN A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Localization of a novel human A-kinase-anchoring protein, hAKAP220, during spermatogenesis." Reinton N., Collas P., Haugen T.B., Skalhegg B.S., Hansson V., Jahnsen T., Tasken K. Dev. Biol. 223:194-204(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 630-1901. Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1121-1469. Tissue: Placenta.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-422; SER-433; SER-448; THR-1100 AND SER-1337, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 AND THR-1100, MASS SPECTROMETRY.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-433; THR-1100 AND SER-1242, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF176555 mRNA. Translation: AAF07045.1. AL136527 Genomic DNA. Translation: CAB89419.1. AB014529 mRNA. Translation: BAA31604.2. AK002166 mRNA. Translation: BAA92117.1. Different initiation.
IPI	IPI00007411.
PIR	T00384.
RefSeq	NP_057332.1. NM_016248.3.
UniGene	Hs.105105.
3D structure databases
ProteinModelPortal	Q9UKA4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UKA4. 4 interactions.
STRING	9606.ENSP00000025301.
PTM databases
PhosphoSite	Q9UKA4.
Polymorphism databases
DMDM	13431310.
Proteomic databases
PaxDb	Q9UKA4.
PRIDE	Q9UKA4.
Protocols and materials databases
DNASU	11215.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000025301; ENSP00000025301; ENSG00000023516.
GeneID	11215.
KEGG	hsa:11215.
UCSC	uc001uys.2. human.
Organism-specific databases
CTD	11215.
GeneCards	GC13P042846.
HGNC	HGNC:369. AKAP11.
HPA	HPA039089.
MIM	604696. gene.
neXtProt	NX_Q9UKA4.
PharmGKB	PA24663.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG28523.
HOGENOM	HOG000033874.
HOVERGEN	HBG050470.
InParanoid	Q9UKA4.
KO	K16527.
OMA	SYAKDLS.
OrthoDB	EOG4ZW594.
PhylomeDB	Q9UKA4.
Gene expression databases
ArrayExpress	Q9UKA4.
Bgee	Q9UKA4.
CleanEx	HS_AKAP11.
Genevestigator	Q9UKA4.
GermOnline	ENSG00000023516. Homo sapiens.
Family and domain databases
InterPro	IPR008382. SPHK1-interactor_AKAP_110. [Graphical view]
PANTHER	PTHR10226. PTHR10226. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	AKAP11. human.
GenomeRNAi	11215.
NextBio	42687.
SOURCE	Search...

Entry information

Entry name

AKA11_HUMAN

Accession

Primary (citable) accession number: Q9UKA4
Secondary accession number(s): O75124, Q9NUK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.