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Protein

F-box only protein 3

Gene

FBXO3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.1 Publication

GO - Molecular functioni

GO - Biological processi

  • protein polyubiquitination Source: Reactome
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 3
Gene namesi
Name:FBXO3
Synonyms:FBX3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:13582. FBXO3.

Subcellular locationi

  • Nucleus

  • Note: Colocalizes with PML at the peripheries of nuclear bodies.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28040.

Polymorphism and mutation databases

BioMutaiFBXO3.
DMDMi145559474.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471F-box only protein 3PRO_0000119877Add
BLAST

Proteomic databases

EPDiQ9UK99.
MaxQBiQ9UK99.
PaxDbiQ9UK99.
PeptideAtlasiQ9UK99.
PRIDEiQ9UK99.

PTM databases

iPTMnetiQ9UK99.
PhosphoSiteiQ9UK99.

Expressioni

Gene expression databases

BgeeiQ9UK99.
CleanExiHS_FBXO3.
ExpressionAtlasiQ9UK99. baseline and differential.
GenevisibleiQ9UK99. HS.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SKP1P632082EBI-2509901,EBI-307486
tatP046083EBI-2509901,EBI-6164389From a different organism.

Protein-protein interaction databases

BioGridi117657. 16 interactions.
DIPiDIP-53688N.
IntActiQ9UK99. 6 interactions.
STRINGi9606.ENSP00000265651.

Structurei

Secondary structure

1
471
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi278 – 2814Combined sources
Beta strandi284 – 2929Combined sources
Helixi294 – 2963Combined sources
Turni299 – 3024Combined sources
Beta strandi303 – 31412Combined sources
Helixi320 – 3223Combined sources
Beta strandi324 – 33512Combined sources
Beta strandi340 – 3489Combined sources
Beta strandi360 – 38728Combined sources
Beta strandi391 – 40212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5HDWX-ray2.00A278-407[»]
ProteinModelPortaliQ9UK99.
SMRiQ9UK99. Positions 284-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 5647F-boxPROSITE-ProRule annotationAdd
BLAST
Domaini278 – 408131ApaGPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi418 – 45235Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi453 – 4564Poly-Arg
Compositional biasi463 – 4664Poly-Arg

Sequence similaritiesi

Contains 1 apaG domain.PROSITE-ProRule annotation
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOGENOMiHOG000232174.
HOVERGENiHBG005647.
InParanoidiQ9UK99.
KOiK10290.
OMAiDLGRYIQ.
PhylomeDBiQ9UK99.
TreeFamiTF329795.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UK99-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAMETETAP LTLESLPTDP LLLILSFLDY RDLINCCYVS RRLSQLSSHD
60 70 80 90 100
PLWRRHCKKY WLISEEEKTQ KNQCWKSLFI DTYSDVGRYI DHYAAIKKAW
110 120 130 140 150
DDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN
160 170 180 190 200
GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI
210 220 230 240 250
HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT
260 270 280 290 300
SYVKNVVSGG FPIIRDQIFR YVHDPECVAT TGDITVSVST SFLPELSSVH
310 320 330 340 350
PPHYFFTYRI RIEMSKDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG
360 370 380 390 400
EFPIISPGRV YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK IFNVAIPRFH
410 420 430 440 450
MACPTFRVSI ARLEMGPDEY EEMEEEEEEE EEEDEDDDSA DMDESDEDDE
460 470
EERRRRVFDV PIRRRRCSRL F
Length:471
Mass (Da):54,561
Last modified:April 17, 2007 - v3
Checksum:i5FDA81669264E0AD
GO
Isoform 2 (identifier: Q9UK99-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-415: EM → VS
     416-471: Missing.

Note: No experimental confirmation available.
Show »
Length:415
Mass (Da):47,530
Checksum:i9CA7AC918B28FCB0
GO
Isoform 3 (identifier: Q9UK99-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-40: Missing.
     414-415: EM → VS
     416-471: Missing.

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):46,975
Checksum:i221BD13D1AB4236E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281L → M in CAB66560 (PubMed:11230166).Curated
Sequence conflicti96 – 983IKK → VKR in CAB66560 (PubMed:11230166).Curated
Sequence conflicti164 – 1641A → T in BAA91991 (PubMed:14702039).Curated
Sequence conflicti227 – 2271D → G in CAB66560 (PubMed:11230166).Curated
Sequence conflicti239 – 2391F → L in CAB66560 (PubMed:11230166).Curated
Sequence conflicti378 – 3781G → E in CAB66560 (PubMed:11230166).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti221 – 2211V → I.
Corresponds to variant rs1402954 [ dbSNP | Ensembl ].
VAR_049037

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 405Missing in isoform 3. 1 PublicationVSP_039614
Alternative sequencei414 – 4152EM → VS in isoform 2 and isoform 3. 2 PublicationsVSP_024395
Alternative sequencei416 – 47156Missing in isoform 2 and isoform 3. 2 PublicationsVSP_024396Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001943 mRNA. Translation: BAA91991.1.
AK128454 mRNA. Translation: BAG54678.1.
AL136625 mRNA. Translation: CAB66560.1.
AC113192 Genomic DNA. No translation available.
AL049629 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68186.1.
CH471064 Genomic DNA. Translation: EAW68188.1.
CH471064 Genomic DNA. Translation: EAW68189.1.
BC046110 mRNA. Translation: AAH46110.1.
AF176702 mRNA. Translation: AAF03702.1.
CCDSiCCDS44566.1. [Q9UK99-2]
CCDS7887.1. [Q9UK99-1]
RefSeqiNP_036307.2. NM_012175.3. [Q9UK99-1]
NP_208385.1. NM_033406.2. [Q9UK99-2]
UniGeneiHs.406787.

Genome annotation databases

EnsembliENST00000265651; ENSP00000265651; ENSG00000110429. [Q9UK99-1]
ENST00000448981; ENSP00000408836; ENSG00000110429. [Q9UK99-2]
ENST00000530401; ENSP00000433781; ENSG00000110429. [Q9UK99-3]
GeneIDi26273.
KEGGihsa:26273.
UCSCiuc001muz.4. human. [Q9UK99-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001943 mRNA. Translation: BAA91991.1.
AK128454 mRNA. Translation: BAG54678.1.
AL136625 mRNA. Translation: CAB66560.1.
AC113192 Genomic DNA. No translation available.
AL049629 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68186.1.
CH471064 Genomic DNA. Translation: EAW68188.1.
CH471064 Genomic DNA. Translation: EAW68189.1.
BC046110 mRNA. Translation: AAH46110.1.
AF176702 mRNA. Translation: AAF03702.1.
CCDSiCCDS44566.1. [Q9UK99-2]
CCDS7887.1. [Q9UK99-1]
RefSeqiNP_036307.2. NM_012175.3. [Q9UK99-1]
NP_208385.1. NM_033406.2. [Q9UK99-2]
UniGeneiHs.406787.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5HDWX-ray2.00A278-407[»]
ProteinModelPortaliQ9UK99.
SMRiQ9UK99. Positions 284-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117657. 16 interactions.
DIPiDIP-53688N.
IntActiQ9UK99. 6 interactions.
STRINGi9606.ENSP00000265651.

PTM databases

iPTMnetiQ9UK99.
PhosphoSiteiQ9UK99.

Polymorphism and mutation databases

BioMutaiFBXO3.
DMDMi145559474.

Proteomic databases

EPDiQ9UK99.
MaxQBiQ9UK99.
PaxDbiQ9UK99.
PeptideAtlasiQ9UK99.
PRIDEiQ9UK99.

Protocols and materials databases

DNASUi26273.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265651; ENSP00000265651; ENSG00000110429. [Q9UK99-1]
ENST00000448981; ENSP00000408836; ENSG00000110429. [Q9UK99-2]
ENST00000530401; ENSP00000433781; ENSG00000110429. [Q9UK99-3]
GeneIDi26273.
KEGGihsa:26273.
UCSCiuc001muz.4. human. [Q9UK99-1]

Organism-specific databases

CTDi26273.
GeneCardsiFBXO3.
HGNCiHGNC:13582. FBXO3.
MIMi609089. gene.
neXtProtiNX_Q9UK99.
PharmGKBiPA28040.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOGENOMiHOG000232174.
HOVERGENiHBG005647.
InParanoidiQ9UK99.
KOiK10290.
OMAiDLGRYIQ.
PhylomeDBiQ9UK99.
TreeFamiTF329795.

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii26273.
PROiQ9UK99.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UK99.
CleanExiHS_FBXO3.
ExpressionAtlasiQ9UK99. baseline and differential.
GenevisibleiQ9UK99. HS.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Placenta and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  6. "PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation."
    Shima Y., Shima T., Chiba T., Irimura T., Pandolfi P.P., Kitabayashi I.
    Mol. Cell. Biol. 28:7126-7138(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CUL1; PML; RBX1 AND SKP1, UBIQUITINATION OF HIPK2.
  7. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-471 (ISOFORM 2).
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFBX3_HUMAN
AccessioniPrimary (citable) accession number: Q9UK99
Secondary accession number(s): B3KY16
, D3DR05, Q86X90, Q9H0V2, Q9NUX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 17, 2007
Last modified: July 6, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.